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Assessment of Sperm Characteristics in Fresh and Frozen Semen of Miniature-Pig  

Lee S. H. (College of Animal Resource Science, Kangwon National University)
Kim T. S. (College of Animal Resource Science, Kangwon National University)
Cheong H. T. (College of Animal Resource Science, Kangwon National University)
Yang B. K. (College of Animal Resource Science, Kangwon National University)
Kim C. I. (College of Animal Resource Science, Kangwon National University)
Park C. K. (College of Animal Resource Science, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.28, no.4, 2004 , pp. 261-265 More about this Journal
Abstract
The present study was conducted to assess sperm characteristics in miniature-pig. The semen samples were transported to the laboratory at 17℃ within 3 hours after collection. The extended semen was stored at 17℃, and sperm quality was evaluated at 0, 1, 3, 5 and 7 days after storage. The semen volume of miniature-pig (62±22㎖) was significantly (p<0.05) lower than that of Duroc (155±25㎖) and Yorkshire (154±23㎖). Significant differences were also observed in sperm concentrations. During 3 days of storage, sperm viability did not differ among miniature-pig, Duroc and Yorkshire. However, the viability was significantly (p<0.05) lower in miniature-pig than in Duroc and Yorkshire semen after Day 3 of storage. In abnormality, acrosome intactness and intensity, there were no differences among miniature-pig, Duroc and Yorkshire semen. On the other hand, the viability of frozen-thawed sperm in miniature-pig was significantly (p<0.05) lower than in that of Duroc and Yorkshire. This study also examined CTC patterns in frozen-thawed spermatozoa. The rates of AR pattern were higher in miniature-pig than in Duroc and Yorkshire. However, no difference was found in F, B and AR patterns. The results of present study suggest that further research is necessary to develop of semen extender and freezing methods to improve sperm quality in miniature-pig.
Keywords
Miniature-pig; Semen characteristics; Frozen-thawing; CTC pattern;
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