Browse > Article
http://dx.doi.org/10.14579/MEMBRANE_JOURNAL.2021.31.3.219

Preparation and Characteristics of P(AN-co-MA) Membrane Imprinted with Lysozyme Molecules  

Min, Kyoung Won (Department of Engineering Chemistry, Chungbuk National University)
Yoo, Anna (Department of Engineering Chemistry, Chungbuk National University)
Youm, Kyung Ho (Department of Engineering Chemistry, Chungbuk National University)
Publication Information
Membrane Journal / v.31, no.3, 2021 , pp. 219-227 More about this Journal
Abstract
Molecularly imprinted membrane (MIM) is a porous polymer membrane incorporating with the molecular recognizing sites. In this study, the supporting P(AN-co-MA) asymmetric membrane was prepared by nonsolvent induced phase separation (NIPS) method. And then, MIM with lysozyme template sites was prepared using the surface imprinting method on the P(AN-co-MA) asymmetric membrane introducing a photoactive iniferter and then photo-grafting. The P(AN-co-MA) asymmetric membrane was modified with 3-chloropropyltrimethoxysilane and dithiocarbamate as a photoactive iniferter. To prepare a lysozyme imprinted membrane, the modified P(AN-co-MA) membrane was copolymerized with acrylamide as a functional momomer, N,N'-methylene bisacrylamide as a crosslinker and lysozyme as a template in the UV irradiation environment. The lysozyme imprinted MIM was analyzed by using SEM, FT-IR and EDS measurements. Its results confirm that all the P(AN-co-MA) membranes have an asymmetric structure and the iniferter group is successfully introduced on the membrane surface. The process parameters were adjusted to obtain MIM having the excellent lysozyme adsorption. The maximum lysozyme adsorption capacity reaches at 2.7 mg/g, which is 13 times higher than that of the non imprinted membrane (NIM). The permselective membrane filtration experiments of ovalbumin to lysozyme show that the P(AN-co-MA) MIM preferentially bounds a greater amount of lysozyme.
Keywords
Molecularly Imprinted membrane (MIM); P(AN-co-MA) membrane; Lysozyme; Photoactive Ininiferter; Permselectivity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 T. H. Choi, and H. B. Park, "Membrane and virus filter trends in the processes of biopharmaceutical production", Membr. J., 30, 9 (2020).   DOI
2 J. Lee, J. Lee, and Y. Baek, "Ultrafiltration and diafiltration processes for concentration and formulation of antibody-based therapeutics", Membr. J., 30, 373 (2020).   DOI
3 I. Park, S. Y. Yoo, and H. B. Park, "How to design membrane chromatography for bioseparations: A short review", Membr. J., 31, 145 (2021).   DOI
4 K. H. Youm, H. Y. Lee, and Y. C. Shin, "Water treatment using separation membranes", pp. 25-27, Sin-A Publishing, Jeju, Korea (2011).
5 M. Yoshikawa, K. Tharpa, and S. Dima, "Molecularly Imprinted Membranes: Past, Present, and Future", Chem. Rev., 116, 11500 (2016).   DOI
6 S. A. Piletsky, T. L. Panasyuk, E. V. Piletskaya, I. A. Nicholls, and M. Ulbricht, "Receptor and transport properties of imprinted polymer membranes - A review", J. Memb. Sci., 157, 263 (1999).   DOI
7 H. R. Park, and K. H. Youm, "Separation of lysozyme from chicken egg white by ultrafiltration (1) - Dead-end filtration of egg white solution", Membr. J., 26, 266 (2016).   DOI
8 E. Y. Lee, and G. J. Woo, "Separation of highly purified antimicrobial lysozyme using ultrafiltration and characteristics of membrane fouling", Korean J. Food Sci. Technol., 31, 458 (1999).
9 H. Y. Lee, and B. S. Kim, "Grafting of molecularly imprinted polymers on iniferter-modified carbon nanotube", Biosens. Bioelectron., 25, 587 (2009).   DOI
10 M. C. Prete, D. M. Dos Santos, L. Effting, and C. R. T. Tarley, "Preparation of molecularly imprinted poly(methacrylic acid) grafted on iniferter-modified multiwalled carbon nanotubes by living-radical polymerization for 17β-estradiol extraction", J. Chem. Eng. Data, 64, 1978 (2019).   DOI
11 S. J. Dong, "Preparation and characterization of molecular imprinting polymer membranes based on P(AN-co-MA) and cellulose", Master's Thesis, Chungbuk National University, Chungbuk, Korea (2014).
12 A. L. Zydney, "New developments in membranes for bioprocessing - A review", J. Membr. Sci., 620, 118804 (2021).   DOI
13 P. Fan, and B. Wang, "Preparation of molecularly imprinted polymer membrane with blending trimethoprim-MIP and polysulfone and its transport properties", Korean J. Chem. Eng., 26, 1813 (2009).   DOI
14 R. Ghosh, and Z. F. Cui, "Purification of lysozyme using ultrafiltration", Biotech.. Bioeng., 68, 191 (2000).   DOI
15 Y. Matsuoka, "Egg white lysozyme", J. Japan Soc. Food Nat., 24, 311 (1971).