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Thyroid Hormone-like Activity of Alachlor as R Endocrine Disruptor in Rats and HeLaTRE Cell Culture  

You, Are-Sun (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Jeong, Mi-Hye (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Paik, Kyung-Hun (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Kim, Byung-Seok (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Kim, Jin-Bae (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Kwon, Oh-Kyung (Division of Hazardous Substances, Department of Crop Life Safety, National Institute of Agricultural Science & Technology)
Publication Information
The Korean Journal of Pesticide Science / v.12, no.3, 2008 , pp. 207-214 More about this Journal
Abstract
This study was designed to investigate the suitability of the pubertal assay and the enhanced TG 407 as methods for detection of endocrine-mediated effects, especially thyroid function. Male and female Sprague-Dawley rats were gavaged daily with 0, 12.5, 25, 50 mg/kg alachlor in corn oil during 30 days. The effects of alachlor on thyroid gland, the genital organs and thyroid hormone were measured in male and female rats. Dose of alachlor 25, 50 mg/kg/day increased relative weight of testis and thyroid gland in exposed male rats and decreased relative weight of vagina in exposed female rats. Relative weight of thyroid gland was decreased in alachlor 25 mg/kg/day exposed female rats. Dose of alachlor 25, 50 mg/kg/day decreased plasma T4 and testosterone in female rats. Another purpose of this study was to investigate the effects of endocrine disruptors as like thyroid hormone in vitro. Luciferase activity was measured to dectect reaction of test chemicals and thyroid hormone response elements in HeLaTRE cell. Dose of alachlor 1 nM-1000 nM increased 100-134% luciferase activity compared with control.
Keywords
Alachlor; Thyroid hormone; Endocrine disruptor;
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