• 대한의용생체공학회:의공학회지
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• 제44권3호
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• pp.176-181
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• 2023

During cancer treatment, the patient's response to drugs appears differently at the cellular level. In this paper, an image-based cell phenotypic feature quantification and key feature selection method are presented to predict the response of patient-derived cancer cells to a specific drug. In order to analyze the viability characteristics of cancer cells, high-definition microscope images in which cell nuclei are fluorescently stained are used, and individual-level cell analysis is performed. To this end, first, image stitching is performed for analysis of the same environment in units of the well plates, and uneven brightness due to the effects of illumination is adjusted based on the histogram. In order to automatically segment only the cell nucleus region, which is the region of interest, from the improved image, a superpixel-based segmentation technique is applied using the fluorescence expression level and morphological information. After extracting 242 types of features from the image through the segmented cell region information, only the features related to cell viability are selected through the ReliefF algorithm. The proposed method can be applied to cell image-based phenotypic screening to determine a patient's response to a drug.

• 생약학회지
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• 제44권4호
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• pp.326-331
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• 2013

A Korean folk medicine, "SinKyungCho" has been used to cure common cold and rheumatis. While this crude drug has similar morphological feature to that of Rubia species, the botanical origin has never been studied pharmacognostically. Therefore, in order to clarify the botanical origin of "SinKyungCho", the morphological and anatomical characteristics of Rubia species growing in Korea, i.e. R. akane, R. chinensis var. glabrescens, R. cordifolia var. pratensis, R. cordifolia var. sylvatica were studied. Especially, differing from the other three species, R. cordifolia var. pratensis has 2-4 cork cell layers under the endodermal cell layer. R. cordifolia var. sylvatica showed the largest number of parenchyma cell layers in cortex by 8-17. While the average ratio of stele to root transverse section is over 45% for R. akane and R. cordifolia var. pratensis, the ratio is under 45% for R. chinensis var. glabrescens and R. cordifolia var. sylvatica. According to these morphological criteria, the folk medicine "SinKyungCho" from Korean traditional market was proved to be the underground part of R. akane.

• 대한전기학회논문지:전력기술부문A
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• 제48권9호
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• pp.1167-1174
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• 1999

In this paper, we present a new technique for the local decomposition of convex structuring elements for morphological image processing. Local decomposition of a structuring element consists of local structuring elements, in which each structuring element consists of a subset of origin pixel and its eight neighbors. Generally, local decomposition of a structuring element reduces the amount of computation required for morphological operations with the structuring element. A unique feature of our approach is the use of linear integer programming technique to determine optimal local decomposition that guarantees the minimal amount of computation. We defined a digital convex polygon, which, in turn, is defined as a convex structuring element, and formulated the necessary and sufficient conditions to decompose a digital convex polygon into a set of basis digital convex polygons. We used a set of linear equations to represent the relationships between the edges and the positions of the original convex polygon, and those of the basis convex polygons. Further. a cost function was used represent the total processing time required for computation of dilation/erosion with the structuring elements in a decomposition. Then integer linear programming was used to seek an optimal local decomposition, that satisfies the linear equations and simultaneously minimize the cost function.

• ALGAE
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• 제18권4호
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• pp.239-253
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• 2003

The morphotaxonomic characters of the genus Spirogyra were investigated to clarify their taxonomic category and the variation range among species on the basis of comparative morphological and numerical analyses by unialgal cultures and field samples collected from various freshwater habitats in Korea. 25 characters selected on morphological feature of the species were examined on 568 individuals for morphological comparisons and numerical analyses. Width, length and their length/width ratio of vegetative cell, shape of septum, chloroplast number, maximum width, width, length and their length/width ratio of female gametangium, length of male gametangium, size and shape of zygospore, and cell wall ornamentation of the spore showed a comparatively high vector in principal component analysis. In cluster analysis, 15 taxa analysed were divided into 8 major groups by the average taxonomic distance 1.0 level. Considering the morphology and numerical analysis, Spirogyra crassoidea could not be recognized as an independent species, therefore it was treated as a variety of S. ellipsospora. S. koreana (nom. invalidum), recognized as a new taxon, is under the investigation for its clear taxonomic category.

• 전기전자학회논문지
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• 제7권2호
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• pp.210-217
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• 2003

본 논문에서는 수리형태학에 기반한 새로운 특징값 추출 방법을 제안하였다. 수리형태학적 패턴 분포함수에 의한 수리형태학적 공간주파수를 소개하고 이를 새로운 특징인 평균높이를 정의하는데 적용하였다. 평균높이는 내용기반 이미지 검색을 위한 인덱스 키를 생성하는데 사용되는 특징값을 정의하기 위하여 사용하였다. 제안한 방법의 우수성을 다양한 영상에 대한 실험을 통하여 증명하였다. 또한 기존의 검색방법은 검색하는 이미지 데이터마다 에지 검출단계에서 적절한 문턱값에 의한 이진화 처리가 요구되어 지나 제안한 방법은 이진화가 필요 없어서 그 효용성이 크다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.78-78
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• 2019

Gnaphalium L. is a small herbaceous genus comprising up to 80 species in worldwide (Asia, North to South America, Africa, and Oceania). This genus is highly polymorphic which embrace uncommon broad morphological boundary, and thus further studies were needed to proper taxonomic delimitations for the genus and its relatives. Gnaphalium uliginosum L. was usually found in moist sites such as margins of lake, pond, reservoir, stream banks and paddy field. This squat plant is solely known species in Korean Gnaphalium. During the revisionary study of the tribe Gnaphalieae (Asteraceae) in Korea, however, we found several materials in domestic herbaria (e.g., SNU, KWNU) that identified as G. uliginosum or Gamochaeta pensylvanica (Willd.) Cabrera collected from central to northern Korea, but clearly differ to the morphology of G. uliginosum. The external morphology of the materials is seemingly the only feature at odds with G. uliginosum. However, its morphological characters such as tall erected stems (ca. 30cm), hairs on seeds and whitish tomentose hairs on the whole plants are easily distinguished from G. uliginosum, and rather it looks like G. tranzschelii Kirp. Although the name G. tranzschelii have been treated as synonym of G. uliginosum by several authors, its distinct morphology might be sufficient to separate to two independent taxa. Generally, the morphological polymorphisms and hybridization of G. uliginosum complicate the taxonomy of the species, and thus further investigation for their habitat, distribution and morphology were needed to their taxonomic entity.

• ALGAE
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• 제27권2호
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• pp.109-114
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• 2012

Water motion is one of the main drivers in morphological variability in species within de order Laminariales, and most of our current knowledge is based on subtidal populations. $Eisenia$ $arborea$ is a dominant kelp species in the North Pacific, widely distributed along the Baja California Peninsula from mid intertidal down to subtidal areas. This species presents great variability in the intertidal zone but it has not been yet evaluated such variability according to wave exposure. The present work also identifies the spatial / temporal variation, particularly respect to the presence of stipes without medulla (hollow stipes) a feature common among other brown seaweeds. We evaluated the effects of wave action in morphological variation of intertidal $Eisenia$ $arborea$ (Laminariales, Ochrophyta) at Punta Eugenia. The spatial and temporal variation sampling was surveyed between February, May, July, and August 2004 in the intertidal of Punta Eugenia, Baja California Sur. Our results have shown that exposed sites correlate with increased length and width of stipes as compared to more protected sites. Hollow stipes frequency changed more in association with temporal variation than with spatial heterogeneity suggesting nutrient limitation for thalli development. Our results suggest that $Eisenia$ $arborea$ compensate by morphological modifications the stress of living in the intertidal zone by showing larger stipes. Hollow stipes might be are also a mechanical adaptation to increase survival in high energy environments.

• 대한전자공학회논문지SP
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• 제43권3호
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• pp.84-91
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• 2006

본 논문에서는 수학적 모폴로지 연산과 가우시안 혼합 모형에 기초한 새로운 칼라 영상 분할 알고리즘을 제안한다. 우리는 혼합 모형에서 구성 성분의 수를 결정하고, 각 구성 성분의 중심값을 계산하는데 모폴로지의 연산과 라벨링 연산을 이용한다. 그리고 칼라 특징 벡터의 확률 모형으로 가우시안 혼합 모형을 사용하고, 이들의 모수 값들을 추정하는데 결정적 어닐링 EM알고리즘을 사용한다. 최종적으로 혼합 모형으로부터 계산된 사후 확률을 이용하여 칼라 영상을 분할한다. 실험 결과를 통하여 모폴로지 연산이 혼합모형의 수를 자동으로 결정하고 각 성분의 모드를 계산하는데 아주 효율적인 방법임을 보였고, 또한 결정적 어닐링 EM 알고리즘에 의하여 추정된 가우시안 혼합 모형을 사용하여 계산된 사후 확률에 의한 영상 분할 방법이 기존의 분할 알고리즘보다 정확한 분할 방법임을 보였다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1146-1151
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• 2005

The yeast Candida albicans has a distinguishing feature, dimorphism, which is the ability to switch between two morphological forms: a budding yeast form and a multicellular invasive filamentous form. This ability has been postulated to contribute to the virulence of this organism. Previously, we reported that the yeast-to-hypha transition in this organism is suppressed by farnesoic acid, a morphogenic autoregulatory substance that accumulates in the medium as the cells proliferate. In this study, using a differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique, we have identified several genes induced in C. albicans by farnesoic acid treatment. These observations indicate that farnesoic acid can alter the expressivity of multiple genes, including the DNA replication machinery and cell-cycle-control proteins.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.736-739
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• 2006

While hyperspectral data are very rich in information, their processing poses several challenges such as computational requirements, noise removal and relevant information extraction. In this paper, the application of advanced scale-space filtering to selected hyperspectral bands was investigated. In particular, a pre-processing tool, consisting of anisotropic diffusion and morphological leveling filtering, has been developed, aiming to an edge-preserving smoothing and simplification of hyperspectral data, procedures which are of fundamental importance during feature extraction and object detection. Two scale space parameters define the extent of image smoothing (anisotropic diffusion iterations) and image simplification (scale of morphological levelings). Experimental results demonstrated the effectiveness of the developed scale space filtering for the enhancement and smoothing of hyperspectral remote sensing data and their advantage against watershed over-segmentation problems and edge detection.