Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Norovirus Targeted Bioreceptor Screening Method based on Lateral Flow Immunoassay (LFIA)

노로바이러스 검출을 위한 측면유동면역분석법 기반의 바이오리셉터 선별기법 개발

  • Huisoo, Jang (Department of Biological Sciences and Bioengineering, Inha University) ;
  • Hyeonji, Cho (Department of Biological Engineering, Inha University) ;
  • Tae-Joon, Jeon (Department of Biological Engineering, Inha University) ;
  • Sun Min, Kim (Department of Mechanical Engineering, Inha University)
  • 장희수 ;
  • 조현지 ;
  • 전태준 ;
  • 김선민
  • Received : 2022.11.09
  • Accepted : 2022.11.22
  • Published : 2022.11.30
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Abstract

Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 1012 copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(세부과제명: 농축산물 생산현장의 안전관리 기술개발, 세부과제번호: PJ014238042022)과 환경부의 재원으로 한국환경산업기술원(사업명: 생활화학제품 안전관리기술개발사업, 과제번호: RE202201777)의 지원에 의해 이루어진 것임

References

  1. S. Sharma, H. Byrne, and R. J. O'Kennedy, 2016, "Antibodies and antibody-derived analytical biosensors," Essays Biochem, vol. 60, pp. 9-18. https://doi.org/10.1042/EBC20150002
  2. N. Bhalla, P. Jolly, N. Formisano, and P. Estrela, 2016, "Introduction to biosensors," Essays Biochem, vol. 60(1), pp. 1-8. https://doi.org/10.1042/EBC20150001
  3. S. A. Lim and M. U. Ahmed, 2019, "CHAPTER 1: Introduction to Immunosensors," in RSC Detection Science, vol. 2019-January, no. 14, Royal Society of Chemistry, pp. 1-20.
  4. F. B. Arslan, K. Ozturk Atar, and S. Calis, 2021, "Antibody-mediated drug delivery," International Journal of Pharmaceutics, vol. 596.
  5. S. Aydin, 2015, "A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA," Peptides (N.Y.), vol. 72, pp. 4-15. https://doi.org/10.1016/j.peptides.2015.04.012
  6. T. Mahmood and P. C. Yang, 2012, "Western blot: Technique, theory, and trouble shooting," N Am J Med Sci, vol. 4(9), pp. 429-434. https://doi.org/10.4103/1947-2714.100998
  7. C. Parolo et al., 2020, "Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays," Nature Protocols, vol. 15(12), pp. 3788-3816. https://doi.org/10.1038/s41596-020-0357-x
  8. A. Somborac Bacura, M. Dorotic, L. Grosic, M. Dzimbeg, and S. Dodig, 2021, "Current status of the lateral flow immunoassay for the detection of sars-cov-2 in nasopharyngeal swabs," Biochem Med (Zagreb), vol. 31(2)
  9. Merck Millipore, 2022, "Rapid Lateral Flow Test Strips Considerations for Product Development." Accessed: Oct. 26, 2022. [Online]. Available: https://www.merckmillipore.com/KR/en
  10. J. A. Wippold, H. Wang, J. Tingling, J. L. Leibowitz, P. de Figueiredo, and A. Han, 2020, "PRESCIENT: platform for the rapid evaluation of antibody success using integrated microfluidics enabled technology," Lab Chip, vol. 20(9), pp. 1628-1638. https://doi.org/10.1039/c9lc01165j
  11. C. P. Campillay-Veliz et al., 2020, "Human Norovirus Proteins: Implications in the Replicative Cycle, Pathogenesis, and the Host Immune Response," Frontiers in Immunology, vol. 11.
  12. K. Rupprom, P. Chavalitshewinkoon-Petmitr, P. Diraphat, and L. Kittigul, "Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II," Virol Sin, vol. 32(2), pp. 139-146
  13. H. S. Kim, J. Hyun, J. S. Kim, W. Song, H. J. Kang, and K. M. Lee, 2012, "Evaluation of the SD Bioline Norovirus rapid immunochromatography test using fecal specimens from Korean gastroenteritis patients," J Virol Methods, vol. 186(1-2), pp. 94-98. https://doi.org/10.1016/j.jviromet.2012.08.014
  14. T. You, W. Jeong, H. Lee, Y. S. Huh, S. M. Kim, and T. J. Jeon, 2021, "A simple strategy for signal enhancement in lateral flow assays using superabsorbent polymers," Microchimica Acta, vol. 188(11).
  15. D. S. Kim et al., 2016, "Development of lateral flow assay based on size-controlled gold nanoparticles for detection of hepatitis B surface antigen," Sensors (Switzerland), vol. 16(12).
  16. X. I. Jiang, M. Wang, D. Y. Graham, M. K. Estes', B. Plaza, and G. Section, 1992, "Expression, Self-Assembly, and Antigenicity of the Norwalk Virus Capsid Protein,"
  17. D. Gasperino, T. Baughman, H. v Hsieh, D. Bell, and B. H. Weigl, 2018, "Improving Lateral Flow Assay Performance Using Computational Modeling,"