• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권5호
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• pp.361-366
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• 2009

Purpose: The purpose of this retrospective report was to analyze long-term survival rate of sintered porous-surfaced dental implant ($Endopore^{(R)}$ Dental Implant system, Innova Corporation, Toronto, ON, Canada). Methods: 61 partially edentulous patients were received a total of 127 Endopore dental implants in the maxilla. Of the 127 implants, 24 implants were restored with individual (ie, non-splinted) crowns, while 103 implants were splinted to other implants. Medical records and radiographs were evaluated and analyzed by the cumulative survival rate, location of implants, implants length and diameter, crown/implant ratio and whether the implant was splinted. Chi squire test was used statistically. Result: Of the 127 implants, 8 implants (6.3%) were removed and and cumulative survival rate was 93.7%. Conclusion: Endopore implants showed satisfactory results after up to 8 years function periods in the edentulous posterior maxilla.

• 보건교육건강증진학회지
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• 제29권3호
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• pp.103-114
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• 2012

Objectives: The purpose of this study was to investigate the relationship between mental health status, stress, coping ability, and coping strategies of college students. Methods: The questionnaires were handed out to 162 college students in the Gyeonggi province. Data was collected using the Symptom Checklist-90-Revised (SCL-90-R) scale of the 90 items, the 'Person in the Rain' drawing test, and the 20-item coping strategies questions. Results: 17.3% of the respondents were belonged to the groups of abnormal or abnormal tendency in the mental health status according to the Global Severity Index (GSI) scores. The clinical/border groups were more commonly used in the 'emotion-focused' coping strategies compared to the normal group. This difference was also statistically significant. Both coping resource and coping ability were significantly associated with the mental health status. Conclusions: These findings suggest that future intervention for effective coping strategies should be carefully designed to promote better mental health among college students. It is also important to detect problematic and weak student's behaviors in the early stages.

• Molecules and Cells
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• 제27권1호
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• pp.99-103
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• 2009

Ribose-5-phosphate isomerase A (RpiA) plays an important role in interconverting between ribose-5-phosphate (R5P) and ribulose-5-phosphate in the pentose phosphate pathway and the Calvin cycle. We have determined the crystal structures of the open form RpiA from Vibrio vulnificus YJ106 (VvRpiA) in complex with the R5P and the closed form with arabinose-5-phosphate (A5P) in parallel with the apo VvRpiA at $2.0{\AA}$ resolution. VvRpiA is highly similar to Escherichia coli RpiA, and the VvRpiA-R5P complex strongly resembles the E. coli RpiA-A5P complex. Interestingly, unlike the E. coli RpiA-A5P complex, the position of A5P in the VvRpiA-A5P complex reveals a different position than the R5P binding mode. VvRpiA-A5P has a sugar ring inside the binding pocket and a phosphate group outside the binding pocket: By contrast, the sugar ring of A5P interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues; the phosphate group of A5P interacts with two water molecules, W51 and W82.

• 동의신경정신과학회지
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• 제23권3호
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• pp.23-32
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• 2012

Objectives : The purpose of this study was to validate the Sasang Personality Questionnaire (SPQ), based on the diagnosis of Sasang constitutional medicine clinical specialist. Methods : We performed Sasang type differentiation of 103 college students by certified specialist, and analyzed biopsychological traits of each groups with SPQ, Temperament and Character Inventory (TCI), Positive and Negative Affection Schedule (PANAS) and Body Mass Index (BMI). Results : The SPQ score of So-Yang ($33.1{\pm}0.79$), Tae-Eum ($29.03{\pm}0.67$) and So-Eum ($25.76{\pm}0.98$) Sasang groups showed significant differences from each other. The SPQ showed significant correlation with TCI Novelty-Seeking (r=.346), TCI Harm-Avoidance (r=-.348) and TCI Persistence (r=.346). But the BMI and PANAS did not show significant correlation with SPQ, although the PANAS Positive Affection (r=.332) has significant correlation with SPQ-Behavior. Conclusions : We confirmed the clinical validity of SPQ with clinical specialist and established biopsychosocial instruments. This study would contribute to a more objective diagnosis and studies on biopsychosocial mechanism of Sasang constitutional medicine.

• 대한화장품학회지
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• 제32권2호
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• pp.99-103
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• 2006

티트리오일 2종과 편백나무에서 추출한 천연오일을 합성방부제인 methylparaben과 항균력을 비교하였다. 대상 균주로 그람양성균인 S. aureus (KCTC 1916)와 그람음성균인 E. coli (KCTC 2571)와 P. aeruginosa (KCTC 2513)를 사용하였으며, 진균류인 C. albicans (KCTC 7965)와 A. niger (KCTC 6911), 그리고 여드름균인 P. acnes (KCTC 3314)를 사용하였다. 그람양성균의 항균력실험에서 천연오일은 합성방부제보다 우수한 항균활성을 보였으며, 그람음성균에서는 유사한 정도의 활성을 나타내었다. 특히, 진균과 여드름균에 대해서는 합성물질에 비해 천연오일에서 월등한 활성을 볼 수 있었다. 실험군에서 항균력은 티트리오일 > methylparaben > 편백정유의 순으로 나타났다. 또한, DPPH 법에 의한 항산화실험과 lipoxygenase를 이용한 항염증실험을 통해 천연오일의 항산화 및 항염증 효과를 볼 수 있었다. 실험결과, 천연오일은 널리 사용되고 있는 화학적인 방부제나 항산화제를 충분히 대체할 수 있으리라 기대된다.

• 대한의생명과학회지
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• 제25권1호
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• pp.75-82
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• 2019

We developed the multiplex LAMP assay using 16S rRNA, femA and mecA genes for direct detection of the methicillin resistance in Staphylococci from positive blood culture. To simultaneously recognize Staphylococci genus, S. aureus and methicillin resistance, three sets of six primers for 16S rRNA, femA and mecA were designed, respectively. The performance of LAMP assay was affirmed using VITEK system for the phenotypic methods of identification and for oxacillin and cefoxitin antimicrobial susceptibility. The optimal condition for LAMP assay was obtained under $64^{\circ}C$ for 50 min. The detection limit was determined to be of 20 copies and CFU/reaction ($10^4CFU/mL$). For clinical application of comparison with phenotypic methods, the sensitivity and specificity of the LAMP with femA gene for detecting S. aureus was 95.31% and 100%, respectively. The sensitivity and specificity of the LAMP with mecA gene for detecting methicillin resistance was 98.46% and 100%, respectively. The multiplex LAMP assay with femA and mecA gene successfully detected all of MRSA (38 isolates) isolates from 103 Staphylococci in blood cultures. The LAMP assay developed in this study is sensitive, specific, and of excellent agreement with the phenotypic methods.

• 한국수산과학회지
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• 제33권2호
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• pp.103-109
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• 2000

열목어를 비롯한 산천어, 시마연어, 연어, 무지개송어 등 우리나라 연어아과 어류의 집단구조분석을 위한 기초자료를 얻기 위하여, 미토콘드리아 ribosomal RMA 유전자 영역의 염기서열변이를 비교${\cdot}$분석하였다. 미토콘드리아 DNA의 125 rRNA(945 bases, 열목어 의 경우 946 bases), Valine transfer RNA (72 bases), 및 16S rRNA(1513 bases) 등 3개의 유전자 영역에 걸쳐, 최대 2531 bases의 염기서열을 PCR/direct sequencing하여 얻었는데, 모든 염기변이중 전이가 월등히 우세하게 나타났으며, 종내${\cdot}$종간변이율은 모두 $0.5{\%}$이하로 낮게 나타나, 다른 영역에 비해 rRNA 유전자 영역에서의 염기서열이 매우 보존적임을 보여주었다. 또한, 미토콘드리아 rRNA 유전자 염기서열은 연어류의 속 (genus)단계 이상에서 집단분류표지인자로 유용하게 쓰일 수 있으리라 사료되어진다. 미토콘드리아 rRNA 염기서열자료를 기초로 구성된 phylogenetic tree를 통해 이들 종간의 진화적인 유연관계를 살펴본 결과, 시마연어가 무지개송어보다는 연어와 더 근연인 것으로 나타났으며, 열목어는 가장 유연이 먼 종임을 확인할 수 있었다.

• 대한의생명과학회지
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• 제7권3호
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• pp.103-110
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• 2001

$\alpha$-Fetoprotein(AFP) is a good marker for the detection of several diseases such as hepatocellular carcinoma, gonadal germ cell tumor, gastric tumor, and Down's syndrome. In this study, we developed ELISA, using synthetic peptides corresponding to the epitopes of AFP. Five kinds of peptides were synthesized from AFP to produce antibodies in rats that recognize AFP in human plasma as well as amniotic fluid and do not cross-react with serum albumin. All five kinds of antibodies showed good reactivities with their peptide-keyhole limpet hemocyanin conjugates. Anti-synthetic peptide 1 (R-N-E-Y-G-I-A-S-I-L, 4-13) antibody, in particular, reacted well with AEP as well as synthetic peptide 1-KLH but not with human serum albumin. The binding affinity(Kd) was 2.7$\times$10$^{-9}$M for peptide 1 and 6.8$\times$10$^{-8}$M for AEP. The range for measurement of AFP was 10~1,000 ng/ml. The within-assay and between-assay coefficients of variance(CV) were 4.83% and 10.97%, respectively. In a sample of 31 sera and 33 amniotic fluids, there was a good correlation between AFP values determined in this assay and those in a commercial kit. These results indicate that the antibodies against synthetic peptides corresponding to the epitopes of AFP are highly specific to APP and synthetic peptide-based ELISA would be useful for the measurement of human AFP.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2003년도 학술대회지
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• pp.97-103
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• 2003

Phytochrome controls diverse aspects of plant development in response to the ambient light conditions. HFRl, a basic helix-loop-helix protein, is required for a subset of phytochrome A (phy A)-mediated photo-responses in Arabidopsis. Here, we show that overexpression of HFR1-N105, but not the one of the full-length HFR1, confers exaggerated photo-responses. The transgenic plants overexpressing HFR1- N105 exhibited light-independence in a subset of photo-responses, including germination, de-etiolation, gravitropic hypocotyl growth, and blocking of greening. Overexpression of HFR1-N105 also caused constitutive light-responses in the expression of some light-regulated genes. In addition, the HFR1-N105 overexpressor showed hypersensitive responses under R and FR light, dependently on phyB and phyA, respectively. End-of-day far-red light response and petiole elongation were suppressed in the HFR1-N105 overexpressor plants. Together these results imply that overexpression of HFR1-N105 activated a branch of light signaling, supporting the hypothesis that transcriptional regulation in the nucleus would be the primary mechanism of light signaling in Arabidopsis. We discuss the biotechnological potential of the mutant bHLH protein, HFR1-N105 in regard to suppressed shade avoidance syndrome.

• 한국방재학회 논문집
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• 제9권3호
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• pp.59-65
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• 2009

본 연구에서는 소방공무원의 직무형태별 외상 후 스트레스와 사회적지지 및 업무부담감을 알아보고 직무별 고위험군을 확인 하고 외상 후 스트레스에 영향을 주는 변수들을 알아보고자 하였다. 자료는 C도에 근무하는 소방대원 중 구급 96명, 구조 및 화재진압 85명, 기관 83명인 총 264 명이 수집되었다. SPSS WIN 14.0 program을 이용하여 총 22문항으로 구성된 외상 후 스트레스 검사 도구 (IES-R), 20문항으로 최은숙(2000)이 수정하여 개발한 업무 부담감 측정도구와 오진환(2006)의 연구에서 수정보완 한 사회적지지도구로 분석하였다. 직무별 일반적 특성에 따른 외상 후 스트레스증상의 결과는 나이, 결혼, 학력, 직급, 재직기간이 세 직무집단 간에서 유의한 차이를 보였다. 직무별 외상 후 스트레스는 구조 및 화재 진압에서 가장 높았으며 세집단 간에 유의한 차이를 보였다. 직무별 세 변수와의 상관관계에서 구조 직무군에서 업무부담감(r=.317, p<0.01)이 많을수록 외상 후 스트레스는 높았고, 구급 직무군에서는 사회적 지지(r=-.331, p<0.01)가 낮을수록, 업무부담감(r=.522 p<0.001)이 많을 수록 외상 후 스트레스가 높았고, 기관 직무군에서는 업무부담감(r=.454, p<0.01)이 많을수록 외상 후 스트레스가 높게 나타났다. 고위험군에 속한 군은 구조 및 화재 진압 군에서는 51명(60%) 구급은 53명(55.2%), 기관은 38명(45.8%)으로 전체 264명 중 142명(54%)이었고 직무별 외상 후 스트레스 고위험군에 영향 주는 변수로는 구조 및 화재 진압군은 요구조자 위험을 경험시 느끼는 충격이었으며(odds ratio=1.216, 95% 신뢰구간: 1.068-1.383) 구급직무군은 업무부담감과(odds ratio=1.100, 95% 신뢰구간: 1.043-1.159) 재직기간(odds ratio= 1.010, 95% 신뢰구간 1.001-1.018)으로 나타났고 기관 직무군에서는 요구조자의 위험을 경험시 느끼는 충격(odds ratio=1.178, 95% 신뢰구간: 1.010-1.373), 나이(odds ratio=1.129, 95% 신뢰구간: 1.020-1.249), 업무부담감(odds ratio= 1.103, 95% 신뢰구간: 1.034-1.177)이었다.