Cellulose Degradation and Extracellulat Enzymatic Activity of the Mud Flat in Sunchon Bay

순천만 갯벌 토양의 섬유소 분해능 및 체외효소 활성

  • Published : 2000.06.01

Abstract

Decomposition rate of organic matiter in the mud flat of Sunchon Bay was estimated. Physicochemical parameters, cellulose degradation rate. distribution of heterotrophic bacteria, and extracellular enzymatic activities were measured from August 1997 to July 1998. Soil temperatures, water contents, concentration of $PO_4$-P and organic matter were -1-~$30^{\circ}C$, 42.1-53.1%, 0.0779-0.1961 mgig and 1.99-7.64%, respectively. Decomposition rate of cellulose film ranged from 7.7 to 100%imonth, high in summer and low in winter. The number of heterotrophic bacteria ranged from $0.87{\times}10^6 to 3.6{\times}10^7 $CUFsIg dq soil. Enzymatic activities of phosphatase, $\alpha$-D-gluEosidase, $\beta$-D-glucosidase and cellobiohydrolase, which were measured as decomposition rate of methylumbelliferyl(MLiF)-substrate, were 152.23-1779.80 nMIhr, 2.67-202.18 nM/hr, 5.03-258.26 M h r and 3.42-63.07 nM/hr, respectively Cellulose degradaaon rate and extracellular extracellular enzymatic activities were conelated with each other, and showed high correlation coefticiency with soil temperature.

순천만 갯벌의 3개 지점의 표층(5cm)과 심층(20 cm)토양의 물리화학적 요인과 종속영양세균의 분포, 섬유소 분해율과 체외효소의 활성을 측정하였다. 온도, 수분함량, 인산염 인 그리고 유기물함량은 각각 -1~$30^{\circ}C$, 42.1~53.1%, 0.0779~0.1961mg/g, 그리고 1.99~7.64%로 나타나었다. 섬유소 films의 분해율은 7.7%~100%/month 범위로 1~2월에 최저 그리고 8~9월에 최고의 분해율을 나타냈다. 종속영양세균의 분포는 $0.87{\times}10^6 ~ 3.6{\times}10^7 $CUFs/g dry soil의 범위에 속해Tdmu 심층의 경우 표층에 비해 낮았다. MUF-기질의 분해율로 측정한 phoshatase, $\alpha$-D-gluocosidase, $\beta$-D-glucosidase, cellobiohydrolase 활성의 변화는 각각 152.23~1779.80 nMi/hr 2.67~202.18 nM/hr, 5.03~258.26 nM/hr, 3.42~63.07 nM/hr으로 모두 하계에 상대적으로 높은 값을 나타내었다. 섬유소 분해는 수온상승이 주된 요인이었으며, 정점간, 깊이간 큰 차이는 나타나지 않았다. 체외효소의 활성은 온도 및 섬유소 분해율과 높은 양의 상관관계를 나타냈다. 종속영양세균의 분포는 섬유소 분해와 체외효소의 활성과 높은 상괸은 나타나지 않았다.

Keywords

References

  1. 순천만의 오염도 및 기초생태계 조사 성치남;박기영;양성렬;김종흥;윤성명.
  2. 한국육수학회지 v.28 낙동강 하구 저질층에서의 혐기성 섬유소 분해에 관한 연구 윤인길;권오섭
  3. 미생물학회지 v.25 금강 하구 퇴적토에서의 종속영양세균의 분포에 미치는 환경요인과 계절적 우점속에 관하여 이견형.
  4. 서남해안 갯벌 생태 조사 보고서 환경부.
  5. Standard methods for the examination of water and wastewater APHA.
  6. Mar. Ecol. Prog. Ser. v.104 Regulation of microbiol enzy-matic degradation of organic matter in deep-sea sediments Boetius, A.;Lochte, K.
  7. Mar. Ecol. Prog. Ser. v.140 Effect of organic enrichments on hydrolytic potentials and growth of bacteria in deep-sea sediments Boetius, A,;Lochte, K.
  8. Limnol. Oceanogr. v.34 Characterization and significance of β-glucosidase activity in lake water Chrost, R.J.
  9. Microbiol. Ecol. v.13 Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in Lake Plussee(North German eutrophic lake) Chrost, R.J.;Overbeck, J.
  10. Mar. Ecol. Prog. Ser. v.108 Storm-stimulated enzymatic decomposition of organic matter in benthic/pelagic coastal meso-cosms Chrost, R.J.;Riemann,B.
  11. Mar. Ecol. prog. Ser. v.11 Significance of exoenzymatic activityes in the ecology of brackish water:measurements by means of methylum-belliferyl substrates Hoppe, H.G.
  12. Mar. Ecol. Prog. Ser. v.93 Evidence for dependency of bacterial growth on enzymatic hydrlysis of particulate organic matter in the mesopelagic ocean Hoppe, H.G.;Ducklow, H.;Karraschm B.
  13. Kor. J. Microbiol. v.23 Distribution and activity of heterotrophic bacteria in the mudflat of Nakdong River Estuary Kim, SJ.;Hong, S.W.;Rhie, Y.;Choi, S.C.
  14. The sediment environment Sediment Microbiology Malcolm, S.T.;Stanley, S.D.;Nedwell, P.B.;Brown, Em.M(Eds.)
  15. Anal. Microbiol. Ecol. v.10 Variability in ectohydrolytic enzyme activities of pelagic marine bacteria and its significance for substrate processing in the sea Martinez, J.;Smith, D.C.;Steward, G.F. Azam, F.
  16. The estuarine ecosystem(2nd ed.) McLusky, D.S.
  17. J. Mar. Res. v.11 The growth and viability of sixty three species of marine bacteria as influenced by hydrostatic pressure. Oppenheimer, C.H.;Zobell,C.E.
  18. American Societty for Testing and Materials. Techniques for investigating the role of anacrobic bacteria in mtehodology for biomass determinations and microbial activities in sediments ASTM STP 673 Parkes, R.J.;Bryder, M.J.;Madden, R.H.;Poole, N.J.
  19. Sourcebook of Experiment for the Teaching of Microbiolgy A rapid colorimetric mthod for estimation of cellulose decomposition by microorganism Swift, J.M.;Primrose, S.B.;Wardlaw, A.C.(ed.)