• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.1
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• pp.23-33
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• 2007

Classification results of rural villages provide useful information about rural village characteristics to select similar villages in rural development project; many researches about regional classification have been practiced. Recently rural amenity was introduced as an alternative for rural development, and rural villages have been surveyed to find potential resources for rural development by 'Rural Amenity Resources Survey Project'. Accumulated information through this survey project could be used to classify rural villages. However existing rural classification method using statistical data is not efficient method to use rural amenity resources information described with text. We introduced Information Bottleneck Method (IBM) based on information theory and implemented this method to classification with rural amenity resources information of Yanggang-myen, Yeongdong-gun in Chungbuk province.

• IE interfaces
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• v.24 no.4
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• pp.420-427
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• 2011

The present study developed an anthropometric design method accommodating physical human variabilities for user-centered product development. The proposed design method is based on the boundary zone method, a technique to generate a group of humanoids properly representing the body size diversity of thedesign target population. In addition, the anthropometric design method considers the variability of postures in the design process by incorporating the simulation of posture. The effectiveness of the proposed design method was evaluated in terms of multivariate accommodation percentage (MAP) by applying it to designing a computer workstation with 90% of accommodation percentage. The performance evaluation showed that the MAP (89%) of the computer workstation design produced by the proposed method was quite close to the designated accommodation percentage. The proposed design method can be of use to develop an effective anthropometric design for user-centered product development.

• Journal of The Korean Digital Architecture Interior Association
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• v.13 no.3
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• pp.5-14
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• 2013

In recent years, the change of urban function and the decrease in birth rate have given rise to merge, abolition and relocation of the existing schools. This study explores the possibilities for various land utilizations of disused school sites, aims to propose the effective asset management and the proper development methodology. Firstly it examines the status of small class school in urban area and the linkage complex development method. Among various methods, it compares the validity between the closed school management and public asset management method. In addition, it analyses the national finance method and public-private partnership investment as a proper development methodology to ensure both the common-benefit and profitable business. As a result of study, it prposes the BTO(build-transfer-operate) method to use effectively the disused school sites in urban area.

• Journal of the Korean Society of Costume
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• v.66 no.4
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• pp.61-76
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• 2016

The importance of environment has come into focus recently, and this has led to increased attention on zero waste fashion design as a method to minimize waste from the production stage of fashion goods. The purpose of this study was to analyze the development method types and the characteristics of zero waste fashion design in order to study the eco-friendly meanings of zero waste fashion design, as well as its meaning as creative design development methods. Through the case analysis of recent designs, the design types of zero waste fashion design were largely classified into cut and sew, folding, draping, and non-woven types, and they were classified again according to the characteristics of production process. According to the result of analyzing fashion design development methods of zero waste fashion designs based on the process of completing design, they were classified into pattern making, computer programming, draping, assembling of the unit, and non-woven moulding methods, and the aspect of combined use rather than utilization of one method appeared. Formative characteristics of zero waste fashion design included decorative beauty, formative beauty, and transformable beauty and its design characteristics were fortuity and unexpected properties, breaking stereotypes, structural flexibility and futuristic innovation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.47 no.1
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• pp.75-80
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• 2019

In the recent aerospace and defence industries, it is required to develop small and low cost embedded systems. Based on a high speed digital signal processor (DSP), this paper first presents the development of an embedded system. To reduce the computation time of the high precision algorithm such as flight control, we also propose two algorithms for matrix multiplication. Validation results show that, compared to the performance using the $2{\times}2$ unit method, the performance of the proposed method 1 is improved, when the size of matrices is small. The proposed method 2 generally outperforms the $2{\times}2$ unit method.

• Journal of Information Technology Services
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• v.20 no.1
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• pp.41-54
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• 2021

The SW-centered battlefield management information system R&D project takes a long period of 5-10 years or more by applying a complex and rigid batch acquisition strategy. In order to solve this problem, it is necessary to institutionalize a rapid and flexible battlefield management information system R&D project management procedure applying agile development methodology, and a government project management organization and contract management method to support it In this study, we analyzed the case of applying the Agile development method centered on Scrum to the US SW-centered weapon system R&D project and the characteristics and problems of the battlefield management information system R&D project in Korea, and suggested improvement measures as follows. First, the battlefield management information system R&D model applies the hybrid development method, and the system requirements analysis and system structure design use the existing waterfall development procedure, and the agile method is applied from the SW requirements analysis to the system integration stage. Second, flexible adjustment of performance, schedule, and cost by organizing an Agile IPT in which military (requirements) - DAPA (project management) - developer - functional specialized organizations (test and evaluation, quality, government research institutes, etc.) participate. Third, improving the Basic Order Agreement so that it can be applied to agile R&D.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.2
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• pp.78-84
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• 2006

A high performance liquid chromatography assay method for spiramycin in fish muscle was developed. The developed method was evaluated and validated by monitoring spiramycin in olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli) and in live conger eel (Anguilla japonica) in fish farms and distribution centers. Using the developed method, the recovery rate was up to 82.4-88.8%, which was higher than that of conventional methods (77.6-87.1%). In particular, the proposed sample treatment protocol was suitable for use with fish samples to remove low molecular weight materials and pigments that could interfere an accurate analysis. The prepared stock solution was very stable, and it remained chemically stable for 5 weeks at $4^{\circ}C$. The performance limit of the developed method for spiramycin acid in fish muscle was 0.05 ppm. One hundred thirty-four fish samples including olive flounder, black rock fish and live conger eel were analyzed to evaluate the overall efficiency of the modified method and to monitor the actual condition of spiramycin usage in fish farms. Olive flounder and black rock fish were collected from fish farms in coastal areas of Korea, and live conger eels were purchased from a fish market in the Busan area from September 2001 to March 2002. According to the overall performance of the developed method, it was considered suitable for the monitoring of spiramycin in fish muscle. The suggested method of analysis for spiramycin in fish muscle is registered in the Korean Official Methods of Food Analysis and is currently tieing utilized for fishery products inspection by the Korea Food and Drug Administration and the National Fisheries Products Quality Inspection Service.

• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.25-30
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• 2015

Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.

• Korean Journal of Medicinal Crop Science
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• v.22 no.1
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• pp.17-22
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• 2014

Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial least squares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding and transplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding and transplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting method were relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples produced from direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identification of ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between direct and transplanting methods.

• Journal of Animal Science and Technology
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• v.66 no.5
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• pp.1021-1033
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• 2024

Exosomes have been extensively studied as disease biomarker in humans, given their role in transporting bioactive molecules. However, despite the great potential of exosomes as noninvasive diagnostic markers and therapeutic nanocarriers for bovine diseases, few studies have been conducted on bovine exosome. Thus, this study aimed to quantitatively and qualitatively compare three isolation methods to identify a suitable method for bovine serum. Exosomes were isolated using ultracentrifugation alone (UC), a combination of ultracentrifugation and size exclusion chromatography (US), or membrane affinity-based exoEasy kit (EE). Isolated particles were evaluated using a range of complementary techniques. Transmission electron microscopy showed that all three isolation methods resulted in particles with a cup-shaped morphology. The particle concentration measured by nanoparticle trafficking analyzer of US was lower compared to those of UC and EE method. As a result of immunoblotting, exosome markers including TSG101, CD81, and HSP70 were detected in US particles, while in UC and EE, only TSG101 expression was confirmed. Particles isolated from UC and EE showed a contamination with the blood protein albumin, whereas particles from US did not show albumin contamination. In addition, to evaluate the possibility of using exosomes as biomarkers, the profiles of the small RNA in the exosomes were compared using the bioanalyzer 2100. As a result, in the EE method, the band of small RNA (25-200 nt) was most prominent, and in the US methods, a distinct band was observed in the small RNA range. Collectively, the purity of exosomes without non-exosomal contamination was highest in the US method. However, for the detection of small RNA, the EE method was found to be the most suitable. Therefore, the results suggest that the optimal isolation method varies depending on the specific purpose of exosome isolation.