• Title/Summary/Keyword: medium extension

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In Vitro Micropropagation of Polygonatum odoratum cv. Gungangbeaksea (둥굴레 '건강백세'의 기내 대량번식)

  • Kim, Man Bae;Park, Chun Geun;Yi, Sookyi;Han, Bong Hee
    • Korean Journal of Plant Resources
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    • v.27 no.5
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    • pp.540-545
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    • 2014
  • The Polygonatum odoratum cv. Gungangbeaksea, bred in Gyeongsangnam-Do Agricutural Research & Extension Service, was cultured in vitro for micropropagate rapidly through the culture of rhizome explants ($5{\times}5mm$). The $7{\times}7mm$ explants of adventitious multi-bud clusters (AMC), obtained through the culture of rhizome explants (MS + 3.0 mg/L BA) were cultured on MS media with BA and TDZ. The shoot multiplication was favorable on the MS medium containing 3.0 mg/L TDZ with 2.8 in shoot number. But the formation of AMC was low in all media tested. The explants of AMC were cultured on MS media containing 1.0~5.0 mg/L TDZ and NAA to multiplicate AMC more. The formation of AMC was a little more stimulated on combined MS media of TDZ and NAA, than that with TDZ alone. The multiplication of shoots and AMC was favorable on MS media with 3.0 mg/L TDZ and 5.0 mg/L NAA, and 5.0 mg/L TDZ and 3.0 mg/L NAA. As the concentration of MS salts increased, the formation of AMC was decreased. But the formation of AMC was more stimulated, as the concentration of sucrose increased to 7%. Therefore, the multiplication of shoots and AMC was suitable on media containing 3.0~5.0 mg/L TDZ and NAA, and 7% of sucrose. The explants of AMC were rooted on media with 3.0 mg/L IBA, or 2.0 mg/L NAA with more than 80% in rooting ratio. The plantlets were treated at $5^{\circ}C$ for 8 weeks, and cultured ex vitro for 8 weeks. The survival ratio of plantlets were 100% in vermiculite, and the mixed soil with perlite 1 volumn and vermiculite 1 volumn.

A Systematic Approach to Port related Problems An Analysis on the Actual Condition of physical Distribution System of Pusan port (항만관련문제의 시스템적 고찰 부산항 물류시스템의 실태분석)

  • Lee Cheol-Yeong;Moon Seong-Hyeok
    • Journal of Korean Port Research
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    • v.2 no.1
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    • pp.7-28
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    • 1988
  • From the viewpoint of physical distribution, the port transport process can be regarded as a system which consists of various subsystems such as navigational aids, quay handling, transfer, storage, information If management, and co-ordination with inland transport. The handling productivity of this system is determined by the production level of the least productive subsystem. So, a productivity analysis on the flow of cargoes through each subsystem should be made in order to achieve efficient port operation. The purpose of this paper is to analyze the productivity of each subsystem in Pusan port, and to bring forward problems and finally to draw up plans for their betterment. Analyzed results on the productivity of each subsystem are as follows, i) It is known that the number of tugs with low HP should be increased by a few, the number of tugs with medium HP is appropriate, and the number of tugs with high HP is in excess of that necessary. ii ) In the case of container cargoes, it is found that the transfer and storage systems in BCTOC have the lowest handling capability, with a rate of $115\%$, leading to bottle-necks in the port transport system, while the handling rate of the storage and quay handling systems in general piers is in excess of the inherent capability. iii) In the case of the principal seaborne cargoes passing through general piers, there is found to be a remarkable bottle-neck in the storage system. In the light of these findings, both the extension of storage capability and the extension of handling productivity are urgently required to meet the needs of port users. Therefore, iv) As a short-term plan, it is proposed that many measures such as the reduction of free time, the efficient application of ODCY, etc must be brought in and v) In the long-trun, even though the handling capability will accommodate an additional 960,000 TEU in 1991, the scheduled completion date of the third development plan of Pusan port, insufficiency of handling facilities in the container terminal is still expected and concrete countermeasures will ultimately have to be taken for the port's harmonious operation. In particular, the problem of co-ordination with inland transport and urban traffic should be seriously examined together in the establishment of the Pusan port development. As a method of solving this, vi) It is suggested that Pusan port (North port) should be converted into an exclusive container ternimal and overall distribution systems to the other ports for treating general cargoes must be established. vii) And finally, it is also proposed that the arrival time (cut-off time) of influx cargoes for exports such as general merchandise and steel product should be limited, with a view to securing cargoes suitable for the operational capability of BCTOC.

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A Study on the Feasibility of the Improvement of Traffic Congestion : Focusing on Small and Medium-Sized Cities in Chuncheong Province (중소도시 교통혼잡도로 적용범위에 관한 연구 : 충청권 중심으로)

  • Kwon, Hyun Joong;Oh, Ju Taek
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.18 no.3
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    • pp.34-45
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    • 2019
  • Based on the analysis of congestion conditions in small cities with a population of less than 500,000 in Chungcheong Province, this study conducted a feasibility study on the scope of congestion improvement projects. Further, traffic congestion management standards were further established by analyzing LOS as well as the speed of traffic presented by congestion criteria in the existing Project. According to the analysis according to the congestion management standard LOS suggested in this study, Asan city has the most major number of highways where LOS E occurs for more than 3 hours, followed by Sejong City and Gongju City. In addition, when comparing the ratio of the busy main roads according to the frequency of LOS E by city size, it was analyzed that the ratio and extension of the busy highways relative to the overall extension of the large Chungcheong area are similar to the ratio in small cities in the Chungcheong region. Therefore, traffic congestion occurred in small cities and the feasibility of the improvement project was revealed.

A New Paradigm in the Distribution of Sport Contents: Sports as a New Media (스포츠 콘텐츠 유통의 새로운 패러다임: 스포츠의 뉴미디어화)

  • Park, Seong-Hee;Han, Seung-Jin;Seo, Won-Jae
    • Journal of Distribution Science
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    • v.15 no.10
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    • pp.93-103
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    • 2017
  • Purpose - Sports and the media have been developed together through a close relationship. During the past decade, the media landscape and the coverage of sports events have been changed. Sports fans can use the sports content at the time they want, on the platform they prefer. Furthermore, thanks to the advanced information technology, sports fans are likely to be more engaged in sport in communication technology-friendly stadium. However, the literature on the relationship between sports and the media has heavily focused on the differences of media types, clear distinction between media suppliers and consumers, and the limited media extension. Given the limitation of prior research, therefore, it has not fully reflected the change in society and culture, the importance of media recipients or consumers, and the mediating characteristic of the media. In order to generate further insights for sport media related industry and its society, it is necessary to comprehend the contemporary phenomena of real world situation in new media and sport and to discuss how new media influence sport and how their relationship is changing in managerial context. The purpose of this study is to identify new media cases as distribution channels in sports context and is to develop insights by discussing its role and meaning of new media in sport society. Research design, data, and methodology - The study employed the theory-centered review and case analysis. In order to explain phenomena of the role of new media in contemporary sport and to generate related insights in sport context, the study reviewed the new media cases applied in sport industry and interpreted their meaning by employing medium theory, remediation theory, and new media theory. Results - The study discussed the limitation of prior sport media research and identified the characteristics of sport as new media such as remediating, extending sensory organs, reiterating physical space and electronic space. Conclusions - The study derived the characteristics of sport as new media, in a sport setting, and through sports settings. Findings would assist to comprehend the role of new media in spectating sport and provide insights for sport media study.

Effect of Matrix Metalloproteinases-2 and -9 during IVC-2 on the Development Competence and Gene Expression Profile of Bovine In Vitro-Produced Embryos

  • Lee, Kyeong-Lim;Bang, Jae-Il;Ha, A-Na;Fakruzzaman, Md.;Min, Chan-Sik;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.29 no.2
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    • pp.101-109
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    • 2014
  • Matrix Metalloproteinases (MMP)-2 and -9 are participated in embryo development, implantation, remodeling of epithelial cell and ovulation. The objective of this study is to evaluate an impact of MMP2 and MMP9 on embryonic developmental competence as well as gene expression profiles of in vitro-produced bovine embryos. After in vitro fertilization, embryos of all groups were transferred into IVC-2 medium treated with MMP2 and MMP9 to check the optimum concentration on the basis of embryo development competence and cell numbers. The optimum concentrations for MMP2 and 9 were 1,200 ng/ml and 300 ng/ml. The blastocyst development competence was not different among 1,200 ng/ml of MMP2 vs. 300 ng/ml of MMP9 vs. combined MMP2 + 9 vs. control groups ($41.46{\pm}10.66$ vs. $37.73{\pm}8.92$ vs. $45.11{\pm}11.41%$ vs. $41.59{\pm}11.88$, respectively). Furthermore, the developmental competences to hatching and hatched blastocysts were not also different among the same groups ($79.84{\pm}12.63$ vs. $83.3{\pm}17.46$ vs. $78.55{\pm}14.48%$ vs. $72.02{\pm}14.09$). In addition, total cell number was significantly (p<0.05) greater in blastocyst treated with MMP9 300 ng/ml among all treatment groups. On the other hand, there was no significant difference of ICM vs. TE ratio in all groups. The expression of five out of six genes (i.e., MMP2, MMP9, IFNt, SSLP1 and HNRNPA2B1) was different among the groups. The expression of IFNt and HNRNPA2B1 genes was significantly greater in MMP9 (p<0.05), but there was no difference of MMP9 expression between MMP2 and MMP9 group (p>0.05). The normalized expression of MMP2 and SSLP1 was greater in MMP2 than other groups (p<0.05). In conclusion, MMPs treatment during IVC-2 medium was remarkably effected on blastocyst developmental competence and gene expression profiles that are related to embryo quality and implantation.

Chitosan-coated Packaging Papers for Storage of Agricultural Products (농산물 저장을 위한 키토산코팅 지류 포장재)

  • Yi, Jin-Hee;Kim, Ik-Hwan;Choe, Chan-Ho;Seo, Yung-Bum;Song, Kyung-Bin
    • Applied Biological Chemistry
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    • v.41 no.6
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    • pp.442-446
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    • 1998
  • Chitosan has been known to have an antimicrobial activity. Chitosan samples having different molecular weights were tested for the antimicrobial activity against Escherichia coli in a liquid medium and on the paper sheets coated with different types of chitosan samples. In a liquid medium, Chitosan A $(Mw\;3,000{\sim}5,000)$ and B $(Mw\;200,000{\sim}500,000)$ showed minimal inhibitory concentration (MIC) of 40 and $10\;{\mu}g/ml$, respectively. The MIC of chitosan C $(Mw\;500,000{\sim}1,500,000)$ was similar to that of chitosan B. The paper sheets coated with chitosan A, B, and C also showed growth inhibition against E. coli. As a typical agricultural product, strawberry was tested for the extension of shelf life by using the chitosan-coated packaging papers. Storage experiments showed that chitosan-coated packaging papers extended the shelf life of strawberry at the chilling temperature of $4^{\circ}C$.

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Characteristic of a new variety Lentinula edodes, 'Nongjin-go' (표고 톱밥재배용 신품종 '농진고' 특성)

  • Moon, Ji-Won;Lee, Chan-Jung;Cheong, Jong-chun;Kong, Won-Sik;Kim, Kil-ja
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.228-232
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    • 2015
  • 'Nongjin-go' is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryon Lentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 and verified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growth is $30^{\circ}C$ and it of fruit-body primodium formation is range from 15 to $23^{\circ}C$. Nongjigo is agricultured at mid-high temperature well. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productions per period is regular than 'Sanjo701'. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As its browning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properly for its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.

Morphological Characteristics of Ocean Core Complexes (OCC) in Central Indian Ridge Using High-Resolution Bathymetry and Backscatter Intensity Data from a Deep-Towed Vehicle (심해예인 고해상도 수심 자료와 후방산란 강도 자료를 이용한 인도양 중앙해령 내 Ocean Core Complex 구조의 지형적 특성 분석)

  • Hwang, Gyuha;Kim, Seung-Sep;Son, Seung Kyu;Kim, Jonguk;Ko, Youngtak
    • Ocean and Polar Research
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    • v.42 no.1
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    • pp.49-61
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    • 2020
  • We analyzed the morphological characteristics of OCC (Ocean Core Complexes) in the middle part of the Central Indian Ridge (MCIR) using high-resolution geophysical data recorded on the Deep-Tow SideScan Sonar IMI-30 system. In terms of slope-gradient variations calculated from the high-resolution bathymetry data, the normal faults formed by seafloor spreading were associated generally with slopes > 30° and resulted in high backscatter intensities, which reflect more topographic effects than acoustic medium variation. However, the areas associated with gentle slopes < 10° tend to show the backscatter intensities reflecting the acoustic characteristic of the medium. We show that the detachment faults exposing the OCCs were initiated with high-angle normal faults (58°) exhibiting outward and inward dips of a breakaway zone. In order to examine the spatial distribution of OCC structures, we characterized the transition from magmatic-dominant seafloor with abyssal hills to tectonic-dominant seafloor with OCC using the down-slope direction variation. The slope direction of the seafloor generally tends to be perpendicular to the ridge azimuth in the magmatic-dominant zone, whereas it becomes parallel to the given ridge azimuth near the OCC structures. Therefore, this spatial change of seafloor slope directions indicates that the formation of OCC structures is causally associated with the tectonic-dominant spreading rather than magmatic extension. These results also suggest that the topographical characteristics of seafloor spreading and OCC structures can be distinguished using high-resolution geophysical data. Thus, we propose that the high-resolution bathymetry and backscatter intensity data can help select potential areas of exploitation of hydrothermal deposits in MCIR effectively.

Characteristics and Distribution of Microorganisms in a Rice Straw Compost for Cultivation of Button Mushrooms (Agaricus bisporus) (양송이 재배에서 볏짚 배지의 발효 단계별 관여 미생물의 분포양상 및 특성)

  • Lee, Chan-Jung;Yoo, Young-Mi;Moon, Ji-Won;Cheong, Jong-Chun;Kong, Won-Sik;Kim, Yong-Gyun;Lee, Byung-Eui;Yoon, Min-Ho;Sa, Tong-min
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.43-53
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    • 2017
  • In this study, we analyzed the densities and taxonomic characteristics of various microorganisms that play important roles in Agaricus bisporus culture medium composting, and examined changes in the levels of decomposition-related enzymes secreted by these microorganisms. Various microorganisms such as thermophilic bacteria, actinomycetes, fluorescent Pseudomonas spp., and filamentous bacteria are closely associated with culture medium composts of Agaricus bisporus. The population densities of microorganisms change, and harmful bacteria disappear during thermophilic composting. Psychrobacter sp., Pseudomonas sp., Bacillus sp., and Pseudoxanthomonas sp. accounted for the highest proportion of bacteria in the culture media during outdoor composting, whereas Bacillus sp. and Psychrobacillus sp. were dominant after pasteurization. Cellulose and hemicellulose enzymes of the microorganisms were important at an early stage of rice straw composting and after decomposition of carbon sources, respectively. Microorganisms that secreted these enzymes were present in the second and third turning stage of composting.

'Saemimyeon', a Tongil-Type Medium-Late Maturing Rice Variety with High Amylose ContentUsed for Rice Noodle Preparation (쌀면전용 고아밀로스 중생 통일형 벼 '새미면')

  • Cho, Jun-Hyeon;Lee, Jong-Hee;Park, No-Bong;Son, Young-Bo;Oh, Sung-Hwan;Han, Sang-Ik;Song, You-Chun;Seo, Woo-Duck;Park, Dong-Soo;Nam, Min-Hee;Lee, Ji-Yoon
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.522-528
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    • 2018
  • Saemimyeon, a Tongil type, medium-late maturing rice variety, is especially used for preparing rice noodles. Its high amylose content was developed to fit market demands and to be affordable for rice processing industries. One of the high yielding lines, Milyang181 (Hanareum), was used in the final three-way cross of $IR50^*2$/YR18241-B-B-115-1-1 for yield improvement and cultivation stabilization, including disease resistance. YR24235-10-1-3, a high yielding and compact plant type, was selected and named Milyang278 after yield test at NICS (RDA, Miryang) in 2010. It was subjected to regional yield test at six sites in the middle and southern plain areas of South Korea. Saemimyeon heading occurs on August 12 and is a mid-late maturing cultivar, with resistance to leaf blast, rice stripe virus, and bacterial blight (K1-K3a), but it is susceptible to major diseases and insect pest infestation. Saemimyeon showed a high amylose content of 26.7%, with a relatively low KOH digestion value of 3.5, which are key factors in rice noodles and pasta processing. In the local adaptability tests, the yield of Saemimyeon was 7.08 MT/ha-an increase of approximately 106% compared to that of Dasan. Thus, Saemimyeon is suitable for cultivation in the southern and middle plain areas of South Korea.


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