Changes of Cytosolic $Ca^{2+}$ by IAA and Zeatin in Protoplasts Isolated from Maize Mesocotyl

옥수수 중배축으로부터 분리한 원형질체에서 IAA와 Aeatin에 의한 세포질 $Ca^{2+}$ 노도의 변화

Ca2+ is implicated as a second messenger in coupling various stimuli such as hormone, gravity and light. The determine whether or not plant hormones mobilize calcium with different action, we investigated the cytosolic Ca2+ changes by IAA and zeatin in the protoplasts isolated from elongating mesocotyl of maize. IAA increased the influx of Ca2+ due to the calcium channel opening, which was confirmed by using verapamil, calcium channel blocker. On the other hand, zeatin increased the cytosolic Ca2+ by promoting the efflux of Ca2+ derived from cellular organelles. These results suggest that different calcium flux induced by IAA and zeatin plays a role in appropriate response resulting in increase of cell elongation or repression cell elongatoin.