• Corrosion Science and Technology
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• v.17 no.1
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• pp.1-5
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• 2018

As structural materials, magnesium (Mg) alloys have been widely used in the fields of aviation, automobiles, optical instruments, and electronic products. There are few studies on the effect of coating conditions on the compositional variation during the formation process of the conversion coatings. Rare-earth based conversion coating on AZ91 magnesium alloy was prepared in ceric sulfate and hydrogen peroxide contained solution. The element composition and valence as well as their distribution in the coating were analyzed with energy dispersive X-ray spectroscopy (EDS), Electron probe micro-analyzer (EPMA), X-ray photoelectron spectroscopy (XPS). The effect of treating process on the element composition were also studied. It was found that the conversion coating surface consists of Mg, Al, O, Ce, and the weight content of Ce in the coating was affected by the treating solution concentration and immersion time; the Ce element was distributed in the coating non-uniformly and existed in the form of $Ce^{+3}$ and $Ce^{+4}$, while the O element existed in the form of $OH^-$, $O^{2-}$, $H_2O$. Based on microscopic analysis results, the electrochemical deposition mechanism on the micro-anode and micro-cathode in the process of the coating growth was suggested.

• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• Journal of fish pathology
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• v.36 no.2
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• pp.303-310
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• 2023

Yellow goosefish (Lophius litulon) is one of the important commercial fisheries target species in Korea, and commonly consumed as braised or stew. The microsporidian Spraguea is known to infect the nervous system of lophiid fish, forming numerous visible whitish xenomas. This parasite is commonly found in lophiid fish worldwide, but there is no information on the infection status of this parasite in Korea. We obtained commercially available chopped packs of lophiid fish from several fish markets and investigated their prevalence of infection. The isolated xenomas were crushed and purified as mature spore suspension. Microscopic observation and PCR were conducted to visualize and identify them. The host fish was also identified by DNA bar cording analysis. All the specimens were heavily infected and microscopic observation with Giemsa or Chromotrope 2R stain revealed tiny oval shapes of typical microsporidian spores. PCR analysis targeting the partial SSU rDNA showed that our specimen belongs to the genus Spraguea clade. But clear identification at the species level was not possible, due to the insufficient information of gene sequences available in GenBank. In addition, all of our host fish specimen was identified as yellow goosefish. This is the first report of a microsporidian parasite Spraguea infection in yellow goosefish from Korea.

• Journal of The Korean Association For Science Education
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• v.27 no.4
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• pp.337-345
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• 2007

The purposes of this study are to investigate and compare the characteristics of classification criteria of secondary school students and science teachers in relation to the three statesof matter. For this research, 76 middle school students in the7th grade who studied microscopic viewpoints about classifying the state of matter for the first time, 69 high school students in the 12th grade who studied Chemistry I and IIcourses successfully, and 130 secondary school science teachers, through a questionnaire. As a result, while having learned the three states of matter in the microscopic viewpoints, the majority of middle school students showed a high percentage of classification in the macroscopic viewpoints. For high school students, their percentage of classification included highmicroscopic viewpoints about solid and gas, the percentage of classification included macroscopic viewpoints about liquid state as alsohigh. The secondary school teachers gave answers in the macroscopic viewpoints and microscopic viewpoints equally, but their answers were just representation of the viewpoints list without the meaningful connection of the two type of viewpoints. To solve these problems, It is necessary to form connective change from the macroscopic viewpoints to the microscopic viewpoints about the criteria for classifying the States of Matter and various educational efforts.

• Journal of Korean Society of Transportation
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• v.25 no.2 s.95
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• pp.73-81
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• 2007

The purpose of this paper is to perform a basic study on the interaction between lanes, which can be achieved through analyzing traffic breakdown at the microscopic level. Using aerial photographic data for the microscopic analysis, this study analyzed the characteristics of traffic flow at a merging area. This research produced aggregated traffic data such as flows, speeds, and densities in 30 second intervals by lane for the macroscopic analysis and individual headway data by lane for the microscopic analysis. The paper contains an analysis of lane characteristics through flows, speeds, densities, and headway variations and also investigates the influence of ramp flows on mainline flows with space-time diagrams. Firstly, the merging area in this study is divided into three sections: before-merging, during-merging, and after-merging. The transition process was analyzed at each lane. Secondly, the breakdown was observed in detail with data divided in 50-foot units. The breakdown was checked through the relationships between ramp and freeway mainline flows, various techniques were proposed to analyze the breakdown, and the formation of breakdown was introduced as three stages in this study. In the near future, the findings of this study could contribute to determining the dynamic capacity on freeways by easily understanding changeable traffic breakdown patterns over time and space.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.10 no.4
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• pp.107-115
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• 2011

Since 2009 The Korean National Police Agency has been pushed "permissive left-turn" forward in order to making progress in Korean traffic operations system. Preceding researches manuals and guidelines present 6 standards(# of accidents # of lanes, pedestrian volume sight # of permissive left-turn lanes vehicle volume) as installation permissive left-turn. But in practical affairs it is most important that secure enough Gap-time between permissive left-turn vehicle and opposite through lane vehicle to make permissive left-turn vehicle move safer and more efficiently. This study suggests applying gap acceptance theory in microscopic model to permissive left-turn installation standards. Analysis methods of this study are field data survey statistical analysis and microscopic simulation analysis. This study collected field data by using AVI recording and measured permissive left-turn vehicle intersection passing time(T1) and against the opposite through lane vehicle Gap-time(T2). And statistical analysis performed about two values that measured before to predict the functionality between T1 and T2. These studies to overcome the limit of sample size carried out a microscopic simulation(VISSIM) plan and collect more samples to input statistical analysis.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2019.11a
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• pp.176-177
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• 2019

As a result of the Rietveld analysis to determine the effect of carbon nanotubes on the hydration products of cement composites, the quantitative difference of hydration products according to the addition rate of carbon nanotubes was not significant. Ettringite, an early hydration product, was measured to be slightly higher than the planes with carbon nanotubes over all ages. Therefore, it seems that carbon nanotubes have no effect on the hydration production in cement paste.

• Journal of Pharmacopuncture
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• v.25 no.4
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• pp.390-395
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• 2022

Objectives: Sweet bee venom (sBV) is purified from Apis mellifera, containing a high level of melittin-its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeutic application of sBV for anticancer treatment. In the present study, we investigated the pharmacological effect of sBV treatment in A549 human lung cancer cells. Methods: We used microscopic analysis to observe the morphological changes in A549 cells after sBV treatment. The MTT assay was used to examine the cytotoxic effect after dose-dependent sBV treatment. Molecular changes in sBV were evaluated by the expression of apoptosis marker proteins using western blot analysis. Results: Microscopic analysis suggested that the growth inhibitory effect occurred in a dose-dependent manner; however, cell lysis occurred at a concentration over 20 ㎍/mL of sBV. The MTT assay indicated that sBV treatment exhibited a growth inhibitory effect at a concentration over 5 ㎍/mL. On fluorescence activated cell sorting analysis, G0 dead cells were observed after G1 arrest at treatment concentrations up to 10 ㎍/mL. However, rapid cell rupture was observed at a concentration of 20 ㎍/mL. Western blot analysis demonstrated that sBV treatment modulated the expression of multiple cell death-related proteins, including cleaved-PARP, cleaved-caspase 9, p53, Bcl2, and Bax. Conclusion: sBV induced cell death in A549 human lung cancer cells at a pharmacological concentration, albeit causing hemolytic cell death at a high concentration.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.6 no.3
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• pp.57-66
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• 2007

Weaving is defined as the crossing of two or more traffic streams traveling in the same general direction along a significant length of highway without the aid of traffic control devices. Compared with other freeway sections, perturbation is easy to happen at weaving section. Because there are a lot of lane-changing maneuvers at the weaving section, traffic is subject to turbulence in excess of that normally presents on freeway basic section. This turbulence causes operational problems and its impact must be considered. The purpose of this paper is to perform a basic study on flow characteristics by lane, which can be achieved through analyzing breakdown phenomenon in the microscopic approach. The study made use of data derived from the aerial photography for the microscopic analysis. This research produced the 30-second interval data such as flows, speeds, and densities for the macroscopic analysis and derived the vehicular data to draw time-space diagram for the microscopic analysis. The paper analyzed the traffic characteristics using flows, speeds and densities variation and investigated the conditions of breakdown occurrence with the time-space diagrams. The breakdown phenomenon was identified at weaving section and the propagation from free flow to synchronized flow was observed in this study. In the future, the findings help develop the traffic operational algorithm to manage the traffic congestion under ubiquitous circumstance since the conditions of breakdown Phenomenon can be understood more.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.10 no.4
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• pp.11-23
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• 2011

The objective of this paper is to develop a daily pattern clustering algorithm using historical traffic data that can reliably detect under various traffic flow conditions in urban streets. The developed algorithm in this paper is categorized into two major parts, that is to say a macroscopic and a microscopic points of view. First of all, a macroscopic analysis process deduces a daily peak/non-peak hour and emphasis analysis time zones based on the speed time-series. A microscopic analysis process clusters a daily pattern compared with a similarity between individuals or between individual and group. The name of the developed algorithm in microscopic analysis process is called "Two-step speed clustering (TSC) algorithm". TSC algorithm improves the accuracy of a daily pattern clustering based on the time-series speed variation data. The experiments of the algorithm have been conducted with point detector data, installed at a Ansan city, and verified through comparison with a clustering techniques using SPSS. Our efforts in this study are expected to contribute to developing pattern-based information processing, operations management of daily recurrent congestion, improvement of daily signal optimization based on TOD plans.