• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1749-1759
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• 2018

Recombinant (rec) prion protein (PrP) is an extremely useful resource for studying protein misfolding and subsequent protein aggregation events. Here, we report mass production of high-purity rec-polypeptide encoding the C-terminal globular domain of PrP; (90-230) for human and (89-231) for murine PrP. These proteins were expressed as His-tagged fusion proteins in E. coli cultured by a high cell-density aerobic fermentation method. RecPrPs recovered from inclusion bodies were slowly refolded under reducing conditions. Purification was performed by a sequence of metal-affinity, cation-exchange, and reverse-phase chromatography. The current procedure yielded several dozens of milligrams of recPrP per liter with >95% purity. The purified recPrPs predominantly adopted an ${\alpha}$-helix-rich conformation and were functionally sufficient as substrates to measure the seeding activity of human and animal prions. Establishment of a procedure for high-level production of high-purity recPrP supports the advancement of in vitro investigations of PrP including diagnosis for prion diseases.

• 한국보건간호학회지
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• 제18권1호
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• pp.80-89
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• 2004

This study focused on individual health education for people with hypertension who were being seen in public health centers. The program was an adjusted intervention program that considered the characteristics of each individual and reflected the individual's will to change. Each individual had a different lifestyle with different characteristics and habits so the study considered these individual's characteristics. A comparison was done of knowledge related to hypertension, self-efficacy, and self-care in these individuals. In order to determine the effectiveness of education given by the nurses the variables were measured before and after the individual health education program. The participants in this study were 85 people with hypertension who were seen at a public health center in G city. They were assigned to an experimental group (43) and a control group (42). A tool developed by Park Young-Im (1994) was used to measure knowledge related to hypertension and self-efficacy. A tool developed by Lee Young-Whee (1994) was used for self-care. Data collection was done for 10 weeks from March 24 to May 31, 2003 using interviews with questionnaires. The following is a summary of the results of the study. Hypothesis 1, 'the experimental group which had the individual health education will have higher knowledge scores on hypertension than the control group that did not have the individual health education' was supported (t=4.17, P=0.00). Hypothesis 2, 'the experimental group which had the individual health education will have higher self-efficacy scores than the control group that did not have the individual health education' was supported (t=4.06, P=0.00). Hypothesis 3, 'the experimental group which had the individual health education will have better self-care ability than the control group that did not have the individual health education' was supported (t=4.94, P=0.00). Based on the results of this study, the public health centers should develop an educational program that uses a variety of visual aids and assess the effects of the education on patients with different chronic diseases. After the development of an inclusive education program which will standardize health education effectively and provide a variety of teaching methods, study is needed to measure changes in lifestyle after education and to determine how much knowledge related to hypertension, self-efficacy, and self-care increase

• 지역사회간호학회지
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• 제17권1호
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• pp.26-37
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• 2006

Purpose: This study was to investigate the current situation of community health nursing practicum in bachelor programs. Method: Data were collected using a questionnaire consisting of questions on education goals, teaching items, teaching methods, and evaluation methods and items. Forty five nursing departments/colleges or 84.9% of four-year nursing schools in Korea responded to the survey. Result: Nursing process application and understanding about the role and function of community health nurses were major goals of practice education. Community health centers were the most significant practice fields because all nursing schools mentioned them as places for practice. All nursing schools used a specific evaluation tool to measure students outcomes and utilized guidebooks to help students. Conclusion: The results of this study suggest that the goals of practice education should be standardized to improve the quality of education. Besides, evaluation tools that can be used commonly at all nursing schools should be developed to measure the effectiveness of practice education of community health nursing.

• 한국보육지원학회지
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• 제14권6호
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• pp.241-255
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• 2018

Objective: The purpose of this research was to examine the moderating effect of children's negative emotionality on the relationship between maternal attitude toward children's emotional expression based on the differential susceptibility model. Methods: Participants were 216 preschool children including 116 boys and 100 girls from day care centers and preschools located in Chungchung province and Seoul. EAS was used to measure the children's negative emotionality. The children's empathy quotient was used to measure empathy. Mothers reported their attitude toward children's emotional expression. Results: The results revealed that the association between empathy and maternal suppression of children's emotional expression was significant only for the children with high levels of negative emotionality. Moreover, the magnitude of association between empathy and maternal acceptance of children's emotional expression was greater for high levels of negative emotionality. Conclusion/Implications: It was concluded that these findings supported the differential susceptibility model.

• 한국보육지원학회지
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• 제15권5호
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• pp.69-86
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• 2019

Objective: This study aimed to provide a systematic understanding of children's peer initiative and peer acceptance and to offer specific information to increase young children's social competence. Methods: Participants were 235 5-year-old children from six child care centers in Sejong City. The participants were interviewed to measure the peer acceptance of children attending the same class. Also, a survey was conducted on their teachers to measure children's peer initiative and the relationship with them. The collected data were analyzed by hierarchical regression using the SPSS 21.0 program. Results: The results showed that significant main effects of friendly and unfriendly peer-initiatives on peer acceptance were found. The effects of teacher-child relationships and their moderating effects, however, were partial; only main influences of close relationships and the interaction effects between peer-initiatives and close relationships were significant. That is, the relationships between improper initiatives and low acceptance levels among peers were more intense when children established conflict relationships with their teacher. Conclusion/Implications: We found that close teacher-child relationships play moderating roles on the pathway from peer initiative to peer acceptance as a protective risk factor for children.

• 산업경영시스템학회지
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• 제42권2호
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• pp.9-17
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• 2019

In order to deal with high uncertainty and variability in emergency medical centers, many researchers have developed various models for their operational planning and scheduling. However, most of the models just provide static plans without any risk measures as their results, and thus the users often lose the opportunity to analyze how much risk the patients have, whether the plan is still implementable or how the plan should be changed when an unexpected event happens. In this study, we construct a simulation model combined with a risk-based planning and scheduling module designed by Simio LLC. In addition to static schedules, it provides possibility of treatment delay for each patient as a risk measure, and updates the schedule to avoid the risk when it is needed. By using the simulation model, the users can experiment various scenarios in operations quickly, and also can make a decision not based on their past experience or intuition but based on scientific estimation of risks even in urgent situations. An example of such an operational decision making process is demonstrated for a real mid-size emergency medical center located in Seoul, Republic of Korea. The model is designed for temporal short-term planning especially, but it can be expanded for long-term planning also with some appropriate adjustments.

• 한국보육지원학회지
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• 제17권1호
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• pp.65-84
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• 2021

Objective: The objective of this study was to investigate the mediating effect of children's emotional intelligence on teacher-child intimacy perceived by children and peer acceptance. Methods: The participants were 134 five-year-old children(71 boys, 63 girls) attending daycare centers and kindergarten in Seoul and Gyeong-nam province. Children were interviewed to measure teacher-child intimacy and peer acceptance. Teachers completed rating scales to measure children's emotional intelligence. Data were analyzed using descriptive statistics, Pearson's correlation coefficient, three step mediated regression analysis and a Sobel test. Results: The main results are as follows. first, there were significant positive correlations of teacher-child intimacy, emotional intelligence, and children's peer acceptance. Second, children's emotional intelligence had a partial mediating effect on teacher-child intimacy and peer acceptance. Conclusion/Implications: The results of this study demonstrates links between early teacher-child relationships and children's emotional and social development. In order to promote children's peer acceptance, it is necessary to build an intimate teacher-child relationship and to improve children's emotional intelligence based on that relationship.

• Genomics & Informatics
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• 제6권1호
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• pp.18-28
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• 2008

Single nucleotide polymorphisms (SNPs) are the most abundant forms of human genetic variations and resources for mapping complex genetic traits and disease association studies. We have constructed a linkage disequilibrium (LD) map of chromosome 22 in Korean samples and compared it with those of other populations, including Yorubans in Ibadan, Nigeria (YRI), Centre d'Etude du Polymorphisme Humain (CEPH) reference families (CEU), Japanese in Tokyo (JPT) and Han Chinese in Beijing (CHB) in the HapMap database. We genotyped 4681 of 111,448 publicly available SNPs in 90 unrelated Koreans. Among genotyped SNPs, 4167 were polymorphic. Three hundred and five LD blocks were constructed to make up 18.6% (6.4 of 34.5 Mb) of chromosome 22 with 757 tagSNPs and 815 haplotypes (frequency $\geq$ 5.0%). Of 3430 common SNPs genotyped in all five populations, 514 were monomorphic in Koreans. The CHB + JPT samples have more than a 72% overlap with the monomorphic SNPs in Koreans, while the CEU + YRI samples have less than a 38% overlap. The patterns of hot spots and LD blocks were dispersed throughout chromosome 22, with some common blocks among populations, highly concordant between the three Asian samples. Analysis of the distribution of chimpanzee-derived allele frequency (DAF), a measure of genetic differentiation, Fst levels, and allele frequency difference (AFD) among Koreans and the HapMap samples showed a strong correlation between the Asians, while the CEU and YRI samples showed a very weak correlation with Korean samples. Relative distance as a quantitative measurement based upon DAF, Fst, and AFD indicated that all three Asian samples are very proximate, while CEU and YRI are significantly remote from the Asian samples. Comparative genome-wide LD studies provide useful information on the association studies of complex diseases.

• 대한통합의학회지
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• 제10권2호
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• pp.13-25
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• 2022

Purpose : This study compared the cost-effectiveness ratio of physical therapy in health centers and home physical therapy, two physical therapy methods for home-bound stroke patients, and clarified the economic validity regarding the effect of home physical therapy. Methods : To measure and compare the cost and effectiveness of the two physical therapy methods for stroke patients, subjects were recruited based on in-hospital and home physical therapy. Among the entire data collected, 82 and 90 participants were selected for in-hospital and home physical therapy, respectively. To measure costs, regarding both in-hospital and home physical therapy, direct cost and indirect cost for patients, family, medical institutes, and the government were measured. In addition, activities of daily living were measured in both methods to measure their effectiveness. Through collected data, the cost-effectiveness and incremental cost-effectiveness ratios were analyzed. Results : Based on the analysis of cost-effectiveness, home physical therapy showed lower cost-effectiveness than in-hospital physical therapy. Furthermore, the incremental cost-effectiveness ratio also showed a difference, which implied home physical therapy could have high effectiveness compared to cost. Conclusion : Based on these results, home physical therapy could be considered as an alternativeto other methods of physical therapy, for home-bound stroke patients. In addition, the result of thisstudy contribute by providing evidence that home physical therapy offers economic benefits and canbe more effective in treating home-bound patients when policy decisions are made to establish a home physical therapy system.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -1
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• pp.46-49
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• 2002

One problem of vision-based eye-gazed detection is that it gives low resolution. Base on the displacement of the eyes, we proposed method for vision-based eye-gaze detection. While looking at difference positions on the screen, the distance of the centers of the eyes change accordingly. This relationship is derived and used to map the displacement to the distance in the screen. The experiments are performed to measure the accuracy and resolution to verify the proposed method. The results shown the accuracy of the screen mapping function for the horizontal plane are 76.47% and the error of this function be 23.53%