• Journal of Plant Biotechnology
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• 제48권2호
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• pp.86-92
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• 2021

The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.213-221
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• 2022

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

• 한국자원식물학회지
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• 제37권3호
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• pp.225-234
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• 2024

Chinese lobelia (Lobelia chinensis Lour.) is an important medicinal plant that is used in traditional Chinese, Korean, and Japanese medicine. The goal of the current study was to develop an in vitro propagation technique for Lobelia chinensis. We have examined the effects of different media compositions on the regeneration of shoots from nodal cultures of Lobelia chinensis, including Murashige and Skoog (MS), Gamborg (B5), Schenk and Hildebrandt (SH), Woody plant (WPM), Chu (N6), and Nitsch and Nitsch (NLN) media. Similar to this, shoot regeneration was examined using MS medium of double (2.0), full (1.0), half (0.5), and quarter (0.25) strengths. The regeneration of shoots was also examined with additions of 0, 1, 3, 5, and 7% (w/v) sucrose to MS media. For axillary shoot regeneration, full-strength MS medium supplemented with 3% (w/v) sucrose was shown to be the most effective of all the evaluated factors. On this medium, nodal explants optimally regenerated 4.5 shoots per explant and subsequently shoots involved in rooting on the same medium. The regenerated plants possessed abundant phenolics, flavonoids, and DPPH, ABTS, and FRAP antioxidant activities. High performance liquid chromatographic examination (HPLC) of the regenerated plants revealed an accumulation of myricetin and catechin in higher amounts.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.151-155
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• 2000

Shoot proliferation was obtained from shoot tips and nodal explants of Vitex negundo L. on MS medium supplemented with either BAP or KIN (0.1-2.0 mg/L) alone or in combination with NAA (0.1 mg/L). The concentrations of cytokinins combined with NAA produced multiple shoots from shoot tips and nodal explants. The highest mean percentage (84.3$\pm$8.0) of shoot multiplication's were observed on nodal explants in the presence of BAP (1.5 mg/L) and NAA (0.1 mg/L) followed by shoot tips (65.0$\pm$5.0). The regenerated shootlets were rooted on MS basal medium IAA, IBA, NAA (0.1-1.5 mg/L). The maximum number of roots (51.0$\pm$2.6) was achieved on the medium containing IBA (1.0 mg/L) followed by other auxins (NAA, IAA). The regenerated plants were successfully transferred to a mixture of vermiculate and soil. About 95% of the plantlets survived when transferred to the field.

• Journal of Forest and Environmental Science
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• 제34권1호
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• pp.77-81
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• 2018

Mass production of forest medicinal plants is related to quality control of raw medicinal materials. Plant tissue culture is an important technology to produce high-quality plant materials. Numerous factors are reported to influence the success of in vitro regeneration of medicinal plants. Embryogenesis is known to be the most effective techniques and it has developed in some medicinal plant species. Various in vitro cultural condition for direct and/or indirect somatic embryogenesis systems have developed in Epimedium koreaum, Bupleurum falcatum, Paeonia lactiflora, Chrysanthemum zawadskii, Houttuynia cordata etc. In this study, we provide the present statue and information of in vitro propagation techniques that is able to apply as an efficient system for rootstock propagation system of forest medicinal plants.

• 식물조직배양학회지
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• 제26권1호
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• pp.27-30
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• 1999

복숭아 품종 백미조생, 대구보, 유명의 기내 신초 증식 및 발근에 미치는 생장조절제의 종류 및 농도의 영향을 알아보기 위하여 일련의 실험을 실시하였다. 기내 신초 대량증식에 효과적인 cytokinin은 2.0 mg/L BA처리였으며, BA와 auxin과의 혼용처리 결과 2.0mg/L BA와 1.0mg/L IAA혼용처리가 백미조생 품종의 신초 증식률에 가장 효과적이었다. 배양된 신초의 기내발근에 효과적인 auxin은 IAA였으며,그 농도는 백미조생에서는 5.0mg/L, 대구보에서는 3.0mg/L였다.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.201-207
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• 2003

Tissue culture techniques arguably are an important approach for ex situ conservation of rare and endangered plant species. However, there is utmost importance on maintaining the genetic integrity of the introduced plants especially in tree species. To examine the genetic integrity of the micropropagated plants, we randomly screened few hardened plants of Syzygium travancoricum, a critically endangered tree taxon, using Randomly Amplified Polymorphic DNA (RAPD) markers. Twenty-three random. primers were tried and twenty-five polymorphic loci were identified. The dendrogram based on the Unweighted Pair-Group Method Arithmetic Average and Nei's similarity index depicted about 97% homology between the mother plants and micropropagated plants. Further, an attempt was made to reintroduce the micropropagated plants in the wild. Over three hundred small trees could be successfully established.

• 식물조직배양학회지
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• 제21권3호
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• pp.173-176
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• 1994

작약절편을 접종하면 배지와 절편이 흑갈색으로 변하고 수 이로부터 배양이 죽기 시작하는데 배지성분 중에 이를 조장하는 물질이 있음을 발견하였다. 즉 변색의 주원인은 철분과 염화칼슘(Cacl$_2$)이었고, 절편을 괴사시키는데 가장 큰 영양을 미친 것은 NO$_3$었다. 이 결과를 토대로 피해가 비교적 적은 1/4 MS를 번식용 배지로 이용하여 식물체의 모든 부분을 배양하였을 때 조직으로부터 재생은 전혀 일어나지 않았다. 다만 줄기절편에서 이미 형성된 액아가 유식물체로 성장하기도 하였으나 건전한 것으로 계속 성장하지 못하였으며 이것을 다시 번식체로 이용할 때는 초기 배양의 절편에서 나타나는 현상과 같은 원인으로 인하여 생존하지 못하였다.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2002년도 학술발표논문집
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• pp.93-96
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• 2002

감자는 오랜 재배 역사를 가진 세계4대 식량작물로 영양가가 풍부하며 생산량이 어느 작물보다 많기 때문에 전략식량으로 많이 이용되고 있다. 최근 가공산업의 발달로 감자의 수요가 급격히 증가함에 따라 감자의 대량 생산에 관련된 많은 연구들이 수행되어 왔으며, 그 중 미세증식 기법은 단기간에 대량 생산할 수 있다는 점에서 각광을 받고 있다. (중략)

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.171-172
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• 2009