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http://dx.doi.org/10.7732/kjpr.2014.27.5.540

In Vitro Micropropagation of Polygonatum odoratum cv. Gungangbeaksea  

Kim, Man Bae (Medicinal Plant research Team, Gyeongnam Agricultural Research and Extension Servises)
Park, Chun Geun (Department of Herbal Crop Research, NIHHS, RDA)
Yi, Sookyi (Foundation of Agri. Tech. Commercialization & Transfer)
Han, Bong Hee (Foundation of Agri. Tech. Commercialization & Transfer)
Publication Information
Korean Journal of Plant Resources / v.27, no.5, 2014 , pp. 540-545 More about this Journal
Abstract
The Polygonatum odoratum cv. Gungangbeaksea, bred in Gyeongsangnam-Do Agricutural Research & Extension Service, was cultured in vitro for micropropagate rapidly through the culture of rhizome explants ($5{\times}5mm$). The $7{\times}7mm$ explants of adventitious multi-bud clusters (AMC), obtained through the culture of rhizome explants (MS + 3.0 mg/L BA) were cultured on MS media with BA and TDZ. The shoot multiplication was favorable on the MS medium containing 3.0 mg/L TDZ with 2.8 in shoot number. But the formation of AMC was low in all media tested. The explants of AMC were cultured on MS media containing 1.0~5.0 mg/L TDZ and NAA to multiplicate AMC more. The formation of AMC was a little more stimulated on combined MS media of TDZ and NAA, than that with TDZ alone. The multiplication of shoots and AMC was favorable on MS media with 3.0 mg/L TDZ and 5.0 mg/L NAA, and 5.0 mg/L TDZ and 3.0 mg/L NAA. As the concentration of MS salts increased, the formation of AMC was decreased. But the formation of AMC was more stimulated, as the concentration of sucrose increased to 7%. Therefore, the multiplication of shoots and AMC was suitable on media containing 3.0~5.0 mg/L TDZ and NAA, and 7% of sucrose. The explants of AMC were rooted on media with 3.0 mg/L IBA, or 2.0 mg/L NAA with more than 80% in rooting ratio. The plantlets were treated at $5^{\circ}C$ for 8 weeks, and cultured ex vitro for 8 weeks. The survival ratio of plantlets were 100% in vermiculite, and the mixed soil with perlite 1 volumn and vermiculite 1 volumn.
Keywords
Micropropagation; Sucrose; TDZ;
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