• Title/Summary/Keyword: miR-25

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Corrosion Characteristics of Gold-Coated Silver Wire for Semiconductor Packaging (반도체 패키징용 금-코팅된 은 와이어의 부식특성)

  • Hong, Won Sik;Kim, Mi-Song;Kim, Sang Yeop;Jeon, Sung Min;Moon, Jeong Tak;Kim, Youngsik
    • Corrosion Science and Technology
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    • v.20 no.5
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    • pp.289-294
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    • 2021
  • In this study, after measuring polarization characteristics of 97.3 wt% Ag, Au-Coated 97.3 wt% Ag (ACA) and 100 wt% Au wires in 1 wt% H2SO4 and 1 wt% HCl electrolytes at 25 ℃, corrosion rate and corrosion characteristics were comparatively analyzed. Comparing corrosion potential (ECORR) values in sulfuric acid solution, ACA wire had more than six times higher ECORR value than Au wire. Thus, it seems possible to use a broad applied voltage range of bonding wire for semiconductor packaging which ACA wire could be substituted for the Au wire. However, since the ECORR value of ACA wire was three times lower than that of the Au wire in a hydrochloric acid solution, it was judged that the use range of the applied voltage and current of the bonding wire should be considered. In hydrochloric acid solution, 97.3 wt% Ag wire showed the highest corrosion rate, while ACA and Au showed similar corrosion rates. Additionally, in the case of sulfuric acid solution, all three types showed lower corrosion rates than those under the hydrochloric acid solution environment. The corrosion rate was higher in the order of 97.3 wt% Ag > ACA > 100 wt% Au wires.

Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse (닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이)

  • Cho, Jae-Keun;Son, Kyu-Hee;Kim, Kyung-Hee;Kim, Jeong-Mi;Park, Dae-Hyun;Lee, Jung-Woo
    • Korean Journal of Veterinary Service
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    • v.42 no.3
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    • pp.153-159
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    • 2019
  • The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

Knowledge, attitude, and preventive action on COVID-19 infectious disease of cosmetology major and arts and physical education college students (뷰티전공과 예체능전공 대학생의 COVID-19에 대한 지식, 태도, 예방실천행위)

  • Ahn, Mi Jeung;Park, Sun Ju
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.4
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    • pp.73-80
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    • 2021
  • This study is a descriptive cross-sectional study to confirm the knowledge, attitudes, and preventive practice behaviors of university students concerning COVID-19 and to identify the factors that influence preventive practice behaviors. The number of study subjects was calculated using G*power program 3.19. The data collected from the study were analyzed using SPSS/WIN version 25. The subject's characteristics, knowledge, attitude, and preventive practices against COVID-19 were analyzed with descriptive statistics, and the difference in knowledge, attitude, and prevention practices against COVID-19 according to the subject's characteristics was evaluated using an independent t-test, ANOVA, and post-hoc test was conducted using a Scheffé test. Correlations of knowledge, attitude, and prevention practices against COVID-19 were analyzed using Pearson Correlation coefficients, and factors affecting COVID-19 prevention practices were analyzed using stepwise multiple regression. The number of subjects analyzed in the study was 232. The general characteristics of the study subjects were 78 males (33.7 %) and 154 females (66.3 %). The subject's knowledge score about COVID-19 was 85.13±6.22 out of 100 points, and the attitude score toward COVID-19 was 12.47±1.31 out of 14 points. The prevention practice score for COVID-19 was 29.36±3.42 out of 32 points. As a result of the study, knowledge about COVID-19 was found to have a significant positive correlation with attitude (r=.34, p<.001); COVID-19 prevention practice behavior was found to have a significant positive correlation with knowledge (r=.54, p<.001), and attitude (r=.62, p<.001). The main factors influencing the COVID-19 prevention practice behavior were knowledge, attitude, and major (Cosmetology major).

Post-traumatic Growth and it's associations with Deliberate Rumination, Self-disclosure, and Social Support among Intensive Care Unit Nurses (중환자실 간호사의 의도적 반추, 자기 노출, 사회적 지지가 외상 후 성장에 미치는 영향)

  • Min, Sae Mi;Kim, Hee Jun;Kim, Chun-Ja;Ahn, Jeong-Ah
    • Journal of Korean Critical Care Nursing
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    • v.15 no.2
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    • pp.50-63
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    • 2022
  • Purpose : This study aimed to identify intensive care units (ICU) nurses' experience of traumatic events, deliberate rumination, self-disclosure, social support, and post-traumatic growth (PTG), and to explore relationships among the variables. Methods : Participants were 157 nurses who have provided direct patient care for six months or more in ICUs at a university hospital. Collected data were analyzed using descriptive statistics, independent t-tests, one-way ANOVAs, Pearson correlations, and multiple linear regressions using the SPSS/WIN version 23.0. Results : The PTG was found to be significantly associated with deliberate rumination (r=0.36, p<.001), self-disclosure (r=0.39, p<.001), and social support (r=0.54, p<.001). Factors that affect PTG significantly were found in the order of social support (𝛽=0.40, p<.001), self-disclosure (𝛽=0.25, p<.001), and deliberate rumination (𝛽=0.24, p<.001). The final regression model explained 40.1% of the variance of PTG (F=26.33, p<.001). Conclusion : The influencing factors identified in this study on PTG, including social support, self-disclosure, and deliberate rumination should be included in programs to promote PTG for ICU nurses who may experience traumatic events repeatedly.

Comparison of One-Tube Nested-PCR and PCR-Reverse Blot Hybridization Assays for Discrimination of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Infection in FFPE tissues

  • Park, Sung-Bae;Park, Heechul;Bae, Jinyoung;Lee, Jiyoung;Kim, Ji-Hoi;Kang, Mi Ran;Lee, Dongsup;Park, Ji Young;Chang, Hee-Kyung;Kim, Sunghyun
    • Biomedical Science Letters
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    • v.25 no.4
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    • pp.426-430
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    • 2019
  • Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.

Isolation of Brucella spp from sere-positive native bulls and calves below twelve months old (브루셀라병 혈청검사 양성 수소와 12개월령 이하 소에서의 균 분리 및 동정)

  • Ryu, Jae-Yun;Bun, Jung-Hyun;Lee, Hee-Young;Lee, Yong-Chang;Lee, Jong-Jin;Song, Young-Gak;Nam, Hyang-Mi
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.375-384
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    • 2007
  • This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

Assessment of Dynamic Stereoacuity of Adults in their 20s' with Howard-Dolman Test (하워드-돌먼 입체검사를 이용한 20대 성인의 동적 입체시 평가)

  • Shim, Hyun-Suk;Choi, Sun-Mi;Kim, Young-Cheong
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.1
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    • pp.61-66
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    • 2015
  • Purpose: In this study, dynamic stereoacuity of 20s' adults were measured by using the Howard-Dolman test(H-D TEST, Bernell, U.S.A), and compared of male and female. And the correlation between dynamic stereoacuity and PD(pupillary distance), and between dynamic stereoacuity and anisometropia caused by difference in the spherical refractive power of the left and right eyes were analyzed. Methods: The mean age of $22.68{\pm}0.50$(20~29)years old, 20s' 63 adults (30 male, 33 female) were conducted for this experiments. After the full correction of subject's refractive error, dynamic stereoacuity was measured 5 times for 1 subject at 2.5 m distance using the H-D test. at 2.5 distance. Results: The mean of dynamic stereoacuity was $28.44{\pm}25.03$ sec of arc for total subjects, $28.23{\pm}23.34$ sec of arc for male, and $28.63{\pm}26.83$ sec of arc for female. In the dynamic stereoacuity classified by the range of inter-pupil distance (IPD), the dynamic stereoacuity was $33.87{\pm}18.53$ sec for the IPD being under 59.80 mm, $26.24{\pm}25.26$ sec of arc for 59.81~66.15 mm, $34.60{\pm}25.65$ sec of arc for over 66.15 mm. However, there were no significant differences between 3 groups (P=0.73, r=0.03). In dynamic stereoacuity classified by the refractive error difference between two eyes, dynamic stereoacuity was $26.81{\pm}24.86$ sec of arc for the under 1 D, $41.45{\pm}24.18$ sec of arc for over 1 D, and there was no significant difference between two groups (P=0.15, r=0.15). Conclusions: Dynamic stereacuity by the H-D test in 20s adults showed that there was no significant differences between male and female, and PD and anisometropia did not have a significant impact upon the dynamic stereoacuity.

Normative Standards on Number of Users, Perceived Conflict, and Perceived Interference of Healing Experience by Types of Visitors at 'Healing Field' of 'Jangseong Pyunbaek Healing Forest' (방문객 유형별 이용객수에 대한 규범적 평가기준, 혼잡지각과 치유경험방해 지각: '장성 편백치유의 숲'의 '치유필드'를 대상으로)

  • Kim, Sang-Oh;Kim, Sang-Mi
    • Korean Journal of Environment and Ecology
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    • v.32 no.2
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    • pp.231-243
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    • 2018
  • This study was conducted to determine and compare the perceived number of users (PNU) and evaluative standards for each type of visitors to "Healing Field" of Jangseong Pyunbaek Healing Forest (JPHF). Data were collected from 158 samples selected by convenient sampling method from the users of "Healing Field" in JPHF during September 2017. Visitors to "Healing Field" consisted of 49.3% for recreation (VR), 15.3% for healing (VH), and 35.3% for both recreation and healing (VRH). Respondents' average PNU was 23.8 (median: 20.0), and the range of average PNU by visitor type was 21.1-27.8 (median: 20.0). Respondents' PNU showed low correlations with perceived crowding (PC) (r=0.23) and perceived interference of healing experience (PIHE) (r=0.20). There were no differences in the optimal number of users (ONU) of "Healing Field" among the types of visitors. The overall average ONU was 28.9(median: 25.0), and the range of average ONU by types of visitors was 25.4-31.4 (median: 25.0-27.5). The overall maximum acceptable number of users (MANU) was 38.1, and MANU for VR, VH, and VRH was 36.9, 32.0, and 38.0, respectively. Respondents' mean PC was 2.73 (SD: 3.26, 9-point scale) while mean PIHE was 3.26 (SD: 1.77, 9-point scale). The VRH group showed a higher PC and PIHE than the VR group. The group with greater PNU than ONU showed higher PC and PIHE than the other groups. Based on the results, this paper presented some managerial implications, discussed the limitations of the study, and suggested the further studies.

Career Choice and Employment Preparation Condition for EMT Students (응급구조(학)과 학생의 취업진로선택 및 취업준비 현황)

  • Park, So-Mi;Choi, Eun-Sook;Kim, Mi-Sook;Lee, Kyoung-Youl
    • The Korean Journal of Emergency Medical Services
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    • v.15 no.1
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    • pp.65-78
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    • 2011
  • Purpose : We investigated the career choice and the employment preparation of emergency medical technology (EMT) students. The results of study will provide the base information about the consciousness for career choice and employment of the EMT students. Method : We conducted 1,586 surveys from 665 students at six four-year colleges and 921 students at seven three-year colleges, from Aug. 27th to Oct. 31st in 2010. The result was analyzed by SPSS 18.0 using description statistics, t-test, ANOVA, Scheffe and Pearson correlation coefficient. Result : The general characteristics showed male 50.2% and female 49.8%, and first grade 34.6%, second grade 27.4%, third grade 28.2%, and fourth grade 9.8%. The 53.9% of students had experienced the hospital training and the 44.8% did the fire station training. For the choice of job, there were the 61.8% student who hope to enter the employ in fire station or public service, while 25.7% students hope to be employed in hospitals. The reason for a wish job were 'majors-related job' in 18.2% students, 'employment stability' in 17.2% and 'hit on the aptitude and talent' in 16.7%, 'appropriate salaries' in 15.9%. In the questionnaire of the University what to assist you for job preparation, they chose the 'support for get major related license', 'support for officer exam preparation' and 'support of foreign language study' in 34.6%, 16.8% and 16.6%, respectively. For achieved licence, they had a driver's license in 53.9%, BLS (basic life support) provider in 20.3%, life guard in 16.2% and scuba in 8.8%. The statistically significant correlation was identified between the awareness of University that provides job information and one's career awareness (r= .203, p<.000), the awareness that University provide job information and one's confidence in employment (r= .236, p<.000), and one's career awareness and one's confidence in employment (r = .356, p = .000). Conclusion : The most of EMT Students are well conscious of the their future employment and has prepared career to be employed their preference job.

Thyroid Hormone-like Activity of Alachlor as R Endocrine Disruptor in Rats and HeLaTRE Cell Culture (랫드와 HeLaTRE Cell에서의 Alachlor에 의한 갑상선 호르몬성 영향 연구)

  • You, Are-Sun;Jeong, Mi-Hye;Paik, Kyung-Hun;Kim, Byung-Seok;Kim, Jin-Bae;Kwon, Oh-Kyung
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.207-214
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    • 2008
  • This study was designed to investigate the suitability of the pubertal assay and the enhanced TG 407 as methods for detection of endocrine-mediated effects, especially thyroid function. Male and female Sprague-Dawley rats were gavaged daily with 0, 12.5, 25, 50 mg/kg alachlor in corn oil during 30 days. The effects of alachlor on thyroid gland, the genital organs and thyroid hormone were measured in male and female rats. Dose of alachlor 25, 50 mg/kg/day increased relative weight of testis and thyroid gland in exposed male rats and decreased relative weight of vagina in exposed female rats. Relative weight of thyroid gland was decreased in alachlor 25 mg/kg/day exposed female rats. Dose of alachlor 25, 50 mg/kg/day decreased plasma T4 and testosterone in female rats. Another purpose of this study was to investigate the effects of endocrine disruptors as like thyroid hormone in vitro. Luciferase activity was measured to dectect reaction of test chemicals and thyroid hormone response elements in HeLaTRE cell. Dose of alachlor 1 nM-1000 nM increased 100-134% luciferase activity compared with control.