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http://dx.doi.org/10.4489/KJM.20210043

Isolation and Characterization of an Antifungal and Plant Growth-Promoting Microbe  

Park, Se Won (Microbial Institute for Fermentation Industry (MIFI))
Yang, Hee-Jong (Microbial Institute for Fermentation Industry (MIFI))
Seo, Ji Won (Microbial Institute for Fermentation Industry (MIFI))
Kim, Jinwon (Microbial Institute for Fermentation Industry (MIFI))
Jeong, Su-ji (Microbial Institute for Fermentation Industry (MIFI))
Ha, Gwangsu (Microbial Institute for Fermentation Industry (MIFI))
Ryu, Myeong Seon (Microbial Institute for Fermentation Industry (MIFI))
Yang, Hee Gun (Microbial Institute for Fermentation Industry (MIFI))
Jeong, Do-Youn (Microbial Institute for Fermentation Industry (MIFI))
Lee, Hyang Burm (Department of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University)
Publication Information
The Korean Journal of Mycology / v.49, no.4, 2021 , pp. 441-454 More about this Journal
Abstract
Fungal diseases including anthracnose, stem rot, blight, wilting, and root rot of crops are caused by phytopathogens such as Colletotrichum species, Sclerotinia sclerotiorum, Phytophthora species, and Fusarium oxysporum and F. solani which threaten the production of chili pepper. In this study, to identify biological control agents (BCAs) of phytopathogenic fungi, potentially useful Bacillus species were isolated from the field soils. We screened out five Bacillus strains with antagonistic capacity that are efficiently inhibiting the growth of phytopathogenic fungi. Bacillus species were characterized by the production of extracellular enzymes, siderophores, and indole-3-acetic acid (IAA). Furthermore, the influence of bacterial strains on the plant growth promoting activity and seedling vigor index were assessed using Brassica juncea as a model plant. Inoculation with Bacillus subtilis SRCM 121379 significantly increased the length of B. juncea shoots and roots by 45.6% and 52.0%, respectively. Among the bacterial isolates, Bacillus subtilis SRCM 121379 showed the superior enzyme activities, antagonistic capacity and plant growth promoting effects. Based on the experimental results, Bacillus subtilis SRCM 121379 (GenBank accession no. NR027552) was finally selected as a BCA candidate.
Keywords
Antifungal activity; Bacillus subtilis; Biological control agent (BCA); Plant growth promoting bacteria; Screening;
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