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Investigation on Korean Local Maize Lines V. Variabilities of Plant Characters of Multi-eared and Tillered Lines(MET) (재래종 옥수수 수집종에 대한 특성조사 제5보 다수다벽 재래종 옥수수계통의 특성변이)

  • Choe, B.H.;Park, J.S.;Kim, Y.R.;Park, K.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.1
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    • pp.56-68
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    • 1981
  • A maize line was selected in 1979 among 1000 Korean local maize lines collected in 1977. The selected maize line was characterized by having three to four tillers and eight to 10 ears on each individual plant. The line was assumed to have a great potential as a silage crop. The investigation was conducted as one of the serial studies on the Korean maize collected lines to provide basic information on the genetic variabilities of the multi-eared and tillered (MET) line and on other agronomic characters, prior to use the line as material for future breeding works for silage crop. The MET line and Suwon #19, single cross hybrid, as check variety were planted on May 1, 15 and 30, in three different levels of plant populations. The results obtained were summarized as follows: 1. The genetic variabilities of multi-ear and tillering habits were greater than environmental variabilities. 2. Total dry leaf weight of individual plant of MET line was also significantly higher than that of Suwon #19. 3. The mean number of tillers and ears bearing on the individual plant of MET line varied greatly with plant densities. The number of tillers and ears was on the average 2.9 and 7.0, respectively, when planted in 60cm. by 60cm. 4. The total dry matter and dried stem weight of the individual plant on MET line were comparable to those of Suwon #19. 5. The kernel weight from the individual plant of MET line was 5 to 40% less than that of Suwon #19, depending upon the plant densities. 6. The Kernel to stover ratio was higher for Suwon #19 than for the MET line. (41% to 35%). 7. The MET line had shown first tiller two weeks after planted on May 1. The second and third tillers appeared three to five days after the appearance of the first tiller. 8. The MET line was very specific in tillering habits. All the tillers were borne on the first few nodes of main stem below the soil surface. 9. The tillering habits of MET line were vigorous in the early part of the growing season, but less vigorous in the later part of the growing season. The number of efficient tillers bearing useable ears, was around two to three, when planted in 60cm. by 60cm. 10. The difference of plant height between main stem and first few tillers was around 10cm. 11. The ear size of MET line was around one-third of the major corn belt hybrids. The shape of ear of MET line was conical, with different diameter. 12. The kernel of the MET line was flinty with small soft starch patches on the endosperm part. 13. The 100 kernel weight was around 15gr., which is about one half of the major high yielding hybrids. 14. The ear height of MET line was comparatively higher than that of Suwon #19. 15. Significantly high and positive phenotypic correlation coefficients were obtained among major plant characters. 16. The growth rate of MET line was slower than that of Suwon #19. 17. MET line and Suwon #19 were both heavily infected with black streaked mosaic virus.

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Genome Organization and Transcription Response to Harvest of Two Metallothionein-Like Genes in Agaricus bisporus Fruiting Bodies

  • Eastwood, Daniel C.;Bains, Navdeep K.;Henderson, Janey;Burton, Kerry S.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.5
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    • pp.455-463
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    • 2011
  • Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar mt-like genes (met1 and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested.

A Comparison of Offshore Met-mast and Lidar Wind Measurements at Various Heights (해상기상탑과 윈드 라이다의 높이별 풍황관측자료 비교)

  • Kim, Ji Young;Kim, Min Suek
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.29 no.1
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    • pp.12-19
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    • 2017
  • There is a need to substitute offshore met-mast with remote sensing equipment such as wind lidar since the initial installation and O&M costs for offshore met-mast are quite high. In this study, applicability of wind lidar is verified by intercomparison test of wind speed and direction data from offshore met-mast and wind lidar for simultaneous operational period. Results at various heights show no statistical difference in trend and size and data from wind lidar is found to be more accurate and have less error than data from offshore met-mast where error from structural shading effect is significant.

The Relationship between Early-onset Androgenetic Alopecia and Metabolic Syndrome: Systematic Review and Meta-Analysis (조발성 탈모증과 대사증후군과의 관계: 체계적 문헌고찰 및 메타분석)

  • Jang, Jin-Young;Yoon, Young-Joon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.30 no.3
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    • pp.166-181
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    • 2017
  • Objectives : The study was done to verify the relation between early-onset androgenetic alopecia(AGA) and metabolic syndrome(MetS). Methods : Data were collected through electronic database including KoreaMed, National Assembly Library, KMBASE, NDSL, KCI, KERIS, Google Scholar, Pubmed, Cochrane CENTRAL and EBSCO MEDLINE. A total of 13 case-control studies related to the MetS of early-onset alopecia patients were used for the systematic review and meta-analysis. Risk of bias of included studies were assessed by RoBANS tool. RevMan5.3, CMA3 were used for the meta-analysis. Results : In 13 evaluated articles, most frequent bias was the participant selection bias that was found in 10 articles. Significant association between early-onset AGA and MetS was found in 10(76.9%) out of 13 articles in the systematic review. In meta-analysis, early-onset male AGA was associated with increased risk of metabolic syndrome(OR: 3.73, 95% CI:2.49 -5.61). Conclusions : AGA, particularly early -onset male AGA, is significantly associated with MetS. Therefore all patients with early onset male AGA should be suggested to take preventive treatment to reduce the risk of MetS and various problems associated with it.

Effects of Medical Exercise Therapy on Walking Ability and Ankle Muscles Activation after Chronic Stroke (Medical Exercise Therapy가 만성 뇌졸중 환자의 보행능력 및 족관절 근활성도에 미치는 영향)

  • Cho, Yuong-Hwan;Park, Jong-Hang;Kim, Kyung-Yoon;Nam, Ki-Won
    • Journal of the Korean Society of Physical Medicine
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    • v.6 no.3
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    • pp.311-321
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    • 2011
  • Purpose : The purpose of this study was to determine effect of Medical Exercise therapy on walking ability and ankle muscles activation after stroke. Method : Participants were randomly divided into either MET training group(n=12) and control group(n=12). All of participants had been receving a traditional rehabilitation program, 5 days a week. MET training group have additionally undergone for six weeks, 3 days a week, the MET program. But control group was not received any additional program except the traditional rehabilitation program. The 10 mWT, F8WT and 2 MWT to measure walking ability were carried out twice before and after training. Muscular activity of the ankle was estimated by analyzing the RMS of action potential for EMG in terms of tibialis anterior muscle(TA), soleus(SO), gastrocnemius medial head(GM), gastrocnemius lateral head(GL). Rresults : At the walking ability, MET training group demonstrated a significant improvememt in the score of the 10 mWT(p<0.001), 2 MWT(p<0.05), but F8WT was not significant improvement(p>0.05). At the ankle muscle activation, MET training group demonstrated a significant improvememt in the RMS of the TA(p<0.01) and SO(p<0.05) but GM(p>0.05) and GL(p>0.05) were not significant improvement. Conclusion : From these results of this study, MET training for 6 weeks has an effect on improvement of walking ability and ankle muscles activation after stroke.

Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize

  • Jang Cheol Seong;Lee Hee Bong;Seo Yong Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.358-362
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    • 2004
  • In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

Towards Methionine Overproduction in Corynebacterium glutamicum - Methanethiol and Dimethyldisulfide as Reduced Sulfur Sources

  • Bolten, Christoph J.;Schroder, Hartwig;Dickschat, Jeroen;Wittmann, Christoph
    • Journal of Microbiology and Biotechnology
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    • v.20 no.8
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    • pp.1196-1203
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    • 2010
  • In the present work, methanethiol and dimethyldisulfide were investigated as sulfur sources for methionine synthesis in Corynebacterium glutamicum. In silico pathway analysis predicted a high methionine yield for these reduced compounds, provided that they could be utilized. Wild-type cells were able to grow on both methanethiol and dimethyldisulfide as sole sulfur sources. Isotope labeling studies with mutant strains, exhibiting targeted modification of methionine biosynthesis, gave detailed insight into the underlying pathways involved in the assimilation of methanethiol and dimethyldisulfide. Both sulfur compounds are incorporated as an entire molecule, adding the terminal S-$CH_3$ group to O-acetylhomoserine. In this reaction, methionine is directly formed. MetY (O-acetylhomoserine sulfhydrylase) was identified as the enzyme catalyzing the reaction. The deletion of metY resulted in methionine auxotrophic strains grown on methanethiol or dimethyldisulfide as sole sulfur sources. Plasmid-based overexpression of metY in the ${\Delta}$metY background restored the capacity to grow on methanethiol or dimethyldisulfide as sole sulfur sources. In vitro studies with the C. glutamicum wild type revealed a relatively low activity of MetY for methanethiol (63 mU/mg) and dimethyldisulfide (61 mU/mg). Overexpression of metY increased the in vitro activity to 1,780 mU/mg and was beneficial for methionine production, since the intracellular methionine pool was increased 2-fold in the engineered strain. This positive effect was limited by a depletion of the metY substrate O-acetylhomoserine, suggesting a need for further metabolic engineering targets towards competitive production strains.

Influence of Occupational Type and Lifestyle Risk Factors on Prevalence of Metabolic Syndrome among Male Workers: A Retrospective Cohort Study (남성근로자의 대사증후군 유병에 영향을 미치는 직업군 및 생활습관 위험인자: 후향적 코호트 조사연구)

  • Kang, So Hui;Hwang, Seon Young
    • Korean Journal of Adult Nursing
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    • v.28 no.2
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    • pp.180-190
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    • 2016
  • Purpose: This study examined the influence of occupational type and lifestyle habits on the prevalence of metabolic syndrome (MetS) among Korean male workers. Methods: Through secondary analysis of their four-year health examination data, 3,892 subjects were divided into four subgroups according to the presence of MetS now and four years ago. Results: Nineteen percent (n=739) suffered from MetS and these 739 subjects were classified into following occupations: 7.1% were office workers, 17.6% were non-office workers, and 42.2% were drivers. Multiple logistic regression analyses showed that when the data adjusted for age, the predicting factors on the prevalence of MetS were heavy drinking (OR 1.34, 95% CI 1.09~1.64) and the occupation of non-office workers (OR 2.99, 95% CI 2.13~4.18) and drivers (OR 7.97, 95% CI 4.89~10.83) among workers without MetS four years ago. Among workers already with a history of MetS, the predicting factors were less exercise (OR 1.55, 95% CI 1.02~2.35) and drivers (OR 2.21, 95% CI 1.03~2.94). Conclusion: Heavy drinking and less exercise and drivers were reported as influencing factors on the prevalence of MetS by this sample. The findings suggest that employers need to provide their employees with screening and management program for those at risk of MetS.

Association of Osteoarthritis with prevalence of Metabolic syndrome: based on 2009-2010 Korean National Health & Nutrition Survey (골관절염과 대사증후군과의 상관관계: 2009-2010년 국민건강영양조사 이용)

  • Oh, Ji-Eun;Lim, Se-Hyun
    • Journal of Digital Convergence
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    • v.14 no.7
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    • pp.269-277
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    • 2016
  • This study was attempted to investigate the possible association of osteoarthritis with the metabolic syndrome (MetS) and its components in Korean adults based on the Korean National Health and Nutrition Examination Survey (KNHANES). The prevalence of MetS was significantly increased in OA group in women. To investigate the association between the components for MetS and osteoarthritis, we analyzed the mean values of 5 components of MetS, waist circumference (WC), triglyceride (TG), HDL-cholesterol, systolic blood pressure, and fasting plasma glucose in normal and OA group. In women, WC, TG, HDL, and systolic blood pressure were associated with the development of OA. Moreover, accumulation of components of MetS was significantly related to the prevalence of OA in Korean men and women. These data suggest that MetS might be a novel risk factor for OA and MetS should be taken into account in the prevention and treatment of OA for Korean adults as well.

Degradation and Stabilization of Methionine Enkephalin and $[D-Ala^2]-methionine$ Enkephalinamide in the Corneal Extracts of Rabbits (토끼의 각막 추출액 중 메치오닌엔케팔린 및 [D-알라$^2$-메치오닌엔케팔린아미드의 분해 및 안정화)

  • Lee, Chi-Ho;Lee, Kyoung-Jin;Chun, In-Koo;Sung, Young-Gi;Shin, Young-Hee
    • Journal of Pharmaceutical Investigation
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    • v.24 no.1
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    • pp.1-9
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    • 1994
  • In order to study systemic peptide delivery through the ocular route, the stabilities of methionine enkephalin (Met-Enk) and $[D-ala^2]-methionine$ enkephalinamide (YAGFM) in the corneal extracts of rabbits were investigated using reversed phase HPLC. Met-Enk was found to be hydrolyzed most rapidly in the corneal epithelium, but YAGFM was relatively stable. Aminopeptidases appeared to contribute over 60% to the degradation of Met-Enk and the degradation rate of Met-Enk followed the first order kinetics. The half-lives of Met-Enk in the extracts of the corneal epithelium and endothelium were 36 and 673 min, respectively. From the effects of enzyme inhibitors, it was found that the application of the mixture of amastatin, thimerosal and EDTA was very useful for the inhibition of peptide degradation.

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