• Title/Summary/Keyword: mesocotyl

Search Result 30, Processing Time 0.027 seconds

Changes of Cytosolic $Ca^{2+}$ by IAA and Zeatin in Protoplasts Isolated from Maize Mesocotyl (옥수수 중배축으로부터 분리한 원형질체에서 IAA와 Aeatin에 의한 세포질 $Ca^{2+}$ 노도의 변화)

  • 송재진
    • Journal of Plant Biology
    • /
    • v.34 no.3
    • /
    • pp.239-244
    • /
    • 1991
  • Ca2+ is implicated as a second messenger in coupling various stimuli such as hormone, gravity and light. The determine whether or not plant hormones mobilize calcium with different action, we investigated the cytosolic Ca2+ changes by IAA and zeatin in the protoplasts isolated from elongating mesocotyl of maize. IAA increased the influx of Ca2+ due to the calcium channel opening, which was confirmed by using verapamil, calcium channel blocker. On the other hand, zeatin increased the cytosolic Ca2+ by promoting the efflux of Ca2+ derived from cellular organelles. These results suggest that different calcium flux induced by IAA and zeatin plays a role in appropriate response resulting in increase of cell elongation or repression cell elongatoin.

  • PDF

Seedling Emergence and Initial Growth between Water and Dry Seeding in Forage Millet (사료용 피의 건답과 무논 파종 시 입모 및 초기생육특성)

  • Ahn, Seok-Hyeon;Chung, Nam-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.59 no.1
    • /
    • pp.83-88
    • /
    • 2014
  • This research was carried out to determine the characteristics of germination in relation to temperature, and seedling emergence and growth affected by soil moisture in forage millet. The seeds of forage millet could germinate at more than $15^{\circ}C$ at the rate of 0, 90.5, 97.3 and 96.8% respectively 10 days after seeding at 10, 15, 20 and $25^{\circ}C$. The result showed that the temperature needs to be $15^{\circ}C$ or more for the seeds to germinate. The effects of seeding depth on the growth characteristics and emergence of millet seed were investigated in waters and direct seeding methods by thoroughly mixing the seed and soil. In water treatment, the emergence rates were 3.7% at $15^{\circ}C$, 7.6% at $20^{\circ}C$, 6.3% at $25^{\circ}C$, while direct seeding treatment showed the emergence rates of 65.6, 75 and 71% at 15, 20 and $25^{\circ}C$, respectively. At 15, 20, and $25^{\circ}C$, seeds in water seeding could germinate at 0.5, 1.8, 1.5 cm of soil depth, while in direct seeding, they could germinate at the depth of 9.5, 10.0, 9.9 cm. When the initial growth characteristics of the seeds were investigated, there was positive correlation between seeding depth and mesocotyl length in both water and direct seed methods. Growth and development in terms of leaf age, root length, coleoptiles and mesocotyl length, and plant height were found maximum at 0.5 cm soil depth for water seeding, and 3-5 cm soil depth for direct seeding. Results showed that there were close relationship among temperature, soil depth and moisture acquiring methods, and the combination of these factors greatly affected the initial growth characteristics and development of millet seeds. It can be concluded that, to get good seedling stand and germination of millet seed, millet should grown in field condition at the depth of 0.5 cm or less for water seeding, and 3-5 cm for direct seeding method.

Sprouting and Emergence Properties of Eleocharis kuroguwai Ohwi (올방개 괴경(塊莖)의 맹아(萌芽) 및 출현(出現) 특성(特性))

  • Lee, H.K.;Lee, I.Y.;Ryu, G.H.;Lee, J.O.;Lee, E.J.
    • Korean Journal of Weed Science
    • /
    • v.14 no.4
    • /
    • pp.233-238
    • /
    • 1994
  • The experiment on sprouting and emergence properties of Eleocharis kuroguwai was conducted at laboratory and greenhouse conditions in 1993. Most of tubers had the apical dominance of buds at sprouting and had the competitive relationship for mesocotyl elongation. When pyrazosulfuron was applied on soil surface, all of apical buds were killed, but the first and second lateral buds survived to 50% and 90%, respectively, and the third lateral buds were almost at presprouted state. The tubers at 10-15cm deep soil emerged 1 week late and 35% low in emergence rate compared with the tubers at 2-5cm deep. As the plants emerged from the deep soil, the mesocotyl and the roots were distributed at deep soil more or less. Among the tubers buried at 10cm and 15cm deep soil, 25% and 30% respectively were not emergerd due to the suspension of mesocotyl elongation. Although the roots and shoots were removed from the tubers at the stage of 20cm high, all of mother tubers emerged again even 2 weeks late.

  • PDF

Seedling Emergence and Mesocotyl Elongation as affected by Temperature and Seeding Depth in Direct-seeded Rice on Dry Soil (벼 건답직파재배에서 온도 및 파종심도가 종자의 출아와 중배축 신장에 미치는 영향)

  • Lee, Chul-Won;Yun, Yong-Dae;Oh, Yun-Jin;Cho, Sang-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.37 no.6
    • /
    • pp.534-540
    • /
    • 1992
  • Climatic condition and seeding depth affect the seedling stand and early growth in the direct-seeded rice cultivation on dry soil. This experiment was conducted to elucidate the effects of the day /night temperatures and the seeding depths on the seedling emergence and mesocotyl elongation of rice seed. Three combinations of the day/night temperatures(25/2$0^{\circ}C$ 20/15$^{\circ}C$ and 20/1$0^{\circ}C$) were employed with seeding depths 1, 3, 5 and 7cm at the Phytotron of the Crop Experiment Station in 1991. It appeared that seedling emergence ratio increased and days to seedling emergence decreased in the high temperature (25/2$0^{\circ}C$) and the deep seeding depth (5 and 7cm) condition. The seedling emergence ratio did not. show the, difference up . to the seeding depths of 3cm and below, but the ratio decreased from the seeding depths of 5cm and above. Plant height and leaf number were almost the same up to the depths of 3cm at 30 days after seeding, but those of the seeding depths of 5cm and 7cm were remarkably reduced in all temperature combinations. Mesocotyl and lower internode elongation were seen in the high temperature(25/2$0^{\circ}C$) with the seeding depths of 5cm and 7cm. In the seeding depth of 6cm, of the tested varieties, Tamjinbyeo and Odaebyeo showed the highest emergence ratio. Generally, leaves of all tested varieties appeared approximately in the soil depth of 3cm, so the reasonable seeding depth will be around 3cm in the direct-seeded cultivation on dry soil.

  • PDF

Reconstitution of Membrane Proteins from Corn Seedlings with Phosphatidylcholine (옥수수 유식물 조직에서 분리한 막 단백질과 Phosphatidylcholine의 재조합)

  • 오승은
    • Journal of Plant Biology
    • /
    • v.33 no.4
    • /
    • pp.321-323
    • /
    • 1990
  • Membrane proteins isolated from the coleoptile and mesocotyl tissues of corn seedlings were solubilized with Triton X100 and reconstituted with phosphatidylcholine at 2$0^{\circ}C$. The proteoliposomes were incubated and proton uptake into the vesicles was measured with a spectrophotometer. Addition of ATP to the reaction mixture was found to result in an active accumulation of proton into the vesicles. These results indicate that the preparation contains tightly bound phosphatidylcholine vesicles with reconstituted H+ -ATPase from the plant cell membranes.

  • PDF

Seedling Emergence of Dry -seeded Rice under Different Sowing Depths and Irrigation Regimes (건답직파에서 파종심도와 관개조건에 따른 벼 품종들의 출아특성)

  • 이변우;명을재
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.40 no.1
    • /
    • pp.59-68
    • /
    • 1995
  • Investigated were the relationships between plumule elongation characteristics and seedling emergence of 46 varieties including native, improved and red rice varieties of Korea, and varieties from U.S.A., Italy, India, Japan under 1, 3, and 5cm deep sowing with irrigated and non-irrigated condition. Experiments were carried out in paddy field of sandy loam. There was heavy shower of 19.2mm on the next day of seeding and thereafter, clear and dry weather continued during the experiment period. Soil temperature averaged over 30 days after seeding was $16.4^{\circ}C$ at 3cm depth. Soil hardness increased linearly up to 2.5kg /$cm^2$ on the 14th day after seeding, on which date irrigated plot was irrigated through furrow, and up to 4kg / $cm^2$ on the 28th day in non-irrigated plot. Soil hardness dropped near to 0kg /$cm^2$ after irrigation and developed up to 2.5kg /$cm^2$ again by 28 days after seeding. Seedling emergence was higher in irrigated plots than non-irrigated plots at all seeding depths. Korean improved varieties were substantially lower in seedling emergence under non-irrigated condition of 1 cm deep sowing than those under irrigated condition. This poor seedling emergence resulted mainly from delayed emergence by exposing them to greater soil strength. Percent seedling emergence under irrigated and non-irrigated condition showed signifi-cant correlations at 3 and 5 cm deep sowing. Korean improved varieties belonged to the group of poor seedling emergence, and I taliconaverneco, Chinsura Boro and Weld Pally to best group under both irrigation conditions at 3 and 5cm deep sowing. Seedling emergence showed highly signifi-cant positive correlation with the plumule length of mesocotyl + 1st internode + incomplete leaf and of mesocotyl+coleoptile. Among the characters constituting plumule length, incomplete leaf length showed greatest positive correlation followed by coleoptile and mesocotyl under irrigated condition at 3 and 5 cm deep sowing, and highest correlation with mesocotyllength followed by first internode and incomplete leaf under non-irrigated condition. Days to 50% seedling emergence at 1 cm deep sowing with irrigation showed great varietal variation of 10 to 30 days, and showed high significant negative correlations with percent seedling emergence under both irrigation conditions except for 1 cm deep sowing with irrigation, Days to seedling emergence revealed sig-nificant negative correlations with plumule characters except 2nd internode, showing highest cor-relation with incomplete leaf length.

  • PDF