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Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study (지르코니아 표면에 부착된 바이오필름에 대한 LED 치솔의 항균효과)

  • Park, Jong Hew;Kim, Yong-Gun;Um, Heung-Sik;Lee, Si Young;Lee, Jae-Kwan;Chang, Beom-Seok
    • Journal of Dental Rehabilitation and Applied Science
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    • v.35 no.3
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    • pp.160-169
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    • 2019
  • Purpose: The purpose of this study was to evaluate the antimicrobial effects of a toothbrush with light-emitting diodes (LEDs) on periodontitis-associated dental biofilm attached to a zirconia surface by static and dynamic methods. Materials and Methods: Zirconia disks (12 mm diameter, 2.5 mm thickness) were inserted into a 24-well plate (static method) or inside a Center for Disease Control and Prevention (CDC) biofilm reactor (dynamic method) to form dental biofilms using Streptococcus gordonii and Fusobacterium nucleatum. The disks with biofilm were subdivided into five treatment groups-control, commercial photodynamic therapy (PDT), toothbrush alone (B), brush with LED (BL), and brush with LED+erythrosine (BLE). After treatment, the disks were agitated to detach the bacteria, and the resulting solutions were spread directly on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy (SEM) was performed to visualize alterations in bacterial morphology. Results: No significant difference in biofilm formation was observed between dynamic and static methods. A significant difference was observed in the number of viable bacteria between the control and all experimental groups (P < 0.05). The percentage of bacterial reduction in the BLE group was significantly higher than in the other treated groups (P < 0.05). SEM revealed damaged bacterial cell walls in the PDT, BL, and BLE groups, but intact cell walls in the control and B groups. Conclusion: The findings suggest that an LED toothbrush with erythrosine is more effective than other treatments in reducing the viability of periodontitis-associated bacteria attached to zirconia in vitro.

Effect of LED Irradiation on Growth Characteristids of Ginseng Cultivated in Plastic Film House

  • Seo, Sang Young;Cho, Jong hyeon;Kim, Chang Su;Kim, Hyo Jin;Kim, Dong Won;An, Min Sil;Yoon, Du Hyeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.45-45
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    • 2019
  • This experiment was carried out using artificial clay and LED in the plastic film house (irradiation time: 08:00~18:00/day). Seedlings (n = 63 per $3.3m^2$) of ginseng was planted on May 17, 2018. LED was combined with red and blue light in a 3:1 ratio and irradiated with different light intensity. The average air temperature from April to September was $12.3^{\circ}C$ $-26.0^{\circ}C$ and it was the the highest at $26.0^{\circ}C$ in August. The test area where fluorescent lamp was irradiated tended to be somewhat higher than the LED irradiation area. The chemical properties of the test soil are as follows. pH levels was 5.3~5.5, EC levels 0.45~0.52 dS/m and OM levels 33~37%. The total nitrogen content was 0.35~0.47% and the available $P_2O_5$ contents was 13.7~16.0 mg/kg, which was lower than the suitable level of 70~200 mg/kg. Exchangeable cations K and Mg contents were within acceptable ranges, but the Ca contents was $28{\sim}38cmol^+/kg$ levels higher than the permissible level ($2{\sim}6cmol^+/kg$). Germination of ginseng leaves took 8~9 days and the overall germination rate was 70~75%. The photometric characteristics of LED light intensity are as follows. The greater the light intensity, the higher the PAR (Photosynthetic Action Radiation) value, illuminance and solar irradiation. Photosynthetic rate was also increased with higher light intensity was investigated at $1.7{\sim}3.2{\mu}mol\;CO_2/m^2/s$. Leaf temperature ($23.7{\sim}24.8^{\circ}C$) by light intensity was the same trend. The growth of aerial parts (plant height etc.) were generally excellent when irradiated with 3 times the light intensity, the growth of the ginseng aerial parts were excellent as follows. The plant height was 42.6 cm, stem length was 25.2 cm, leaf length was 9.6 cm and stem diameter was 5.0 mm. The growth of underground part (root length etc.) was the same, and the root length was 24.4 cm, the tap root length was 6.0 cm, diameter of taproot was 18.2 mm and the fresh root weight was 17.2 g. There were no disease incidence such as Alternaria blight, Gray mold and Anthracnose. Disease of Damping off occurred 2.2~3.6% and incidence ratio of rusty root ginseng was 14.6~20.7%. Leaf discoloration rate was 13.7~48.9% and increased with increasing light intensity. Ginsenoside content of ginseng by light intensity is under analysis.

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EFFECT OF LIGHT SOURCE AND SHADE ON DEPTH OF CURE OF COMPOSITES (중합광원과 레진 색상이 복합레진의 중합깊이에 미치는 영향)

  • Na, Joon-Sok;Jeong, Sun-Wa;Hwang, Yun-Chan;Kim, Sun-Ho;Yun, Chang;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.27 no.6
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    • pp.561-568
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    • 2002
  • Purpose of this research is estimating polymerization depth of different source of light. XL 3000 for halo-gen light, Apollo 95E for plasma arc light and Easy cure for LED light source were used in this study. Different shade (B1 & A3) resin composites (Esthet-X, Dentsply, U.S.A.) were used to measure depth of cure. 1, 2, and 3 mm thick samples were light cured for three seconds, six seconds or 10 seconds with Apollo 95E and they were light cured with XL-3000 and Easy cure for 10 seconds, 20 seconds, or 40 seconds. Vicker's hardness test carried out after store samples for 24 hours in distilled water. Results were as following. 1. Curing time increases from al1 source of lights, oui$.$ing depth increased(p<0.05). 2. Depth (that except 1mm group and 2mm group which lighten to halogen source of light) deepens in all groups, Vickers hardness decreased(p<0.05). 3. Vicker's hardness of A3 shade composite was lower in all depths more than B1 shade composites in group that do polymerization for 10 seconds and 20 seconds using halogen source of light(p<0.05), but group that do polymerization lot 40 seconds did not show difference(p>0.05). 4. Groups that do polymerization using Plasma arc and LED source of light did not show Vicker's hardness difference according to color at surface and 1mm depth(p>0.05), but showed difference according to color at 2mm and 3mm depth(p<0.05). The results showed that Apollo 95E need more polymerization times than manufacturer's recommendation (3 seconds), and Easy cure need polymerization time of XL-3000 at least.

The Effect of Squeezing Parameters on the Fabrication Behavior of Phosphor Films (스퀴징 공정변수에 따른 형광체막 성형 거동에 관한 연구)

  • Park, J.Y.;Lee, J.W.;Yoon, G.S.
    • Transactions of Materials Processing
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    • v.24 no.2
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    • pp.95-100
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    • 2015
  • It was confirmed that when phosphor slurry is formed in the cavity of an elastic mold, the pressure distribution of the phosphor slurry varies as a function of the major squeegee parameters (squeegee angle, squeegee velocity, and the viscosity of the phosphor slurry). The higher the slurry viscosity, the faster the squeegee velocity, and the smaller the squeegee angle, the higher the filling completeness of the phosphor slurry. The optimum conditions for complete filling of the phosphor slurry were found when the squeegee angle was between 30 to 45 degrees, squeegee velocity at 40 to 70mm/sec, and the viscosity of the phosphor slurry composite was at 6,556 cps (i.e. phosphor content around 50 wt. %).

EFFECT OF SOFT-START LIGHT CURING ON THE POLYMERIZATION AND THE CONTRACTION STRESS OF COMPOSITE RESIN (완속기시(Soft-start) 광조사 방식이 복합레진의 중합 및 수축응력에 미치는 효과)

  • Wee, You-Min;Oh, You-Hyang;Lee, Nan-Young;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.2
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    • pp.332-343
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    • 2005
  • The purpose of this study was to evaluate the influence of soft-start light curing on contraction stress and hardness of composite resin. Composite resin mold was cured using the one-step continuous curing method with three difference light sources; conventional halogen light curing for 40 seconds at $400\;mw/cm^2$, plasma arc light curing for 6 seconds at $1300\;mW/cm^2$ and LED light curing for 10 seconds at $7The purpose of this study was to evaluate the influence of soft-start light curing on contraction stress and hardness of composite resin. Composite resin mold was cured using the one-step continuous curing method with three difference light sources; conventional halogen light curing for 40 seconds at . For the soft-start curing method ; 2 seconds light exposure at $650\;mW/cm^2$ followed by 3 seconds at $1300\;mW/cm^2$ and exponential increase with 5 seconds followed by 10 seconds at $700\;mW/cm^2$ were used. Contraction stress was measured using strain gauge method and Vickers hardness was measured 24 hours after polymerization at the top and bottom of specimens. Resin-acrylic interfaces were observed using a scanning electron microscope(SEM). The results of present study can be summarized as follows: 1. Contraction stresses at 10 min after polymerization were significantly reduced with the soft-start curing both in plasma and LED light sources(P<0.05). 2. Plasma light curing with soft-start resulted in not only the lowest contraction stress, but also the lowest hardness(P<0.05) 3. LED light curing with soft-start showed lower contraction stress than the one-step continuous halogen and LED light curing(P<0.05). 4. Microhardness of specimens cured by LED light with soft-start was equivalent to that of cured by the one-step continuous halogen and LED light(P>0.05). 5. Curing by LED light with soft-start and conventional halogen light resulted in better marginal sealing than plasma light and one-step LED light curing.

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A COMPUTER ANALYSIS ON THE KOREAN CONSONANT SOUND DISTORTION IN RELATION TO THE PALATAL PLATE THICKNESS -Dentoalveolar and hard palatal consonant- (구개상의 두께에 따른 한국어 자음의 발음 변화에 관한 컴퓨터 분석 - 치조음, 경구개음-)

  • Woo, Yi-Hyung;Choi, Dae-Kyun;Choi, Boo-Byung;Park, Nam-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.25 no.1
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    • pp.71-94
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    • 1987
  • This study was carried out to investigate the sound distortion following the alternation of the palatal plate thickness. For this study, 2 healthy male subjects (24-year-old) were selected. Born in Seoul, they both spoke Seoul dialect. First, their sounds of /na(나)/, /da(다)/, /1a(라)/, /ja(자)/, /cha(차)/, /ta(타)/, without inserting plates were recorded, and then the sounds with palatal plates of different thickness were recorded, successively. The plate was fabricated in 3 types, each palatal thickness being 1.0mm, 2.5mm, dentoalveolar portion 2.5mm, other residual portion was 1.0mm, successively. Each type plates named B, C, D-type, in succession. Series of analysis were administered through Computer(16 bit) to analyze the sound distortions. These experiments were analyzed by the LPC (without weighting, pre-weighting, post-weighting) of the consonants, vowels portion, formant frequency of the vowels and word duration of the consonants. The findings led to the following conclusions: 1. There was no correlation of the distortion rate on the 2 informants. 2. Generally, vowels were not affected by the palatal plate thickness in the formant analysis, however, more distortion was detected in the LPC analysis, especially C, D-type plates. 3. Consonants distortion was more evident in the C, D-type plate. 4. The second formant was most disturbed and reduced in the all consonants with insertion of the palatal plate, especially C, D-type plate. 5. Word duration was shortened in the plate inserted(except /ja/, /cha/), especially C, D-type. 6. It was found that dentoalveolar, hard palatal sounds were severely distorted in plate inserted, and they were mainly affected by the dentoalveolar portion thickness. 7. There was correlation between palatal thickness and consonants quality.

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AN ACOUSTIC STUDY IN RELATION TO THE SOUND DISTORTION BY THE ALTERATION OF PALATAL PLATE -FOCUSSED ON/ㅅ(s)/. BY COMPUTER ANALYSIS- (구개상의 형태 변화가 발음에 미치는 영향에 관한 음향학적 연구 -/ㅅ/을 중심으로한 컴퓨터 분석-)

  • Choi, Chang-Kyu;Woo, Y.H.;Park, Nam-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.27 no.1
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    • pp.83-102
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    • 1989
  • This study was done to analyze the sound distortion, before and after insertion of the palatal palates. For this study, 4 healthy subjects (3 males and 1 female, each 24-year-old), who were born in Seoul were recruited from K university, and 3 type palatal plates were fabricated, each palatal thickness being 1.0mm, 2.5mm, dentoalveolar portion 2.5mm and elsewhere 1.0mm, named B,C,D-type repectively, and informants's sounds of /사(sa), 서(se), 소(so), 수(su), 스($s\.{+}$), 시(si)/ were recorded, without plate, and with palatal plates of different types, in succession. A series of analysis were adminstered through a 16 Bit IBM PC/AT using linear combination methods. These experiments were analyzed by the Cepstrum (Weighted and Euclidian), Log Area Ratio, Linear prediction correlation methods The findings led to the following conclusions : 1. It was confirmed that the same consonant, /ㅅ(s)/, variously distorted by the following vowel. 2. By and large, 시($s\.{+}$) was the most distorted in all conditions, and (sa), 소(so) were the least distorted in each condition. 3. There were no persistant correlation of the palatal plate types, and sound distortions of each informant were diverse with no regularities. 4. There were persistent correaltion to the Cepstrum (Weighted, Euclidian), Log Area Ratio. However, Linear prediction correlation has a different alteration pattern.

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Effect of Epidermal Growth Factor with Collagen Matrix on Increasing Gingival Thickness: A Pilot Preclinical Investigation

  • Hyun-Chang Lim;Yeek Herr;Jong-Hyuk Chung;Seung-Yun Shin;Seung-Il Shin;Ji-Youn Hong
    • Journal of Korean Dental Science
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    • v.16 no.2
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    • pp.172-181
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    • 2023
  • Purpose: To investigate the effect of epidermal growth factor (EGF) with collagen matrix (CM) for increasing gingival thickness. Materials and Methods: In five mongrel dogs, bilateral gingival defects were surgically made on the maxillary canines. After two months, either a subepithelial connective tissue graft (group SCTG) or CM with EGF (0.1 ug/ml, group EGF) was grafted, and the flap was coronally positioned to cover the graft materials. The animals were sacrificed after three months. Intraoral scanning was performed for soft tissue analysis. Histologic and histomorphometric analyses were performed. Result: Two animals exhibited wound dehiscence during the healing phase, leaving three for analysis. No statistically significant difference was found in soft tissue changes (P>0.05). The level of gingival margin (GM) increased in both groups (1.02±0.74 mm in group SCTG vs. 1.24±0.83 mm in group EGF). Linear increases at the GM pre-augmentation in the soft tissue profile were 1.08±0.58 mm in group SCTG and 0.96±0.73 mm in group EGF. Histomorphometric parameters (keratinized tissue height, tissue thickness, and rete peg density) were not significantly different between the groups (P>0.05). Conclusion: EGF loaded onto CM led to comparable gingival phenotype enhancement to SCTG.

New Light Fiber BLU System for Large LCD Display

  • Chung, Man-Young;Park, Tong-Soo
    • Proceedings of the IEEK Conference
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    • 2002.07c
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    • pp.1398-1400
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    • 2002
  • A new PLF-BLU (Plastic Light Fiber-Back Light Unit) using side glowing light fiber rods array for the 42" LCD TV display has been evelopedd. The light fibers were 14mm in diameter and 50cm long pure transparent acrylic rods of 1.49 refractive index. Fine seratees were made on the flat side of rod. extremely bright incandscent light from lamp fed into the fiber si scattered at scratches then emerges through the surface of rod. A typical PLF-BLU system consists of 24 PLFs produced side glow of brightness of 4,500cd/㎡ to 6,500cd/㎡. New PLF-BLU is proved to be a BLU of rigid, bright, no heat generation, and low power consumption, hence a prospective BLU system for very and/or ultra large size TVs. A new LED-PLF-BLU system considered to be a revolutionary to break-through of the BLU technologies has also been developed, and is decribed briefly.

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THERMAL CHANGE AND MICROHARDNESS IN CURING COMPOSITE RESIN ACCORDING TO VARIOUS CURING LIGHT SYSTEM (광중합기에 따른 복합레진 중합시 온도 변화와 미세경도에 관한 연구)

  • Lee, Dong-Jin;Kim, Dae-Eop;Yang, Yong-Sook;Lee, Kwang-Hee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.3
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    • pp.391-399
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    • 2004
  • The purpose of this study was to compare curing efficiency of newly developed curing units to traditional halogen curing unit by measuring thermal change and surface microhardness according to curing light system. Materials and mathods : The types of curing units were traditional low intensity halogen light(Optilux 360), plasma arc light(Flipo), low heat plasma arc light(Aurys), low intensity LED(Starlight), and high intensity LED(Freelight2). Temperature at the tip of light guide was measured by a digital thermometer using K-type thermocouple. And after resin was filled to 2, 3, 4mm teflon mold, bottom temperature measured during curing. After 24 hours, microhardness of top surface and bottom surface of each resin specimen were measured. Results : The result of this study can be summarized as follows, 1. As measuring temperature of curing unit tips, Flipo is the highest as $52.4^{\circ}C,\;Freelight2(37.86^{\circ}C),\;Optilux360(32.68^{\circ}C),\;Aurys(32.34^{\circ}C),\;and\;Starlight(26.14^{\circ}C)$ were followed. 2. Flipo and Freelight2 were the highest similarly and Optilux360 and Aurys were similarly next and Starlight was lowest in temperature of bottom surface of resin mold. 3. Microhardness of top surface were generally similar, and Aurys was relatively low. 4. Optilux 360 and Freelight2 were the highest, and Flipo, Starlight, and Aurys were followed in microhardness of bottom surface. Conclusions : The results suggest that careful use of Flipo and Freelight2 might be able to cure greater depth of resin composite and do not cause thermal problems than other curing units.

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