• Archives of Pharmacal Research
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• 제11권3호
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• pp.213-217
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• 1988

The effects of ethanol and acetaldehyde on monoamine oxidase activity in mouse liver mitochondrial fraction were studied. In vivo, a single dose of ethanol increased the hepatic monoamine oxidase activity compared to control group, and chronic ethanol consumption also increased the enzyme activity using tyramine, benzylamine or serotonin as substrate. Acetaldehyde, the metabolite of ethanol, significantly increased monoamine oxidase activity more than ethanol did. In contrast to the in vivo results, it was found that the monoamine oxidase activity was inhibited in vitro by ethanol or acetaldehyde in the dose-dependent manner.

• 대한약리학회지
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• 제8권2호
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• pp.41-47
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• 1972

Fluorides were supposed to exert a stimulatory action on the catecholamine release. In this study, the authors attempted to investigate the action of sodium fluoride on the catecholamine release from the isolated perfused cow adrenal gland and rat heart. And also the inhibitory effect of sodium fluoride on the monoamine oxidase activity in rat heart and liver mitochondria was investigated. The monoamine oxidase activity was measured by the conversion of benzylamine to benzaldehyde. The results obtained were follows; 1. Sodium fluoride stimulated the release of catecholamine from the isolated perfused cow adrenal gland and rat heart. 2. Sodium fluoride inhibited the rat heart and liver mitochondrial monoamine oxidase activity.

• Archives of Pharmacal Research
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• 제13권2호
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• pp.132-135
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• 1990

Polyhydroxystilbenes including resveratrol were reported to competitively inhibit monoamine oxidase-A-without structural relation with substrates and cynthetic inhibitors for the enzyme. We attempt to explore a plausible mechanism for their inhibitory activity on MAO-A. All the polyhydroxystilbenes tested showed the antioxidant activity on liver homogenate. Furthermore, the antioxidant activity turned out to closely correlate with the MAO-A inhibitory activity.

• 한국식품영양학회지
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• 제17권4호
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• pp.347-355
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• 2004

초석잠 메탄올 추출물이 뇌신경전달물질과 관련이 있는 acetylcholine esterase, monoamine oxidase 및 xanthine oxidase의 활성억제효과에 대하여 실험하였다. 실험관내에서 초석잠 추출물을 각각 100 및 200mg/kg씩 첨가한 다음 실험한 결과 첨가농도가 증가할수록 과산화지질의 생성을 억제하여 용량 의존형으로 나타났다. 쥐를 대상으로 20일간 투여한 동물실험에서는 초석잠 추출물 100 mg/kg을 식이에 혼합하여 투여한 결과 지질 과산화의 생성을 억제하였다. Acetylcholine esterase의 활성의 경우 초석잠 추출물을 100 mg/kg 투여한 결과 대조군에 비하여 효소활성이 23.11%로 억제되었고, monoamine oxidase 및 xanthine oxidase 활성이 각각 21.93% 및 63.58%로 억제되었다. 그리고 초석잠 추출물을 100 mg/kg을 투여한 경우 xanthine dehydrogenase로부터 xanthine oxidase로의 형전환비율은 28%로 현저하게 억제되었다. 또한, Xanthine dehydrogenase로부터 oxidase로의 형전환비율을 실험한 결과 정상상태에 비하여 억제됨을 알 수 있었다.

• 생약학회지
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• 제29권4호
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• pp.271-276
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• 1998

The inhibitory effects of MeOH extracts of 100 medicinal herbs on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and its activity was determined by fluorospectrophotomer using kynuramine as a substrate. Nine kinds of MeOH extracts of herbs including Artemisia iwayomogi showed a mild inhibitory effect with ${100}-{200}\;{\mu}/ml$ in their $IC_{50}$ values on MAO activity. Seventeen MeOH extracts including Juglans mandshurica exhibited a weak inhibition of MAO activity with ${200}-{300}\;{\mu}/ml$ in their $IC_{50}$ values.

• 생약학회지
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• 제37권3호
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• pp.157-161
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• 2006

We examined the inhibitory activities against monoamine oxidase (MAO) of Prunella vulgaris in vitro and in vivo methods. Methanolic extract of P. vulgaris showed significantly Inhibitorγ activities on MAO-A and MAO-B that were prepared from rat brain and liver in vitro. The inhibitory activities were measured by serotonin and benzylamine as substrates, respectively. MAO-A and MAO-B activities were potently inhibited by ethylacetate extracts of P. vulgaris in vitro tests. It was observed that those activities in vivo tests have different tendency each other. MAO-A activity was increased by the oral administration of methanolic extract of P. vulgaris while MAO-B activity was decreased. Consequently, we suggest that P. vulgaris may have the effects on the inhibitory activities against MAO both in vitro and in vivo.

• 보험의학회지
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• 제1권1호
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• pp.84-87
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• 1984

1. 전신가토(全身家兎) 및 척수가토(脊髓家兎)에서 MAO 억제하(抑制下)에서의 brethylium의 tyramine 승압효과(昇壓效果)에 미치는 영향(影響)을 관찰(觀察)하였다. 2. 전신가토(全身家兎) 및 척수가토(脊髓家兎)에서 bretylium 정주(靜注) 후(後)에는 tyramine의 승압효과(昇壓效果)는 강화(强化)되었으나 MAO 억제제(抑制劑)인 catron을 정주(靜注)하고 bretylium을 추가(追加) 주입(注入)한 후(後)에는 tyramine의 승압효과(昇壓效果)는 catron 투여전(投與前)의 그것보다 감약(減弱)되었다. 3. Bretylium에 의한 tyramine 승압효과(昇壓效果)의 강화(强化)는 MAO 억제작용(抑制作用)으로 설명(說明)되고, tyramine 승압효과(昇壓效果)의 감약(減弱)은 교감신경말단(交感神經末端)의 차단작용(遮斷作用)으로 설명(說明)된다.

• 생약학회지
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• 제37권4호
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• pp.320-323
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• 2006

The effects of evodiamine on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and the $K_m\;and\;V_{max}$ values of MAO were $78.5{\pm}5.28{\mu}M$ and $0.68{\pm}0.07$ nmol/min/mg protein, respectively (n=4). Evodiamine at $30-120{\mu}M$ showed an inhibitory effect on MAO activity using a substrate kynuramine with an $IC_{50}$ value of $104.2{\mu}M$ (n=4). Evodiamine also exhibited a non-competitive inhibition on MAO. The $K_i$ value for evodiamine was $72.5{\pm}10.8{\mu}M$ (n=4). These results suggest that evodiamine partially contributes to the regulation of monoamine content.

• Applied Biological Chemistry
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• 제42권2호
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• pp.166-169
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• 1999

두충잎으로부터 얻은 MeOH 추출물의 n-BuOH 분획으로부터 silica gel column chromatography를 반복하여 monoamine oxidase B (MAO-B) 억제 활성을 갖는 flavonoid 배당체를 분리하였다. 화합물의 화학구조를 스펙트럼 데이터의 해석과 산가수분해반응을 이용하여 $3-O-[{\beta}-D-glucopyranosyl\;(1{\rightarrow}2)\;{\beta}-D-xylopyranosyl]\;quercetin$으로 결정하였다. 이 화합물의 MAO-B 억제활성에 있어서의 $IC_{50}$ 값은 $8.05\;{\mu}mol/l$로 측정되었다.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2428-2432
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• 2011

A series of new 4-aryl-6,8-dimethylpyrimido[4,5-c]pyridazine-5,7(6H,8H)-diones have been synthesized via three component reaction of 1,3-dimethylbarbituric acid with arylglyoxals in the presence of hydrazinium dihydrochloride in ethanol. All of these derivatives may act as potential monoamine oxidase inhibitors.