• 한국막학회:학술대회논문집
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• 한국막학회 2003년도 춘계 총회 및 학술발표회
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• pp.45-48
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• 2003

• 폴리머
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• 제37권3호
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• pp.288-293
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• 2013

A group of molecularly imprinted polymers (MIPs) with specific recognition for chiral (S)-ibuprofen were successfully prepared based on hydrogen bonds, utilizing ${\alpha}$-methacrylic acid as a functional monomer. The IR analysis of MIPs showed that the blue- and red-shifted hydrogen bonds were formed between templates and functional monomers in the process of self-assembly imprinting and re-recognition, respectively. According to UV-Vis analysis, we found that the ratio of host-guest complexes between template molecule and functional monomer was 1:1. The effect of cross-linker's quantity on the polymerization was studied by transmission electron microscope (TEM). The adsorption selectivity experiments indicated that MIPs exhibited higher selectivity to (S)-ibuprofen than those to ketoprofen and (R)-ibuprofen, (S)-ibuprofen's structural analogs.

• KSBB Journal
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• 제17권2호
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• pp.207-211
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• 2002

습식 상 전환법으로 theophylline이 각인된 poly(acryloni-trile-co-acrylic acid) 막을 제조하였다. theophylline은 고분자가 공중합되는 과정에 각인시키거나(in-situ implanting) 제자리 동시 각인) 공중합된 고분자를 DMSO 용액에 녹이면서 template를 섞어 각인시켰다(post implanting, 후 각인). 막의 template molecule에 대한 흡착 선택도는 응고시키는 물의 온도가 높을수록, 혼합물의 초기 농도가 높을수록 크게 나타났다. 후 각인법으로 제조된 막의 흡착선택도가 제자리 동시각인 제작법으로 제조된 막보다 흡착선택도가 우수하였다.

• 공업화학
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• 제19권3호
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• pp.287-294
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• 2008

본 연구에서는 서로 상반되는 생리 활성을 나타내는 거울상 이성질체를 분리하기 위해 UV 중합법에 의해 이성질체를 인식할 수 있는 분자 인식형 고분자(molecularly imprinted polymers; MIPs) 분리막 제조와 그 분리막을 이용한 이성질체의 분리 선택성에 관한 연구를 수행하였다. 폴리카보네이트(polycarbonate; PC) 막은 기공(pore) 내벽에 작은 spot의 형태로 중합되었고 양성(anodisc; AD) 막은 기공 내벽이 아닌 표면(surface) 부분에 500~700 nm 정도의 필름(film) 형태로 중합되었다. 한편, 제조된 MIPs 분리막들의 L-트립토판(tryptophane; Trp) 이성질체 용액을 이용한 분리 선택도 비교 결과, 기능성 단량체인 메타아크릴산(methacrylic acid; MAA)에 대하여 가교제인 에틸렌 글리콜 디메타아킬레이트(ethylene glycol dimethacrylate; EGDMA) 90% 이상, 분자제인 메탄올(methanol; MeOH)이 30% 이하 첨가되어 필름 형태로 중합된 AD MIPs 분리막이 3.5의 우수한 선택도를 가졌다.

• KSBB Journal
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• 제16권2호
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• pp.115-122
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• 2001

Molecular imprinting has now been established as a technique which allows the creation of tailor-made binding sites for many classes of compounds. MIPs were prepared by covalent and non-covalent chemical bonding systems, by interactions between functional monomer and template. The shape of MIP is divided to particle and membrane. MIP membranes can be prepared by surface imprinting, in-situ polymerization, wet phase inversion and the dry phase inversion method. MIPs have been mainly used for analytical separation and biosensor systems to separate and detect chiral compounds and materials with similar structures. However the application of MIP by the chemical industries is still in its infancy stages. This review summarizes the preparative characteristics and applications of MIP with respect to chiral separations and biosensors.

• 멤브레인
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• 제31권3호
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• pp.219-227
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• 2021

분자각인막(MIM)은 특정 분자의 결합자리를 갖고 있는 다공성 고분자 막이다. 비용매유도 상분리(NIPS)법을 사용하여 분자각인막의 지지체로 사용될 P(AN-co-MA) 비대칭막을 제조하고, 여기에 표면 각인법을 적용하여 광활성 이니퍼터 도입과 photo-grafting을 통해 라이소자임 분자각인막을 제조하였다. P(AN-co-MA) 비대칭막을 3-chloropropyltrimethoxysilane과 광활성 이니퍼터 dithiocarbamate로 개질하고, acrylamide 단량체, N,N'-methylenebisacrylamide 가교제, 라이소자임 주형분자 혼합액을 UV 조사 환경에서 혼성중합 시켜 MIM을 제조하였다. 제조된 MIM의 FT-IR과 FE-SEM 및 EDS 분석을 실시한 결과 P(AN-co-MA) 막은 비대칭 단면 구조이었고 표면에 이니퍼터 그룹이 잘 결합되어 있어 MIM이 성공적으로 제조되었다. 제조공정의 변수 조절을 통해 라이소자임의 흡착량이 비각인막(NIM)에 비해 13배 큰 값인 2.7 mg/g을 갖는 P(AN-co-MA) 기반 MIM이 제조되었으며, 막여과 실험을 통해 라이소자임에 대한 오발부민의 투과선택도를 측정한 결과 MIM은 라이소자임의 선택적 결합력이 우수하였다.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3348-3354
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• 2011

Oleanolic acid (OA) as template molecule, polyamide-6 (PA6) as basement membrane and poly(styrene-comaleic acid) (PSMA) were used to prepare PA6/PSMA-OA molecularly imprinted composite membranes by phase inversion method in supercritical $CO_2$ ($ScCO_2$). The template molecule (OA), [poly(styrene-co-maleic anhydride) (PSMAH), PSMA, molecularly imprinted membranes (MIMs) imprinting OA and MIMs after elution were all characterized by Fourier transform infrared spectroscopy (FTIR). The conditions that were the mass ratio between PSMA and OA from 3:1 to 8:1, temperature of $ScCO_2$ from $35^{\circ}C$ to $50^{\circ}C$ and pressure of $ScCO_2$ 12 MPa to 17 MPa were studied. It was obtained the largest adsorption rate and purity of OA after adsorption of the resultant MIMs, 50.41% and 96.15% respectively. After using PA6 film and non-woven fabrics as basement membrane respectively, it was found that smaller aperture of PA6 was used as basement membrane, a higher adsorption rate and a higher purity of OA after adsorption of the MIMs were obtained, and so were the stability and reproducibility of the resultant MIMs. After template molecules being removed, the MIMs had effective selectivity hydrogen bonding to separately bind in the binary components to the template molecules-oleanolic acid.

• Journal of Ginseng Research
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• 제46권5호
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• pp.621-627
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• 2022

Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb₁ from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb₁ from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl₂ to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides.