• 제목/요약/키워드: mycelial cultivation

검색결과 363건 처리시간 0.028초

볏짚을 이용(利用)한 사철느타리버섯의 배지(培地) 제조(製造) 및 종균재식(種菌栽植)에 관한 연구(硏究) (Studies on the Artificial Substrates with Rice Straw and the Spawning for Pleurotus florida in Korea)

  • 고승주;박용환;차동열
    • 한국균학회지
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    • 제9권2호
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    • pp.67-72
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    • 1981
  • 1. 사철느타리버섯(Pleurotus florida)는 퇴적시(堆積時)에 미강(米糠)을 볏짚건물중(建物重)의 5%로 첨가(添加)하여 퇴비(堆肥)더미 정상부(頂上部)의 온도(溫度)가 $60^{\circ}C$일 때 3회퇴적(回堆積)한 발효(醱酵)볏짚부지(部地)에서 가장 높은 자실체(子實體) 수확(收穫)을 얻을 수 있었다. 2. 사철느타리버섯의 종균재식(種菌栽植)재직량(栽直量)을 볏짚건물중(建物重)의 10%로, 부지(部地)와 혼합재식(混合栽植)하였을 때 자실체(子實體) 수량(收量)이 가장 높았다. 3. 한국(韓國)에서 사철느타리버섯의 재배기간(栽培期間)은 9월(月)부터 익년(翌年) 7월중순(月中旬)까지였다.

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느타리버섯(Pleurotus ostreatus) 재배(裁培)에 있어서 배지량(培地量) 및 종균(種菌) 재식량(載植量)이 자실체(子實體) 수량(收量)에 미치는 영향(影響) (The Effects of the Quantities of the Rice Straw Substrates and Spawn on the Yield of Oyster Mushroom Pleurotus ostreatus)

  • 박용환;장학길;고승주
    • 한국균학회지
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    • 제5권1호
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    • pp.1-5
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    • 1977
  • 볏짚을 이용(理容)한 느타리버섯[Pleurotus ostreaus (Fr.) $Quacute{e}l.$] 재배(栽培)에 있어서 배지량(培地量) 및 종균재식량(種菌裁植量)이 자실체(字實體) 수량(收量)에 미치는 영향(影響)을 보고자 시험(試驗)을 수행(遂行)한 결과, 볏짚량(量)을 $90kg/3.3m^2$까지 증가(增加)시켰을 때 자실체(字實體) 수량(收量)은 $102kg/3.3m^2$으로 가장 높았고, 종균(種菌)을 표면(表面) 재식(裁植)할 경우(境遇) 종균량(種菌量)은 $8kg/3.3m^2$까지 증가(增加)시키므로서 증수(增收)되었으며 균사생장(菌絲生長) 기간(期間)도 단축(短縮)되었다.

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팽이버섯의 배지조성에 따른 균사 배양 및 자실체 수량 비교 (Comparison of mycelial growth and fruiting bodies yield according to substrate in Flammulina velutipes)

  • 정종천;이찬중;문지원
    • 한국버섯학회지
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    • 제12권2호
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    • pp.117-121
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    • 2014
  • 본 시험은 팽이버섯 병재배용 배지의 혼합수준에 따른 전탄소(T-C), 전질소(T-N) 함량과 균배양기간, 버섯발생기간, 자실체 수량 등 재배적 특성을 조사하였다. 배지재료의 혼합비율을 달리하였을 때 팽이버섯의 경우 [콘코브75+미강20+건비지5] 배지에서 자실체 수량이 190.5 g/850 ml로 가장 높았으나(20.4% 증가), 균 배양기간이 28일로 대조구보다 8일 지연되었다(대조구 [미송톱밥75+미강25]: 수량 158.2g, 배양기간 20일). [미송톱밥75+미강20+건비지5] 배지는 팽이버섯의 자실체 수량이 172.7 g(9.1% 증가), 균 배양기간이 21일로 대조구보다 1일 지연되었다.

곡물배지를 이용한 번데기동충하초 재배기술 (Establishment of artificial cultivation technique of Cordyceps militaris using a grain media)

  • 유영진;서경원;이공준;최동칠;최정식;박정식;성재모
    • 한국버섯학회지
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    • 제5권2호
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    • pp.81-85
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    • 2007
  • 본 연구는 번데기동충하초를 곡물배지를 이용하여 자실체를 형성하는 실험으로 액체종균제조에 적합한 조건은 맥아추출배지를 농도 0.7%, pH 5~5.5,으로 조정하였을 때 균사의 건중량은 $3.9{\sim}4.1mg/m{\ell}$로 양호 하였다. 자실체 발생 최적 환경조건은 습도 95%, 온도 $17^{\circ}C$, 조도 600 lx, 이산화탄소농도 500ppm하였을 때, 곡물배지 일반현미와 흑미에서 24.3g/병, 20.5g/병의 자실체가 형성되었다.

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Root Rot of Balloon Flower (Platycodon grandiflorum) Caused by Fusarium solani and Fusarium oxysporum

  • Jeon, Chi Sung;Kim, Gyoung Hee;Son, Kyeong In;Hur, Jae-Seoun;Jeon, Kwon-Seok;Yoon, Jun-Hyuck;Koh, Young Jin
    • The Plant Pathology Journal
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    • 제29권4호
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    • pp.440-445
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    • 2013
  • Balloon flower (Platycodon grandiflorum) is a kind of mountain herbs whose roots have restorative properties and the cultivating acreage of balloon flower has been steadily increasing in Korea. More frequent rain and high amount of rainfalls as a result of climate changes predisposed balloon flower to the outbreaks of root rot at high-density cultivation area in recent years. Root crowns were usually discolored into brown to blackish brown at first and the infected plants showed slight wilting symptom at early infection stage. Severely infected roots were entirely rotted and whole plants eventually died at late infection stage. The overall disease severities of root rot of balloon flower were quite variable according to the surveyed fields in Jeonnam, Gyeongnam and Jeju Provinces, which ranged from 0.1% to 40%. The root rot occurred more severely at the paddy or clay soils than the sandy soils and their severities were much higher at lowland than upland in the same localty. The disease increased with aging of the balloon flower. The causal fungi were identified as Fusarium solani and F. oxysporum on the basis of their mycological characteristics. The optimum temperature ranges of their mycelial growths was found to be $24^{\circ}C$. The pathogenic characters of F. solani and F. oxysporum treated by artificial wounding inoculation on healthy roots of balloon flower revealed that F. solani was more virulent than F. oxysporum. This study identified the causal agents of root rot of balloon flower as Fusarium solani and F. oxysporum, probably for the first time.

Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes

  • Kang, Dae-Sun;Min, Kyong-Jin;Kwak, A-Min;Lee, Sang-Yeop;Kang, Hee-Wan
    • The Plant Pathology Journal
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    • 제33권3호
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    • pp.264-275
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    • 2017
  • The spent mushroom substrate (SMS) of Lentinula edodes that was derived from sawdust bag cultivation was used as materials for controlling Phytophthora blight disease of pepper. Water extract from SMS (WESMS) of L. edodes inhibited mycelial growth of Phytophthora capsici, suppressed Phytophthora blight disease of pepper seedlings by 65% and promoted growth of the plant over 30%. In high performance liquid chromatography (HPLC) analysis, oxalic acid was detected as the main organic acid compound in WESMS and inhibited the fungal mycelium at a minimum concentration of 200 mg/l. In quantitative real-time PCR, the transcriptional expression of CaBPR1 (PR protein 1), CaBGLU (${\beta}$-1,3-glucanase), CaPR-4 (PR protein 4), and CaPR-10 (PR protein 10) were significantly enhanced on WESMS and DL-${\beta}$-aminobutyric acid (BABA) treated pepper leaves. In addition, the salicylic acid content was also increased 4 to 6 folds in the WESMS and BABA treated pepper leaves compared to water treated leaf sample. These findings suggest that WESMS of L. edodes suppress Phytophthora blight disease of pepper through multiple effects including antifungal activity, plant growth promotion, and defense gene induction.

NaCl 함량에 따른 내염성 느타리버섯 선발과 재배적 특성 (Cultural characteristics and selection of saline tolerant Pleurotus ostreatus at different NaCl concentration medium)

  • 최종인;지정현;하태문;주영철
    • 한국버섯학회지
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    • 제3권2호
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    • pp.65-70
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    • 2005
  • 국내의 NaCl 함유한 부존자원중 버섯배지화 하기 위하여 내염성 느타리버섯 균주를 선발하고, 선발된 균주를 NaCl 함유 톱밥배지에서 재배하여 생장 및 형태적 특성을 조사한 결과는 다음과 같다. 가. 느타리 버섯 64품종중 NaCl이 3% 첨가한 PDA 배지에서 김제 10호, 농기2-1호, 명월, 병느타리1호, 부평소엽1호, 삼복, 춘추2호 등이 다른품종에 비해 균사생장 및 균사밀도가 양호하였다. 나. 명월은 NaCl 0.5%, 김제10호, 부평소엽1호는 1.0%, 농기2-1호, 병느타리1호, 삼복, 춘추느타리 2호는 1.5%까지 자실체 형성이 가능하였다. 다. 톱밥배지에서 염농도가 높아질수록 배양기간이 길어졌으며 대가 짧아지고 가늘어졌으며 수량이 감소했다. 라. 배지내에 NaCl 함량이 높아질수록 버섯의 $K_2O$, CaO 흡수능은 감소되었으며 자실체내의 NaCl함량은 배지내 NaCl농도가 높을수록 증가하였다.

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차신고버섯 신품종 "다산"의 특성 (Characteristics of new commercial strain "Dasan" in Agrocybe chaxingu)

  • 이광재;박영학;함헌주;음원식;주진호;김경희
    • 한국버섯학회지
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    • 제8권1호
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    • pp.6-10
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    • 2010
  • 볏짚버섯속 유망버섯인 차신고버섯은 현재까지 1종만이 품종으로 등록되어 있는 실정으로 국내 고유품종 확보 및 버섯재배품종 다양화에 기여하고자 품종육성 연구를 수행한 결과, 차신고버섯 신품종 '다산'의 균사생장적온은 $25{\sim}30^{\circ}C$이었으며 탄소원으로 glucose(maltose), 질소원으로 ammonium chloride(pepton)를 사용 시 균사생장 및 균사밀도가 우수하였고 탄소원과 질소원 무첨가 시 균사는 생장하였으나 균사의 밀도가 비교적 낮았다. 또한 버섯발생 특성은 신품종 "다산"이 초발이 소요일수, 수확 소요일수 및 수량은 각각 12~13일, 6~8일, 118.2g 으로 진향품종에 비해 다소 발이가 늦은 편이나 수량성은 양호하였다. 또한 "다산" 품종은 갓의 색깔은 대조품종 보다 진하고 갓의 직경은 작았으며 대의 길이는 길고 갓의 직경이 작은 자실체 특성을 가지고 있었다.

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대추 역병균의 Phytophthora citricola의 동정 (Phytophthora citricola, a Causal Agent of Jujube (Zizyphus jujuba) Fruit Rot)

  • 지형진;임양숙;정기채;조원대
    • 한국식물병리학회지
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    • 제14권5호
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    • pp.402-407
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    • 1998
  • Phytophthora rot on jujube fruit has occurred at several cultivation areas in Kyung-buk and Kyung-nam provinces. Symptoms consisted of brownish to reddish rot on fruits resulting in early drop or mummification. The causal fungus isolated from infected fruits and adjacent leaf stalks was identified as Phytophthora citricola, which has never been reported in Korea. Sporangia were semi-papillate, noncaducous and highly variable in shapes. Plerotic oospores with paragynous antheridia were abundant is single cultures. Sporangia of two isolates were measured as 38-76$\times$20-40 ${\mu}{\textrm}{m}$ and averaged 51.4$\times$27.0 and 55.6$\times$36.0 ${\mu}{\textrm}{m}$. Oogonia were ranged from 26 to 36 ${\mu}{\textrm}{m}$ and averaged 31.3 and 32.0 ${\mu}{\textrm}{m}$. Colony pattern was slightly radiated with sparse aerial mycelia on common media. Minium, optimum and maximum temperatures for mycelial growth were recorded at 7, 25, and 32$^{\circ}C$, respectively. Among tested media, 10% V8A was the best and $25^{\circ}C$ was better than 15$^{\circ}C$ for oospore formation of the fungus. The jujube isolates of P. citricola were readily differentiated from other closely related species in the genus, namely; P. nicotianae, P. citrophthora, P. cactorum, P. capsici, and P. plalmivora on the basis of PCR-RFLP of r-DNA. The fungus showed strong pathogenicty to jujube, apple, pear, orange, persimmon and eggplant, and relatively weak to citron, tomato, pepper and cucumber. In this study, P. citrocola is firstly identified and jujube fruit rot caused by the fungus is recorded as a new disease in Korea.

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Phytophthora nicotianae var. nocotianae에 의한 딸기 역병의 발생 (Occurrence of Phytophthora Rot of Strawberry Caused by Phytophthora nicotianae var. nicotianae)

  • 송주희;노성환;하주희;정연화;문병주
    • 한국식물병리학회지
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    • 제14권5호
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    • pp.445-451
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    • 1998
  • A severe Phytophthora rot of strawberry caused by a species of Phytophthora has been widely occurred at major cultivation areas of Kimhae on August in 1997. Incidence of the disease was obtained in the range of 69.2~83.6% in surveyed 4 fields and showed an average of 75.2%. A species of Phytophthora was mostly isolated from the crown of infected strawberry plants and all the isolates were identified as P. nicotianae var. nicotianae (=P. parasitica). The fungus showed strong pathogenicity on strawberry by inoculation test. As a result of the leaf inoculation using mycelial disks of the fungus, both leaves and petioles were darkly browned, and were finally blighted. As a result of the root inoculation of zoospore suspension, both roots and crowns were rotten with dark brown. Although the fungus produced sporangia either on V-8 juice agar medium or liquid medium, the sporangia observed on the liquid medium appeared to be broadly turbinate and noncaducous. Moreover the fungus cultured on the liquid medium often produced sporangia having two papilla. The number of zoospores in sporangia was found to be ranged from 3 or 4 to as many as 20 or 25. In addition, the released zoospore from the sporangium became the cystospore during the prolonged culture of the fungus. The sporangia were measured as av. 49$\times$35 ${\mu}{\textrm}{m}$ with l/b ratio of 1.43. All isolates from crowns were heterothallic and A1 mating type since oospores were abundantly formed on clarified V-8 juice agar by dual culture with P. capsici A2 mating type. Aplerotic oospores were sized 24-26 ${\mu}{\textrm}{m}$. Antheridia were always amphigynous and recoreded an average of 12$\times$10 ${\mu}{\textrm}{m}$. Hyphal swlling were easily observed, and terminal or intercalary chlamydospores were abundantly formed on V-8 juice agar as well as in C/Z solution and sized av. 28.2 ${\mu}{\textrm}{m}$. This is the first report of Phytophthora rot of strawberry in Korea.

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