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http://dx.doi.org/10.5483/BMBRep.2010.43.1.062

Inhibition of the expression on MMP-2, 9 and morphological changes via human fibrosarcoma cell line by 6,6'-bieckol from marine alga Ecklonia cava  

Zhang, Chen (Department of Chemistry, Pukyong National University)
Li, Yong (Marine Bioprocess Research Center, Pukyong National University)
Shi, Xiujuan (Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University)
Kim, Se-Kwon (Department of Chemistry, Pukyong National University)
Publication Information
BMB Reports / v.43, no.1, 2010 , pp. 62-68 More about this Journal
Abstract
Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-${\kappa}B$ signal pathway.
Keywords
Activator protein-1 (AP-1); Ecklonia cava (EC); Matrix metalloproteinases (MMPs); Nuclear factor-kappa B (NF-${\kappa}B$); 6,6'-bieckol;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
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