• 제목/요약/키워드: miR-25

검색결과 441건 처리시간 0.027초

반도체 패키징용 금-코팅된 은 와이어의 부식특성 (Corrosion Characteristics of Gold-Coated Silver Wire for Semiconductor Packaging)

  • 홍원식;김미송;김상엽;전성민;문정탁;김영식
    • Corrosion Science and Technology
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    • 제20권5호
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    • pp.289-294
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    • 2021
  • In this study, after measuring polarization characteristics of 97.3 wt% Ag, Au-Coated 97.3 wt% Ag (ACA) and 100 wt% Au wires in 1 wt% H2SO4 and 1 wt% HCl electrolytes at 25 ℃, corrosion rate and corrosion characteristics were comparatively analyzed. Comparing corrosion potential (ECORR) values in sulfuric acid solution, ACA wire had more than six times higher ECORR value than Au wire. Thus, it seems possible to use a broad applied voltage range of bonding wire for semiconductor packaging which ACA wire could be substituted for the Au wire. However, since the ECORR value of ACA wire was three times lower than that of the Au wire in a hydrochloric acid solution, it was judged that the use range of the applied voltage and current of the bonding wire should be considered. In hydrochloric acid solution, 97.3 wt% Ag wire showed the highest corrosion rate, while ACA and Au showed similar corrosion rates. Additionally, in the case of sulfuric acid solution, all three types showed lower corrosion rates than those under the hydrochloric acid solution environment. The corrosion rate was higher in the order of 97.3 wt% Ag > ACA > 100 wt% Au wires.

닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이 (Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse)

  • 조재근;손규희;김경희;김정미;박대현;이정우
    • 한국동물위생학회지
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    • 제42권3호
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    • pp.153-159
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    • 2019
  • The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

뷰티전공과 예체능전공 대학생의 COVID-19에 대한 지식, 태도, 예방실천행위 (Knowledge, attitude, and preventive action on COVID-19 infectious disease of cosmetology major and arts and physical education college students)

  • 안미정;박선주
    • 한국의상디자인학회지
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    • 제23권4호
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    • pp.73-80
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    • 2021
  • This study is a descriptive cross-sectional study to confirm the knowledge, attitudes, and preventive practice behaviors of university students concerning COVID-19 and to identify the factors that influence preventive practice behaviors. The number of study subjects was calculated using G*power program 3.19. The data collected from the study were analyzed using SPSS/WIN version 25. The subject's characteristics, knowledge, attitude, and preventive practices against COVID-19 were analyzed with descriptive statistics, and the difference in knowledge, attitude, and prevention practices against COVID-19 according to the subject's characteristics was evaluated using an independent t-test, ANOVA, and post-hoc test was conducted using a Scheffé test. Correlations of knowledge, attitude, and prevention practices against COVID-19 were analyzed using Pearson Correlation coefficients, and factors affecting COVID-19 prevention practices were analyzed using stepwise multiple regression. The number of subjects analyzed in the study was 232. The general characteristics of the study subjects were 78 males (33.7 %) and 154 females (66.3 %). The subject's knowledge score about COVID-19 was 85.13±6.22 out of 100 points, and the attitude score toward COVID-19 was 12.47±1.31 out of 14 points. The prevention practice score for COVID-19 was 29.36±3.42 out of 32 points. As a result of the study, knowledge about COVID-19 was found to have a significant positive correlation with attitude (r=.34, p<.001); COVID-19 prevention practice behavior was found to have a significant positive correlation with knowledge (r=.54, p<.001), and attitude (r=.62, p<.001). The main factors influencing the COVID-19 prevention practice behavior were knowledge, attitude, and major (Cosmetology major).

중환자실 간호사의 의도적 반추, 자기 노출, 사회적 지지가 외상 후 성장에 미치는 영향 (Post-traumatic Growth and it's associations with Deliberate Rumination, Self-disclosure, and Social Support among Intensive Care Unit Nurses)

  • 민새미;김희준;김춘자;안정아
    • 중환자간호학회지
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    • 제15권2호
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    • pp.50-63
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    • 2022
  • Purpose : This study aimed to identify intensive care units (ICU) nurses' experience of traumatic events, deliberate rumination, self-disclosure, social support, and post-traumatic growth (PTG), and to explore relationships among the variables. Methods : Participants were 157 nurses who have provided direct patient care for six months or more in ICUs at a university hospital. Collected data were analyzed using descriptive statistics, independent t-tests, one-way ANOVAs, Pearson correlations, and multiple linear regressions using the SPSS/WIN version 23.0. Results : The PTG was found to be significantly associated with deliberate rumination (r=0.36, p<.001), self-disclosure (r=0.39, p<.001), and social support (r=0.54, p<.001). Factors that affect PTG significantly were found in the order of social support (𝛽=0.40, p<.001), self-disclosure (𝛽=0.25, p<.001), and deliberate rumination (𝛽=0.24, p<.001). The final regression model explained 40.1% of the variance of PTG (F=26.33, p<.001). Conclusion : The influencing factors identified in this study on PTG, including social support, self-disclosure, and deliberate rumination should be included in programs to promote PTG for ICU nurses who may experience traumatic events repeatedly.

Comparison of One-Tube Nested-PCR and PCR-Reverse Blot Hybridization Assays for Discrimination of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Infection in FFPE tissues

  • Park, Sung-Bae;Park, Heechul;Bae, Jinyoung;Lee, Jiyoung;Kim, Ji-Hoi;Kang, Mi Ran;Lee, Dongsup;Park, Ji Young;Chang, Hee-Kyung;Kim, Sunghyun
    • 대한의생명과학회지
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    • 제25권4호
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    • pp.426-430
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    • 2019
  • Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.

브루셀라병 혈청검사 양성 수소와 12개월령 이하 소에서의 균 분리 및 동정 (Isolation of Brucella spp from sere-positive native bulls and calves below twelve months old)

  • 류재윤;변정운;이희영;이용창;이종진;송영각;남향미
    • 한국동물위생학회지
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    • 제30권3호
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    • pp.375-384
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    • 2007
  • This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

하워드-돌먼 입체검사를 이용한 20대 성인의 동적 입체시 평가 (Assessment of Dynamic Stereoacuity of Adults in their 20s' with Howard-Dolman Test)

  • 심현석;최선미;김영청
    • 한국안광학회지
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    • 제20권1호
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    • pp.61-66
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    • 2015
  • 목적: 본 연구에서는 하워드-돌먼 입체검사(Howard-Dolman test, HDTEST, Bernell, U.S.A)를 이용하여 20대 성인의 동적 입체시를 측정하여 남녀 간의 동적 입체시를 비교하고, 동공 간거리(PD; pupillary distance)와 좌우안의 구면굴절력의 차이로 인한 굴절부등과 동적 입체시의 상관관계를 분석해 보고자 하였다. 방법: 평균연령이 $22.68{\pm}0.50$(20~29)세인 20대 성인 63명(남자 30명, 여자 33명)을 대상으로 실험을 실시하였다. 굴절이상자는 완전교정을 한 후에 Howard-Dolman test(H-D Test)를 이용하여 2.5 m 검사거리에서 동적 입체시를 각각 5회 측정하였다. 결과: 전체 대상자의 동적 입체시의 평균은 $28.44{\pm}25.03$초였고, 이들 중 남자는 $28.23{\pm}23.34$초, 여자는 $28.63{\pm}26.83$초였다. PD 범위를 기준으로 한 동적 입체시에 있어서 PD가 59.80 mm이하 범위에서는 $33.87{\pm}18.53$초, 59.81~66.15 mm의 범위에서는 $26.24{\pm}25.26$초, 66.15 mm를 초과하는 범위에서는 $34.60{\pm}25.65$초로 나타났고, 유의한 차이는 보이지 않았다(P=0.73, r=0.03). 굴절부등의 차이를 기준으로 할 때 동적 입체시는 굴절부등이 1 D미만인 경우 $26.81{\pm}24.86$초, 1 D이상인 경우는 $1.45{\pm}24.18$초였고 유의한 차이는 없었다(P=0.15, r=0.15). 결론: H-D test를 이용해 20대 성인의 동적 입체시를 평가해 보았을 때, 남녀 간에 큰 차이가 없고, PD와 굴절부등은 동적 입체시에 큰 영향을 주지 않는 것으로 나타났다.

방문객 유형별 이용객수에 대한 규범적 평가기준, 혼잡지각과 치유경험방해 지각: '장성 편백치유의 숲'의 '치유필드'를 대상으로 (Normative Standards on Number of Users, Perceived Conflict, and Perceived Interference of Healing Experience by Types of Visitors at 'Healing Field' of 'Jangseong Pyunbaek Healing Forest')

  • 김상오;김상미
    • 한국환경생태학회지
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    • 제32권2호
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    • pp.231-243
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    • 2018
  • 본 연구는 방문객 유형별로 장성 편백치유의 숲(Jangseong Pyunbaek Healing Forest: JPHF) 내 '치유필드'의 이용객수에 대한 지각(perceived number of users: PNU)과 이용객수에 대한 평가기준(evaluative standards)을 설정, 비교하기 위해 시행되었다. 2017년 9월 중 JPHF 내 '치유필드' 이용객을 대상으로 편의표본추출 방법을 통해 선정된 158명의 표본으로부터 설문지기법을 이용하여 자료를 수집하였다. 치유필드 방문객 중 휴양객(일반 휴양을 목적으로 한 방문객)은 49.3%, 치유객(건강 및 치유를 목적으로 한 방문객) 15.3%, 휴치객(휴양과 치유를 동일정도로 추구하는 방문객) 35.3%로 구성되어 있었다. 응답자가 지각하는 치유필드 이용객수는 전체적으로는 평균 23.8명(중앙값: 20.0명)으로 나타났으며, 방문객 유형별 평균범위는 21.1-27.8명(중앙값: 20.0명 동일)으로 조사되었다. 응답자의 PNU는 혼잡지각(perceived crowding: PC)(r=0.23) 및 치유경험 방해 지각(perceived interference of healing experience: PIHE) (r=0.20)과 낮은 상관관계를 나타냈다. 방문객 유형 간에 치유필드 적정이용객수(optimal number of users: ONU)는 차이가 없었다. 전체적으로 적정이용객수는 평균 28.9명(중앙값: 25.0명)으로 나타났으며 방문객 유형별 적정이용객수 범위는 평균 25.4-31.4명(중앙값: 25.0-27.5명)으로 분석되었다. 또한 최대 허용가능 이용객수(maximum acceptable number of users: MANU)는 전체적으로 38.1명으로 나타났으며, 방문객 유형별로는 휴양객 36.9명, 치유객 32.0명, 휴치객 38.0명으로 각각 조사되었다. 방문객 유형별로는 MANU에 차이가 없었다. 응답자의 PC는 평균 2.73(SD: 3.26, 9점 척도), PIHE는 평균 3.26(SD: 1.77, 9점 척도)으로 나타났으며 방문객 유형별로는 휴치객이 휴양객에 비해 통계적으로 높은 PC와 PIHE를 보였다. PNU가 ONU를 초과한 그룹은 그렇지 않은 그룹에 비해 높은 PC와 PIHE를 나타냈다. 연구결과를 토대로 관리적 관점에서의 시사점을 제시하였으며 본 연구의 한계점과 추후 연구에 대하여 토의하였다.

응급구조(학)과 학생의 취업진로선택 및 취업준비 현황 (Career Choice and Employment Preparation Condition for EMT Students)

  • 박소미;최은숙;김미숙;이경열
    • 한국응급구조학회지
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    • 제15권1호
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    • pp.65-78
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    • 2011
  • Purpose : We investigated the career choice and the employment preparation of emergency medical technology (EMT) students. The results of study will provide the base information about the consciousness for career choice and employment of the EMT students. Method : We conducted 1,586 surveys from 665 students at six four-year colleges and 921 students at seven three-year colleges, from Aug. 27th to Oct. 31st in 2010. The result was analyzed by SPSS 18.0 using description statistics, t-test, ANOVA, Scheffe and Pearson correlation coefficient. Result : The general characteristics showed male 50.2% and female 49.8%, and first grade 34.6%, second grade 27.4%, third grade 28.2%, and fourth grade 9.8%. The 53.9% of students had experienced the hospital training and the 44.8% did the fire station training. For the choice of job, there were the 61.8% student who hope to enter the employ in fire station or public service, while 25.7% students hope to be employed in hospitals. The reason for a wish job were 'majors-related job' in 18.2% students, 'employment stability' in 17.2% and 'hit on the aptitude and talent' in 16.7%, 'appropriate salaries' in 15.9%. In the questionnaire of the University what to assist you for job preparation, they chose the 'support for get major related license', 'support for officer exam preparation' and 'support of foreign language study' in 34.6%, 16.8% and 16.6%, respectively. For achieved licence, they had a driver's license in 53.9%, BLS (basic life support) provider in 20.3%, life guard in 16.2% and scuba in 8.8%. The statistically significant correlation was identified between the awareness of University that provides job information and one's career awareness (r= .203, p<.000), the awareness that University provide job information and one's confidence in employment (r= .236, p<.000), and one's career awareness and one's confidence in employment (r = .356, p = .000). Conclusion : The most of EMT Students are well conscious of the their future employment and has prepared career to be employed their preference job.

랫드와 HeLaTRE Cell에서의 Alachlor에 의한 갑상선 호르몬성 영향 연구 (Thyroid Hormone-like Activity of Alachlor as R Endocrine Disruptor in Rats and HeLaTRE Cell Culture)

  • 유아선;정미혜;박경훈;김병석;김진배;권오경
    • 농약과학회지
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    • 제12권3호
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    • pp.207-214
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    • 2008
  • 내분비장애 추정물질의 분류를 위해 많은 시험법이 연구되고 있는데 추후 내분비장애 추정물질로 분류된 기등록 농약에 대한 자료요구 또는 신규 등록농약에 대한 등록기준의 추가 등을 고려하여 OECD와 EPA에서 권장하는 시험법을 확립하고자 본 연구를 수행하였다. 시험약제를 30일간 경구 투여하여 조사한 결과, alachlor 투여 수컷에서 25 mg/kg/day, 50 mg/kg/day에서 고환과 갑성선의 중량이 증가하였다. Alachlor 투여 암컷에서는 질의 중량이 25, 50 mg/kg/day에서 감소하였고 25 mg/kg/day에서 갑상선의 중량이 감소하였다. Alachlor 투여 암컷 25, 50 mg/kg/day에서 주요 갑상선 호르몬인 T4와 성호르몬 testosterone이 감소하였다. 따라서 pubertal assay 결과 alachlor는 갑상선 호르몬성 영향이 의심되었다. 시험세포를 이용한 시험 결과, 시험약제를 1 nM에서 1000 nM까지 처리하였을 때 음성대조군과 비교하여 alachlor는 100-134%의 갑상선 호르몬성 영향을 나타내었다. 따라서 세포를 이용한 시험에서는 alachlor에 의한 갑상선 호르몬성 영향이 나타나는 것으로 판단되었다. 항갑상선 호르몬성 영향 시험에서는 시험약제 100 nM과 T4의 혼합 처리시 alachlor는 항갑상선 호르몬성 영향은 나타나지 않았다.