The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Antimicrobial Activity of Daehwangmokdan-tang against Methicillin-resistant Staphylococcus aureus

대황목단탕의 메티실린 내성 황색 포도상구균에 대한 항균활성

  • Han, Hyoung-Sun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute)
  • 한형선 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소)
  • Received : 2020.04.09
  • Accepted : 2020.05.25
  • Published : 2020.05.30
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Abstract

Objective : Infectious diseases are a growing problem worldwide by Methicillin-resistant Staphylococcus aureus (MRSA). Daehwangmokdan-tang is one of the oriental medicine prescriptions contained in Principles and Practice of Eastern Medicine. This study investigated the antibacterial activity of EtOH 70% extracts of Daehwangmokdan-tang (DMT) which prescription is composed of oriental medicine against MRSA. Methods : The antimicrobial activity and active concentration of MRSA were verified by measuring the minimum inhibitory concentration (MIC) of DMT. In addition, the effects of the disease were checked by treating the existing antibiotics and large ethanol extract in parallel, and the extent of growth suppression was checked over time. In addition, cell membrane permeability experiment confirmed the effect of large DMT on the immunity mechanism of MRSA. Results : TThe minimum inhibitory concentration of DMT against MRSA is 500 ~ 2000 ㎍/㎖ by broth dilution method. In the checkerboard method, the combinations of DMT with antibiotics has partial synergistic effect or synergy effect and DMT markedly reduced the MICs of the antibiotics oxacillin (OX), gentamicin (GEN) against MRSA. In the inhibition of resistance mechanism of DMT against MRSA, the expression of resistance gene and protein about β-lactam antibiotic was reduced. Also, we observed the effect of DMT about cell membrane permeability against MRSA, and confirmed that DMT suppressed growth of strains by increasing cell membrane permeability and energy metabolism. Conclusion : Basis on the result, we speculate that DMT may be useful for the treatment of MRSA infections when used in combination with β-lactam antibiotic.

Keywords

References

  1. Livornese LL Jr, Dias S, Samel C, Romanowski B, Taylor S, May P, Pitsakis P, Woods G, Kaye D, Levison ME, Johnson CC. Hospital-acquired Infection with Vancomycin-resistant Enterococcus faecium Transmitted by Electronic Thermometers. Ann Intern Med. 1992; 15;117(2):112-6. https://doi.org/10.7326/0003-4819-117-2-112
  2. Holmes NE, Turnidge JD, Munckhof WJ, Robinson JO, Korman TM, O'Sullivan MV, Anderson TL, Roberts SA, Warren SJ, Johnson PD. Antimicrob Agents Chemother. Vancomycin AUC/MIC ratio and 30-day mortality in patients with Staphylococcus aureus bacteremia. Epub Jan 18. 2013; 57(4): 1654-63. https://doi.org/10.1128/AAC.01485-12
  3. Kock R, Becker K, Cookson B, van Gemert-Pijnen JE, Harbarth S, Kluytmans J, Mielke M, Peters G, Skov RL, Struelens MJ, Tacconelli E, Navarro Torne A, Witte W, Friedrich AW. Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Euro Surveill. 2010; 14;15(41):1968.
  4. Pence MA, Haste NM, Meharena HS, Olson J, Gallo RL, Nizet V, Kristian SA. Beta-Lactamase Repressor BlaI Modulates Staphylococcus aureus Cathelicidin Antimicrobial Peptide Resistance and Virulence. PLoS One. 2015; 25;10(8):e0136605. https://doi.org/10.1371/journal.pone.0136605
  5. Kim KJ, Yu HH, Cha JD, Seo SJ, Choi NY, You YO. Antibacterial activity of Curcuma longa L. against methicillin-resistant Staphylococcus aureus. Phytother Res. 2005;19(7):599-604. https://doi.org/10.1002/ptr.1660
  6. Saad A, Fadli M, Bouaziz M, Benharref A, Mezrioui NE, Hassani L. Anticandidal activity of the essential oils of Thymus maroccanus and Thymus broussonetii and their synergism with amphotericin B and fluconazol. Phytomedicine. 2010;17(13):1057-60. https://doi.org/10.1016/j.phymed.2010.03.020
  7. Ettefagh KA, Burns JT, Junio HA, Kaatz GW, Cech NB. Goldenseal (Hydrastis canadensis L.) extracts synergistically enhance the antibacterial activity of berberine via efflux pump inhibition. Planta Med. 2011;77(8): 835-40. https://doi.org/10.1055/s-0030-1250606
  8. Yoon JI, Bajpai VK, Kang SC. Synergistic effect of nisin and cone essential oil of Metasequoia glyptostroboides Miki ex Hu against Listeria monocytogenes in milk samples. Food Chem Toxicol. 2011;49(1):109-14. https://doi.org/10.1016/j.fct.2010.10.004
  9. Taleghani A, Emami SA, Tayarani-Najaran Z. Artemisia: a promising plant for the treatment of cancer. Bioorg Med Chem. 2020; 1;28(1):115180. https://doi.org/10.1016/j.bmc.2019.115180
  10. Chen JF. Synopsis of the Golden Chamber. 1st. Beijing: People's Medical Publishing House, 2000; 626-31.
  11. kim DH, Yoon M, Shin SS. Analysis for Compatibility of Daehwangmokdan-tang and Its Pharmacological Activities. Herbal Formula Science 2018; 26;(1): 81-102. https://doi.org/10.14374/HFS.2018.26.1.81
  12. Zhou L, Li D, Wang J, Liu Y, Wu J. Antibacterial phenolic compounds from the spines of Gleditsia sinensis Lam. Nat Prod Res. 2007; 21(4):283-91. https://doi.org/10.1080/14786410701192637
  13. Xian ZW. Modern research and application of Chengqi Decoction. 1st. Beijing: People's Medical Publishing House. 2011; 333-5.
  14. Duan F, Xin G, Niu H, Huang W. Chlorinated emodin as a natural antibacterial agent against drug-resistant bacteria through dual influence on bacterial cell membranes and DNA. Sci Rep. 2017; 5;7(1):12721. https://doi.org/10.1038/s41598-017-12905-3
  15. Gong Y, Lee DY, Lee JW, Choi DJ, Kim GS, Lee SH, Lee YS. Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus. Asian Pacific Journal of Tropical medicine. 2019; 12(1): 14-8. https://doi.org/10.4103/1995-7645.250339
  16. Lee JH, Kim YG, Ryu SY, Lee JT. Calciumchelating alizarin and other anthraquinones inhibit biofilm formation and the hemolytic activity of Staphylococcus aureus. Sci Rep. 2016; 14;6:19267. doi: 10.1038/srep19267.
  17. Gonzalez DJ, Vuong L, Gonzalez IS, Keller N, McGrosso D, Hwang JH, Hung J, Zinkernagel A, Dixon JE, Dorrestein PC, Nizet V. Phenol soluble modulin variants of community-associated methicillin-resistant Staphylococcus aureus captured using mass spectrometry-based molecular networking. 2014; 13(5):1262-72. https://doi.org/10.1074/mcp.M113.031336
  18. Lee YS, Kang OH, Choi JG, Oh YC, Keum JH, Kim SB, Jeong GS, Kim YC, Shin DW, Kwon DY. Synergistic effect of emodin in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. Pharm Biol. 2010; 48(11):1285-90. https://doi.org/10.3109/13880201003770150