• 한국항만경제학회지
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• 제31권2호
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• pp.23-40
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• 2015

본 연구는 시스템 다이내믹스 기법을 활용하여 주요 해운정책이 우리나라 외항선대 증가에 미치는 영향에 대하여 분석하는 것을 목적으로 하였다. 시뮬레이션을 위해 사용된 변수는 2005년부터 2013년까지 우리나라 해운기업이 보유한 선박척수와 총톤수, BDI, HRCI, CCFI, WS, 달러 대비 환율, 세계 선대규모, 우리나라 해운업계 부채비율 등이고, 해운정책이 외항선대 증가에 어떤 영향을 주는지를 검증하기 위하여 국제선박등록 톤수, 정책금융기관의 해운기업에 대한 대출액수, 톤세제 적용기업의 수, 선박펀드 규모 등 4가지 정책변수에 대해 민감도 분석을 실시하였다. 예측값의 정확도를 파악하기 위해 절대평균 오차비율(MAPE) 검증을 실시하였는데, MAPE 값이 3.46%로서 10%이내에 해당하여 매우 높은 정확도를 가지는 시뮬레이션 모델로 평가되었다. 민감도 분석결과, 국제선박등록 톤수, 정책금융기관의 대출액수, 선박펀드 규모, 톤세제 적용기업의 수의 순으로 영향도가 큰 것으로 분석되었다.

• Journal of Korean Neurosurgical Society
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• 제62권2호
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• pp.183-192
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• 2019

Objective : The objective of this study was to analyze patient-specific blood flow in ruptured aneurysms using obtained non-Newtonian viscosity and to observe associated hemodynamic features and morphological effects. Methods : Five patients with acute subarachnoid hemorrhage caused by ruptured posterior communicating artery aneurysms were included in the study. Patients' blood samples were measured immediately after enrollment. Computational fluid dynamics (CFD) was conducted to evaluate viscosity distributions and wall shear stress (WSS) distributions using a patient-specific geometric model and shear-thinning viscosity properties. Results : Substantial viscosity change was found at the dome of the aneurysms studied when applying non-Newtonian blood viscosity measured at peak-systole and end-diastole. The maximal WSS of the non-Newtonian model on an aneurysm at peak-systole was approximately 16% lower compared to Newtonian fluid, and most of the hemodynamic features of Newtonian flow at the aneurysms were higher, except for minimal WSS value. However, the differences between the Newtonian and non-Newtonian flow were not statistically significant. Rupture point of an aneurysm showed low WSS regardless of Newtonian or non-Newtonian CFD analyses. Conclusion : By using measured non-Newtonian viscosity and geometry on patient-specific CFD analysis, morphologic differences in hemodynamic features, such as changes in whole blood viscosity and WSS, were observed. Therefore, measured non-Newtonian viscosity might be possibly useful to obtain patient-specific hemodynamic and morphologic result.

• 한국컴퓨터그래픽스학회논문지
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• 제25권3호
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• pp.75-83
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• 2019

본 논문에서는 동일한 정점의 수와 연결 정보를 갖는 N개의 삼각 메쉬의 형상을 동시에 단순화하는 새로운 기법을 제시한다. 기존의 단순화 기법을 N개의 삼각 메쉬에 각각 적용하면, 정점의 개수는 같지만 삼각형을 구성하는 연결 정보가 서로 다른 단순화 메쉬를 생성한다. 이러한 한계는 고해상도 블렌드쉐입(blend shape) 모델에서 단순화된 블렌드쉐입 모델을 구성하는 것을 어렵게 한다. 본 논문에서 제시된 기법은 N개 메쉬 형상을 동시에 고려하여 최소의 제거 비용을 갖는 에지를 선택하여 단순화를 수행한다. 따라서 단순화 결과로 생성된 N개의 단순화 메쉬는 동일한 정점의 수와 연결 정보를 유지하게 된다. 다양한 얼굴 표정을 갖는 고해상도 블렌드쉐입 모델에 동시 단순화 기법을 적용하여 단순화된 블렌드쉐입 모델을 생성함으로써 제안된 기법의 효율성과 유효성을 입증한다.

• 농업과학연구
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• 제46권2호
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• pp.381-393
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• 2019

A smart farm is a system that combines information and communication technology (ICT), internet of things (IoT), and agricultural technology that enable a farm to operate with minimal labor and to automatically control of a greenhouse environment. Machine learning based on recently data-driven techniques has emerged with big data technologies and high-performance computing to create opportunities to quantify data intensive processes in agricultural operational environments. This paper presents research on the application of machine learning technology to diagnose the growth status of crops and predicting the harvest time of strawberries in a greenhouse according to image processing techniques. To classify the growth stages of the strawberries, we used object inference and detection with machine learning model based on deep learning neural networks and TensorFlow. The classification accuracy was compared based on the training data volume and training epoch. As a result, it was able to classify with an accuracy of over 90% with 200 training images and 8,000 training steps. The detection and classification of the strawberry maturities could be identified with an accuracy of over 90% at the mature and over mature stages of the strawberries. Concurrently, the experimental results are promising, and they show that this approach can be applied to develop a machine learning model for predicting the strawberry harvesting time and can be used to provide key decision support information to both farmers and policy makers about optimal harvest times and harvest planning.

• Molecules and Cells
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• 제44권8호
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• pp.602-612
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• 2021

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

• Journal of Ginseng Research
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• 제45권1호
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• pp.98-107
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• 2021

Background: Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioactive metabolites of the fungi associated with Panax ginseng Meyer. Methods: An endophytic fungus, Penicillium sp. SNF123 was isolated from the root of P. ginseng, from which acremonidin E was purified. Acremonidin E was tested on melanin synthesis in the murine melanoma cell line B16F10, in the human melanoma cell line MNT-1, and in a pigmented 3D-human skin model, Melanoderm. Results: Acremonidin E reduced melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 cells with minimal cytotoxicity. qRT-PCR analysis demonstrated that acremonidin E downregulated melanogenic genes, including tyrosinase and tyrosinase-related protein 1 (TRP-1), while their enzymatic activities were unaffected. The antimelanogenic effects of acremonidin E were further confirmed in MNT-1 and a pigmented 3D human epidermal skin model, Melanoderm. Immunohistological examination of the Melanoderm further confirmed the regression of both melanin synthesis and melanocyte activation in the treated tissue. Conclusion: This study demonstrates that acremonidin E, a bioactive metabolite derived from a fungal endophyte of P. ginseng, can inhibit melanin synthesis by downregulating tyrosinase, illuminating the potential utility of microorganisms associated with P. ginseng for cosmetic ingredients.

• Structural Engineering and Mechanics
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• 제77권4호
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• pp.441-461
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• 2021

Reinforced concrete (RC) columns are crucial in building structures and they are of higher vulnerability to terrorist threat than any other structural elements. Thus it is of great interest and necessity to achieve a comprehensive understanding of the possible responses of RC columns when exposed to high intensive blast loads. The primary objective of this study is to derive analytical formulas to assess vulnerability of RC columns using an advanced numerical modelling approach. This investigation is necessary as the effect of blast loads would be minimal to the RC structure if the explosive charge is located at the safe standoff distance from the main columns in the building and therefore minimizes the chance of disastrous collapse of the RC columns. In the current research, finite element model is developed for RC columns using LS-DYNA program that includes a comprehensive discussion of the material models, element formulation, boundary condition and loading methods. Numerical model is validated to aid in the study of RC column testing against the explosion field test results. Residual capacity of RC column is selected as damage criteria. Intensive investigations using Arbitrary Lagrangian Eulerian (ALE) methodology are then implemented to evaluate the influence of scaled distance, column dimension, concrete and steel reinforcement properties and axial load index on the vulnerability of RC columns. The generated empirical formulae can be used by the designers to predict a damage degree of new column design when consider explosive loads. With an extensive knowledge on the vulnerability assessment of RC structures under blast explosion, advancement to the convention design of structural elements can be achieved to improve the column survivability, while reducing the lethality of explosive attack and in turn providing a safer environment for the public.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.46-55
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• 2022

Clostridioides difficile infection (CDI) is a significant cause of hospital-acquired and antibiotic-mediated intestinal diseases and is a growing global public health concern. Overuse of antibiotics and their effect on normal intestinal flora has increased the incidence and severity of infections. Thus, the development of new, effective, and safe treatment options is a high priority. Here, we report a new probiotic strain, Bacillus amyloliquefaciens (BA PMC-80), and its in vitro/in vivo anti-C. difficile effect as a prospective novel candidate for replacing conventional antibiotics. BA PMC-80 showed a significant anti-C. difficile effect in coculture assay, and its cell-free supernatant (CFS) also exhibited a considerable anti-C. difficile effect with an 89.06 ㎍/ml 50% minimal inhibitory concentration (MIC) in broth microdilution assay. The CFS was stable and equally functional under different pHs, heat, and proteinase treatments. It also exhibited a high sensitivity against current antibiotics and no toxicity in subchronic toxicity testing in hamsters. Finally, BA PMC-80 showed a moderate effect in a hamster CDI model with reduced infection severity and delayed death. However, further studies are required to optimize the treatment condition of the hamster CDI model for better efficacy and identify the antimicrobial compound produced by BA PMC-80.

• International Journal of Computer Science & Network Security
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• 제21권11호
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• pp.301-311
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• 2021

The technological advancements of the last two decades directed the era of the Internet of Things (IoT). IoT enables billions of devices to connect through the internet and share their information and resources on a global level. These devices can be anything, from smartphones to embedded sensors. The main purpose of IoT is to make devices capable of achieving the desired goal with minimal to no human intervention. Although it hascome as a social and economic blessing, it still brought forward many security risks. This paper focuses on providing a survey of the most commonly used application layer protocols in the IoT domain, namely, Constrained Application Protocol (CoAP), Message Queuing Telemetry Transport (MQTT), Advanced Message Queuing Protocol (AMQP), and Extensible Messaging and Presence Protocol (XMPP). MQTT, AMQP, and XMPP use TCP for device-to-device communication, while CoAP utilizes UDP to achieve this purpose. MQTT and AMQP are based on a publish/subscribe model, CoAP uses the request/reply model for its structuring. In addition to this, the quality of service provision of MQTT, AMQP, and CoAP is not very high, especially when the deliverance of messages is concerned. The selection of protocols for each application is very a tedious task.This survey discusses the architectures, advantages, disadvantages, and applications of each of these protocols. The main contribution of this work is to describe each of the aforementioned application protocols in detail as well as providing their thorough comparative analysis. This survey will be helpful to the developers in selecting the protocol ideal for their system and/or application.

• 보건행정학회지
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• 제4권1호
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• pp.107-122
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• 1994

Many alternatives have been discussed to reduce the medical expenditure and to use the medical resources effectively. Many studies about the economies of scale have been done for the last several decades. This study has analyzed the relationship between the number of beds and the mean expense per hospitalization day in Korea. A Cost Function Model was identified and we wanted to see the minimum optimal size with the cheapest mean expense per hospitalization day. The result is as follows; 1. In the Cost Function Mode, (the number of beds)$^{2}$, the number of personnel, productivity and training institutions are the factors that statisticaly influence the mean expenses. 2. By the univariate analysis the mean expense proved to be the smallest as the level of 150-200bed, The breaked down of the components of expenses shows that the mean labor cost is much different from the mean value of material and administration costs, and that hospital with 150-200 beds also have the minimal expense. The mean expense goes up dramatically in hospitals of 450 beds or more. 3. When the other conditions are constant, according to the multiple regression analysis of the mean expense per adjusted hospitalization day the minimum optimal size with the cheapest expense is a hospital with 191 beds and the hospital with 230 beds takes the lowest mean labor cost. The material or administration costs are not influenced by hospital size. This research has limitation in measuring the variables that influence hospital xpenses, in estimating hospital output by the number of beds in considering outpatient cost and in securing representativeness of hospitals because many hospitals made no responses to the research questionnare. But it is valuable and helpful for development of health policy to figure out the number of beds with the cheapest expense per hospitalization day.