The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Characterization of an Antifungal and Plant Growth-Promoting Microbe

  • Park, Se Won (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Yang, Hee-Jong (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Seo, Ji Won (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Kim, Jinwon (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Su-ji (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Ha, Gwangsu (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Ryu, Myeong Seon (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Yang, Hee Gun (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Jeong, Do-Youn (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Lee, Hyang Burm (Department of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2021.09.08
  • Accepted : 2021.12.15
  • Published : 2021.12.31
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Abstract

Fungal diseases including anthracnose, stem rot, blight, wilting, and root rot of crops are caused by phytopathogens such as Colletotrichum species, Sclerotinia sclerotiorum, Phytophthora species, and Fusarium oxysporum and F. solani which threaten the production of chili pepper. In this study, to identify biological control agents (BCAs) of phytopathogenic fungi, potentially useful Bacillus species were isolated from the field soils. We screened out five Bacillus strains with antagonistic capacity that are efficiently inhibiting the growth of phytopathogenic fungi. Bacillus species were characterized by the production of extracellular enzymes, siderophores, and indole-3-acetic acid (IAA). Furthermore, the influence of bacterial strains on the plant growth promoting activity and seedling vigor index were assessed using Brassica juncea as a model plant. Inoculation with Bacillus subtilis SRCM 121379 significantly increased the length of B. juncea shoots and roots by 45.6% and 52.0%, respectively. Among the bacterial isolates, Bacillus subtilis SRCM 121379 showed the superior enzyme activities, antagonistic capacity and plant growth promoting effects. Based on the experimental results, Bacillus subtilis SRCM 121379 (GenBank accession no. NR027552) was finally selected as a BCA candidate.

Keywords

Acknowledgement

This work was supported by a grant from the Establishment of Integrated Biobank for Agriculture, Food and Livestock Microbiome Project funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA).

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