• Mycobiology
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• 제40권2호
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• pp.142-144
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• 2012

During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to $^1H$ NMR measurements and comparison of HPLC retention times with those of authentic compounds, their chemical structures were identified as hispidin and hypholomine B. Compounds (hispidin) 1 and 2 (hypholomine B) inhibited neuraminidase, with $IC_{50}$ values of 13.1 and 0.03 ${\mu}M$, respectively.

• Mycobiology
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• 제45권2호
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• pp.57-63
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• 2017

The species of the genus Chlorociboria Seaver are very common on the forest floor, and can be easily distinguished by small and numerous blue-green fruitbody, especially the blue substrate dyed with xylindein produced by this group. This genus has rather high species diversity in the Southern Hemisphere, while a little attention was paid to this group in East Asia area. During a field survey in South Korea, several Chlorociboria specimens were collected. Based on morphological and phylogenetic analyses, three species of Chlorociboria were reported, including one new record in South Korea and one new record in Jeju Island. The key to the species of Chlorociboria from South Korea is provided.

• Journal of Applied Biological Chemistry
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• 제48권1호
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• pp.29-31
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• 2005

Antimicrobial activities of extracts of Forsythia suspensa fruits and Dendranthema indicum buds and flowers against bacteria; Escherichia coli, Staphyloccus aureus, and Bacillus subtilis, and fungi; Aspergillus flavusn, Rhizopus stolonifer, Penicillium citrinum, Aspergillus niger, and Saccharomyces carlsbergensis, were investigated. The plants were extracted with 70% ethanol and the extracts were used for antimicrobial activity assay. All extracts exhibited significant inhibition activity against microorganisms at concentrations ranged from 1.66 to $100\;{\mu}l/ml$. The inhibition activity by the extract of D. indicum buds was stronger than by the extract of F. suspensa fruits and D. indicum flowers. D. indicum buds showed antimicrobial activity against S. aureus which was comparable to other medicinal plants. F. suspensa fruits and D. indicum flowers was suggested to be valuable sources as antimicrobial ingredients in food industry.

• Advances in Traditional Medicine
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• 제9권1호
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• pp.90-96
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• 2009

The antibacterial activity-guided fractionation of the MeOH extract of seeds of traditional medicinal plant Prunus japonica resulted in the isolation of linoleic acid and cis-11-eicosenoic acids, and their methyl esters. Linoleic acid inhibited the growth of Propionibacterium acnes, the acne-causing anaerobic bacterium, but cis-11-eicosenoic acid, methyl linoleate, and cis-11-eicosenoate were found to be inactive. Together with isolated linoleic acid, authentic saturated and unsaturated fatty acids were also tested against P. acnes with other bacteria and fungi. Most of the unsaturated fatty acids possessed anti-acne (MIC $16-128{\mu}g/mL$) and antimicrobial properties.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.107-109
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• 2008

Medicinal fungi, Phellinus linteus and Inonotus xeranticus, produce a cluster of yellow pigment in their fermentation broth that acts as an important element of biological activity. The pigment is composed of diverse polyphenols with a styrylpyrone moiety, mainly hispidin and its dimers, 3,14'-bihispidinyl, hypholomine B, and 1,1-distyrylpyrylethan. Although dimeric hispidins were proposed to be biosynthesized from two molecules of monomer via oxidative coupling by ligninolytic enzymes, laccase and peroxidase, the details of this process remain unknown. In this preliminary study, we attempted to achieve enzymatic synthesis of the hispidin dimer from hispidin by using commercially available horseradish peroxidase (HRP). Consequently, a hispidin dimer, 3,14'-bihispidinyl, was synthesized, whereas the other dimers, hypholomine B and 1,1-distyrylpyrylethan, were not produced. This result suggested that the oxidative coupling at the C-3 and C-14' positions of hispidins was dominant in the process of dimerization by HRP, and indicated that additional catalysts or substrates would be needed to synthesize other hispidin dimers present in the fungal metabolite.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.760-765
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• 2002

Mucor miehei KCTC 6011, which grew successfully in ginseng-steaming effluents and produced a large amount of chitosan efficiently, was selected from various fungi. Approximately 120 mg of chitosan per g-dry mycelium was maximally produced in 84 h at $25^{\circ}C$ when grown in the ginseng-steaming effluent (pH 8.0) supplemented with 0.5% yeast extract and 0.002% CuSO$_4$. Chitosan produced by Mucor miehei KCTC 6011 was identified by the IR-spectra to have deacety lated approximately 56%. Viscosity and molecular weight of the chitosan were 80 cps and $1.07\times10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizoctonia solani in 60 h.

• 생약학회지
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• 제16권2호
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• pp.61-64
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• 1985

To find new species of Basidiomycetes in Korea, 15 species were collected in the medicinal plants garden of Sook Myung Women’s University during the period from May to November, 1983. Of them, 11 species were identified and classified into 10 genera and seven families. Among these species, Macrocystidia cucumis(Pers. ex Fr.) Heim and Melanoleuca verrucipes (Fr.) Singer were found to be unrecorded species in Korea. Also their two genera are newly found ones in Korea. They were named 'Bahm Saek Min $B\breve{u}s\breve{u}t$' and 'Hin Bol Lock $B\breve{u}s\breve{u}t$' respectively and their characteristics were here reported.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.541-542
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• 2010

• 한국약용작물학회지
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• 제5권3호
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• pp.206-210
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• 1997

6월 하순부터 1개월 간격으로 작약의 잎과 줄기에 인위적 고사를 유도하여 지상부 고사가 뿌리의 발달, 수량, 상품성에 미치는 영향을 구명코자 시험한 결과는 다음과 같다. 1. 작약의 잎과 줄기를 고사시키는 주 병원균은 점무늬병균, 녹병균, 흰가루병균, 검은무늬병균, 탄저병균, 갈색점무늬병원균이었다. 2. 점무늬병과 흰가루병은 4월 하순에서 5월 중순경에 발병되었고, 녹병은 6월 상 순부터 발생되어 시일이 경과할수록 발생율이 증가하였다. 3. 작약 지상부의 고사는 5월 하순부터 시작되어 8월 하순경에는 50% 이상의 고사율을 보였다. 4. 수량은 정상주에 비해 6월 하순 및 7월 하순 고사가 $31{\sim}35%$ 감수되었고, 8월 하순과 9월 하순 고사가 $7{\sim}12%$의 감수를 보였다. 5. 직경 10mm이상 상품성이 있는 뿌리수는 6월과 7월 고사가 8월이후 고사에 비해 적었고 주성분 Paeoniflorin 함량은 조기에 고사된 작약 뿌리 가 오히려 높았다.

• 한국토양비료학회지
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• 제37권5호
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• pp.350-355
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• 2004

본 연구는 분무경 방식을 이용한 arbuscular 균근균 접종원의 대량배양증식을 위하여 기주식물의 종류와 분무양액의 P 농도 등에 따른 균근감염율 및 감염양상 변화 등을 비교하였다. 기주식물 선발에서는 고구마와 토마토가 수단그라스와 질경이에 비해 우수하였다. 또한 인산농도가 5 또는 $10{\mu}M$ 보다 $20{\mu}M$의 수준으로 높아질수록 뿌리의 생성량이 많아진 반면, 뿌리의 균근감염은 인산농도가 낮을수록 높고, 하부 뿌리로 갈수록 arbuscular 균근균 감염율이 낮아지는 경향을 보였으며 감염율은 18.6~26.0% 범위였다. 16주후의 포자형성을 조사한 결과 신선중 1 g 당 500개의 포자가 형성되었고, 분무생육상당 생산된 포자는 총830.479개였다.