• 제목/요약/키워드: minimum growth inhibition

검색결과 126건 처리시간 0.024초

대황목단탕의 메티실린 내성 황색 포도상구균에 대한 항균활성 (The Antimicrobial Activity of Daehwangmokdan-tang against Methicillin-resistant Staphylococcus aureus)

  • 한형선;권동렬;강옥화
    • 대한본초학회지
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    • 제35권3호
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    • pp.47-54
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    • 2020
  • Objective : Infectious diseases are a growing problem worldwide by Methicillin-resistant Staphylococcus aureus (MRSA). Daehwangmokdan-tang is one of the oriental medicine prescriptions contained in Principles and Practice of Eastern Medicine. This study investigated the antibacterial activity of EtOH 70% extracts of Daehwangmokdan-tang (DMT) which prescription is composed of oriental medicine against MRSA. Methods : The antimicrobial activity and active concentration of MRSA were verified by measuring the minimum inhibitory concentration (MIC) of DMT. In addition, the effects of the disease were checked by treating the existing antibiotics and large ethanol extract in parallel, and the extent of growth suppression was checked over time. In addition, cell membrane permeability experiment confirmed the effect of large DMT on the immunity mechanism of MRSA. Results : TThe minimum inhibitory concentration of DMT against MRSA is 500 ~ 2000 ㎍/㎖ by broth dilution method. In the checkerboard method, the combinations of DMT with antibiotics has partial synergistic effect or synergy effect and DMT markedly reduced the MICs of the antibiotics oxacillin (OX), gentamicin (GEN) against MRSA. In the inhibition of resistance mechanism of DMT against MRSA, the expression of resistance gene and protein about β-lactam antibiotic was reduced. Also, we observed the effect of DMT about cell membrane permeability against MRSA, and confirmed that DMT suppressed growth of strains by increasing cell membrane permeability and energy metabolism. Conclusion : Basis on the result, we speculate that DMT may be useful for the treatment of MRSA infections when used in combination with β-lactam antibiotic.

지유 에탄올 추출물의 식품부패균에 대한 항균효과 (Antimicrobial Effects Against Food-borne Pathogens of Sanguisorbae Officinalis L. Ethanol Extract)

  • 최무영;임태진
    • 한국지역사회생활과학회지
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    • 제24권1호
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    • pp.27-36
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    • 2013
  • This study was performed to investigate the antimicrobial effect against food-borne pathogens of Sanguisorbae officinalis L. ethanol extract. The antimicrobial activity of the ethanol extract was determined using a paper disc-diffusion method and the diameter of the clear zone was measured. The diameters of the clear zone in the presence of 10 mg of the ethanol extract were the maximum against Staphylococcus aureus among the tested 4 gram-positive bacteria and Pseudomonas aeruginosa among the tested 7 gram-negative bacteria. Analysis of the minimum inhibition concentrations (MIC) showed that the ethanol extract exhibited a similar efficacy as sorbic acid, well-known chemical preservatives. The growth inhibitory effects of the ethanol extract in the concentrations of 250, 500, 1,000 and 2,000 mg/L on food-borne pathogens were determined against Staphylococcus aureus, Bacillus subtilis, Salmonella Typhimurium and Pseudomonas aeruginosa. The growth of the microorganisms was significantly (p<0.05) inhibited by the ethanol extract in the concentrations higher than 250 mg/L. Thus, the results of the present study demonstrate that the ethanol extract exhibits antimicrobial effects against food-borne pathogens, suggesting that Sanguisorbae officinalis L. could be used as natural antibacterial agent in food.

미나리발효액이 장내 유해세균 및 유익균의 In Vitro 생육 및 효소활성에 미치는 영향 (Effect of Extract of Fermented Dropwort on Intestinal Bacteria and Enzymes In Vitro)

  • 이경애;김무성;조홍범
    • 미생물학회지
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    • 제44권4호
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    • pp.358-361
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    • 2008
  • 미나리(Oenanthe stolonifera) 발효액이 장내 병원성 미생물과 유익균의 생육, 그리고 장내 세균효소에 미치는 영향을 in vitro 에서 조사하였다. 고상 배지 (Agar plate) 에서의 clear zone 형성에 의한 생육저해를 측정한 결과, Vibro, Salmonella 등의 유해 미생물에 대해서 강한 생육저해 효과를 보였으며 Bifidobacterium longum 에 대해서는 생육저해 효과가 크지 않았다. 액체배지에서의 최소저해농도(MIC) 측정에서도 고장배지에서와 같은 경향을 보여 상대적으로 B. longum 에 대한 생육저해가 가장 적었다. 발효 기간에 따른 영향을 보면 발효 기간이 길수록 적은 양으로도 유해한 균들의 생육을 잘 저해할 수 있는 것으로 나타났다. 장내 세균 효소인 ${\beta}$-glucuronidase와 tryptophanase의 활성에 대해 미나리 발효액은 발효하지 않은 액에 비해 저해효과가 컸으며 발효기간이 길수록 저해효과도 증가하였다. 이상의 실험 결과로서 미나리 발효액은 유해세균에 대한 생육저해능이 크고 상대적으로 유익균인 B. longum에 대한 저해는 적어, 음용 시 정장 효과가 있을 것으로 추정된다.

섬바디로부터 L. monocytogenes에 대한 생장억제 물질의 분리 및 구조동정 (Isolation and Identification of Growth Inhibition Substance on L. monocytogenes from Dystaenia takesimana Kitagawa)

  • 오진아;신동화;백남인
    • 한국식품과학회지
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    • 제31권4호
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    • pp.984-993
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    • 1999
  • 섬바디 75% 에탄올 추출물과 핵산 분획물을 첨가하여 Listeria monocytogenes(Lm) ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 및 ATCC 15313에 대한 증식억제 효과와 그 활성 물질의 구조를 확인하였다. 항균효과를 시험한 결과 에탄올 추출물은 50 ppm, 핵산 분획물의 경우에는 30 ppm에서 5종의 Listeria 균주를 모두 증식저해 시켰다. 항균활성이 우수한 섬바디 핵산 분획물을 silica gel column으로 1회 분획하여 획분 A8을 얻었고 이의 항균효과는 대단히 우수하여 10 ppm에서 실험균주 모두를 증식저해 시켰다. A8이 아주 우수한 항균효과를 나타내었기에 살균효과가 기대되었다. Tryptic soy broth에 섬바디 추출물에서 분리된 A8를 30 및 10 ppm씩 배지에 첨가한 후 Lm 5균주를 배양시켜 24시간 간격으로 생균수를 확인한 결과 대조구는 균수가 증가하는 반면 처리구는 균수가 감소함으로서 살균효과가 인정되었다. 그리고 이 획분 A8을 2회 연속 분리하여 단리된 소획분 SBD-1과 SBD-2를 얻었다. 이렇게 단일 물질로 분리된 SBD-1과 SBD-2의 항균효과는 column 분리후 얻은 획분(A8)보다 증식억제 효과가 감소하였는데 이는 섬바디에 존재하는 항균물질은 단독으로 작용하는 것이 아니라 그 안에 있는 여러 물질들의 혼합작용으로 그 효과가 나타난 것이라 추정되었다. 단일물질로 분리된 두 물질 중 수율이 높은 SBD-2를 구조 동정한 결과 폴리아세틸렌 화합물 (9z)-hepta-deca-1,9-dien-4,6-diyn-3,8-diol 인 falcarindiol로 확인되었다. 이 물질은 미나리과 식물 뿌리에 존재한다.

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국화 '백마'의 겨울철 재배시 야간최저온도가 생장과 개화에 미치는 영향 (Effect of Night Minimum Temperature During Winter Season on Growth and Flowerng in Chrysanthemum 'Baekma')

  • 최성열;허은주;권오근;임진희
    • 화훼연구
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    • 제17권4호
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    • pp.226-230
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    • 2009
  • '백마'의 겨울철 재배시 야간최저온도가 생장 및 생리장해 발생에 미치는 영향을 알아보기 위하여 야간최저 온도를 각각 $14^{\circ}C$, $16^{\circ}C$, $18^{\circ}C$로 처리한 후 온도별 생육 및 개회특성, 생리장해 발생 등을 '신마'와 비교하여 조사하였다. 발뢰소요일수는 모든 처리 온도에서 '백마'가 '신마'보다 2-11일 정도 길었으나, 개회소요일수는 두 품종 모두 54-59일 정도로 품종간에 유의차가 없었고, 야간 최저온도가 높아짐에 띠라 개화소요일수가 짧아지는 경향을 보였다. 절화장 및 절회중은 두 품종 모두 야간최저온도가 높아질수록 감소하였고, 꽃잎수는 '백마'의 경우 $16^{\circ}C$에서 300개 이었으나, $14^{\circ}C$$18^{\circ}C$에서는 각각 210개, 233개로 적어져 온도에 따라 큰 차이가 있었지만, '신마'의 경우에는 온도간 차이가 없었다. 동계 저온에 의한 생리장해 중 로젯트는 '백마'에서만 발생하였으며 $14^{\circ}C$에서 11.1%, $16^{\circ}C$에서 55.6%, $18^{\circ}C$에서 56.1% 발생하였다. 단일처리 후 미발뢰율은 야간최저온도 $14^{\circ}C$에서 '백마' 17.3%, '신마' 4% 이었다.

비듬 증상 완화를 위한 에션셜 오일의 유효성 연구 - 티트리 오일을 중심으로 - (A Study on Efficiency of Essential Oil for Dandruff Symptoms Relief - Focused on Tea tree oil -)

  • 박은하
    • 한국패션뷰티학회지
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    • 제3권3호
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    • pp.31-40
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    • 2005
  • The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

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Cephalosporium acremonium M-113의 세팔로스포린에 의한 생장억제 효과 (Inhibitory Effect of Cephalosporin C on Growth of Cephalosporium acremonium M-113)

  • Kim, Myung-Kuk;Park, Sang-Ho;Lee, Jeong-Kug;Kho, Yung-Hee;Mheen, Tae-Ick
    • 한국미생물·생명공학회지
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    • 제13권4호
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    • pp.339-344
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    • 1985
  • 항세균성 항생제인 세팔로스포린 C가 그 자신의 생산균주인 C. acremonium M-113의 생장을 저해하였다. 비슷한 저해현상이 C. acremonium ATCC 20339와 ATCC 14553에도 관찰되었다. 세팔로스포린C의 최소 생육 저지농도가 분생포자의 경우 200-500$\mu\textrm{g}$/$m\ell$이었고 균사의 경우 3,000-4,000 $\mu\textrm{g}$/$m\ell$이었다. 이 최소 생육 저지농도는 복합배지에서 더욱 상승되었다. 세팔로스포린C는 배양초기에 존재할 경우. 그 생육 저지 효과를 나타내었다. 세팔로스포린 C의 생육 저지 효과는 세팔로스포린 C가 아미노산의 세포 내로의 수송을 방해함으로써 나타나는 것으로 조사되었다. 생육 저지 기저에 세팔로스포린 C의 3' 기가 중요 역할을 하는 것으로 나타났다. 또한, 세팔로스포린 C는 배지에 질소원이 결핍될 때 질소원으로 이용될 수 있는 것으로 나타났다.

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황금(Scutellaria baicalensis) 추출물에 의한 Streptococcus mutans의 항균 및 부착억제 효과 (Effects of Antibacteria and Adhesive Inhibition of Scutellaria baicalensis Extract on Streptococcus mutans)

  • 백종윤;김용현;권현정;김은님;김완종;한만덕
    • 치위생과학회지
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    • 제8권4호
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    • pp.367-373
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    • 2008
  • 천연물 황금으로부터 여러 유기용매 추출물을 얻어 치아우식증 원인균인 S. mutans 에 대한 항균활성, 배지 내 환경변화, 그리고 부착억제에 대해 알아보았다. 1. 구강 질병인 치아우식증의 대표적 원인균 S. mutans에 대한 황금 ethyl acetate 추출물 (IPK-3)의 최소억제농도는 125 mg/ml 이었다. 2. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때 균의 생장 량과 최대 생장시간은 대조군보다 지연되었다. 3. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때, pH 변화는 대조군은 18시간에서 pH 5.63으로 급격한 변화를 보였으며 황금 IPK-3 추출물이 50 mg/ml의 배지에서는 6.50이상을 유지하여 pH가 변화가 없었다. 4. 황금 IPK-3 추출물을 첨가한 배지 내 탄수화물, 단백질 및 균체 외 다당류의 변화는 대조군에 비해 매우 낮게 생산되었다. 5. 균체를 형광염료(DAPI)로 염색하여 S-HA 부착억제을 확인한 결과, 대조군은 시간이 경과함에 따라 S.mutans의 hydroxyapatite(HA)에 부착 정도가 증가되었으나, 황금 IPK-3 추출물이 첨가된 시험군에서는 S-HA에 균의 부착이 매우 적었다. 이같이 황금의 IPK-3 (ethyl acetate 추출물)이 치아우식증의 원인균인 S. mutans 에 대한 항균활성효과 뿐만아니라 hydroxyapatite에 부착 억제 효과도 있었다.

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식물체의 연(Pb) 흡수 및 유해성에 미치는 음이온의 영향 (Effects of Various Anions on Absorption and Toxicity of Lead in Plants)

  • 성민웅
    • Journal of Plant Biology
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    • 제20권1호
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    • pp.7-14
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    • 1977
  • The seeds of bean(Glycine max M.) and corn(Zea mays L.) soaked in 5000ppm lead solution for 24 hours were sowed in the flowerpots being placed sandy-clay soil under the field condition. The fixed concentrations of various anions and 2000 ppm lead were supplied alternately in the sandy-clay soi of the flowerpots at two days interval from May to July in 1976. After the plants were harvested prior to the flowering stage, the lead contents of plnat and soil were analyzed by atomic absorption spectrophotometer. The lead contents absorbed by the plant roots showed the highest in an weak acid soil of the best suitable condition of plant growth. The absorption of lead by the plant roots was inhibited by the various anions, especially divalent anions of the soil. Some phosphate anions inhibited lead absorption more than otehr various anions in the soil. The more various anions were in the soil, the more plants could be protected from the lead toxicity. In the case of lead supply in the soil, 99.5% of lead was accumulated in the upper layer of the soil(0-10cm), and 0.5% of lead accumulated in the lower layer (10-20cm). Therefore, the yellow-brown and white symptoms on the leaves and the inhibition of root growth by lead toxicity was increased in the early stage of the germination, however decreased in accordance with the progress of the growing stage becuase of the root growth toward beneath the lower layer of the soil. In spite of the contents of 3773ppm lead in the soil, the symptoms of lead toxicity was not found in the grown plants. At that time the lead contents of the plants absorbed from the soil were minimum 0.78ppm and maximum 3.64ppm through the growing stage.

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Biocidal Activity of Metal Nanoparticles Synthesized by Fusarium solani against Multidrug-Resistant Bacteria and Mycotoxigenic Fungi

  • Sayed, Manal T. El;El-Sayed, Ashraf S.A.
    • Journal of Microbiology and Biotechnology
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    • 제30권2호
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    • pp.226-236
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    • 2020
  • Antibiotic resistance by pathogenic bacteria and fungi is one of the most serious global public health problems in the 21st century, directly affecting human health and lifestyle. Pseudomonas aeruginosa and Staphylococcus aureus with strong resistance to the common antibiotics have been isolated from Intensive Care Unit patients at Zagazig Hospital. Thus, in this study we assessed the biocidal activity of nanoparticles of silver, copper and zinc synthesized by Fusarium solani KJ 623702 against these multidrug resistant-bacteria. The synthesized Metal Nano-particles (MNPs) were characterized by UV-Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and Zeta potential. The Fourier transform infrared spectroscopy (FTIR) result showed the presence of different functional groups such as carboxyl, amino and thiol, ester and peptide bonds in addition to glycosidic bonds that might stabilize the dispersity of MNPs from aggregation. The antimicrobial potential of MNPs by F. solani against the multidrug-resistant (MDR) P. aeruginosa and S. aureus in addition to the mycotoxigenic Aspergillus awamori, A. fumigatus and F. oxysporum was investigated, based on the visual growth by diameter of inhibition zone. Among the synthesized MNPs, the spherical AgNPs (13.70 nm) displayed significant effect against P. aeruginosa (Zone of Inhibition 22.4 mm and Minimum Inhibitory Concentration 21.33 ㎍/ml), while ZINC oxide Nano-Particles were the most effective against F. oxysporum (ZOI, 18.5 mm and MIC 24.7 ㎍/ml). Transmission Electron Microscope micrographs of AgNP-treated P. aeruginosa showed cracks and pits in the cell wall, with internalization of NPs. Production of pyocyanin pigment was significantly inhibited by AgNPs in a concentration-dependent manner, and at 5-20 ㎍ of AgNPs/ml, the pigment production was reduced by about 15-100%, respectively.