Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Use of cccDNA Breakage Assay and Sulforhodamine B Assay for the Prescreening of Antitumor Agents from Microbial Sources

미생물 배양액으로부터 항암제의 예비선별을 위한 cccDNA Breakage 활성검정과 Assay Sulforhodamine B 활성검정의 이용

  • 이상한 (경북대학교 미생물학과) ;
  • 이동선 (경북대학교 미생물학과) ;
  • 김종국 (경북대학교 미생물학과) ;
  • 홍순덕 (경북대학교 미생물학과)
  • Published : 1998.02.01
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Abstract

In order to develop new antitumor agents from fermentation broths, we used cccDNA breakage assay abd sulforhodamine B assay for prescreening. As a result, it was shown that sample reach 3.3% when using cccDNA breakage assay. In sulforhodamine B assay, we obtained 4 acive fraction against A549 (a cell line of human lung carcinoma) and SK-OV-3 (a cell line of human adenocarcinoma). These results suggest that these assay would be a promising method for antitumor prescreening from microbial sources.

다량의 시료를 빠르게, 적은 격비로, 간다히 검색할수 있는 예비선별법을 찾던중, ccDNA와 sulforhodamine B를 각각 사용한 미생물배양액내의 신규항암후보물질의 탐색을 시도하였다. cccDNA법으로는 3.3%가 positive 반응을 나태내었으며, SRB assat에서는 A549와 SK-OV-3에 활성을 나타내는 4개의 발효여액을 확인한수 있었다.

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