• Title/Summary/Keyword: microscopic system

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Determination of best enrichment media for growth of Salmonella injured from cold temperature during process and storage (저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구)

  • Park, Mi-Kyung
    • Food Science and Preservation
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    • v.23 no.6
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    • pp.759-764
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    • 2016
  • This purpose of this study was to determine the best enrichment medium for rejuvenating and recovering Salmonella placed in cold temperature prior to the employment of the gold biosensor combined with a light microscopic imaging system. A mixture of nalidixic-resistant Salmonella Typhimurium and Enteritidis were inoculated onto chicken (1,000 CFU/chicken). After cold injury at $4^{\circ}C$ for 24 hr, Salmonella on chicken was enriched for 6 hr with six non-selective media including buffered peptone water broth, lactose broth, brain heart infusion broth (BHI), universal pre-enrichment broth, nutrient broth, and tryptic soy broth, and five selective media including brilliant green broth (BG), rappaport-vassiliadis R10 broth, selenite cystine broth, selenite broth, and tetrathionate brilliant green broth (TBG) for the comparison of Salmonella growth. Various concentrations of Salmonella (10, 50, 100, 500, and 1,000 CFU/chicken) were then enriched for 6 hr in both BHI and BG media to select the best media. BHI was selected as the most effective non-selective enrichment medium, while BG was selected as the most effective selective enrichment medium. Finally, BHI medium was selected as the most efficient enrichment medium for Salmonella growth injured from cold temperature during processing or storage.

Study on the Development of an Expressway Hard Shoulder Running Algorithm Using Reinforcement Learning (강화학습 기반 고속도로 갓길차로제 운영 알고리즘 개발 연구)

  • Harim Jeong;Sangmin Park;Sungkwan Kang;Ilsoo Yun
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.22 no.4
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    • pp.63-77
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    • 2023
  • This study applies reinforcement learning to effectively operate expressway hard shoulder running (HSR). An HSR algorithm was developed, and its effectiveness was evaluated using the VISSIM microscopic simulation program. The simulation evaluated two aspects: mobility and safety. The DQN-based HSR algorithm found speed improvement of up to 26 km/h. Compared to the current method, the difference in the number of conflicts was not significant. Considering the results, a DQN-based HSR operation has a clear effect, and it is necessary to consider adjusting the current operational criteria.

MULTI-SHAPE ERYTHROCYTE IMAGING AND THEIR DEFORMABILITY ANALYSIS IN A MICROFLUIDIC ENVIRONMENT

  • Jayavanth, Sanjay;Pak, Bock-Choon
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2791-2796
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    • 2007
  • In their long journey through the cardiovascular circuit, erythrocytes are coerced to shape transform and assume different shapes on account of varying flow conditions in different blood vessels. The present work was aimed to visualize these erythrocyte shape transformations by an invitro microcirculatory model, and assess multi-shape erythrocyte deformability. The model uses an in-house fabricated, inexpensive disposable micro flow channel to mimic certain invivo conditions and a fast frame video microscopic system for imaging the shape changes in erythrocytes. Results show the multi-shape transformation of erythrocyte christened as discoidal shape, the asymmetrically deformed 'hat' and 'bullet-like' shapes, and the axially deformed 'slipper' and 'spindle-like' shapes. Specific erythrocyte showed the shape transition and transformation while passing through the observed window. The obtained erythrocyte shapes very analyzed for deformability index using image processing techniques that varied significantly (p <0.001) for different shapes as compared with the resting shape.

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A study on real time measuring microscopic movements in sinusoidal phase modulating interferometry using a laser diode (레이저 다이오드를 이용한 정현파 위상변조 간섭에 의한 실시간 극미세 진동 측정에 관한 연구)

  • 구자남;염정덕;지철근
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1990.10a
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    • pp.23-27
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    • 1990
  • A new signal processing system for real time displacement measurement in sinusoidal phase modulating interferometry is described. Although sinusoidal phase modulating interferometry is effective in measuring with high accuracy the displacement of an object, conventional signal processing takes a long time. In this method, detection of the object's displacement is easily achieved by sampling the interference signal at those times that satisfy certain conditions and by processing the sampled signals with electric circuits in real time. The delay time of this signal processing system is < 45${\mu}$s. Specially in this paper we describe all electronic circuit and optical system design

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A Study on the Neurotoxic Effects of Tellurium on Murine Nervous System (랫드의 신경조직에 미치는 Tellurium의 독성에 관한 연구)

  • 김기석;정문호
    • Journal of Environmental Health Sciences
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    • v.24 no.3
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    • pp.35-40
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    • 1998
  • This study was conducted to examine the pathological changes of rat peripheral nervous system during exposure to tellurium known to be a demyelinating agent by using teasing nerve fiber method and quantitative light microscopic analysis by image analyzer. The pellet containing 1.2% of tellurium were fed for 3, 5, 7, 9, 13 days to male wistar rats (21 days old) and then neurologic symptom and the feature of nerve fiber myelination were studied. From this study, following results were obtained. In 3 days treated group, it showed various neurologic symptom and teased nerve fiber showed slight irregularity of the myeline sheath. In 5 days and 7 days treated groups, it showed the segmental demyetination in larger size fiber and widening of nodes of ranvier. In 9 days and 13 days treated groups, the remyelinated fibers were observed and it was generally small in size. We consequently suggest that teasing nerve fiber method and quantitative analysis of nerve fiber were useful pathologic screening method of neurotoxicity of the peripheral nervous system.

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A Study on the Detail Conception of Korean Electrical Safety System Based on Comparing with Developed Countries by the Microscopic Analysis of Electricity Accidents (국내 전기안전시스템의 미시적 재해분석을 통한 선진국 전기안전시스템과 비교를 기반으로 한 국내전기안전시스템의 개념적 설계)

  • Park, Dong-Jun;Kim, Jung-Hoon;Park, Jung-Wook
    • Proceedings of the KIEE Conference
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    • 2007.11b
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    • pp.322-324
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    • 2007
  • In this paper conceptual design methodologies of electric safety systems are proposed by micro and macro views. As a pre-study electric accidents of Korea were analyzed microscopically, which could be categorized into 11 types by the occurrence frequency. These types are caused by common problems, which are educations, live works, dealing by the public, and grounding systems. And the korean electric safety system is analyzed macroscopically according to stages of the system comparing with that of developed countries.

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A study on real time measuring microscopic movements in sinusoidal phase modulating interferometry using a laser diode (레이저 다이오드를 이용한 정현파 위상변조 간섭에 의한 실시간 극미세 진동 측정에 관한 연구)

  • 강성철;진상규;지철근
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1991.10a
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    • pp.33-37
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    • 1991
  • A now signal precessing system for real time displacement measurement in sinusoidal phase modulating interferometry is described. Although sinusoidal phase modulating interferometry is effective in measuring with high accuracy the displacement of an object, conventional signal processing takes along time. In this method, detection of the object's displacement is easily achieved by sampling the interference signal at those times that satisfy certain conditions and by processing the sampled signals with electric circuits in real time. the delay time of this signal processing system is < 45 $\mu$s. Specially in this paper we describe all electronic circuit and optical system design

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A study on the model of the information service in the taylorian researcher's cognitive viewpoint (테일러(R. S. Taylor)계 연구자 관점에서의 정보요구 및 탐색모형에 관한 연구)

  • 이승채
    • Journal of Korean Library and Information Science Society
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    • v.22
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    • pp.245-276
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    • 1995
  • This study analyzed the process of information service and its factors in the cognitive viewpoint. And developed a conceptual model to help in developing future information systems and services. The results of this study were summarized as follows : 1) The paradigm of information user study is changing from the system-centered to the user-centered one. 2) The researchers describe the user's internal cognitive process as the starting point of the information service. 3) The user's personal cognitive process influence the process of the information service over. And the focus is a microscopic one including the factors of the perception and recognition of the stimulus, remembering and searching information, reasoning the rules, recognition of the patterns, formalization of the concepts, etc. 4) The information librarian performs the role as a knowledge/ information system interface, and his knowledge structure reflects the organization of that knowledge/information system. 5) The information librarians can perform their role as information specialists actively and offensively. And the performance of the librarians can be measured on the scales of the su n.0, pplying capability of relevant information and the client satisfaction.

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컴퓨터 영상처리에 의한 윤활시스템의 상태진단

  • 서영백;박흥식;전태옥;이충엽
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 1997.04a
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    • pp.224-231
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    • 1997
  • Microscopic examination for the morphological estimation of wear debris on the oil-lubrcated moving system is an accepted method for machine condition and fault diagnosis. However wear particle anaysis has not been widely accepted industry because it is dependent on expert interpretation of particle morphology and relies on subjective assessment criteria. This paper was undertaken to estimate the morphology of wear debris on the oil-lubricated movig system by computer image analysis. The wear test was performed under different sliding conditions using a wear test device made in our laboratory and wear testing specimen of the pin-on-disk-type was rubbed in pararline series base oil. In order to describe characteristics of debris of various shape and size, four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) have been developed and outlined in the paper. A system using such techniques promises to obviate the need for subjective, human interpretation of particle morphology in machine condition monitoring.

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Multiple Vision Based Micromanipulation System for 3D-Shaped Micro Parts Assembly

  • Lee, Seok-Joo;Park, Gwi-Tae;Kim, Kyunghwan;Kim, Deok-Ho;Park, Jong-Oh
    • 제어로봇시스템학회:학술대회논문집
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    • 2001.10a
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    • pp.103.5-103
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    • 2001
  • This paper presents a visual feedback system that controls a micromanipulator using multiple microscopic vision information. The micromanipulation stations basically have optical microscope. However the single field-of-view of optical microscope essentially limits the workspace of the micromanipulator and low dept-of-field makes it difficult to handle 3D-shaped micro objects. The system consists of a stereoscopic microscope, three CCD cameras, the micromanipulator and personal computer. The use of stereoscopic microscope which has long working distance and high depth-of-field with selective field-of-view improves the recognizability of 3D-shaped micro objects and provides a method for overcoming several essential limitations in micromanipulation. Thus, visual feedback information is very important in handling micro objects for overcoming those limitations and provides a mean for the ...

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