• Title/Summary/Keyword: miR-20a

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Influences of Compassion Satisfaction, Compassion Fatigue, and Burnout on Positive Psychological Capital of Clinical Nurses (임상간호사의 공감만족, 공감피로, 소진이 긍정심리자본에 미치는 영향)

  • Jeong, Eun;Jung, Mi-Ra
    • The Journal of the Korea Contents Association
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    • v.18 no.3
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    • pp.246-255
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    • 2018
  • The purpose of this study was to investigate factors affecting positive psychological capital in clinical nurses. The data were collected from 193 nurses working in the three general hospital located Y city using self-reported questionnaires and November 20 2017 to November 30 2017. The data were analyzed by descriptive statistics, T-test, one-way ANOVA, Pearson's correlation coefficient, and stepwise multiple regression with SPSS 20.0 program. Positive psychological capital had a significant positive effect on compassion satisfaction, while Positive psychological capital had a negative effect on burnout. The result of the multiple regression indicates the compassion satisfaction, burnout and job position predict 55.5% (F=30.88, p<.001) of positive psychological capital. Therefore, strategies to decrease burnout and to enhance compassion satisfaction for clinical nurses.

Bacterial Community Analysis and Antibacterial Activity Isolated from Umbraulva japonica (초록갈파래(Umbraulva japonica)에서 분리한 세균의 군집 구조 분석 및 항균 활성)

  • Kim, Ji-Hyun;Park, So-Hyun;Moon, Kyung-Mi;Kim, Dong-Hwi;Heo, Moon-Soo
    • Microbiology and Biotechnology Letters
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    • v.46 no.2
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    • pp.127-134
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    • 2018
  • In this study, 79 bacterial isolates were collected from the surface of marine algae Umbraulva japonica. As a result of analysis of 16s rRNA gene sequence, the 79 isolated bacteria were divided into 4 major groups: [Proteobacteria (74.69%), Actinobacteria (2.53%), Fimicutes (2.53%), and Bacteroidetes (20.25%)] - 7 classes (Actinobacteria, Flavobacteria, Sphingobacteria, Baciili, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria), 12 orders, 17 families and 31 genera. The newly isolated 3 strains could be novel species because of less than 97% similarity in 16s rRNA sequence. Therefore, it is considered that additional experiments should be conducted together with the standard strain. Analysis of 79 bacterial antibacterial activity against human and fish pathogens, such as Edwardsiella tarda, Vibrio harveyi, Streptococcus iniae, Steptococcus parauberis, Escherichia coli, Steptococcus mutans, Listeria monocytogenes and Vibrio vulnificus, was performed by using the supernatant liquid and pellet. As a result, pellet of UJT9, UJT20 and UJR17 showed antibacterial activity against V. vulnificus, UJR17 also showed antibacterial activity against S. parauberis. UJT7 and UJT20, UJR17 have been identified as Bacillus sp. and Pseudomonas sp. and it may be safely assented that it's beneficial to carry out additional experiments for various applications.

A New Analytical Method for Erythromycin in Fish by Liquid Chromatography/Tandem Mass Spectrometry

  • Park, Mi-Jung;Park, Mi-Seon;Lee, Tae-Seek;Shin, Il-Shik
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.508-513
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    • 2008
  • Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

Characteristic of Oviposition and Effect of Density Suppression by Yellow-colored Sticky Trap on Ricania shantungensis (Hemiptera: Ricaniidae) in Blueberry (블루베리에서 갈색날개매미충의 산란특성 및 황색끈끈이트랩의 산란 억제효과)

  • Kim, Dong Hwan;Kim, Hyeong Hwan;Yang, Chang Yeol;Kang, Taek Jun;Yoon, Jung Beom;Seo, Mi Hye
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.281-285
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    • 2016
  • This study was conducted to investigate the characteristics of oviposition and the effect of density suppression by yellow-colored sticky trap on Ricania shantungensis in blueberry. The occurrence of an egg mass of R. shantungensis in the upper, middle and lower region were 56.6~60.2%, 23.8~28.1% and 11.7~19.7%, respectively. The number of egg masses in the branch was investigated. Percentage of the branch with one egg mass was greatest (50.9%) than with two (20.5%), three (14.6%) and over four (14.0%). The effect of yellow-colored sticky trap to reduce the number of R. shantungensis egg masses in blueberry was also investigated. In a month after yellow sticky trap installation, 17.1 adults of R. shantungensis were attracted per trap. Moreover, the number of egg masses on a tree in yellow-colored sticky trap plot was much lower (0.4) than control (1.3). Consequently, this result shows that use of yellow-colored sticky trap may contribute to decrease ovipisotion rate of R. shantungensis in blueberry.

A Novel Multi-focus Image Fusion Scheme using Nested Genetic Algorithms with "Gifted Genes" (재능 유전인자를 갖는 네스티드 유전자 알고리듬을 이용한 새로운 다중 초점 이미지 융합 기법)

  • Park, Dae-Chul;Atole, Ronnel R.
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.9 no.1
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    • pp.75-87
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    • 2009
  • We propose in this paper a novel approach to image fusion in which the fusion rule is guided by optimizing an image clarity function. A Genetic Algorithm is used to stochastically select, comparative to the clarity function, the optimum block from among the source images. A novel nested Genetic Algorithm with gifted individuals found through bombardment of genes by the mutation operator is designed and implemented. Convergence of the algorithm is analytically and empirically examined and statistically compared (MANOVA) with the canonical GA using 3 test functions commonly used in the GA literature. The resulting GA is invariant to parameters and population size, and a minimal size of 20 individuals is found to be sufficient in the tests. In the fusion application, each individual in the population is a finite sequence of discrete values that represent input blocks. Performance of the proposed technique applied to image fusion experiments, is characterized in terms of Mutual Information (MI) as the output quality measure. The method is tested with C=2 input images. The results of the proposed scheme indicate a practical and attractive alternative to current multi-focus image fusion techniques.

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Effect of Bacillus mesonae H20-5 Treatment on Rhizospheric Bacterial Community of Tomato Plants under Salinity Stress

  • Lee, Shin Ae;Kim, Hyeon Su;Sang, Mee Kyung;Song, Jaekyeong;Weon, Hang-Yeon
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.662-672
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    • 2021
  • Plant growth-promoting bacteria improve plant growth under abiotic stress conditions. However, their effects on microbial succession in the rhizosphere are poorly understood. In this study, the inoculants of Bacillus mesonae strain H20-5 were administered to tomato plants grown in soils with different salinity levels (EC of 2, 4, and 6 dS/m). The bacterial communities in the bulk and rhizosphere soils were examined 14 days after H20-5 treatment using Illumina MiSeq sequencing of the bacterial 16S rRNA gene. Although the abundance of H20-5 rapidly decreased in the bulk and rhizosphere soils, a shift in the bacterial community was observed following H20-5 treatment. The variation in bacterial communities due to H20-5 treatment was higher in the rhizosphere than in the bulk soils. Additionally, the bacterial species richness and diversity were greater in the H20-5 treated rhizosphere than in the control. The composition and structure of the bacterial communities varied with soil salinity levels, and those in the H20-5 treated rhizosphere soil were clustered. The members of Actinobacteria genera, including Kineosporia, Virgisporangium, Actinoplanes, Gaiella, Blastococcus, and Solirubrobacter, were enriched in the H20-5 treated rhizosphere soils. The microbial co-occurrence network of the bacterial community in the H20-5 treated rhizosphere soils had more modules and keystone taxa compared to the control. These findings revealed that the strain H20-5 induced systemic tolerance in tomato plants and influenced the diversity, composition, structure, and network of bacterial communities. The bacterial community in the H20-5 treated rhizosphere soils also appeared to be relatively stable to soil salinity changes.

The Crystal Structure of Bis(N-Methylphenazinium) Bis(Oxalato)Palladate(Ⅱ) (Bis(N-Methylphenazinium) Bis(Oxalato)Palladate(Ⅱ)의 결정구조)

  • Kim, Se Hwan;NamGung, Hae;Lee, Hyeon Mi
    • Journal of the Korean Chemical Society
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    • v.38 no.11
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    • pp.827-832
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    • 1994
  • The crystal structure of bis(N-methylphenazinium) bis(oxalato)palladate(II) has been determined by X-ray crystallography. Crystal data: ((C_{13}H_{11}N_2)_2[Pd(C_2O_4)_2]) $M_w$ = 672.93, Triclinic, Space Group P1 (No = 2), a = 7.616(8), b = 9.842(3), c = $20.335(7)\AA$, $\alpha$ = 103.53(3), $\beta$ = 90.00(5), $\gamma$ = $112.38(5)^{\circ}$, Z = 2, $V = 1363(2){\AA}^3\;D_c = 1.639\;gcm^{-3},\;{\mu} = 7.3\;cm^{-1},\;F(000) = 680.0$. The intensity data were collected with $Mo-K\alpha$ radiation (${\lambda}$= 0.7107\;\AA)$ on an automatic four-circle diffractometer with a graphite monochromater. The structure was solved by Patterson method and refined by full matrix least-square methods using Killean & Lawrence weights. The final R and S values were $R = 0.069,\;R_w = 0.050,\;R_{all} = 0.069$ and S = 5.45 for 3120 observed reflections. Both cation and anion complexes are essentially planar and have dihedral angles of 6.3(6) and $57.06(6)^{\circ}$ between their planes. The planar complex anions are sandwiched between slightly bent cations. The interplanar separations of two triads are 3.328 and 3.463 $\AA$, respectively. The triads are stacked along b-axis, but their orientations are different based on dihedral angle $59.08(9)^{\circ}$ of two complex anions.

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Natural Antioxidants to Improve Stability of Refined Anchovy Oil against Oxidation

  • Park, D.C.;Jr, Ho-Seok;Lee, Heon;Kim, Jeon-Ju;Jung, Yun-Mi;Gyoung, Young-Soo;Kang, Suk-Nam;Kim, Seon-Bong
    • Food Science and Biotechnology
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    • v.15 no.2
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    • pp.202-206
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    • 2006
  • The oxidation stability of fish oil containing omega-3 polyunsaturated fatty acids (PUFAs), which is very susceptible to oxidative deterioration, needs to be improved before it can be successfully applied to functional foods. The antioxidant activities of 17 species of materials in anchovy oil (AO) were compared and a potent antioxidant was determined to improve the shelf-life of refined AO. Antioxidant activities of the 0.05% (w/w) materials in AO were compared against control during storage at $30^{\circ}C$ for 10 days. While no antioxidant effect was shown in alpha tocopherol against control, 3 species of grapefruit seed extracts (GSEs), astaxanthin (AX), soybean lecithin, and green tea extract showed good antioxidant activities. Especially, GSE B, GSE C, and AX showed significantly high peroxide inhibitory activities (PIAs) of $16.2{\pm}2.1$, $20.{\pm}3.5$, and $17.7{\pm}3.5%$, respectively, after the 4th day (p<0.01). Radical scavenging activities (RSAs) of GSE B, GSE C, and AX were $85.1{\pm}0.8$, $95.3{\pm}0.3$, and $85.9{\pm}0.8%$, respectively. Correlation between PIAs and RSAs was high ($R^2=0.926$) in GSE B, GSE C, and AX. Therefore, we concluded that one of the main antioxidative mechanisms of GSEs and AX must operate through an RSA pathway. The $RC_{50}$ (concentration required for 50% reduction of 1,1-diphenyl-2-picryl-hydrazyl, DPPH) of GSE C was $258\;{\mu}g/mL$.

A Study on Indoor Subjective Symptoms of Workers at Dental Hospitals (치과병원 종사자들의 실내자각증상에 관한 요인분석)

  • Jeong, Mi Ae
    • Proceedings of the Korea Contents Association Conference
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    • 2012.05a
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    • pp.345-346
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    • 2012
  • 본 연구는 치과진료실의 실내 공기질에 영향을 미치는 요인과 종사자의 실내자각증상과 관련성을 조사하여 치과위생사 개인의 발전과 건강 증진에 필요한 기초자료를 마련하고자 본 연구를 시행하였다. 전국(서울, 경기, 대구, 포항)의 종합병원, 치과병원, 치과의원의 치과위생사 210명을 대상으로 2011년 10월 1일부터 20일까지 자기기입식 설문지 조사를 시행한 후 회수하여 응답이 불분명한 5명을 제외한 205명에 대해서 분석 하였다. 수집된 자료는 SPSS WIN 15.0 프로그램을 이용하여 분석하였다. 그 결과 근무 중 휴식시간 유무별로는 근무 중 휴식시간이 없는 치과위생사가 67.3%로 높은 분포를 보였고, 건물 밖 외출 여부별로는 때때로가 54.6%로 절반 이상을 차지하였으며, 건물 밖 외출횟수별로는 1회 86.1%로 대부분을 차지하였으며, 근무기관의 계절별 자연환기 정도별로는 봄, 여름, 가을, 겨울 모두 1일 2회 이내가 절반 이상을 차지하였으며, 다음으로 1일 4회 이내 19.0%, 기타 9.8%, 1일 10회 이내와 하루 종일 2.4% 순이었다. 실내자각증상 호소율에 대해 살펴본 결과는 4점 만점 중 전체 평균이 2.45로 나타났다. 먼저 영역별로는 평균이 '목증상'과 '눈증상'이 각각 2.54로 가장 높았으며, 문항 중에는 평균이 '눈이 잘 피로해진다'가 3.00으로 가장 높게 나타났다. 근무환경 특성에 따른 실내자각증상은 야간진료 여부별로는 야간진료를 하는 치과위생사가 그렇지 않은 치과위생사보다 실내자각증상이 낮았으며, 야간진료 여부에 따라 유의한 차이를 보였다(t=2.07, p<.05). 실내자각증상과 직무스트레스의 관계는 물리환경(r=.259, p<.001)과 직무요구(r=.311, p<.001), 관계갈등(r=.172, p<.05), 조직체계(r=.158, p<.05), 보상 부적절(r=.197, p<.01), 그리고 직무스트레스(r=.275, p<.001)와 통계적으로 유의한 상관관계를 보였다.

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Experimental Infection for the Neutralization of White Spot Syndrome Virus (WSSV) in Wild Captured Sand Shrimp, Crangon affinis (자연산 자주새우(Crangon affinis)에서 흰반점바이러스(WSSV)의 neutralization을 위한 인위감염(experimental infection))

  • Gong, Soo-Jung;Kim, Yeong-Jin;Choi, Mi-Ran;Kim, Sung-Koo
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1294-1298
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    • 2010
  • White spot syndrome virus (WSSV) is one of the most virulent viral agents threatening the penaeid shrimp culture industry. This study was carried out to evaluate the susceptibility of the sand shrimp, Crangon affinis, to WSSV as an alternative experimental model. WSSV caused 100% mortality in C. affinis within 7 days after experimental infection by immersion. Based on challenge studies, it was confirmed that C. affinis could be a potential host in WSSV transmission. Also, the neutralization of WSSV was carried out using an antiserum raised against recombinant envelop protein rVP466 to evaluate the WSSV infection mechanism. A constant amount of WSSV (at $1{\times}10^4$ diluted stocks) was incubated with various amounts of antiserum and then mixed to 20 l reservoir for the immersion challenge of C. affinis for neutralization. At 5 days post challenge, the shrimp in the positive control immersed in the immersion reservoir containing WSSV stock showed 100% mortality. The shrimps challenged with the 3 different mixtures of WSSV and rVP466 antiserum (1:0.1, 1:0.5 and 1:1) showed 100%, 68.8% and 68.8% mortality at 14 days post challenge, respectively. These results indicated that the antiserum raised against rVP466 could block WSSV infection in C. affinis. Therefore, this study confirmed that C. affinis can be naturally infected by WSSV as another potential host and that C. affinis can be used as an alternative experimental animal instead of penaeid shrimps.