• Title/Summary/Keyword: miR-152

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Predictors of Vascular Complications among Patients with Type 2 Diabetes (제2형 당뇨 환자의 혈관합병증 위험 예측인자)

  • Ha, Jung-Mi;Lee, Hae-Jung;Kim, Dong-Hee;Kim, Yong-Suk;Lee, Wha-Za
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.16 no.2
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    • pp.144-152
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    • 2009
  • Purpose: The purpose of this study was to predict the risk factors for vascular complications among patients with type 2 diabetes. Method: The data were collected from August to September, 2007 using clinical examination and questionnaires. Patients (N=101) were recruited from the endocrinology department of P University hospital in D city. Descriptive statistics, Pearson correlation coefficients and multiple linear regression were used to analyze the data. Results: The cardiovascular risk of patients with diabetes was significantly related to self care behavior, family history, and smoking status. The risk of peripheral vascular complications was not related to predictors included in the study. With multivariate analyses, significant predictors of cardiovascular risk for these patients were self care behavior, family history, and smoking status ($R^2=.40$, p<.0001). Conclusion: The findings of this study indicate that smoking cessation and improving self-care behavior are essential to reduce the risk of cardiovascular complications among patients with diabetes. To enhance self-care practices for the patients with diabetes, nursing interventions, such as telephone counseling, problem focused nursing counseling, and peer group activities should be considered.

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Nursing Students' Knowledge, Attitudes, Perceived Behavior Control and Intention to Report Cases of Child Abuse (간호대학생의 아동학대 지식, 태도, 지각된 행위통제력과 아동학대 사례별 신고의도)

  • Cho, Kyung-Mi;Kim, Eun-Joo
    • Child Health Nursing Research
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    • v.22 no.2
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    • pp.145-152
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    • 2016
  • Purpose: This study was designed to explore nursing students' knowledge, attitudes and intention to report cases of child abuse. Methods: A descriptive research design was used with a convenience sample of 202 nursing students. After explaining the purpose of the study, participants who signed the consent form were asked to complete a self-descriptive questionnaire, which included the Child Abuse Intension Scale (CARIS). Results: The number of correct answers for knowledge of child abuse and the law was 7.0 /13. The mean scores were, for attitude toward childrearing belief and discipline, $17.1{\pm}5.2$, for punishment and culpability of offender or victim, $24.6{\pm}4.1$, for professional responsibility, $30.5{\pm}5.1$, and for perceived behavioral control, $25.3{\pm}5.0$. The intended reporting behavior differed significantly by severity of abuse. Factors influencing the intention to report child abuse were attitude towards punishment of parents, professional responsibility, and perceived behavioral control ($R^2$=.133). Conclusion: On the basis of our finding, developing education programs to help nursing students detect child abuse and improve reporting rates is important. Thus, we suggests that nursing students be provided with educational protocol for detection and reporting of child abuse.

Assessment of Daily steps, Activity coefficient, Body composition, Resting Energy Expenditure and Daily Energy Expenditure in Female University Students (여대생의 1일 보행수, 활동계수, 신체조성, 휴식대사량 및 에너지 소비량의 평가)

  • Choe, Hyeon-Jeong;Song, Ju-Mi;Kim, Eun-Gyeong
    • Journal of the Korean Dietetic Association
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    • v.11 no.2
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    • pp.159-169
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    • 2005
  • The purpose of this study was to assess the energy expenditure and investigate the relationship between related variables in 70 female university students. Resting energy expenditure estimated by Harris-Benedict formula, WHO/NAO/FAO formula and various formulas based on body weight and body surface area were 1366.9$\pm$74.4kcal/day, 1287.8$\pm$106.6kcal/day, 1171.4$\pm$155.8kcal/day and 1342.0$\pm$97.4kcal/day. Measured resting energy expenditure by indirect calorimetry(Model : Metavine and TrueOne2400) were 1582.0$\pm$150.1kcal/day and 1268.2$\pm$152.9kcal/day, respectively. Average step number per day was 11981.2$\pm$3014.4 steps and average step number per hour was 746.1$\pm$198.0 steps/hr. Daily energy expenditure by using Harris-Benedict formula, body weight formula, body surface area formula, WHO/NAO.FAO formula and 15-min check list formula were 2374.7$\pm$249.6kcal, 2033.5$\pm$313.2kcal, 2331.2$\pm$266.0kcal, 2240.8$\pm$185.5kcal and 2195.5$\pm$398.3kcal. Meanwhile energy intake of subjects was 1714.9$\pm$551.2 kcal. Daily energy expenditure has positive correlation(r=0.262) with daily step number. And there was significant positive correlations(r=0.35-0.68) between various daily energy expenditures and muscle mass. These results suggested that increase of daily step number by using pedometer is good method to increase daily energy expenditure. In particular, increase in step number can reinforce the amounts of muscle.

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A study on factors associated with job stress and drinking levels in emergency room nurses (응급실 간호사의 직무스트레스와 음주 관계 연구)

  • Kwak, Eun-mi;Lee, Joo Young;Yun, Heejang
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.2
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    • pp.145-152
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    • 2020
  • The objective of this study was to examine the degree of job stress and drinking levels among emergency room nurses and to investigate the association between these variables. Data were collected using self-reported questionnaires completed by 106 nurses working in emergency room of hospitals from 3 different cities. The collected data were analyzed using SPSS 21.0 program. The results showed that the mean job stress score was 45.93±6.81 points, while 18.9% of the subjects were in the disordered alcohol use group. Differences in drinking levels were found according to the work experience of the nurses (χ2=8.249, p=.041), while significant differences in job stress scores were found according to marital status (t=-2.72, p=.008) and desire to continue working (t=-2.272, p=.025). There was a significant correlation between job stress and drinking level (r=-.147, p= .000). Measures to lower job stress among emergency room nurses are need based on the findings in the study and it is expected that decreasing the drinking level of nurse would contribute to this.

A Study on the Turnover Intention and Job Satisfaction of Dental Hygienists (치과위생사의 이직의도와 직무만족도에 관한 연구)

  • Yoon, Mi-Sook;Lee, Kyung-Hee;Choi, Mi-Sook
    • Journal of dental hygiene science
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    • v.6 no.3
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    • pp.147-152
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    • 2006
  • The purpose of this study was to help decrease turnover and to make full use of dental hygienists and to help improve management by managers. After relevant literature and data were reviewed, a survey was conducted on dental hygienists, who worked at dental institutes in different areas, for about four months from September through December, 2004, to find out their turnover intention and job satisfaction level. When the collected data were analyzed, the following findings were given: 1. The collective average of the dental hygienists investigated in job satisfaction was $3.30{\pm}0.70$. By area, they expressed the best satisfaction at a clear role factors with a mean of $3.53{\pm}0.73$, and they were pleased with wages factors and promotion factors the least with an average of $3.14{\pm}0.70$ and $3.18{\pm}0.80$. 2. The collective average of the dental hygienists investigated in turnover intention was $3.23{\pm}0.77$. As for connections between general characteristics and turnover intention, By age, those who aged in 21 and 25 were most intended(p < 0.001). The unmarried dental hygienists were more intended than the married ones(p < 0.001). By type of hospital, those who worked at dental clinics were most intended(p< 0.01). By career, those who had worked at one to three years were intended the most(p < 0.001), and as to the length of service at current work places, those who had worked at their current work places for one to three years were intended the most(p < 0.001). By monthly mean pay, those whose pay ranged from 1.3 to 1.6 million won found their turnover most intended(p< 0.01). 3. Regarding correlation among turnover intention and job satisfaction were both inversed correlationship, and wages factor(r = -0.249, p < 0.01) and interrelationship factors(r = -0.218, p < 0.01) had a strong correlation to turnover intention, and clear role factors had a strong correlation to job contents factors by job satisfaction(r = 0.612, p < 0.01), and job satisfaction factors had a strong correlation to job contents factors(r = 0.759, p < 0.01).

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Detection of Mutations to Zidovudine in the pol Gene of Human Immunodeficiency Virus-1 by Direct Sequencing (인면역결핍 바이러스 pol 유전자 염기서열 결정에 의한 지도부딘 (ZDV) 내성 돌연변이의 탐지)

  • Cho, Young-Keol;Lee, Hee-Jung;Sung, Heung-Sup;Kim, Yoo-Kyum;Kim, Young-Bong;Lee, Yong-Jin;Kim, Mi-Jung;Kim, Dae-Ghon;Won, Young-Ho;Cho, Goon-Jae
    • The Journal of Korean Society of Virology
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    • v.29 no.4
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    • pp.271-281
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    • 1999
  • The nested polymerase chain reaction (PCR) assay was used to determine the sequences of reverse transcriptase (RT) codons 41, 67, 70, 210, 215 and 219 of human immunodeficiency virus-1 (HIV-1) pol gene. Template DNA was obtained from uncultured peripheral blood mononuclear cells from 27 Korean HIV-1 infected patients treated with ZDV and Korean red ginseng. The second PCRs were done for 2 separated regions (RT codons $13{\sim}98$ and $152{\sim}259$) with $5\;{\mu}l$ of the first PCR productNucleotide sequences were determined by direct sequencing. In the 27 patients, CD4+ cell count decreased from $230{\pm}117/{\mu}l$ to $152{\pm}162/{\mu}l$ for $46{\pm}26$ months (Mo), and actual duration of ZDV intake was $72{\pm}16$ Mo. In the 16 patients who had been treated with ZDV therapy ${\ge}25$ Mo, the incidences of 70R, 215F/Y, and 41L were 61%, 28% and 22%, respectively and those of 67N, 210W and 219Q were 17%. The incidences of 215F/Y were 6.7% for group ${\le}12$ Mo treatment, 22.7% for group with 13 to 24 Mo, and 27.8% for group ${\ge}25$ Mo. There was no mutation in 9 patients. It might be associated with the interruption of ZDV therapy for more than 6 months in 6 patients. This study shows that the detection of mutation could be useful prognostic marker with other clinical and virological data, and very low mutation rate is dectected compared to overseas reports.

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Cellulose Degradation and Extracellulat Enzymatic Activity of the Mud Flat in Sunchon Bay (순천만 갯벌 토양의 섬유소 분해능 및 체외효소 활성)

  • 백근식;최지혁;성치남
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.130-135
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    • 2000
  • Decomposition rate of organic matiter in the mud flat of Sunchon Bay was estimated. Physicochemical parameters, cellulose degradation rate. distribution of heterotrophic bacteria, and extracellular enzymatic activities were measured from August 1997 to July 1998. Soil temperatures, water contents, concentration of $PO_4$-P and organic matter were -1-~$30^{\circ}C$, 42.1-53.1%, 0.0779-0.1961 mgig and 1.99-7.64%, respectively. Decomposition rate of cellulose film ranged from 7.7 to 100%imonth, high in summer and low in winter. The number of heterotrophic bacteria ranged from $0.87{\times}10^6 to 3.6{\times}10^7 $CUFsIg dq soil. Enzymatic activities of phosphatase, $\alpha$-D-gluEosidase, $\beta$-D-glucosidase and cellobiohydrolase, which were measured as decomposition rate of methylumbelliferyl(MLiF)-substrate, were 152.23-1779.80 nMIhr, 2.67-202.18 nM/hr, 5.03-258.26 M h r and 3.42-63.07 nM/hr, respectively Cellulose degradaaon rate and extracellular extracellular enzymatic activities were conelated with each other, and showed high correlation coefticiency with soil temperature.

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Feasibility of near-infrared spectroscopic observation for traditional fermented soybean production (전통 메주 제조과정에 있어서 근적외 모니터링 가능성 조사)

  • Jeon, Jae Hwan;Lee, Seon Mi;Cho, Rae Kwang
    • Food Science and Preservation
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    • v.24 no.1
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    • pp.145-152
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    • 2017
  • In this study, near infrared (NIR) spectroscopy known as a non-destructive analysis technique was applied to investigate peptide cleavage and consequent release of amino acids in soybean lumps as affected by its moisture content and incubation time during fermentation at 25 for 3 weeks. The NIR spectra of the soybean lump semi-dried and soaked in saline water showed that absorption intensity around 1,400 nm originating from hydrogen bonds of water decreased and absorption band shifted to 1,430 nm as moisture content decreased during incubation at 25 for 3 weeks. In addition, absorption around 2,050 nm which was assigned to amino groups increased as incubation time increased. NIR spectra data from 1,000 to 2,250 nm showed higher accuracy in the discriminant analysis between outside and inside parts of fermented soybean lumps than visible spectra result. NIR spectroscopy for the amino acid and moisture contents in traditional fermented soybean lumps showed relatively good accuracy with the multiple correlation coefficient ($R^2$) of 0.91 and 0.81, respectively, and root mean square error of cross validation (RMSECv) of 0.23 and 0.83%, respectively, in partial least square regression (PLSR). These results indicate that NIR spectral observations could be applicable to control the fermentation process for preparation of soybean products.

Development of Species-Specific PCR to Determine the Animal Raw Material (종 특이 프라이머를 이용한 동물성 식품원료의 진위 판별법 개발)

  • Kim, Kyu-Heon;Lee, Ho-Yeon;Kim, Yong-Sang;Kim, Mi-Ra;Jung, Yoo Kyung;Lee, Jae-Hwang;Chang, Hye-Sook;Park, Yong-Chjun;Kim, Sang Yub;Choi, Jang Duck;Jang, Young-Mi
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.347-355
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    • 2014
  • In this study, the detection method was developed using molecular biological technique to distinguish authenticity of animal raw materials. The genes for distinction of species about animals targeted at Cytochrome c oxidase subunit I (COI), Cytochrome b (Cytb), and 16S ribosomal RNA (16S rRNA) genes in mitochondrial DNA. The species-specific primers were designed by that Polymerase Chain Reaction (PCR) product size was around 200 bp for applying to processed products. The target 24 raw materials were 2 species of domestic animals, 6 species of poultry, 2 species of freshwater fishes, 13 species of marine fishes and 1 species of crustaceans. The results of PCR for Rabbit, Fox, Pheasant, Domestic Pigeon, Rufous Turtle Dove, Quail, Tree Sparrow, Barn Swallow, Catfish, Mandarin Fish, Flying Fish, Mallotus villosus, Pacific Herring, Sand Lance, Japanese Anchovy, Small Yellow Croaker, Halibut, Jacopever, Skate Ray, Ray, File Fish, Sea Bass, Sea Urchin, and Lobster raw materials were confirmed 113 bp ~ 218 bp, respectively. Also, non-specific PCR products were not detected in compare species by species-specific primers. The method using primers developed in this study may be applied to distinguish an authenticity of food materials included animal raw materials for various processed products.

Characteristics of the Strains Selected from Crosses between Introduced Interspecific Hybrids and Cultivars in Hibiscus Species (종간교잡 유래 도입 무궁화와 국내 선발 품종과의 교잡에 의해 육성된 계통들의 특성)

  • Kang, Ho-Chul;Ha, Yoo-Mi;Kim, Dong-Yeob;Han, In Song;Noh, Kwang-Mo
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.55-63
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    • 2011
  • This study was carried out to develop new cultivars of Hibiscus species from crosses between introduced interspecific hybrids and cultivars in Hibiscus species. Fruit setting of interspecific crosses of Hibiscus strains was less than 10% and the number of seeds in the fruit was also in low level. Three individuals of specific flower and leaf characteristics were selected from crosses between introduced interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂) in 2004. A new strain, Hibiscus ${\times}$ 'W-26', was selected from the crossing of interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂), which had white flower and narrow separated petal. Hibiscus ${\times}$ 'WRB-2' was selected from the crossing of interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂), which had white flower and blue eye spot. Hibiscus ${\times}$ 'R-141' was selected from crosses between introduced interspecific hybrid, 'Shichisai'(♀) and H. syriacus 'Namwon'(♂), which had large flowers over 13 cm diameter and revealed tall tree type. Hibiscus ${\times}$ 'R-142' was selected from the crossing of interspecific hybrid, 'Shichisai'(♀), and H. syriacus 'Namwon'(♂), which had large flowers over 13 cm diameter and revealed tall tree type. The characteristics were succeded after grafting. Flower of 'R-142' had reddish violet color with red eye spot, whereas its parent had blue and purple flowers.