• 공업화학
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• 제33권2호
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• pp.145-150
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• 2022

Microalgae are attracting attention as a resource for the production of biofuels, food nutrients, biochemicals, and bioplastics. Among a wide range of sources of the biomass, microalgae have been highlighted due to relatively easy cultivation, ability to eliminate carbon dioxide, and low culturing cost. Despite the great potential of microalgal biomass as a biological material, the complexity and relatively expensive downstream processes have inhibited the commercial use of microalgae. In this study, we reviewed recent techniques for microalgal drying for the production of microalgal based products. As drying processes comprise the largest portion of microalgae processing cost, an efficient drying technique is key to the utilization of microalgal biomass.

• ALGAE
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• 제17권2호
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• pp.117-125
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• 2002

Five microalgal species isolated from the Jeju coast and four microalgal stock strains in hatchery were cultured in order to investigate their adapation to extreme changes in environmental factors such as salinity, water temperatue, adn nutrients. In case of salinity variation, Nitzschia sp. of Bacillariophyceae, Isochrysis galbana of Haptophyceae and Tetraselmis gracilis of Prasinophyceae showed optimum growth at the low salinity of 20 and 25 psu. Amphora coffeaeformis and Chetoceros simplex of Bacillariophyceae, and Pavlova lutheri of Haptophyceae adapted well at the relatively high salinities of 30 and 35 psu. However Phaeodactylum tricornutum of Bacillariophyceae and Chlorella sp. of Chlorophyceae showed euryhaline property In case of water temperature variation, most of all the species studied wer inhibited at 10℃. C. simplex, Nitzschia sp., p. tricornutum, Chlorella sp. and T. gracilis grew well at above 20℃. A. coffeaeformis, I. galbana and P. lutheri adapted also at the high temperature of 30℃. Each microalgal strain showed different growth rates and its maximum biomass. Generally microalgal populations from the Jeju coast grow well in relatively high salinity and high water temperature. Their growth were inhibited at low water temperature, but not likely affected at low salinity. This study indicates that the microalgal populations could not be affected by abnormally low salinity phenomena, which have happened occasionally around the west Jeju coast in summer and have led macrobenthic animals to mass mortality.

• Environmental Engineering Research
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• 제23권3호
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• pp.229-241
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• 2018

Microalgae are likely to become a part of our everyday diet in the near future as they are considered to be rich in proteins, carbohydrates, and high density lipoproteins. They will play a pivotal role in the food cycle of many people around the globe. Use of microalgae in treating wastewater is also one of the disciplines which are luring researchers as this contributes to a sustainable way of exploiting resources while keeping the environment safe. In addition, microalgal biomass also has the potential to be used as a feedstock for producing biofuel, bio fertilizers, pharmaceuticals, nutraceuticals and other bio-based products. This review presents the different value-added products obtained from microalgal biomass and the applicability of these products commercially.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.630-637
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• 2018

The high rate algal ponds (HRAP) powered and mixed by a paddlewheel have been widely used for over 50 years to culture microalgae for the production of various products. Since light incidence is limited to the surface, water depth can affect microalgal growth in HRAP. To investigate the effect of water depth on microalgal growth, a mixed microalgal culture constituting three major strains of microalgae including Chlorella sp., Scenedesmus sp., and Stigeoclonium sp. (CSS), was grown at different water depths (20, 30, and 40 cm) in the HRAP, respectively. The HRAP with 20cm of water depth had about 38% higher biomass productivity per unit area ($6.16{\pm}0.33g{\cdot}m^{-2}{\cdot}d^{-1}$) and required lower nutrients and energy consumption than the other water depths. Specifically, the algal biomass of HRAP under 20cm of water depth had higher settleability through larger floc size (83.6% settleability within 5 min). These results indicate that water depth can affect the harvesting process as well as cultivation of microalgae. Therefore, we conclude that water depth is an important parameter in HRAP design for mass cultivation of microalgae.

• 환경생물
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• 제31권2호
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• pp.61-68
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• 2013

Microalgae can grow autotrophically with the supply of light, carbon dioxide and inorganic nutrients in water through photosynthesis. Generally, microalgal growth is limited by the concentrations and relative ratio of nitrogen (N) and phosphorus (P) among the nutrients in the aquatic environment. Each microalga has its specific optimum N : P ratio resulting in dominance in a particular water having similar nutrient composition. Algal bloom is an immense growth of certain microalga commonly cyanobacterium and can be sequestrated by reducing the limiting nutrient, generally P in the freshwater. Moreover, dominance of a less toxic blooming strain can be established by manipulating N : P ratio in the water. On the other hand, microalgal biomass of a certain species can be enhanced by increasing limiting nutrient and adjusting the N : P ratio to the target species. The above-mentioned eco-physiological features of microalgae can be more completely interpreted in connection with their genomic informations. Consequently, microalgal growth regulation which can be achieved on the basis of its eco-physiological and further genomic insights would be helpful not only in the control of algal bloom, but also for an increased yield of algal biomass.

• 한국미생물·생명공학회지
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• 제46권4호
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• pp.377-389
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• 2018

In this study, a novel microalgal strain, Desmodesmus sp. KAERI-NJ5, was isolated, identified, and evaluated as a candidate for biodiesel feedstock. In a preliminary study, the effects of four general microalgal growth factors, including temperature, pH, light intensity, and concentration of nitrogen source ($KNO_3$), on the microalgal photoautotrophic growth were evaluated. With the exception of light intensity, the growth factors needed to be optimized for the microalgal biomass production. Optimization was done using response surface methodology. The optimal conditions for biomass production were pH 6.54, $27.66^{\circ}C$, and 0.52 g/l $KNO_3$. The biomass production at the optimal conditions was 1.55 g/l, which correlated well with the predicted value of 1.5 g/l. The total lipid and fatty acid methyl ester contents of the cells grown at the optimal conditions were 49% and 21.2% of cell dry weight, respectively. To increase the lipid content of the biomass, microalgae were challenged by nitrogen starvation. Enhancement of total lipid and fatty acid content up to 52.02% and 49%, respectively, were observed. Lipid analysis of the nitrogen-starved cells revealed that C16 and C18 species accounted for 95.9% of the total fatty acids. Among them, palmitic acid (46.17%) and oleic acid (39.43%) dominantly constituted the algal fatty acids. These results suggest Desmodesmus sp. KAERI-NJ5 as a promising feedstock for biodiesel production.

• Environmental Engineering Research
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• 제24권2호
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• pp.271-278
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• 2019

Mass cultivation of microalgae is necessary to achieve economically feasible production of microalgal biodiesel. However, the high cost of nutrients is a major limitation. In this study, corncob extract (CCE) was used as an inorganic and organic nutrient source for the mass cultivation of Chlorella vulgaris (C. vulgaris). Chemical composition analysis of CCE revealed that it contained sufficient nutrients for mixotrophic cultivation of C. vulgaris. The highest specific grow rate of C. vulgaris was obtained at pH of 7-8, temperature of $25-30^{\circ}C$, and CCE amount of 5 g/L. In the analysis using various growth models, Luong model was found to be the most suitable empirical formula for mass cultivation of C. vulgaris using CCE. Analysis of biomass and production of triacyglycerol showed that microalgae grown in CCE medium produced more than 17.23% and 3% more unsaturated fatty acids than cells cultured in Jaworski's Medium. These results suggest that growing microalgae in CCE-supplemented medium can increase lipid production. Therefore, CCE, agricultural byproduct, has potential use for mass cultivation of microalgae.

• 미생물학회지
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• 제50권4호
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• pp.313-318
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• 2014

최근 지구온난화문제와 화석연료 자원의 부족문제로 인해 지속적이고 친환경적인 미세조류 기반 바이오디젤이 주목받고 있다. 본 연구에서는 돈분 액체비료와 농업용 복합비료가 바이오 디젤 생산을 위한 미세조류 영양원으로 활용될 가능성을 확인해 보았다. 혼합 미세조류 CSS (Chlorella spp., Scenedesmus spp., Stigeoclonium spp.) 배양을 위한 영양원은 돈분 액체비료, 돈분 액체비료와 농업용 복합비료의 혼합, 농업용 복합비료이며, 28일간 Small Scale Raceway Pond (SSRP)에서 옥외배양하였다. 그 결과 혼합 실험구의 바이오매스와 지질 생산성은 각각 0.8g/L, 5.8 mg/L/day로 가장 우수한 바이오매스량과 지질 생산성을 보여주었다. 이 연구를 통해 농업용 복합비료와 돈분 액체비료의 혼합액이 미세조류에 안정적인 영양 공급을 하는 영양원으로 활용될 가능성을 확인하였다. 또한, 미세조류 배양단가와 바이오디젤 생산단가를 낮출 가능성도 탐구하였다.

• KSBB Journal
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• 제25권2호
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• pp.109-115
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• 2010

바이오 디젤은 석유 기반의 액체 연료를 대체할 수 있는 재생 가능한 대체 에너지로서, 특히 수송 연료에 있어 경유를 대신하여 일부 사용되어 있고 수요는 계속 증가할 것으로 보인다. 앞서 살펴본 바대로, 미세 조류의 지질을 바이오 디젤의 원료로 사용하는 방안은 기술적으로는 이미 실현 가능하다. 모든 생산 과정을 최적화시키고 biorefinery 개념을 도입하여 경제성을 최대한 끌어 올리며, 광생물반응기를 좀 더 개선시켜 효율을 높임과 동시에 수요 증가에 의해 가격이 낮아지게 되면 미세 조류를 이용한 바이오 디젤 생산의 경제적 문제를 해결할 수 있을 것이다. 특히 미세 조류에서의 유전공학적, 대사공학적인 지질 합성에 관한 연구는 아직도 몇 가지 유전자를 조작해보는 초기단계에 있고 이에 대한 발전 가능성은 긍정적이므로 앞으로 많은 연구자들이 이 분야에 관심을 가진다면 미세 조류 바이오 디젤의 실용화는 한 단계 앞당겨지리라 기대한다.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.109-118
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• 2015

Open raceway ponds are cost-efficient for mass cultivation of microalgae compared with photobioreactors. Although low-cost options like wastewater as nutrient source is studied to overcome the commercialization threshold for biodiesel production from microalgae, a cost analysis on the use of wastewater and other incremental increases in productivity has not been elucidated. We determined the effect of using wastewater and wavelength filters on microalgal productivity. Experimental results were then fitted into a model, and cost analysis was performed in comparison with control raceways. Three different microalgal strains, Chlorella vulgaris AG10032, Chlorella sp. JK2, and Scenedesmus sp. JK10, were tested for nutrient removal under different light wavelengths (blue, green, red, and white) using filters in batch cultivation. Blue wavelength showed an average of 27% higher nutrient removal and at least 42% higher chemical oxygen demand removal compared with white light. Naturally, the specific growth rate of microalgae cultivated under blue wavelength was on average 10.8% higher than white wavelength. Similarly, lipid productivity was highest in blue wavelength, at least 46.8% higher than white wavelength, whereas FAME composition revealed a mild increase in oleic and palmitic acid levels. Cost analysis reveals that raceways treating wastewater and using monochromatic wavelength would decrease costs from 2.71 to 0.73 $/kg biomass. We prove that increasing both biomass and lipid productivity is possible through cost-effective approaches, thereby accelerating the commercialization of low-value products from microalgae, like biodiesel.