• Microbiology and Biotechnology Letters
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• v.5 no.3
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• pp.141-151
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• 1977

Distribution of tellurite and tellurate-reducing enzymes in the cell of Nocardia sp, the purifcation and the chemical properties of enzymes were investigated. Tellurite- and tellurate-reducing enzymes were located in the cytoplasm, but T. T. C. reduction part was in the cell membrane. Purification of tellurite- and tellurate-reducing enzymes was possible with the application of ammonium sulfate precipitation method and DEAE-Cellulose or CM-Cellulose column chromatographic method from the crude soluble part of the cell. On investigating the properties of purified enzyme, one of NADP, NADPH and reductive methylene blue(leucomethylene blue) was thought to react as a hydrogen donor. Both NADH and NADPH, or either of them would be physiological hydrogen donor.) In the reaction of this enzyme, either tellurite or tellurate reacts as a hydrogen acceptor, but on the other hand either selenate or selenate also reacts as a hydrogen acceptor.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.416-422
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• 2003

Jaeumgenby-tang(JGT) have been used in oriental medicine for many centuries as a therapeutic agent of vertigo caused by deficiency of qi(氣) and blood(血). Effect of Aurantii Fructus(AF) take off the phlegm by promoting the circulation of qi, Gastrodae Rhizoma(GR) has effects treating for headache, vertigo by calming the liver and suppressing hyperactivity of the liver-yang(陽). And, We reported that JGT adding AFㆍGR extract(JGTAG) was significantly increased regional cerebral blood f1ow(rCBF) by dilating pial arterial diameter(PAD). Therefor we designed to investigate whether JGTAG is mediated by adrenergic β-receptor, cyclooxygenase or guanylate cyclase in normal rats. The changes of rCBF and mean arterial blood pressure(MABP) were determinated by laser-doppler flowmetry(LDF), and the change of PAD was determinated by video microscope and width analyzer. The results were as follows in normal rats; Pretreatment with propranolol(1mg/kg, i.v.) was significantly inhibited JGTAG induced increase of rCBF, PAD and MABP, and pretreatment with indomethacin(1 mg/kg, i.v.) was significantly inhibited too. But pretreatment with methylene blue(10μg/kg, i.v.) were accelerated JGTAG induced increase of rCBF and MABP, but pretreatment with methylene blue was inhibited JGTAG induced increase of PAD. This results suggest that the mechanism of JGTAG is mediated by adrenergic β-receptor and cyclooxygenase.

• The Korean Journal of Pharmacology
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• v.26 no.1
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• pp.13-23
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• 1990

Methylene Blue (MeB) and gentian violet $(10^{-6}{\sim}10^{-4}\;M)$ produced contractions in isolated thoracic aortic preparations of rabbits in a dose-dependent fashion, while other dyes, evans blue and eosine yellowish, did not affect the basal tension in the same range of doses. Porcine mesenteric arterial rings also responded to MeB with dose-dependent contractions. Single dose of $10^{-4}$ M MeB produced a biphasic response: contraction followed by relaxation. The contraction developed slowly within $2{\sim}4$ min and peaked in about 20 minutes and then slowly relaxed to the basal level. Tyramine $(10^{-4}\;M)$ also induced contraction but it developed faster and was more persistent than that of MeB. While the tyramine-induced tension was reproducible, the MeB-induced one wat not reiterable until 3 to 5 hours after washing out the MeB. Adding $10^{-4}$ M MeB further potentiated the contraction induced by $10^{-4}$ M tyramine. However, the MeB contraction was not affected by further addition or tyramine. Both tyramine- and MeB-induced tensions were abolished or significantly inhibited by pretreatment with various drugs acting on the sympathetic nervous system. The tyramine-induced tension was more sensitive to guanethidine and 6-hydroxydopamine than the MeB-induced tension, while the latter was more sensitive to $Ca^{2+}-free$ PSS and reserpine. But they have similar sensitivity to prazosin. The MeB-induced tension was significantly inhibited but not abolished by 6-hydroxydopamine pretreatment. However, either tyramine or 6-hydroxydopamine could not affect the basal tension of the ring that MeB once had been tested. These results suggest that MeB-induced contractions of rabbit thoracic aorta and porcine mesenteric artery result from a release of endogenous norepinephrine from adrenergic nerve endings and are dependent in part on extracellular calcium, and that the potency of MeB to release or to deplete norepinephrine is greater than that of either tyramine or 6-hydroxydopamine.

• Applied Chemistry for Engineering
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• v.20 no.3
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• pp.329-334
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• 2009

Yttrium ions doped $TiO_2$ particles have been prepared using a low temperature combustion method. The physical properties were investigated, together with the activity of $TiO_2$ particles as a photocatalyst for the decomposition of methylene blue. From XRD results, the major phase of all the $TiO_2$ particles prepared under basic condition was an anatase structure but a rutile peak was observed when they are prepared under acidic condition. The crystallite size of $TiO_2$ particles was decreased as the molar ratio of CA/TTIP increased. The photocatalytic activity increased with an increase of CA/TTIP molar ratio and pH in the solution. In addition, the doping of 1.0 mole% yttrium ion on the $TiO_2$ enhanced the photocatalytic activity and showed the higher activity than commercial P-25 catalyst.

• Parasites, Hosts and Diseases
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• v.54 no.2
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• pp.191-195
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• 2016

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1714-1721
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• 2004

This experimental study was designed to investigate the mechanism of Palmul-Tang(PMT) on the changes of cerebral hemodynamics in rats. The changes of cerebral hemodynamics in normal rats were as follows ; The PMT-induced increase in regional cerebral blood flow was significantly inhibited by pretreatment with indomethacin(1㎎/㎏, i.p.), an inhibitor of cyclooxygenase, and was inhibited by methylene blue(10㎍/㎏, i.p.), an inhibitor of guanylate cyclase. The PMT-induced dilation in pial arterial diameter was significantly inhibited by pretreatment with indomethacin and methylene blue. The PMT-induced increase in mean arterial blood pressure was significantly inhibited by pretreatment with indomethacin but was increased by methylene blue. This results were suggested that the mechanism of PMT was mediated by cyclooxygenase. The changes of cytokine production in cerebral ischemic rats were as follows ; In cytokine production of serum by drawing from femoral arterial blood after middle cerebral arterial occlusion 1hr, sample group was decreased IL-1β and TNF-α production compared with control group, IL-10 production of sample group was similar to that of control group, but sample group was significantly increased TGF-β production compared with control group. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 1hr, sample group was significantly decreased IL-1β production compared with control group and decreased TNF-α production compared with control group. IL-10 production of sample group was similar to that of control group, but sample group was significantly increased TGF-β production compared with control group. In cytokine production of serum by drawing from femoral arterial blood after reperfusion 4 hrs, sample group was significantly decreased IL-1β production compared with control group, but IL-10 production of sample group was similar to that of control group. sample group was increased TNF-α and TGF-β production compared with control group. These results suggested that PMT had inhibitive effect on the brain damage by inhibiting IL-1β and TNF-α production, but by accelerating TGF-β production. The present author thought that PMT had an anti-ischemic effect through the improvement of cerebral hemodynamics and inhibitive effect on the brain damage.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.9
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• pp.900-906
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• 2008

It was studied to utilize wasted food as a starting material to produce for activated carbon. The wasted food was chemically activated with zinc chloride. Experiments were carried out at different chemical ratios(activating agent/wasted foods), activation temperatures, and activation time. The activated products were characterized by measuring the iodine and methylene blue number, the BET surface area, the pore volume, the micropore ratio, the pore diameter, the yields and the scanning electron microscope(SEM). For the products activated by impregnation ratio of 1.0 of ZnCl$_2$ at 500$^{\circ}C$ for 60 min in a rotary kiln reactor had iodine number of 480 mg/g, methylene blue number of 95 mL/g, BET surface area of 410 m$^2$/g, pore volume of 0.248 cm$^3$/g, and average pore diameter of 2.43 nm, respectively. The activated carbon obtained had the contribution of micropore area of 70.7% to the total pore area and micropore volume of 53.2% to the total pore volume.

• Journal of the Korea Concrete Institute
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• v.25 no.3
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• pp.347-354
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• 2013

Recently, crushed fine aggregates are being widely used due to the shortage of natural sand. In Korea, the amount of fine particles under 0.08 mm contained in crushed fine aggregates is restricted to be less than 7%, which is similar to the regulations of ASTM but is still very strict compared to the regulations of the other nations. In addition, the crushed aggregates already have in them about 20% of fine particles under 0.08 mm which occurs while they are crushed. The fine particles are not easy to wash out, and also to maximize the use of resources it is deemed necessary to review the possibility of enhancing the limit of the amount of fine particles. Therefore, this study conducted experiments to analyze the characteristics of fine particles under 0.08mm and their influence on the properties of concrete. Experiments using silt and cohesive soil were also done for comparison. In the experiments on fine particles, the methylene blue value was more in the soil dust contained in silt and cohesive soil than in the stone powder contained in crushed fine aggregates. Also, the methylene blue value had a close correlation with packing density and liquid & plastic limit. In the experiments done with concrete, the quantity of high range water reducing agent demanded to obtain the same slump increased as the fine particle substitution rate heightened. However, in the experiment which used stone powder testing the compressive strength and tensile strength of concrete in the same water-cement ratio, there was little change in strength with less than 20% addition of fine particles among the fine aggregates, and no meaningful difference in the amount of drying shrinkage of concrete.

• Food Science and Preservation
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• v.8 no.3
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• pp.326-330
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• 2001

The characteristics of used wine yeast strains were as follows, S6U showed low fermentation speed than those of other yeast strains, but this strain fermented completely later. The wine fermented by W-3 was very low contents of total acid, 0.75% and the ones fermented by UCD530 and AC- contained much extract, 3.26 mg/L and 3.22 mg/L respectively. The wine fermented by CEG and CS2 were predominant in yellowness, and EC1118 produced large amount of acetaldehyde, 49.9 mg/L than those of other strains. EC1118 and CY3079 displayed low methylene blue dyeing ratio, below 15%, meaning high alcohol tolerance yeast. UCD530 produced extremely high contents of glycerol, succinate and lactate compared with other strains. These properties revealed that UCD530 was a typical Saccharomyces bayanus species. The main organic acids produced by wine yeasts were pyruvate, lactate, succinate and acetate. The concentration of acetate in experimental wine could be divided into two parts, one group had concentration below 170 mg/L (UCD530, EC1118, AC-, CY3079, W-3), and the other had concentration up to 350 mg/L (S6U, CEG, CS2).

• The Journal of Korean Medicine
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• v.26 no.3 s.63
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• pp.188-203
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• 2005

Objectives : This study was designed to investigate the effects of Jayun-tang extract (JYT) on the change of cerebral hemodynamics [regional cerebral blood flow (rCBF), pial arterial diameter (PAD) and mean arterial blood pressure (MABP)] in normal and cerebral ischemic rats, na to determine the mechanisms of action of JYT. Methods : We investigated whether JYT inhibits lactate dehydrogenase activity in neuronal cells and cytokines production in serum of cerebral ischemic rats. Results : 1. JYT significantly increased rCBF and PAD in a dose-dependent manner, but MABP was not changed by injecting JYT. These results suggested JYT significantly increased rCBF by dilating PAD. 2. The JYT-induced increase in rCBF was significantly inhibited from pretreatment with indomethacin (1mg/kg, i.p.), an inhibitor of cyclooxygenase and methylene blue $(10{\mu}g/kg, i.p.)$, an inhibitor of guanylate cyclase. 3. The JYT-induced dilation in PAD was significantly inhibited from pretreatment with indomethacin, but was increased by pretreatment with methylene blue. 4 The JYT-induced increase in MABP was reduced by pretreatment with indomethacin and methylene blue. 5. JYT significantly inhibited lactate dehydrogenase activity in neuronal cells. These results suggest that JYT prevented the neuronal death. 6. Both rCBF and PAD were significantly and stably increased by JYT $(10{\mu}g/kg,\;i.p.)$ during the Period or cerebral reperfusion, which contrasted with the findings of rapid and marked increase in the control group. 7. In cytokine production in the serum drawn from femoral artery 1hr after middle cerebral artery occlusion, the sample group showed significantly decreased production of $IL-1\beta$ and $TNF-\alpha$ as well as increased production of IL-10 and $TGF-\beta$ compared with rho control group. 8. In cytokine production in the serum drawn from femoral artery 1hr after reperfusion, the sample group showed significantly decreased production of $IL-1\beta$ and $TNF-\alpha$ as well as significantly increased production of IL-10 and $TGF-\beta$ compared with the control group. Conclusions : JYT mediated by cyclooxygenase had an inhibitive effect on brain damage by inhibiting lactate dehydrogenase activity, $IL-1\beta$ and $TNF-\alpha$ production, and by accelerating IL-10 and $TGF-\beta$ production. The author feels that JYT had anti-ischemic effects through the improvement of cerebral hemodynamics and inhibitive effects on brain damage.