• Journal of Applied Biological Chemistry
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• 제58권4호
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• pp.383-387
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• 2015

국내 유통 식품 수입 식품 중 토끼와 고양이 고기의 혼입 여부를 알아내고 불법 도축된 고양이 고기를 토끼 고기나 다른 고기로 속여 판매하는 것을 방지하기 위해 토끼와 고양이를 동시에 검출할 수 있는 polymerase chain reaction (PCR) 법을 개발하였다. 토끼와 고양이의 종 특이 프라이머는 미토콘드리아의 cytochrome b 유전자를 대상으로 하였고 개발된 프라이머를 가공식품에 활용하는 것을 고려하여 PCR 산물의 크기는 토끼 101 bp, 고양이 191 bp로 최소화 하였다. 프라이머의 특이성은 총 21종의 동물을 대상으로 검토하였다. 개발된 검출법의 검출 한계는 시료 DNA를 희석하여 PCR과 Bioanalyzer로 확인한 결과 토끼는 0.005 ng, 고양이는 0.0005 ng이었다.

• 한국가시화정보학회지
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• 제20권3호
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• pp.136-145
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• 2022

Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 10¹² copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.

• 대한환경공학회지
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• 제37권2호
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• pp.81-86
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• 2015

수중에 존재하는 포름알데하이드(formaldehyde)를 ${\mu}g/L$ 단위 농도까지 정량할 수 있는 간단한 분석법을 개발하였다. 시료 전처리시 유도체화 시약으로 아세틸아세톤(acetylacetone)을 사용하였고, 기존 분석방법과 달리 추출 및 농축과정을 거치지 않는 것이 장점이다. 시료에 유도체화 시약을 첨가하고 $80^{\circ}C$에서 30분 반응시킨 후 HPLC를 이용하여 포름알데하이드를 분석하였다. 본 방법에 대한 정도보증 결과, 방법검출한계 및 정량한계는 각각 1.6, $5.0{\mu}g/L$로 기존 분석법에 비하여 낮았다. 정밀도 및 정확도는 각각 0.6~3.0%, 91.6~106.3%로 우수하였다. 또한 다양한 환경시료에 대한 회수율 검증에서도 92.0~115.2%로 양호하게 나타났다.

• 한국식품위생안전성학회지
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• 제18권2호
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• pp.43-50
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• 2003

A liquid chromatographic method, using matrix solid-phase dispersion (MSPD) is developed for the extraction of residual furazolidone in chicken eggs. Blank or fortified egg samples (0.5 g) were blended with Octadecylsilyl (Bulk $C_{18}$, 40${\mu}{\textrm}{m}$, 18%. load, endcapped. 2 g) derivatized silica. After homogenization, $C_{18}$/egg and Na$_2$S $O_4$matrix were transferred to a column made of 10 ml glass syringe and filter paper and compressed 4.0∼4.5 ml volume. The column was washed with 8 ml of hexane and dried under $N_2$ gas. Furazolidone was eluted with acetonitrile (8 ml) under gravity. The eluate containing furazolidone was free from interfering compounds when analyzed by HPLC with UV detection (365 nm, photodiode array). Calibration curves were linear (r = 0.99985) and inter- (1.47%) and intra-assay (5.29%) variabilities for the concentration range examined (7.8∼497 ng/g of eggs, 20 ${mu}ell$ injection volume) were indicative of an acceptable methodology for the analysis of furazolidone. Average recovery of furazolidone added to egg was 96.2%. The limit of detection for the proposed method was 1 ng/g for furazolidone. The method using MSPD is proposed as an alternative assay to the classical method which involves the use of large volumes of a harmful solvent and requires a long tedious separation and clean-up processes prior to its determination.

• Archives of Pharmacal Research
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• 제29권9호
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• pp.808-813
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• 2006

Two methods for the chiral purity determination of bevantolol were developed, namely capillary electrophoresis (CE) using carboxymethyl-${\beta}$-cyclodextrin (CM-${\beta}$-CD) as a chiral selector and high-perfomance liquid chromatography (HPLC) using a chiral stationary phase. In the HPLC method, the separation of bevantolol enantiomers was performed on a Chiralpak AD-H column by isocratic elution with n-hexane-ethanol-diethylamine (10:90:0.1, v/v/v) as mobile phase. In the CE method, bevantolol enantiomers were separated on an uncoated fused silica capillary with 50 mM amonium phosphate dibasic adjusted to a pH 6.5 with phosphoric acid containing 15 mM CM-${\beta}$-CD as running buffer. Validation data such as linearity, recovery, detection limit, and precision of the two methods are presented. The detection limits of S-(-)-bevantolol were 0.1% and 0.05% for CE and HPLC method, respectively and R-(+)-bevantolol were 0.15% and 0.05% for CE and HPLC method, respectively. There was generally good agreement between the HPLC and CE results.

• 약학회지
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• 제56권3호
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• pp.145-151
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• 2012

To develop fundamental data of herbal materials with heat treatment, we studied analytical and extraction methods of benzopyrene in black ginseng and validated by HPLC analysis. Benzopyrene was successfully separated in mobile phase of acetonitrile:water (80 : 20) and detection of excitation 294 nm, emission 404 nm. The calibration curve of benzopyrene was linear over the concentration range of 1.17~37.50 ng/ml with correlation coefficient of above 0.999. The limit of detection (LOD) and quantitation (LOQ) of benzopyrene was 0.25 and 0.75 ng/ml, respectively. Hexane extraction method was used as a new extraction method for benzopyren and the efficient of extraction was over 95%. In conclusion, analytical method and extraction method were suitable for the determination of benzopyrene in the black ginseng and could be applied to fundamental study and guideline of herbal materials with heat treatment.

• Journal of Pharmaceutical Investigation
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• 제34권4호
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• pp.327-331
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• 2004

A simple HPLC method with ultraviolet detection of ciprofloxacin in human plasma was developed and validated. After protein precipitation with trichloroacetic acid, chromatographic separation of ciprofloxacin in plasma was achieved at $50^{\circ}C$ with a $C_{18}$ column and methanol-phosphate mixture (pH 2.5), as mobile phase. Quantitative determination was performed by ultraviolet detection at 278 nm. The method was specific and validated with a limit of quantification of 100 ng/ml. The intra- and inter-day coefficients of variation were between 1.67% and 10.55% and accuracy between 92.01 % and 106.09%. The method has been successfully applied in a bioavailability study of 250 mg ciprofloxacin hydrochloride tablet.

• 대한수의학회지
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• 제43권3호
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• pp.435-438
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• 2003

The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.

• 한국식품과학회지
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• 제40권4호
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• pp.375-381
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• 2008

식품에 사용이 금지된 허용 외 색소 중 Orange II, Rhodamine B, Para red, Sudan I-IV 색소의 효율적인 수입식품 안전관리와 수용성 착색료 및 지용성 착색료로 이원화되어 있는 허용 외 착색료의 신속하고 정확한 분석을 위하여 동시 분석법을 개발하고자 하였다. 문헌조사를 바탕으로 분석을 위한 컬럼, 이동상 조건, 분석파장, 전처리 조건 등을 검토하여 분석법을 개발하였으며, 개발된 분석법의 검출한계 설정, 유효성 검증 및 회수율을 검토하였다. 26가지 제품에 대한 회수율 검토결과 Orange II는 96.46-121.26%, Rhodamine B는 70.86-106.53%, Para red는 97.00-116.86%, Sudan I은 92.93-112.44%, Sudan II는 96.63-115.10%, Sudan III는 92.21-114.73%, Sudan IV는 93.22-122.91%의 회수율을 나타냈다. 본 분석법 검량선의 상관계수는 0.999 이상을 보였으며 정밀성의 RSD는 0.8-1.39%로서 모두 2 이하의 값을 보였으며, 정확성은 90% 이상을 나타냈다. 이때, 검출한계 및 정량한계는 각각 0.1 mg/L, 0.3 mg/L로 결정하였다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 학술대회지
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• pp.862-866
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• 2004

Non-intrusive methods based on active remote IR illumination for eye tracking is important for many applications of vision-based man-machine interaction. One problem that has plagued those methods is their sensitivity to lighting condition change. This tends to significantly limit their scope of application. In this paper, we present a new real-time eye detection and tracking methodology that works under variable and realistic lighting conditions. Based on combining the bright-pupil effect resulted from IR light and the conventional appearance-based object recognition technique, our method can robustly track eyes when the pupils are not very bright due to significant external illumination interferences. The appearance model is incorporated in both eyes detection and tracking via the use of support vector machine and the mean shift tracking. Additional improvement is achieved from modifying the image acquisition apparatus including the illuminator and the camera.