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Reproductive Efficiency and Characteristics of Cloned Miniature Piglets Produced from Domestic Commercial Gilts

  • You, Jin-Young;Jeon, Yu-Byeol;Hyun, Sang-Hwan;Park, Soo-Bong;Lee, Eun-Song
    • Journal of Embryo Transfer
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    • v.25 no.4
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    • pp.215-219
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    • 2010
  • The objective of this study was to examine the reproductive characteristics of cloned miniature piglets produced from surrogate domestic pigs. Somatic cell nuclear transfer (SCNT) miniature pig embryos were transferred into domestic pigs. As controls, domestic pigs of the same breed with surrogates for SCNT embryos and miniature pigs of the same breed with the somatic cell donor were bred by artificial insemination and natural mating, respectively. Surrogate domestic pigs that farrowed cloned miniature piglets had a significantly longer gestation length (118.1 days) than conventionally bred domestic (115.4 days) and miniature (115.5 days) pigs. Furthermore, the birth weight of cloned miniature piglets produced from domestic pigs (743 g) was significantly greater than that of miniature piglets produced by natural breeding (623 g). Also, cloned miniature piglets had a significantly lower weaning rate (49.7%) than conventionally produced domestic (91.5%) and miniature (100%) piglets. No differences were observed between female and male cloned piglets in gestation length, litter size, birth weight, or weaning rate. Our results demonstrate that gestation length is extended in domestic pigs that are transferred with SCNT miniature pig embryos and that cloned miniature piglets have increased birth weight and high pre-weaning mortality.

The Comparison of the Behavior between Miniature Pigs and Conventional Sows during Gestation to Lactation Period (미니돼지 및 일반 돼지의 임신기, 분만 및 포유기 중의 모돈의 행동학적 특성 비교)

  • Kang, Kyung-Won;Choi, Young-Hwan;Kim, Kwang-Ho;Kim, Yoo-Yong
    • Journal of Animal Science and Technology
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    • v.52 no.1
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    • pp.57-64
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    • 2010
  • This study was conducted to compare the aspects of behavior between miniature pigs and conventional sows during gestation and lactation periods. Miniature pigs and conventional sows at 3 parity were used in this experiment with 4 pigs per treatment. The behavioral patterns and stereotyped behaviors were observed on day 60 and 90 of gestation, at farrowing, and on day 10 of lactation before and after the feeding. On 60 and 90 days of pregnancy, both treatments showed the tendency of frequent lateral lying rather than other postures. On ventral lying, eating and drinking, miniature pigs spent more time than conventional pigs at day 90 of gestation (P<0.01, P=0.0539, P<0.05, respectively). The occurrence of stereotyped behaviors included bar biting and bar mouth chewing. At 90 day of pregnancy was observed, conventional pigs was higher than miniature pigs (P<0.05). At farrowing, miniature pigs spent more time on ventral lying, standing and walking than conventional sows (P<0.05), however, there were more frequency of drinking and eating. And on day 10 of lactation, there was no significant difference in stereotyped behaviors between miniature pigs and conventional sows. In conclusion, miniature pigs were generally more sensitive than conventional sows although sows were reared in stall during gestation and lactation period and the behavioral pattern during lactating period showed the similarity between miniature pigs and conventional sows for protection of nursing pigs. In consideration of the general reproductive performance of conventional sows, the high sensitivity and stress of miniature pigs did not affect the reproductive performance of miniature pigs.

A Study on the Effect of Blank Shape on the Miniature Stamping (미세스탬핑 공정에서 블랭크 형상의 영향에 관한 연구)

  • Shim Hyun-Bo
    • Transactions of Materials Processing
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    • v.15 no.4 s.85
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    • pp.304-310
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    • 2006
  • Due to a recent growth of the area of MEMS and a trend moving toward smaller scale, a micro manufacturing that is usually related with lithography is now emerging. Differently from traditional manufacturing processes, the micro or miniature manufacturing usually requires expensive sophisticated equipments and its characteristics are of high cost and of low productivity. However, a miniature stamping, which makes small sized product with a thin metal usually in the range of meso-scale, can be realized in a low cost and in a high productivity with relatively inexpensive equipments. For a successful development of miniature stamping, lots of obstacles, including material properties related with formability, have to be overcome. Since the thin metal shows distinctive characteristics, e.g., size effect and statistically scattered material properties, the formability of miniature stamping is not good in general and the possible shape with the miniature stamping is limited relatively simple shapes. Since the optimal blank improves formability and the improved formability can make up for problems of material properties, the possibility of success can be increased. This study is carried out to show the possibility of miniature stamping and to verify the effect of optimal blank for the miniature stamping.

Measurement of Material Properties for Miniature Stamping (미세 스탬핑용 박판소재의 물성치 측정)

  • Kim Y.S.;Shim H.B.
    • Transactions of Materials Processing
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    • v.15 no.3 s.84
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    • pp.247-254
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    • 2006
  • Rather than traditional manufacturing processes, miniature manufacturing processes usually require sophisticated equipments and characteristics of the processes of high cost and of low productivity. Contrarily, miniature stamping process can be realized in a low cost high productivity with relatively inexpensive equipments. In the meso scale, mechanical properties, especially work hardening characteristics, are discovered to be statically scattered and size dependent by intensive experimental and numerical investigations, which make the stamping process hard to apply to the miniature manufacturing. In this study, dual purpose experimental device that can be used for both miniature scale tensile test and miniature scale stamping by simple change of attachment has been developed. For the tensile test, the elongation has been measured with a combined use of a CCD camera and a linear encoder in order to account for the possibility of slippage between specimen and the grip and to ensure the accuracy of the measurement, while load has been measured with a load cell. To satisfy the required material properties for stamping, optimal annealing condition has been found by examining the microstructure of annealed specimen.

A Study on the Product Development for Wedding Miniature (웨딩 미니어쳐의 상품 개발에 관한 연구)

  • Kim, So-Young;Baik, Chun-Eui
    • Journal of the Korea Fashion and Costume Design Association
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    • v.13 no.4
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    • pp.153-165
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    • 2011
  • The purpose of this study is to take into consideration the package products of wedding miniature dress. The method of the research was mainly focused on precedent research data and general references. Furthermore the data on wedding dresses was mainly collected from internet sites. Ai; reflection customer's demands, more personal and distinctive design was planned reflecting trend in the sector of wedding dress. The results of the research is the following. First, the first consideration for designing product in wedding miniature was designed with the focus on what consumers are easy to make and on brilliance when having made. 8 pairs for barbie miniature and 2 pairs for ball joints were designed. Among these things, it designed colorfully with 6 pairs for wedding dress and 4 pairs for shooting, which are used in the right size. Second, as a result of seeing consumers' response by up-loading totally 10 pieces of miniature clothes on wedding miniature. com site, and were the most popular products. The aim is to suggest package product based on these two works. The design-based pattern, the fabric of being used, lace material, beads, and several trimmings are offered to 2 wedding miniature package products. Consumers can make own collection with a handicraft-based feeling by using this.

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Development and Application of a Miniature Stereo-PIV System (Miniature Stereo-PIV 시스템의 개발과 응용)

  • Kim, K.C.;Chetelat, Olivier;Kim, S.H.
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.11
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    • pp.1637-1644
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    • 2003
  • Stereoscopic particle image velocimetry is a measurement technique to acquire three dimensional velocity field by two cameras. With a laser sheet illumination, the third velocity component can be deduced from out-of$.$plane velocity components using a stereoscopic matching method. Most industrial fluid flows are three dimensional turbulent flows, so it is necessary to use the stereoscopic PIV measurement method. However the existing stereoscopic PIV system seems hard to use since it is very expensive and complex. In this study we have developed a Miniature Stereo-PIV(MSPIV) system based on the concept of the Miniature PIV system which we have already developed. In this paper, we address the design and some primitive experimental results of the Miniature Stereo-PIV system. The Miniature Stereo-PIV system features relatively modest performances, but is considerably smaller, cheaper and easy to handle. The proposed Miniature Stereo-PIV system uses two one-chip-only CMOS cameras with digital output. Only two other chips are needed, one for a buffer memory and one for an interfacing logic that controls the system. Images are transferred to a personal computer (PC) via its standard parallel port. No extra hardware is required (in particular, no frame grabber board is needed).

Assessment of Sperm Characteristics in Fresh and Frozen Semen of Miniature-Pig

  • Lee S. H.;Kim T. S.;Cheong H. T.;Yang B. K.;Kim C. I.;Park C. K.
    • Reproductive and Developmental Biology
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    • v.28 no.4
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    • pp.261-265
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    • 2004
  • The present study was conducted to assess sperm characteristics in miniature-pig. The semen samples were transported to the laboratory at 17℃ within 3 hours after collection. The extended semen was stored at 17℃, and sperm quality was evaluated at 0, 1, 3, 5 and 7 days after storage. The semen volume of miniature-pig (62±22㎖) was significantly (p<0.05) lower than that of Duroc (155±25㎖) and Yorkshire (154±23㎖). Significant differences were also observed in sperm concentrations. During 3 days of storage, sperm viability did not differ among miniature-pig, Duroc and Yorkshire. However, the viability was significantly (p<0.05) lower in miniature-pig than in Duroc and Yorkshire semen after Day 3 of storage. In abnormality, acrosome intactness and intensity, there were no differences among miniature-pig, Duroc and Yorkshire semen. On the other hand, the viability of frozen-thawed sperm in miniature-pig was significantly (p<0.05) lower than in that of Duroc and Yorkshire. This study also examined CTC patterns in frozen-thawed spermatozoa. The rates of AR pattern were higher in miniature-pig than in Duroc and Yorkshire. However, no difference was found in F, B and AR patterns. The results of present study suggest that further research is necessary to develop of semen extender and freezing methods to improve sperm quality in miniature-pig.

Analysis of Failure in Miniature X-ray Tubes with Gated Carbon Nanotube Field Emitters

  • Kang, Jun-Tae;Kim, Jae-Woo;Jeong, Jin-Woo;Choi, Sungyoul;Choi, Jeongyong;Ahn, Seungjoon;Song, Yoon-Ho
    • ETRI Journal
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    • v.35 no.6
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    • pp.1164-1167
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    • 2013
  • We correlate the failure in miniature X-ray tubes with the field emission gate leakage current of gated carbon nanotube emitters. The miniature X-ray tube, even with a small gate leakage current, exhibits an induced voltage on the gate electrode by the anode bias voltage, resulting in a very unstable operation and finally a failure. The induced gate voltage is apparently caused by charging at the insulating spacer of the miniature X-ray tube through the gate leakage current of the field emission. The gate leakage current could be a criterion for the successful fabrication of miniature X-ray tubes.

Normal Lung Ventilation/Perfusion Scintigraphy in Miniature Pigs (미니돼지에서 정상 폐 환기/관류 신티그라피)

  • Kim, Se-Eun;Han, Ho-Jae;Shim, Kyung-Mi
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1725-1728
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    • 2010
  • Miniature pigs are widely used in experiments related to pulmonary disease because of their similarities with humans. However, there are not enough data about normal lung function in miniature pigs. Thus, in this study, we investigated normal lung function in miniature pigs with lung ventilation/perfusion scintigraphy and evaluated the availability of this method. Three male miniature pigs weighing 30-35 kg were used. After general anesthesia, ventilation scintigraphy was performed with 100 MBq of $^{99m}Tc$-pertechnetate (${O_4}^-$), after which perfusion scintigraphy was performed with intravenous injection of $^{99m}Tc$-macro aggregated albumin (MAA). The functional contribution of the right lung was about 55%, and left lung was about 45%, similar to humans. Lung ventilation/perfusion scintigraphy was very useful in evaluating the normal lung function of miniature pigs because it was a non-invasive procedure (no tissue damage was involved), took a short time and was easy to perform. In conclusion, miniature pigs are similar to humans in functional contributions of the lung, and this method will be helpful in future pulmonary disease studies involving miniature pigs.

Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites

  • Wu, Jinhua;Liu, Ronghui;Li, Hua;Yu, Hui;Yang, Yalan
    • Animal Bioscience
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    • v.34 no.11
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    • pp.1757-1765
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    • 2021
  • Objective: The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. Methods: Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. Results: In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. Conclusion: This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs.