• Bulletin of the Korean Chemical Society
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• 제25권5호
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• pp.609-616
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• 2004

Complexation of cesium ion by p-tert-butylcalix[6]arene ethyl ester was studied by NMR spectroscopy in nonpolar $CDCl_3$ and polar acetone-$d_6$ and the results were compared with each other. Analysis of temperature dependent $^1H$ spectra and titration curves reveals that both solvents result in a 1 : 1 cone-form complex with nonpolar $CDCl_3$yielding a more tightly bound one than acetone-$d_6$. Unexpectedly, at very low temperature, we have found that two phenyl ring proton peaks of equal intensity appear both in $CDCl_3$and in acetone-$d_6$ solution which gradually collapse and eventually coalesce into a single line as temperature is raised. This observation could be interpreted in terms of the chemical exchange through direct and/or indirect interconversion between two equivalent conformations possible the complex in both solvents over the temperature range observed. And broadening of $^{133}Cs$ (I = 7/2) nmr line with increasing temperature has also been observed, indicating the exchange of $^{133}Cs$ ion between the complex and the solvent. From numerical fitting of lineshape changes for one-dimensional $^1H$ and $^{133}Cs$ spectra, the exchange rate constants and other relevant parameters for this conformational interconversion and the complex-solvent exchange were deduced.

• BMB Reports
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• 제43권1호
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• pp.62-68
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• 2010

Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-${\kappa}B$ signal pathway.

• 한국전기전자재료학회논문지
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• 제11권9호
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• pp.687-692
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• 1998

Silicon doped $Al_{0.32}Ga_{0.68}As$ were growth by molecular beam epitaxy. Electroreflectance(ER) spectra of the $E_1$ transition of Schottky barrier Au/n-$Al_{0.32}Ga_{0.68}As$ have been measured at various modulation voltage($V_{ac}$) and dc bias voltage($V_{bias}$). from the $E_1$peak, band gap energy of the $Al_{0.32}Ga_{0.68}As$ is 1.883 eV which corresponds to an Al composition of 32%. As modulation voltage($V_{bias}$) is changed, a line shape at the $E_1$transition does not change, but its amplitude varies linearly. The amplitude of $E_1$signal decrease with increasing the forward dc bias voltage($V_{bias}$), but the line shape does not change. It suggests that the low field theory rather than Franz-Keldysh oscillation is Required to interpret spectra. Also, spectra at the $E_1$transition were broadened with increasing the reverse dc bias voltage($V_{bias}$) which suggests the presence of Field-induced broadening.

• 천문학논총
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• 제30권2호
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• pp.99-101
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• 2015

Massive stars are some of the most influential objects in the Universe, shaping the evolution of galaxies, creating chemical elements and hence shaping the evolution of the Universe. However, the processes by which they form and how they shape their environment during their birth processes are not well understood. We use $NH_3$ data from "The $H_2O$ Southern Galactic Plane Survey" (HOPS) survey to define the positions of dense cores/clumps of gas in the southern Galactic plane that are likely to form stars. Then, using data from "The Millimetre Astronomy Legacy Team 90 GHz" (MALT90) survey, we search for the presence of infall and outflow associated with these cores. We subsequently use the "3D Molecular Line Radiative Transfer Code" (MOLLIE) to constrain properties of the infall and outflow, such as velocity and mass flow. The aim of the project is to determine how common infall and outflow are in star forming cores, and therefore to provide valuable constraints on the timescales and physical process involved in massive star formation. Preliminary results are presented here.

• 천문학회보
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• 제44권1호
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• pp.69.1-69.1
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• 2019

We present the ALMA observations of the infrared dark cloud (IRDC) core MSXDC G053.11+00.05 MM1 at the distance of 1.7 kpc. While the core was first identified at 1.2 mm with a mass of 124 Msun, recent near- and mid-infrared observations have revealed a parsec-scale molecular hydrogen (H2 1-0 S(1) at 2.12 micron) outflow and two early class young stellar objects (YSOs) at the center of the core, one of which is likely massive (M > 8 Msun). From the ALMA Band 7 observations with a resolution of 0.5", we have found a dust filament of < 0.1 pc in which five dense cores are embedded in the 870 micron continuum. The brightest core is consistent with one of the two previously-detected YSOs, but the other four are newly discovered implying their very deeply embedded status. We have also detected several molecular line emission including H13CO+ and C17O as well as 13CO outflow with complicated morphology. At the brightest core, the methanol line (CH3OH) shows velocity gradients, which may support the existence of a circumstellar disk around a high-mass protostar. Based on the derived properties of the dense cores, we discuss their association with the two YSOs and H2 outflow detected in infrared and high-mass star-formation process occurring in IRDC cores.

• 대한방사성의약품학회지
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• 제7권2호
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• pp.69-77
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• 2021

No-carrier-added holmium-166 (n.c.a ¹⁶⁶Ho) separation is performed based on the results of separation conditions using stable isotopes dysprosium (Dy) and holmium (Ho) to minimize radioactive waste from separation optimization procedures. Successful separation of two adjacent lanthanides was achieved by cation-exchange chromatography using a sulfonated resin in the H+ form (BP-800) and α-hydroxyisobutyric acid (α-HIBA) as eluent. For the identification process after separation of stable isotopes, the use of chromogenic reagents alternatively enables on-line detection because the lanthanides are hardly absorb light in the UV-vis region or exhibit radioactivity. Four different chromogenic reagents were pre-tested to evaluate suitable coloring reagents, of which 4-(2-Pyridylazo)resorcinol is the most recommendable considering the sensitivity and specificity for lanthanides. Lanthanide radioisotopes (RI) were monitored for separation with an RI detector using a lab-made separation LC system. Under the proper separation conditions, the n.c.a ¹⁶⁶Ho was effectively obtained from a large amount of 100 mg dysprosium target within 2 hrs.

• 천문학회지
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• 제36권4호
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• pp.271-282
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• 2003

We present results of a $H^{13}CN$ J=1-0 mapping survey of molecular clouds toward the Galactic Center (GC) region of $-1.6^{\circ}{\le}{\iota}{\le}2^{\circ}$ and $-0.23^{\circ}{\le}b{\le}0.30^{\circ}$ with 2' grid resolution. The $H^{13}CN$ emissions show similar distribution and velocity structures to those of the $H^{12}CN$ emissions, but are found to better trace the feature saturated with $H^{12}CN$ (1-0). The bright components among multi-components of $H^{12}CN$ line profiles usually appear in the $H^{13}CN$ line while most of the dynamically forbidden, weak $H^{12}CN$ components are seldom detected in the $H^{13}CN$ line. We also present results of other complementary observations in $^{12}CO$ (J=1-0) and $^{13}CO$ (J=1-0) lines to estimate physical quantities of the GC clouds, such as fractional abundance of HCN isotopes and mass of the GC cloud complexes. We confirm that the GC has very rich chemistry. The overall fractional abundance of $H^{12}CN$ and $H^{13}CN$ relative to $H_2$ in the GC region is found to be significantly higher than those of any other regions, such as star forming region and dark cloud. Especially cloud complexes nearer to the GC tend to have various higher abundance of HCN. Total mass of the HCN molecular clouds within $[{\iota}]{\le}6^{\circ}$ is estimated to be ${\~}2 {\times}10^7\;M_{\bigodot}$ using the abundances of HCN isotopes, which is fairly consistent with previous other estimates. Masses of four main complexes in the GC range from a few $10^5$ to ${\~}10^7\;M_{\bigodot}$ All the HCN spectra with multi-components for the four main cloud complexes were investigated to compare the line widths of the complexes. The largest mode (45 km $s^{-1}$) of the FWHM distributions among the complexes is in the Clump 2. The value of the mode tends to be smaller at the farther complexes from the GC.

• BMB Reports
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• 제49권2호
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• pp.99-104
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• 2016

The nuclear non-histone protein high mobility group box (HMGB) 1 is known to having an inhibitory effect on the repair of DNA damaged by the antitumor drug cisplatin in vitro. To investigate the role of HMGB1 in living cells, we studied the DNA repair of cisplatin damages in mouse fibroblast cell line, NIH-3T3. We evaluated the effect of the post-synthetic acetylation and C-terminal domain of the protein by overexpression of the parental and mutant GFP fused forms of HMGB1. The results revealed that HMGB1 had also an inhibitory effect on the repair of cisplatin damaged DNA in vivo. The silencing of HMGB1 in NIH-3T3 cells increased the cellular DNA repair potential. The increased levels of repair synthesis could be "rescued" and returned to less than normal levels if the knockdown cells were transfected with plasmids encoding HMGB1 and HMGB1 K2A. In this case, the truncated form of HMGB1 also exhibited a slight inhibitory effect.

• 천문학논총
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• 제27권4호
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• pp.261-262
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• 2012

The aim of this research is to reveal the spatial distribution of the star formation activity of nearby galaxies by comparing CO molecular emission lines with the large area observation in far-infrared (FIR) lines. We report the imaging observations of NGC 253 by FIR forbidden lines via FIS-FTS and CO molecular lines from low to high excitation levels with ASTE, which are good tracers of star forming regions or photo-dissociation regions, especially spiral galaxies, in order to derive the information of the physical conditions of the ambient interstellar radiation fields. The combination of spatially resolved FIR and sub-mm data leads to the star formation efficiency within galaxy. The ratio between the FIR luminosity and molecular gas mass, $L_{FIR}/M_{H_2}$, is expected to be proportional to the number of stars formed in the galaxy per unit molecular gas mass and time. Moreover the FIR line ux shows current star formation activity directly. Furthermore these can be systematic and statistical data for star formation history and evolution of spiral galaxies.

• Molecules and Cells
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• 제27권1호
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• pp.5-14
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• 2009

The availability of effective vaccines has had the most profound positive effect on improving the quality of public health by preventing infectious diseases. Despite many successful vaccines, there are still old and new emerging pathogens against which there is no vaccine available. A better understanding of how vaccines work for providing protection will help to improve current vaccines as well as to develop effective vaccines against pathogens for which we do not have a proper means to control. Recent studies have focused on innate immunity as the first line of host defense and its role in inducing adaptive immunity; such studies have been an intense area of research, which will reveal the immunological mechanisms how vaccines work for protection. Toll-like receptors (TLRs), a family of receptors for pathogen-associated molecular patterns on cells of the innate immune system, play a critical role in detecting and responding to microbial infections. Importantly, the innate immune system modulates the quantity and quality of long-term T and B cell memory and protective immune responses to pathogens. Limited studies suggest that vaccines which mimic natural infection and/or the structure of pathogens seem to be effective in inducing long-term protective immunity. A better understanding of the similarities and differences of the molecular and cellular events in host responses to vaccination and pathogen infection would enable the rationale for design of novel preventive measures against many challenging pathogens.