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http://dx.doi.org/10.4014/mbl.1803.03016

Bacterial Community Analysis and Antibacterial Activity Isolated from Umbraulva japonica  

Kim, Ji-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Life Science, Jeju National University)
Park, So-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Life Science, Jeju National University)
Moon, Kyung-Mi (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Life Science, Jeju National University)
Kim, Dong-Hwi (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Life Science, Jeju National University)
Heo, Moon-Soo (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Life Science, Jeju National University)
Publication Information
Microbiology and Biotechnology Letters / v.46, no.2, 2018 , pp. 127-134 More about this Journal
Abstract
In this study, 79 bacterial isolates were collected from the surface of marine algae Umbraulva japonica. As a result of analysis of 16s rRNA gene sequence, the 79 isolated bacteria were divided into 4 major groups: [Proteobacteria (74.69%), Actinobacteria (2.53%), Fimicutes (2.53%), and Bacteroidetes (20.25%)] - 7 classes (Actinobacteria, Flavobacteria, Sphingobacteria, Baciili, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria), 12 orders, 17 families and 31 genera. The newly isolated 3 strains could be novel species because of less than 97% similarity in 16s rRNA sequence. Therefore, it is considered that additional experiments should be conducted together with the standard strain. Analysis of 79 bacterial antibacterial activity against human and fish pathogens, such as Edwardsiella tarda, Vibrio harveyi, Streptococcus iniae, Steptococcus parauberis, Escherichia coli, Steptococcus mutans, Listeria monocytogenes and Vibrio vulnificus, was performed by using the supernatant liquid and pellet. As a result, pellet of UJT9, UJT20 and UJR17 showed antibacterial activity against V. vulnificus, UJR17 also showed antibacterial activity against S. parauberis. UJT7 and UJT20, UJR17 have been identified as Bacillus sp. and Pseudomonas sp. and it may be safely assented that it's beneficial to carry out additional experiments for various applications.
Keywords
Antibacterial activity; bacterial community; marine algae; marine bacteria; Umbraulva japonica;
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