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http://dx.doi.org/10.4333/KPS.2004.34.4.327

Validation of a Simple HPLC Method for Determination of Ciprofloxacin Hydrochloride in Human Plasma  

Ha, Yong-Hwa (College of Pharmacy, Kyung Hee University)
Cho, Sung-Hee (College of Pharmacy, Kyung Hee University)
Chun, Sung-Kuk (College of Pharmacy, Kyung Hee University)
Seo, Seong-Hoon (College of Pharmacy, Kyung Hee University)
Rew, Jae-Hwan (Medical Center, Kyung Hee University)
Choi, Young-Wook (College of Pharmacy, Chung Ang University)
Lee, Kyung-Tae (College of Pharmacy, Kyung Hee University)
Publication Information
Journal of Pharmaceutical Investigation / v.34, no.4, 2004 , pp. 327-331 More about this Journal
Abstract
A simple HPLC method with ultraviolet detection of ciprofloxacin in human plasma was developed and validated. After protein precipitation with trichloroacetic acid, chromatographic separation of ciprofloxacin in plasma was achieved at $50^{\circ}C$ with a $C_{18}$ column and methanol-phosphate mixture (pH 2.5), as mobile phase. Quantitative determination was performed by ultraviolet detection at 278 nm. The method was specific and validated with a limit of quantification of 100 ng/ml. The intra- and inter-day coefficients of variation were between 1.67% and 10.55% and accuracy between 92.01 % and 106.09%. The method has been successfully applied in a bioavailability study of 250 mg ciprofloxacin hydrochloride tablet.
Keywords
Ciprofloxacin hydrochlorode; Validation; Bioavailability; HPLC;
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