Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Effect of Epidermal Growth Factor with Collagen Matrix on Increasing Gingival Thickness: A Pilot Preclinical Investigation

  • Hyun-Chang Lim (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center) ;
  • Yeek Herr (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center) ;
  • Jong-Hyuk Chung (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center) ;
  • Seung-Yun Shin (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center) ;
  • Seung-Il Shin (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center) ;
  • Ji-Youn Hong (Department of Periodontology, Kyung Hee University College of Dentistry, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center)
  • Received : 2023.06.14
  • Accepted : 2023.08.02
  • Published : 2023.12.30
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Abstract

Purpose: To investigate the effect of epidermal growth factor (EGF) with collagen matrix (CM) for increasing gingival thickness. Materials and Methods: In five mongrel dogs, bilateral gingival defects were surgically made on the maxillary canines. After two months, either a subepithelial connective tissue graft (group SCTG) or CM with EGF (0.1 ug/ml, group EGF) was grafted, and the flap was coronally positioned to cover the graft materials. The animals were sacrificed after three months. Intraoral scanning was performed for soft tissue analysis. Histologic and histomorphometric analyses were performed. Result: Two animals exhibited wound dehiscence during the healing phase, leaving three for analysis. No statistically significant difference was found in soft tissue changes (P>0.05). The level of gingival margin (GM) increased in both groups (1.02±0.74 mm in group SCTG vs. 1.24±0.83 mm in group EGF). Linear increases at the GM pre-augmentation in the soft tissue profile were 1.08±0.58 mm in group SCTG and 0.96±0.73 mm in group EGF. Histomorphometric parameters (keratinized tissue height, tissue thickness, and rete peg density) were not significantly different between the groups (P>0.05). Conclusion: EGF loaded onto CM led to comparable gingival phenotype enhancement to SCTG.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2020R1C1C1008201). Epidermal growth factor (EGF) was kindly provided by Daewoong, Seoul, Korea. Collagen matrices were kindly provided by Genoss, Seoul, Korea.

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