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http://dx.doi.org/10.3839/jabc.2015.060

Development of Duplex PCR Method for Simultaneous Detection of Rabbit (Oryctolagus cuniculus) and Cat (Felis catus) Meats  

Hong, Yeun (Department of Food Science & Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Kim, Mi-Ju (Department of Food Science & Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Yang, Seung-Min (Department of Food Science & Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Yoo, In-Suk (Department of Food Science & Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Kim, Hae-Yeong (Department of Food Science & Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.4, 2015 , pp. 383-387 More about this Journal
Abstract
A duplex polymerase chain reaction (PCR) detection method was developed to authenticate the use of cat and rabbit in food and to prevent unlawful distribution of illegally butchered meat in both domestic and imported food market. Species-specific primers were designed targeting mitochondrial cytochrome b gene. The sizes of PCR products were 191 bp for cat and 101 bp for rabbit, which were relatively small for better application of the detection method on processed foods. Specificities of primers were verified using 21 animal species including cat and rabbit. Limit of detection was examined by serial dilution of the sample DNA and confirmed as 0.005 ng for rabbit and 0.0005 ng for cat using Bioanalyzer. The developed duplex PCR method showed specificity and sensitivity in the identification of two target species.
Keywords
cat; duplex polymerase chain reaction; food authentication; multiplex polymerase chain reaction; rabbit;
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Times Cited By KSCI : 5  (Citation Analysis)
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