Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.9.1294

Experimental Infection for the Neutralization of White Spot Syndrome Virus (WSSV) in Wild Captured Sand Shrimp, Crangon affinis  

Gong, Soo-Jung (Research Center, Binex)
Kim, Yeong-Jin (Department of Biotechnology, Pukyong National University)
Choi, Mi-Ran (Department of Biotechnology, Pukyong National University)
Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
Publication Information
Journal of Life Science / v.20, no.9, 2010 , pp. 1294-1298 More about this Journal
Abstract
White spot syndrome virus (WSSV) is one of the most virulent viral agents threatening the penaeid shrimp culture industry. This study was carried out to evaluate the susceptibility of the sand shrimp, Crangon affinis, to WSSV as an alternative experimental model. WSSV caused 100% mortality in C. affinis within 7 days after experimental infection by immersion. Based on challenge studies, it was confirmed that C. affinis could be a potential host in WSSV transmission. Also, the neutralization of WSSV was carried out using an antiserum raised against recombinant envelop protein rVP466 to evaluate the WSSV infection mechanism. A constant amount of WSSV (at $1{\times}10^4$ diluted stocks) was incubated with various amounts of antiserum and then mixed to 20 l reservoir for the immersion challenge of C. affinis for neutralization. At 5 days post challenge, the shrimp in the positive control immersed in the immersion reservoir containing WSSV stock showed 100% mortality. The shrimps challenged with the 3 different mixtures of WSSV and rVP466 antiserum (1:0.1, 1:0.5 and 1:1) showed 100%, 68.8% and 68.8% mortality at 14 days post challenge, respectively. These results indicated that the antiserum raised against rVP466 could block WSSV infection in C. affinis. Therefore, this study confirmed that C. affinis can be naturally infected by WSSV as another potential host and that C. affinis can be used as an alternative experimental animal instead of penaeid shrimps.
Keywords
White spot syndrome virus (WSSV); Crangon affinis; VP466; antiserum; neutralization;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Wang, Y. C., C. F. Lo, P. S. Chang, and G. H. Kou. 1998. Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture 164, 221-231.   DOI
2 Witteveldt, J., C. C. Cifuentes, J. M. Vlak, and Van M. C. W. Hulten. 2004. Protection of Penaeus monodon against white spot syndrome virus by oral vaccination. J. Virol. 78, 2057-2061.   DOI
3 Wu, J. L., T. Nishioka, K. Mori, T. Nishizawa, and K. A. Muroga. 2002. A time-course study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol. 13, 391-403.   DOI
4 Wu, W., L. Wang, and X. Zhang. 2005. Identification of white spot syndrome virus (WSSV) envelop proteins involved in shrimp infection. Virology 332, 578-583.   DOI
5 Harlow, E. and D. Lane. 1988. Antibodies: a laboratory manual. NY, USA: Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
6 Herold, B. C., D. WuDunn, N. Soltys, and P. G. Spear. 1991. Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity. J. Virol. 65, 1090-1098.
7 Huang, C. H., X. B. Zhang, Q. S. Lin, X. Xu, Z. H. Hu, and C. L. Hew. 2002. Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelop protein VP466. Mol. Cell Proteomics 1, 223-231.   DOI
8 Musthaq, S. S., K. Yoganandhan, R. Sudhakaran, S. R. Kumar, and A. S. Hameed. 2006. Neutralization of white spot syndrome virus of shrimp by antiserum raised against recombinant VP28. Aquaculture 253, 98-104.   DOI
9 Tsai, J. M., H. C. Wang, J. H. Leu, H. H. Hsiao, A. H. J. Wang, G. H. Kou, and C. F. Lo. 2004. Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J. Virol. 78, 11360-11370.   DOI
10 Poo, H. R., J. J. Song, S. P. Hong, Y. H. Choi, S. W. Yun, J. H. Kim, S. C. Lee, S. G. Lee, and M. H. Sung. 2002. Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-$\alpha$. Biotechnol. Lett. 24, 1185-1189.   DOI
11 Van Hulten, M. C. W., J. Witteveldt, M. Snippe, and J. M. Vlak. 2001. White spot syndrome virus envelop protein VP28 is involved in the systemic infection of shrimp. Virology 285, 228-233.   DOI
12 Volkman, L. E. and P. A. Goldsmith. 1985. Mechanism of neutralization of budded Autographs californica nuclear polyhedrosis virus by a monoclonal antibody: Inhibition of entry by adsorptive endocytosis. Virology 143, 185-195.   DOI
13 Ha, Y. M., Y. I. Kim, K. H. Kim, and S. K. Kim. 2008. Neutralization of white spot syndrome virus (WSSV) for Penaeus chinensis by antiserum raised against recombinant VP19. J. Environ. Biol. 29, 513-517.
14 Burton, D. R. 2002. Antibodies, viruses and vaccines. Natl. Rev. Immunol. 2, 706-713.   DOI
15 Chou, H. Y., C. Y. Huang, C. H. Wang, H. C. Chiang, and C. F. Lo. 1995. Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis. Aquat. Org. 23, 165-173.   DOI