Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Rapid micropropagation of wild garlic (Allium victorialis var. platyphyllum) by the scooping method

  • Jeong, Mi Jin (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service) ;
  • Yong, Seong Hyeon (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Kim, Do Hyeon (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Park, Kwan Been (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Kim, Hak Gon (Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute) ;
  • Choi, Pil Son (Department of Medicinal Plant Resources, Nambu University) ;
  • Choi, Myung Suk (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
  • Received : 2022.07.24
  • Accepted : 2022.08.02
  • Published : 2022.09.30
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Abstract

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

Keywords

Acknowledgement

This research was funded by the Forestry Science and Technology Research and Development Project (Forest Convergence Specialist Training Project), Project No. (2020186A00-2022-AA02).

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