• Title/Summary/Keyword: miR-25

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Effects of Whey Protein Hydrolysates on Lipid Profiles and Appetite-Related Hormones in Rats Fed High Fat Diet (고지방식이를 섭취한 흰쥐에서 유청단백질 가수분해물의 섭취가 지질 농도 및 식욕 관련 호르몬에 미치는 영향)

  • Park, Jung-Yoon;Park, Mi-Na;Choi, You-Young;Yun, Sung-Seob;Chun, Ho-Nam;Lee, Yeon-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.4
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    • pp.428-436
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    • 2008
  • This study was carried out to compare the effects of whey protein concentrate, its hydrolysates and macropeptide fractions obtained from papain treatment of whey protein on lipid levels and appetite-related hormones in obesity model rats induced by high fat diet. Four week-old male Sprague-Dawley rats were fed high fat (18% w/w) and low protein (10% w/w) diet for 4 weeks and then divided into four groups (n=8/group). Rats were fed high fat diets containing various nitrogen sources; 10% whey protein concentrate (10WPC), 25% whey protein concentrate (25WPC), 25% whey protein hydrolysates (25WH), and 25% whey macropeptide fractions (25WP, MW$\geq$10,000), respectively for 6 weeks. There were no significant differences in body weight gain and food intake among groups. A significant decrease of total lipid, triglyceride in serum was observed in 25WH and 25WP groups. Total lipid and triglyceride contents of the liver were significantly decreased in 25WPC, 25WH and 25WP groups compared with 10WPC group. However, in the liver, there were no differences in the contents of total lipid and triglyceride among 25WPC, 25WH and 25WP groups. The daily amounts of feces were significantly increased in 25WH and 25WP groups and the excretion of total lipid and triglyceride were significantly increased in 25WH group. Serum glucose and insulin concentration were significantly decreased in 25WH group. The concentration of serum ghrelin was significantly decreased in the 25WPC, 25WH and 25WP groups compared with 10WPC group. However, there was no significant difference in the concentration of serum leptin among groups. These results suggest that whey protein hydrolysates and macropeptide fractions may show beneficial effects on the lipid profile in serum and liver, appetite regulation and insulin resistance in obesity model rats induced by high fat diet.

Development of Resilience Scale for Nurses (간호사 극복력 측정도구 개발)

  • Park, Mi Mi;Park, Jee-Won
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.23 no.1
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    • pp.32-41
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    • 2016
  • Purpose: This study was done to develop a measure resilience in nurses. Method: Forty preliminary items were extracted from a view of the literature on concept analysis and scale development, and from in-depth interviews with nurses. These items were examined for content validity, reliability and validity. The scale was verified with 496 nurses working in three advanced general hospitals located in Seoul and Gyeonggido. Results: Factor analysis resulted in the creation of the final scale, which consisted of 30 items that were grouped into 5 factors: dispositional pattern, relational pattern, situational pattern, philosophical pattern and professional pattern. The explanatory variance was 56.25%. The reliability of the scale was Cronbach's ${\alpha}=.95$. Correlation of the scale with the Korean version of the Connor-Davidson Resilience Scale (K-CD-RISC), established its construct and concurrent validity (r=.74, p<.001). Conclusion: The resilience scale for nurses was found to have reliability and validity, and as developed in this study reflects nurses' disposition and was confirmed as a basis for developing and evaluating programs to increase nurses' resilience.

Internet Obsession and Parent-Child Attachment in 5th and 6th Grade Students (초등학교 5, 6학년생의 인터넷 중독과 부모-자녀 애착)

  • Park, Jin-Young;Kim, Nam-Hye;Kim, Young-Shin;Lee, So-Hye;Jung, Mi-Ran;Jung, Soo-Kyung;Hwang, Yu-Mi;Bang, Kyung-Sook
    • Korean Parent-Child Health Journal
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    • v.12 no.1
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    • pp.25-36
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    • 2009
  • Purpose: The purpose of this study was to explore the patterns of internet usage among 5th and 6th grade elementary students, and analyze the correlation between the internet obsessive disorder and parent-child attachment. Method: A total of 303 elementary students in Seoul, Suwon, Ulsan, and Kwangjoo area participated in this study, and responded to self-administrated questionnaires. Results: With regard to the internet addiction, 2.7% of participants were classified as being in a high risk group, and 5.3% were classified as a potential risk group with boys at greater risk than girls, and 6th graders showing higher internet addiction scores than 5th graders. There were significant negative correlations between internet addiction and father-child attachment (r=-.122, p=.040), and mother-child attachment (r=-.154, p=.009). Conclusion: To prevent internet addiction in elementary school students, promoting parent-child interaction needs to be emphasized. More studies for finding affecting variables on internet addiction would be necessary, and different preventive approaches according to gender and grade should be introduced.

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Knowledge, Confidence, and Learning Needs Regarding Advance Directives among Hospital Nurses (상급종합병원 간호사의 사전연명의료의향서에 대한 지식, 자신감 및 교육요구도)

  • Jang, Nan-Soon;Park, Hae-Sook;Kim, Mi-Ra;Lee, Joo-Yeon;Cho, Yeo-Won;Kim, Kyoung-Mi;Son, Youn-Jung
    • Journal of Korean Critical Care Nursing
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    • v.11 no.1
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    • pp.35-45
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    • 2018
  • Purpose : Nurses' knowledge regarding advance directives may affect their administration of and confidence towards end of life care. This study aimed to describe the relationships of knowledge, confidence, and learning needs with advance directives among hospital nurses. Method : This cross-sectional study was performed at a tertiary university hospital in Seoul between September 25 and October 14, 2017. Convenience sampling was used to recruit nurses who provided bedside care and had at least 1 year of clinical experience. We used a validated self-report questionnaire. Results : The mean score of knowledge, confidence and learning needs were $5.00{\pm}1.73$, $29.81{\pm}7.52$, and $64.54{\pm}8.48$ respectively. Hospital nurses' knowledge, confidence and learning needs were significantly different according to age, job position, educational level and perceived advance directives. Knowledge regarding advance directives was significantly associated with confidence (r = .27, p < .001) and learning needs (r = .16, p = .005). Conclusion : Knowledge regarding advance directives was relatively low compared to the findings of previous studies. Therefore, nurses should be knowledgeable and encouraged to initiate advance directives. It is necessary to develop a standardized educational program regarding advance directives based on Korean cultures.

Effect of Diffuser Locations on the Room Acoustical Parameters in 1:25 Scale Model Hall (1:25 축소모형 홀에서 확산체의 설치부위에 따른 실내 음향지표의 변화)

  • Kim, Yong-Hee;Seo, Choon-Ki;Lee, Hye-Mi;Jeon, Jin-Yong
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.3
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    • pp.115-128
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    • 2012
  • This paper investigates the effects of diffuser on the acoustical parameters in music hall with consideration of the result of scattering coefficient measurement. A scale model hall of 600 seats with orchestra shell was used for experiments. The materials of 1:50 scale model was chosen through absorption coefficient measurement based on ISO 354. The model was matched to the computer simulation model in terms of reverberation time. In order to evaluate the effect of diffuser location, the measurements were accomplished with and without diffusers according to 7 configurations by diffuser-installed region; sidewall, balcony front, ceiling and so on. The following acoustical parameters were extracted from each measurement case; Reverberation time (RT), Early decay time (EDT), Clarity (C80), Center time (Ts), Sound strength (G) and Temporal diffusion (TD) from the auto-correlation function (ACF) of impulse responses. As a result, the absorption power and diffusion power were increased with number of diffusers. Accordingly RT, EDT and G were decreased by diffuser and the redirection of reflections was occurred briskly. Averaged TD was 6.05 to 6.30 by measurement cases. RT was found to be the most related factor to diffusion power (R = 0.94). The correlation between TD and EDT was high (R = 0.73). In addition, the effects of diffuser-installed location were discussed in terms of acoustical parameter variation.

Rapid Detection of Salmonella spp. in Fresh-Cut Cabbage by Real-Time PCR (Real-Time PCR을 이용한 신선편이 양배추에서 Salmonella spp.의 신속검출)

  • Bang, Mi-Kyung;Park, Seung-Ju;Kim, Yun-Ji;Kim, Ji-Gang;Oh, Se-Wook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.10
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    • pp.1522-1527
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    • 2010
  • This study was conducted to find out the minimal time needed for detection of Salmonella spp. which exist at very low concentration in foods by using real-time PCR. The sal-F and sal-R sequences were used as primers and sal-P was used as a probe. The detection limit of Salmonella spp. was $3.77{\times}10^2\;cfu/mL$ in buffered peptone water (BPW). Microbial growth was monitored after artificially inoculated Salmonella spp. into BPW. The obtained growth curve was well fitted with the equation, y=$0.0127x^2$+0.5927x-0.4317 ($R^2$=0.99), if assuming that 1 cell exists in 25 g sample (0.04 cfu/mL). The microbial concentration will be reduced to 10 fold by adding BPW during sample treatment, so actual initial concentration at the starting point of enrichment is 0.004 cfu/mL. At this condition, real-time PCR detection would be possible only when microbial concentration increase occurs to exceed the detection limit (377 cfu/mL). The time needed for microbial increase was calculated from the growth curve equation as 7 hours and 20 minutes. Therefore the total time required for detection was less than 10 hours including the PCR operating time.

Preparation and Evaluation of Poly(methylmethacrylate-co-trimethoxysilylpropylmethacrylate) Spheres Containing Whitening and Anti-wrinkle Agents (미백 및 주름개선 성분을 함유하는 Poly(methylmethacrylate-co-trimethoxysilylpropylmethacrylate) 구의 제조와 평가)

  • Jung, Taek-Kyu;Lim, Mi-Sun;Kim, Young-Back;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.32 no.1 s.55
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    • pp.35-44
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    • 2006
  • Recently, encapsulation studies have been tarried out to protect active agents using shell materials such as polymers, lipids, inorganic materials and the other protective materials. We have prepared copolymers of methylmethacrylate (MMA) and trimethoxysilylpropylmethacrylate (TMPMA), and the copolymers as shell materials were used for encapsulating active agents. Poly(MMA-co-TMPMA) spheres were very efficient for encapsulating active agents such as vitamin derivatives (such as retinol, retinyl palmitate, tocopheryl acetate and ascorbyl tetraisopalmitate) and oil soluble licorice extract etc. Mean diameters of poly(MMA-co-TMPMA) core-shell spheres containing active agents varied between about 0.1 to $10{\mu}m$ according to the experimental conditions. The loading amount of encapsulating active agents was 15 to 25% (w/w) and the loading yield was above 90%. The stability of active agents in poly(MMA-co-TMPMA) core-shell spheres prepared with an UV absorbing precursor increased by 25% compared with that of active agents in spheres prepared without an UV absorbing precursor.

Effects of Character, Critical Thinking Disposition and Professional Self-Concept on College Adjustment in Nursing Students (간호대학생의 인성, 비판적 사고성향, 전문직 자아개념이 대학생활적응에 미치는 영향)

  • Lim, Mi-Hye
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.560-568
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    • 2016
  • The purpose of this study was to investigate the level of character, critical thinking disposition, professional self-concept, and adjustment to college life, as well as to identify the factors influencing the adjustment to college life in nursing students. The study was conducted on 166 nursing students in C and J Provinces, between May 25th and June 10th 2016. The data was analyzed using t-test, one-way ANOVA, Pearson's correlation, and multiple regression. The mean score of the adjustment to college life was 3.27 (${\pm}0.56$), character was 4.34 (${\pm}0.59$), critical thinking disposition was 3.48 (${\pm}0.40$), and professional self-concept was 2.76(${\pm}0.32$). There were positive correlations between adjustment to college life and character (r=.512, p<0.001), as well as between critical thinking disposition (r=.508, p<0.001) and professional self-concept (r=.614, p<0.001). The significant factors influencing the adjustment to college life were character, critical thinking disposition, and professional self-concept, which explained 43% of the variance in the adjustment to college life. Based on these findings, programs that develop character, critical thinking disposition, and professional self-concept are necessary for college nursing students in order to promote college adjustment.

The Stabilization of 20.0% Ascorbic Acid in Aqueous Cosmetic Formulation (아스코빅애씨드 고함량 안정화 수계 조성물 제조 방법)

  • Park, Jeong Mi;Eun, So Hee;Ko, Eun Ah;Han, Sang Keun;Kang, Hak Hee;Hyun, Seung Min
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.2
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    • pp.125-131
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    • 2018
  • Ascorbic acid (Vitamin C) has been widely used in skin care formulations. Due to its remarkable effects on anti-oxidation, collagen biosynthesis and whitening, ascorbic acid is considered as an effectible anti-aging active ingredient. But, the instability problems of ascorbic acid in cosmetic formulation such as oxidation, browning and changes in smell is the difficult issue to be overcome for the application of high concentration of ascorbic acid. We tried to stabilize the ascorbic acid in non-aqueous liquid formulation that contains polyol solvent at first. The non-aqueous system was effectible to reduce oxidation. But, ascorbic acid was crystallized in the non-aqueous formulation at the low temperature below $5^{\circ}C$. We tried to develop way to stabilize the ascorbic acid in aqueous solutions to solve the crystallizing problem. In this study, we search the optimal ratio of antioxidant combination, such as zinc sulfate, glutathione and curcuma longa (turmeric) root extract. Formulations were stored at - $16^{\circ}C$, $5^{\circ}C$, $25^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$ and cycle($5-40^{\circ}C$) (in incubator) for a period of eight weeks to investigate their stability. In the stability analysis, the test parameters consisted of color, scent, phase separation and sedimentation. Ascorbic acid stability was checked by HPLC analysis.

Factors Influencing the Retention Intention of Nurses in General Hospital Nurses (종합병원 간호사의 재직의도에 영향을 미치는 요인)

  • Kim, Yu-Mi;Seo, Minjeong
    • Journal of Digital Convergence
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    • v.18 no.12
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    • pp.377-387
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    • 2020
  • The purpose of this study is a descriptive research study to identify the emotional labor, ethical climate, job satisfaction, and retention intention of nurses in general hospitals, and to identify the relationship between variables and factors influencing the retention intention. The study subjects were 198 nurses working in four general hospitals, and data were collected from September 18 to September 30, 2019 using a structured questionnaire. The collected data were analyzed by t-test, ANOVA, Pearson's correlation coefficients, and multiple regression using SPSS WIN 25.0. As a result of the study, there was a positive correlation between ethical climate(r=.345, p<.001) and job satisfaction(r=.388, p<.001), but emotional labor (r=-.153, p=.031) showed negative correlation. Factors influencing the retention intention of general hospital nurses are job satisfaction(β=.27, p=.001), age(β=.27, p<001), ethical climate(β=.16, p=044), and the explanatory power of the variable was 24.2%. In order to increase the retention intention of general hospital nurses, it is necessary to increase job satisfaction and create a sound ethical climate, and to develop an intervention program that can increase the retention intention by age.