• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.158-162
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• 1995

The effect of carbon and nitrogen sources on the production of novel aminopeptidase M inhibitors MR-387A and B by Streptomyces sp. SL-387 has been studied. High D-glucose and ammonia concentrations (5$\%$ and 1$\%$, respectively) exerted a negative influence on the inhibitor formation. The suppressive effect of glucose on the inhibitor formation is probably caused by an effect of medium pH rather than that of cyclic AMP. To establish the optimum conditions for inhibitor overproduction, various nitrogen sources and ammonium ion-trapping agents were examined. The use of ammonia slow-releasing nitrogen sources such as soybean meal and fish meal, or ammonium ion-trapping agents such as kaoline, celite, and natural zeolite achieved the enhancement of inhibitor production. These results also indicate that inhibitor formation is affected by ammonium ion repression.

• 대한미생물학회지
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• 제35권5_6호
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• pp.372-372
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• 2000

• 한국환경과학회지
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• 제24권12호
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• pp.1705-1710
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• 2015

The aim of the present study was to determine the effects of soil microbial agent with red ginseng marc on growth of watermelon during 5 months. The three treatments were distributed in a completely randomized design with four replicates per plot. After 1 week in planting dates, the growth of watermelon (full length, stem thichness, leaf length and lead width) showed no significant difference in all treatments. During elongation stage (20 days), soil microbial agent with red ginseng marc was increased by 5% in leaf thickness (May 23) and 7~14% in leaf length (May 16 and 23) when compared to other treatments. For changes in fruit bearing thickness, there were no differences among treatments. Characteristics of watermelon in harvest season have an effect on harvest and length, stalk length, naval length, weight, sugar content and yield, except for harvest and width. In particular, yields increased with treatments with two soil microbial agent (7~12%), indicating that soil microbial agent with red ginseng marc showed higher yield than the other treatments. In conclusion, red ginseng marc-treated soil microbial agents have a positive effect on the harvest season of watermelon and can provide useful information for the selection of the functional microbial properties and the registration of microbial fertilizer.

• 생명과학회지
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• 제8권1호
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• pp.67-71
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• 1998

다량의 시료를 빠르게, 적은 격비로, 간다히 검색할수 있는 예비선별법을 찾던중, ccDNA와 sulforhodamine B를 각각 사용한 미생물배양액내의 신규항암후보물질의 탐색을 시도하였다. cccDNA법으로는 3.3%가 positive 반응을 나태내었으며, SRB assat에서는 A549와 SK-OV-3에 활성을 나타내는 4개의 발효여액을 확인한수 있었다.

• 한국미생물·생명공학회지
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• 제41권3호
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• pp.292-299
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• 2013

본 연구에서는 분자생물학적 기법인 PCR-DGGE를 사용하여 친환경 농법을 적용한 고추경작지에서 서식하는 진균의 군집 변화를 분석하고자 하였다. 먼저 토양으로부터 추출한 DNA는 DGGE 분석을 위해 진균의 universal primer인 ITS 1/4 primer set를 사용하여 nested-PCR을 수행하였으며, 증폭된 산물을 사용하여 DGGE를 수행한 결과 진균의 군집을 나타내는 band의 수는 고추 정식 전에는 3-4개에 불과했으나 고추를 정식한 후에는 전체 처리구에서 평균 15개로 조사되어 작물의 정식이 진균의 밀도 및 다양성을 증가시키는 것으로 확인되었다. 처리구 별로는 윤작과 컨소시엄 미생물제제를 동시에 적용한 고추 경작지에서 band 수가 18개로 나타나 가장 많은 것으로 조사되었다. 반면에 연작지의 화학농약 처리구에서는 band의 수가 14개로 나타나 처리구 중에서 진균의 다양성이 가장 낮은 것으로 확인되었다. 또한 식물에 질병을 일으키는 주요 병원성 진균의 DNA를 marker로 사용하여 각 처리구 별로 패턴을 비교한 결과, 연작지에서 모잘록병을 일으키는 R. solani AG-1 (IB)이 존재함을 확인할 수 있었다. 또한 염기서열 분석을 통해 우점종을 조사한 결과, 고추 정식 전에는 Paraphaeosphaeria quadriseptata, 정식 후에는 Mortierella chlamydospora, Cucurbitaria berberidis 및 Chaetomium globosum 종이 우점하고 있는 것으로 확인되었다. 처리구 간의 유사성 분석에서는 연작지의 컨소시엄 미생물제제 처리구와 윤작지의 컨소시엄 미생물제제 처리구가 유사한 것으로 나타났으며, 화학농약 처리구 역시 경작체계가 다름에도 불구하고 유사성이 있는 것으로 확인되었다.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.278-285
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• 1998

The microdilution assay recommended by NCCLS (National Committee for Clinical Laboratory Standards) is one of the standardized methods of antibiotic susceptibility test. This method has been widely used clinically to obtain MIC values of antibiotics on pathogenic microorganisms. It is more convenient, rapid and simple to test many samples than other test methods such as agar diffusion assay and broth macrodilution assay. The screening of antimicrobial agents from microbial extracts is too laborious in its process. Therefore, a number of screening methods having more simple procedure have been developed. In our laboratory, we applied microdilution assay for screening the antimicrobial agents. This assay showed dose-response results and was more sensitive than disc diffusion assay in our system. We tested 200 samples of microbial extracts originated from 100 microbial strains and selected several samples as potential candidates. In this report, we show that the microdilution assay is more convenient method in screeing of antibiotic susceptibility than those previously reported.

• 한국미생물·생명공학회지
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• 제17권3호
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• pp.219-223
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• 1989

Hydrocortisone의 $\Delta$$^1$-Dehydrogenation에 의한 prednisolone의 생산에서 소포제인 silicone oil과 neolin 302의 영향이 조사되었다. 미생물 전환방법은 pseudo-crystallofermentation 기법에 의해 수행되었다. 스테로이드 입자들은 소포제와의 hydrophobic-hydrophobic interaction에 의해 서로 응집되었다. 이러한 응집 현상에 의해 고체 기질의 용해속도와 비례하는 물질전달 면적이 감소됨으로써, 결국 생물전환속도의 감소를 유발하게 된다. Neolin은 silicone oil에 비해 전환속도에 더욱 좋지 않은 영향을 끼쳤으며, 소포제 농도가 증가할수록 전환속도는 감소하였다.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.1-7
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• 2000

To develop natural antimicrobial agents for keeping qualities of postharvested greenhouse produce the antimiocrobial actions of Polygonum cuspidatum Sieb. et Zucc. extract , which showed remarkable antimicrobial effects against microorganism causing the postharvest decay of greenhouse produce, were investigate. In the inhibitory experiment of enzymes related to energy production metabolism hexokinase activities decreased to 73% and 68% by treating with Polygonum cuspidatum Sieb. et.Zucc. extract and Eugenia caryophyllata Thumnberg extract in comparison with control, respectively. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed that microbial cell membrane was destroyed by treating with the dilute extract solution. this change of celluloar membrane permeability could be identified in the experiment that 0-nitrophenyl-${\beta}$-D-galactopyrano-side(ONPG), the artificial substrate of ${\beta}$-galactosidase, was hydrolyzed in the presence of the extract, indicating that the membrane was perturbed. The separation and identification of the most antimicrobialo substances isolated from Polygonum cuspidatum Sieb et. Zucc. extract and Eugenia caryophyllata Thunberg extract were carried out by using gas chromatography and mass spectrometry 9GC/MSD), which were identified as eugenol. As a result, the functionality of Polygonum cuspidatum Sieb. et Zucc. extract and Eugenia caryophyllata Thunberg extract as antimicrobial agents for keeping qualities of postharvested greenhouse produce may be recommended.

• 한국균학회지
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• 제49권4호
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• pp.441-454
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• 2021

Fungal diseases including anthracnose, stem rot, blight, wilting, and root rot of crops are caused by phytopathogens such as Colletotrichum species, Sclerotinia sclerotiorum, Phytophthora species, and Fusarium oxysporum and F. solani which threaten the production of chili pepper. In this study, to identify biological control agents (BCAs) of phytopathogenic fungi, potentially useful Bacillus species were isolated from the field soils. We screened out five Bacillus strains with antagonistic capacity that are efficiently inhibiting the growth of phytopathogenic fungi. Bacillus species were characterized by the production of extracellular enzymes, siderophores, and indole-3-acetic acid (IAA). Furthermore, the influence of bacterial strains on the plant growth promoting activity and seedling vigor index were assessed using Brassica juncea as a model plant. Inoculation with Bacillus subtilis SRCM 121379 significantly increased the length of B. juncea shoots and roots by 45.6% and 52.0%, respectively. Among the bacterial isolates, Bacillus subtilis SRCM 121379 showed the superior enzyme activities, antagonistic capacity and plant growth promoting effects. Based on the experimental results, Bacillus subtilis SRCM 121379 (GenBank accession no. NR027552) was finally selected as a BCA candidate.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5497-5500
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• 2012

Objective: The aim of this study was to assess the level of cancer awareness after delivering educational programs by assessing the awareness in regard to the role of microbial infectious agents in the etiology of some cancers. Methodology: Data were collected during Cancer Awareness and Educational Programs which were conducted at various places such as schools, clubs, mosques and Government health centres. This survey included 200 participants. Results: Of the persons who responded to the question, whether some viruses, bacteria, parasites and fungi, can cause cancer, 111/134 (82.8%), 105/129 (81.4%), 102/124 (82.8%) and 95/122 (77.8%), respectively, answered yes. Conclusion: The level of cancer awareness is low amongst Sudanese northern state inhabitants even after delivering an educational program.