• 한국환경농학회지
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• 제36권4호
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• pp.249-255
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• 2017

BACKGROUND: Globally, mulberry (Morus sp.) is exploited for feeding leaf to silkworms in order to obtain silk fiber or for animal feedstock production. Also, mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a newincome crop. Mulberry leaves and fruits are used for the health benefits of human beings. Mulberry contains various bioactive components, such as alkaloids and flavonoids. Mulberry flavonoids are an important part of the diet because of their effects on human nutrition. The flavonoids in mulberry leaf and fruit of 'Suhyang'(Morus alba L.) were determined. METHODS AND RESULTS: Flavonoids for mulberry leaf and fruit of 'Suhyang' were analysed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS)technique. An UPLC-DAD-QTOF/MS system was used, and identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from LC spectra, MS ions, and MS/MS fragments. The mulberry leaf (16 flavonoids) and fruit (9 flavonoids) were isolated and analyzed from Suhyang using UPLC-DAD-QTOF/MS chromatogram. To the best of our knowledge, Quercetin 3-O-(6"-O-malonyl) glucoside and quercetin 3-O-rutinoside (rutin) was detected on the highest content in leaf and fruit, respectively and further research will be devoted to evaluate their biological activity. CONCLUSION: Obtaining information about the concentration of functional materials in mulberry leaves could contribute to the development and promotion of processed, functional products and offer possible industrial use of 'Suhyang', holding promises to enhance the overall profitability of sericulture.

• 한국약용작물학회지
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• 제15권2호
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• pp.120-127
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• 2007

The objective of this research was to evaluate the ability of water and ethanol extracts from mulberry fruit (Morus alba L.) to influence the inhibitory activity of angiotensin converting enzyme (ACE) and xanthine oxidase(XOase). The total phenol contents and sixteen phenolic compounds were investigated in water and ethanol extracts. In order to understand the factors responsible for the potent antioxidant and antihypertensive ability of mulberry, it has been evaluated for anti-oxidative activity using Fenton's reagent/ethyl linoleate system and for free radical scavenging activity using the 1,1-diphenyl-2-picryl hydrazyl free radical generating system. The total phenol contents and total of phenolic compounds in ethanol extract showed higher levels than water extract in mulberry fruit six phenolic compounds (chlorogenic acid, narigin, syringic acid, quercetin, naringenin, kampferol) has a higher individual phenolic compound content in the 60% ethanol extraction than 80% ethanol extract. The inhibitory activity on angiotensin converting enzyme (ACE) were highest in 80% ethanol extract (9.0%). Also, activity of xanthine oxidase(XOase) inhibition appeared highest in 80% ethanol extracts and correlated well with the total phenolic content, which was modulated by the concentration of individual phenolic compounds. This result revealed, that strong biological activity was caused by specific phenol compound contents. Utilization of water and ethanol extracts from mulberry fruit are expected to be good candidate for development into source of free radical scavengers and anti-hypertentive activity

• Journal of Applied Biological Chemistry
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• 제62권2호
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• pp.111-122
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• 2019

The mulberry tree (Morus alba L.) has been traditionally used in Chinese medicine to treat inflammatory diseases. We investigated the effects of bioconversion on different components of the mulberry tree, and determined changes in the physiological activities. Ethyl acetate-soluble fractions of five different segments (fruit, Mori Fructus; leaf, Mori Folium; twig, Mori Ramulus; root, Mori Cortex; and mistletoe, Loranthi Ramulus) of the mulberry tree show enhanced anti-oxidant effects in the 2,2-diphenyl-1-picrylhydrazyl, and 2,2'-azinobis-(3-ethylvenzothiazoline-6-sulfonic acid) assays, and enhanced anti-inflammatory effects of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW 264.7 macrophages, after being treated with a crude enzyme extract from Aspergillus kawachii, in the following order of activity: Mori Folium>Mori Cortex>Mori Ramulus>Mori Fructus>Loranthi Ramulus. Ethyl acetate- soluble fraction of mulberry leaves (Mori Folium) that underwent bioconversion was most effective, and was devoid of any cytotoxicity. The fraction was also effective against mRNA expression of LPS-induced pro-inflammatory cytokines, such as inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis $factor-{\alpha}$, $interleukin-1{\beta}$, and interleukin-6. In addition, the fraction was effective in LPS-induced phosphorylation of mitogen-activated protein kinases and IKK, and $I{\kappa}B$ degradation, followed by translocation of the nuclear $factor-{\kappa}B$ from the cytoplasm to the nucleus. Thus, bioconversion increased the anti-oxidative and anti-inflammatory activities of the mulberry leaf.

• 한국잠사곤충학회지
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• 제47권2호
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• pp.93-98
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• 2005

야생 뽕나무의 자원화 및 이용기술을 개발하기 위해 강원도 원주, 인제, 양양 3개 지역에서 성숙 단계별로 채취한 야생 뽕나무를 대상으로 뽕잎과 오디의 항산화능을 비교분석 하였다. 항산화능 측정장치(munilum L-100, ABCD GmbH) 및 ARAW-KIT(anti-radical ability of water-soluble substance)를 사용하였으며, ascorbic acid를 표준물질로 사용하여 calibration curve를 작성하였다. 각각의 지역에서 채취한 야생뽕의 형태학적 특성을 조사한 결과, 원주 지역 채집 개체는 백상형(Morus alba L.), 인제 지역 채집 개체는 산상형(Morus bombycis Koidz) 및 양양 지역 채집 개체는 노상형(Morus Lhou (Ser.) Koidz)의 뽕나무로 분류되었다. 강원도 원주, 인제 및 양양 지역에서 자생하고 있는 야생 뽕나무의 뽕잎에 대한 전체적인 항산화능을 분석한 결과 춘기 5개엽기의 어린 뽕잎의 항산화능이 가장 높았으며, 시기가 경과함에 따라 뽕잎의 항산화능은 점차 감소하는 경향이었다. 그러나 개체마다 각각의 특성을 나타냈다. 오디가 결실된 야생 뽕나무 중 오디의 수량성 및 크기 등 착과상태를 고려하여 이용가치가 있을 것으로 예상되는 개체는 No 7, 8, 10이었다. 산상형의 특징을 갖는 No. 7 개체 뽕나무는 뽕잎의 항산화능이 높을 뿐 만 아니라 뽕나무의 생육 상태 및 오디의 결실 상태도 양호했으므로 기능성 및 오디생산용 육종 유전자원으로 이용할 가치가 있는 것으로 판단되었으며, No. 8 및 No. 10 개체 뽕나무는 오디 생산을 위한 유전자원으로 이용할 수 있을 것으로 보인다.

• 한국식품영양과학회지
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• 제44권6호
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• pp.823-831
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• 2015

뽕나무를 이용한 당뇨, 고혈압 및 노화 개선용 고부가가치의 기능성 소재 및 제품을 개발하기 위한 연구의 일환으로 먼저 뽕나무 부위별(오디, 상엽, 상지, 상백피) 물 및 에탄올 추출물을 제조하여 그들의 항산화, 항당뇨, 항염증 및 미백 활성을 in vitro assay를 이용하여 각각 측정하였으며, 아울러 각부위별 주된 기능성 성분의 함량을 HPLC를 이용하여 측정한 결과는 다음과 같다. 먼저 뽕나무 부위별 물 및 에탄올 추출물의 생리활성 및 기능성 성분의 함량을 측정한 결과 거의 대부분 에탄올 추출물이 물 추출물보다 활성과 함량이 높았다. 잠상상물 중 오디, 상백피 및 상지 에탄올 추출물이 가장 강한 항산화($IC_{50}=128.4{\mu}g/mL$), ${\alpha}$-glucosidase($IC_{50}=12.0{\mu}g/mL$) 및 lipoxygenase($IC_{50}=36.3{\mu}g/mL$)와 tyrosinase($IC_{50}=410.3{\mu}g/mL$) 저해 활성을 각각 나타내었다. 한편 오디에는 anthocyanin(cyanidin 3-glucoside: 213.20 mg/100 g), chlorogenic acid(123.61 mg/100 g) 및 rutin(66.51 mg/100 g)이, 상엽에는 다량의 chlorogenic acid(514.97 mg/100 g)가 함유되어 있었으며, 특히 에탄올 추출물에는 물 추출물에 존재하지 않는 항당뇨 및 항고혈압성 quercetin 3-O-(6-O-malonyl)glucoside(143.25 mg/100 g) 및 kaempferol 3-O-(6-O-malonyl)glucoside(30.25 mg/100 g) 성분이 존재하였다. 상지 및 상백피에는 항염증 및 항노화성 oxyresveratrol 성분이 주성분으로 존재하였으며, 특히 상지 추출물에는 oxyres-veratrol glycoside(48.90 mg/100 g) 및 aglycone(21.88 mg/100 g) 성분이 다 존재하는 반면, 상백피에는 oxyresveratrol glycoside(724.05 mg/100 g)가 거의 대부분 차지하고 있었다. 또한 상백피(223.90 mg/100 g) 및 상엽(86.07 mg/100 g)에는 항당뇨 및 항고혈압성 ${\gamma}$-aminobutyric acid 및 1-deoxynojirimycin 함량이 가장 많이 함유되어 있었다. 이상의 결과로부터 항산화성이 강한 오디 및 상엽 추출물과 항당뇨, 항염증 및 미백 활성이 높은 상지 및 상백피 추출물을 적절히 혼합한 성인병 예방용 고부가가치 잠상산물 복합제품의 개발이 요구되고 있으며, 현재 항당뇨 및 항염증 활성이 강한 상지 및 상백피 물 추출물을 이용한 기능성 음료 및 와인 개발과 더불어 항염증 및 미백 활성이 높은 상지 및 상백피 에탄올 추출물을 이용한 여드름 개선 및 미백 한방화장품 개발에 관한 연구가 진행되고 있다.

• 한국식품영양과학회지
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• 제37권1호
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• pp.124-128
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• 2008

본 연구는 DAD/UV HPLC를 이용하여 뽕잎과 오디(Morus alba L.)에 함유되어 있는 stilbenoids의 조성과 함량을 분석하고자 수행되었다. 뽕잎과 오디추출물을 DAD를 이용하여 최대흡광파장을 탐색한 결과 308 nm로 나타났으며, stilbenoids 조성이 오디에는 resveratrol, piceatannol, rhapontigenin, astringin, pterostilbene, piceid, rhaponticin, 뽕잎에는 resveratrol, rhapontigenin, pterostilbene, piceid이 함유된 것으로 나타났다. 오디와 뽕잎의 총 stilbenoids 함량은 $609.15{\pm}7.24$ mg/100 g d.w.와 $188.57{\pm}1.70$ mg/100 g d.w.였으며, rhaponticin은 오디($389.26{\pm}5.22$ mg/100 g d.w.)와 뽕잎($99.17{\pm}2.79$ mg/100 g d.w.) 모두 가장 많은 함량이 함유되어 있었다. Astringin과 piceatannol은 오디에서만 검출되었으며 vitisin A는 모두 검출되지 않았다. Piceid와 rhaponticin은 이의 비배당체인 resveratrol과 rhapontigenin보다 과량 함유되어 있어 비배당체보다는 배당체의 함량이 높은 것으로 나타났다.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.41-49
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• 2016

We bred a mulberry cultivar named Shimgang, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four local places(Suwon, Chuncheon, Buan and Jinju) for five years from 2011. This variety 'Shimgang' belongs to Morus Microphylla Buckl. selected from seedlings imported from USA. Shimgang was resistant to popcorn disease and high yielding variety in fruit productivity by 111% compared to control cultivar 'Shimheung(Morus alba L.)' for three years. Although fruits of 'Shimgang' was smaller in size and lower in sugar content of mulberry fruits, it showed stronger resistance than that of 'Shimheung'. It is adaptable to every where except the places where cold and late frost damage happen frequently.

• 한국약용작물학회지
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• 제22권4호
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• pp.262-269
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• 2014

Complex of mulberry extract (CME) is composed of extracts of mulberry (Morus alba L.) fruit, mulberry leaves and black beans (Glycine max (L.) Merr.). In this study, we investigated prevention effects of CME on degenerative arthritis. The $SC_{50}$ value of DPPH radical scavenging by CME was $158.49{\pm}11.35{\mu}/mL$. We found that CME significantly reduced the production of nitric oxide (NO) and protein expression of cyclooxygenase-2 (COX-2) in RAW 264.7 cells which were activated by LPS. Experiments using animal model of degenerative arthritis showed that CME (400 mg/kg body weight) inhibited the production of TNF-${\alpha}$ (77.5%) and IL-$1{\beta}$ (95.0%). Furthermore, it was observed that CME reduced to 85.9% of paw edema induced by carrageenan. These results suggest that CME could improve degenerative arthritis.

• International Journal of Industrial Entomology and Biomaterials
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• 제42권2호
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• pp.25-32
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• 2021

Mulberry fruit (Morus alba L.) contains phytochemicals, including 1-deoxynojirimycin, quercetin-glucoside, kaempferol-glucoside, and anthocyanins, which have antioxidant effects. In this study, mulberry fruit extract was prepared at various temperatures (25-100℃) and water/ethanol solvent concentrations (0%-100% ethanol). Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism (CD) data indicated that the content of bioactive compounds such as polyphenols and flavonoids was lower in 100% ethanolic extracts than in 30%-50% ethanolic extracts. Radical scavenging activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-di-3-ethylbenzthiazoline sulfonic acid (ABTS) assays was highly correlated with polyphenol and flavonoid content. In conclusion, 30%-50% ethanolic extracts contained the highest contents of bioactive compounds and exhibited high levels of radical scavenging activity. These findings may inform the use of mulberry fruit extract as a functional food.

• The Korean Journal of Physiology and Pharmacology
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• 제15권6호
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• pp.353-361
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• 2011

In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial function were assessed by LDH release and MTT reduction assays, respectively. A time-course study of OGD-induced cell death of primary cortical neurons at 7 days in vitro (DIV) indicated that neuronal death was OGD duration-dependent. It was also demonstrated that OGD for 3.5 h resulted in approximately 50% cell death, as determined by the LDH release assay. Treatments with mulberry C3G fraction prevented membrane damage and preserved the mitochondrial function of the primary cortical neurons exposed to OGD for 3.5 h in a concentration-dependent manner. Glutamate-induced cell death was more pronounced in DIV-9 and DIV-11 cells than that in DIV-7 neurons, and an application of $50{\mu}M$ glutamate was shown to induce approximately 40% cell death in DIV-9 neurons. Interestingly, treatment with mulberry C3G fraction did not provide a protective effect against glutamate-induced cell death in primary cortical neurons. On the other hand, treatment with mulberry C3G fraction maintained the mitochondrial membrane potential (MMP) in primary cortical neurons exposed to OGD as assessed by the intensity of rhodamine-123 fluorescence. These results therefore suggest that the neuroprotective effects of mulberry C3G fraction are mediated by the maintenance of the MMP and mitochondrial function but not by attenuating glutamate-induced excitotoxicity in rat primary cortical neurons.