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http://dx.doi.org/10.4014/jmb.1007.07031

Genome Organization and Transcription Response to Harvest of Two Metallothionein-Like Genes in Agaricus bisporus Fruiting Bodies  

Eastwood, Daniel C. (Department of Bioscience, College of Science, University of Swansea)
Bains, Navdeep K. (Warwick HRI, University of Warwick)
Henderson, Janey (School of Natural and Environmental Sciences, Coventry University)
Burton, Kerry S. (Warwick HRI, University of Warwick)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.5, 2011 , pp. 455-463 More about this Journal
Abstract
Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar mt-like genes (met1 and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested.
Keywords
Metallothionein; Agaricus bisporus; bidirectional promoter;
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