• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.581-586
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• 1997

The present study analysed the various productive and reproductive characteristics of 1/2 Pabna Milking Cows (PMC)-1/2 Sahiwal (S) and 1/4 PMC-3/4 S collected from the Pabna milkshed area at Baghabarighat, Sirajgonj, Bangladesh. The studied traits were birth weight (BWT), age at puberty (AP), number of services per conception (NSC), post partum heat period (PPHP), gestation period (GP), daily milk yield (DMY), lactational production (LP), lactation length (LL), fat percentage and solids-not-fat percentage (SNFP). Five individual Sahiwal sires were used for the upgrading of Pabna Milking Cows. The used data covered from 1987 to 1994. Least-squares analysis of variance showed that genetic group had a significant effect on BWT (P < 0.05), AP (p < 0.01), DMY (p < 0.01), LP (p < 0.001), LL (p < 0.05), FP (p < 0.05) and SNFP (p < 0.01). Genetic group had non-significant effect on NSC, GP and PPHP. The effect of sire was significant on BWT (p < 0.01), NSC (p < 0.01), LP (p < 0.05) and LL (p < 0.05). The AP, NSC, DMY, LP, LL and SNFP were higher in 1/2 PMC-1/2S cows; BWT and PPHP were higher in 1/4 PMC-3/4S but GP and FP were almost same in both genetic groups. From this study it may be concluded that production and use of 1/2 PMC-1/2S would seem more profitable for commercial milk production in the Bangladesh Milk Producers' Cooperative Union Limited (BMPCUL) area and at the same time emphasis should be given on rigorous sire selection.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.290-296
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• 2005

This study was carried out to develop the efficient freeze concentration process of milk through controlling the recrystallisation phenomena of ice. Freeze-concentration was progressed with multi-stage freeze concentrator and there was artificial temperature control to induce recrystallisation phenomena. In each stage of freeze concentration process, the regular recrystallisation time was fixed as 1, 2, 4 and 8 hr to compare the solute increment, yield, brix and ice-crystal size among experimental conditions. Higher concentration as total solids was observed due to the elapse of recrystallisation time, and the maximum total solids in final products: 32.67% was obtained at the ripening time of 8 hr in two-stage process. This result was excessively high concentration comparing to the existing researches and presented the possibilities of milk freeze concentration in the dairy industry, The results of brix and ice-crystal size showed the direct correlation with the recrystallisation time that meant the increased processing time showed the increment of brix and ice-crystal size. Obtained results were numerically modelled to predict the progress of concentration in the industrial process and all of them had fairly high R2 of determination. Therefore, we regarded that these numerical models could be utilized for the development of efficient technology in industrial freeze concentration process.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.509-514
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• 2007

In this study, we optimized the production of ${\gamma}-polyglutamic$ acid (PGA) in soybean milk cakes (SMC) fermented with Bacillus subtilis GT-D and B. subtilis KU-A, to be utilized as a functional food ingredient. PGA production was dependent upon the glutamate content, fermentation time, and type of Bacillus sp. The consistencies of the SMCs fermented by B. subtilis GT-D and B. subtilis KU-A were highest after 36 hr of fermentation, and then decreased gradually. The SMC fermented by B. subtilis KU-A had a higher consistency than the SMC fermented by B. subtilis GT-D. In the presence of 10% defatted soy flour (DFS), 5% glutamate in the SMC was efficiently converted into polyglutamic acid (PGA) for 24 hr, indicating a conversion yield above 96%, but its conversion then decreased with higher concentrations of glutamate. The soluble solid content (mucilage) of the SMC fermented with B. subtilis KU-A was 9.5%(w/w), and composed of 65.6% PGA (Mw 1,536 kDa) and some polysaccharides. However, the SMC fermented with B. subtilis GT-D had a mucilage content of 7.8%(w/w), and was composed of 66.4% PGA (Mw 1,409 kDa), 11.5% levan, and some polysaccharides. The viscoelastic values of the mucilage obtained using B. subtilis KU-A were much higher than those of mucilage obtained using B. subtilis GT-D. Also, the G'-value (elastic modulus) was higher than the G"-value (viscous modulus).

• Korean Journal of Food Science and Technology
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• v.20 no.2
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• pp.245-251
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• 1988

Thermolysin was immobilized on Dowex MWA-1 with 10% glutaraldehyde and incorpo rated into a fluidized-bed continuous coagulation scheme to make Cheddar type cheese. The activity yield of thermolysin was 25%. The immobillized thermolysin was stable at $60^{\circ}C$ in the presence of 1/200M calcium ions and the half-life value is 16 days at the temperature. Raw milk alkalified to pH 7.0 was passed through a column of thermolysin beads at $55^{\circ}C$, cultivated with Streptococcus cremoris and allowed to coagulate. A typical milk curd was formed to make Cheddar type cheese, avoiding troublesome microbial contamination successfully during continuous hydrolysis process. During ripening of this cheese for 6 months at $10^{\circ}C$, its ripening ratio and taste were similar to those of cheese prepared by the traditional method.

• Animal Bioscience
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• v.34 no.4
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• pp.759-769
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• 2021

Objective: This study aimed to investigate the effect of stocking density on the behavior, productivity, and metabolism of periparturient Holstein cows as well as calf performance. Methods: A total of 48 periparturient cows were randomly assigned into three groups at 28 days (±3 days) before their expected calving date. The stocking densities of the groups, relative to the standard cubicle and feed bunk number, were i) 80% (13 cows), ii) 100% (16 cows), and iii) 120% (19 cows). Lying and rumination behavior was recorded using electronic data loggers and HR-Tags from d -21 ("d-" means days before calving) until the calving date, d 0. Lying time was assessed to determine the diurnal total hours spent lying per day. Rumination time was averaged in 2 hours interval periods over 24 hours during the experimental period. Results: Cows in the 80% group spent more time lying and ruminating between d -21 and d -7 and tended to ruminate more between d -14 and d 0. Calcium levels tended to be higher for cows in the 80% group, no other observable differences were found in monitored blood parameters. Moreover, 3.5% fat corrected milk and energy corrected milk yields were higher in 80% group in the first month of lactation. No other observable differences were found in the yield and composition of colostrum and milk in the first 10 months of lactation. The growth and performance of calves in the first week of life was not affected by stocking density of the dams. Conclusion: We concluded that lower stocking density may increase lying and ruminating behavior of prepartum Holstein cows. However, this did not translate into improved productivity and metabolism.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.12
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• pp.1657-1667
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• 2010

Direct-fed microbials (DFM) are dietary supplements that inhibit gastrointestinal infection and provide optimally regulated microbial environments in the digestive tract. As the use of antibiotics in ruminant feeds has been banned, DFM have been emphasized as antimicrobial replacements. Microorganisms that are used in DFM for ruminants may be classified as lactic acid producing bacteria (LAB), lactic acid utilizing bacteria (LUB), or other microorganisms including species of Lactobacillus, Bifidobacterium, Enterococcus, Streptococcus, Bacillus and Propionibacterium, strains of Megasphaera elsdenii and Prevotella bryantii and yeast products containing Saccharomyces and Aspergillus. LAB may have beneficial effects in the intestinal tract and rumen. Both LAB and LUB potentially moderate rumen conditions and improve feed efficiency. Yeast DFM may reduce harmful oxygen, prevent excess lactate production, increase feed digestibility, and improve fermentation in the rumen. DFM may also compete with and inhibit the growth of pathogens, stimulate immune function, and modulate microbial balance in the gastrointestinal tract. LAB may regulate the incidence of diarrhea, and improve weight gain and feed efficiency. LUB improved weight gain in calves. DFM has been reported to improve dry matter intake, milk yield, fat corrected milk yield and milk fat content in mature animals. However, contradictory reports about the effects of DFM, dosages, feeding times and frequencies, strains of DFM, and effects on different animal conditions are available. Cultivation and preparation of ready-to-use strict anaerobes as DFM may be cost-prohibitive, and dosing methods, such as drenching, that are required for anaerobic DFM are unlikely to be acceptable as general on-farm practice. Aero-tolerant rumen microorganisms are limited to only few species, although the potential isolation and utilization of aero-tolerant ruminal strains as DFM has been reported. Spore forming bacteria are characterized by convenience of preparation and effectiveness of DFM delivery to target organs and therefore have been proposed as DFM strains. Recent studies have supported the positive effects of DFM on ruminant performance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.1
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• pp.29-33
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• 2016

In order to provide basic data to predict changes of milk components in milk yield, this study was conducted to compare nutritive values of diets collected from organic dairy farms (ODF) and conventional dairy farms (CDF) of Chungnam and Jeonbuk in Korea. The contents of crude fat, crude fiver, neutral detergent fiber, and acid detergent fiber in diets of ODF were significantly (p<0.05) higher compared to those of CDF. The contents of crude protein and crude ash in ODF were also higher (p<0.05) than those of CDF. The contents of calcium, ferrum, and phosphorus in diets of ODF were similar to those of CDF. The contents of potassium, magnesium, and iodine in diets of ODF were higher (p<0.05) than those of CDF. The contents of total saturated and unsaturated fatty acids in diets of ODF were higher (p<0.05) compared to those of CDF. The contents of oleic acid, linoleic acid, and linolenic acid in diets of ODF were higher (p<0.05) compared to those of CDF. However, the contents of myristic acid, palmitic acid, and stearic acid in diets of ODF were lower (p<0.05) compared to those of CDF.

• Journal of Practical Agriculture & Fisheries Research
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• v.8 no.1
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• pp.43-51
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• 2006

An experiment was conducted to examine how the response of dairy cows to a change from twice to thrice-daily milking is affected by deficiencies in the dietary supplies of three amino acids, His, Met and Lys. The amino acid deficiencies were obtained by feeding the cows a diet of grass silage and a cereal-based supplement containing feather meal as the sole protein supplement. Taken overall, the results show that when cows were given the feather meal diet, even though dietary ME was in considerable excess, a deficiency of specific amino acids prevented any increase in milk yield in response to increasing the frequency of milking from twice to thrice daily. The results of half-udder milking showed that when cows consumed the diet deficient in amino acids, milking one half of the gland more frequently reduced the secretion of protein and lactose by the control gland. Neither MBF nor the ratio of 3-MH/creatinine in urine was affected by thrice-daily milking. The present results go a stage further in showing that, against a background of insufficient dietary amino acids, the stimulus of thrice-daily milking is not sufficient to induce a measurable change in the partition of amino acid use between body and udder.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.267-274
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• 2010

The objective of this experiment was to investigate effects of total mixed ration (TMR) of different nutrition level on milk production in dairy goats (Sannen). Twenty four lactating goats were randomly assigned to one of four TMRs; medium energy-medium crude protein (CP) TMR (control), high energymedium CP TMR (T1), medium energy-high CP TMR (T2) and high energy-high CP TMR (T3). The content of total digestible nutrients (TDN) and CP in the control diet were 67.0% and 13.9%. The TDN content of the high energy TMR was 73.7% and the CP content of the high CP TMR was 15.3%. Milk yield was significantly (P<0.05) higher for T1 ($2,198{\pm}115 ml$) than for T2 ($1,742{\pm}52 ml$) and T3 ($1,984{\pm}90 ml$). But there were no significant differences in milk composition between the treatments. Daily body weight gain was increased in T1 and T3 but reduced in control and T2. The result of the present study showed that dietary energy level supplied more than that of NRC recommendation did not affect milk yield of dairy goats in early lactation but prevented body weight loss.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1600-1608
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• 2005

The study was carried out to evaluate the effect of exogenous bovine somatotropin on water metabolism in relation to mammary function in early lactation of crossbred Holstein cattle. Ten, 87.5% crossbred Holstein cattle were divided into two groups of 5 animals each. At day 60 of lactation, the control group was given placebo while animals in the experimental group were given recombinant bovine somatotropin (rbST) by subcutaneous injection with 500 mg of rbST (14-days prolonged-release rbST). In rbSTtreated animals, milk yield increased 19.8%, which coincided with a significant increase in water intake (p<0.01), while DM daily intake was not different when compared to the control animals. Water turnover rate as absolute values significantly increased (p<0.05), while the biological half-life of water did not change in rbST-treated animals. Total body water (TBW) and total body water space (TOH) as absolute values significantly increased (p<0.01) in rbST-treated animals, while it was decreased in the control animals. Absolute values of empty body water (EBW) markedly increased (p<0.05), which was associated with an increase in the extracellular fluid (ECF) volume. Absolute values of plasma volume and blood volume were also significantly increased (p<0.05) in rbST-treated animals. The increase in mammary blood flow in rbST-treated animals was proportionally higher than an increase in milk production. The plasma IGF-1 concentration was significantly increased (p<0.01) in rbST-treated animals when compared with those of control animals during the treatment period. Milk fat concentration increased during rbST treatment, while the concentrations of both protein and lactose in milk were not affected. The present results indicate that rbST exerts its effect on an increase in both TBW and EBW. An increased ECF compartment in rbST-treated animals might partly result from the decrease in fat mass during early lactation. The action of rbST on mammary blood flow might not be mediated solely by the action of IGF-1 for increase in blood flow to mammary gland. An elevation of body fluid during rbST treatment in early lactation may be partly a result of an increase in mammary blood flow in distribution of milk precursors to the gland.