• Structural Engineering and Mechanics
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• 제8권5호
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• pp.453-464
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• 1999

Safety monitoring systems of structures generally resort to detecting possible changes of dynamic system parameters. Sensitivity analysis of these dynamic system parameters may implement these techniques. Conventional structural eigenvalue problems are discussed in the scope of those systems with deterministic parameters. Large and flexible structures, such as suspension bridges, actually possess stochastic material properties and these random properties unavoidably affect the dynamic system parameters. The sensitivity matrix of structural modal parameters to basic design variables has been established in this paper. Moreover, second order statistics of natural frequencies due to the randomness of material properties have been discussed. It is concluded from numerical analysis of a modem suspension bridge that although the second order statistics of frequencies are small relatively to the change of basic design variables, such as density of mass and modulus of elasticity, the sensitivities of modal parameters to these variables at different locations change in magnitude.

• 생약학회지
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• 제40권1호
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• pp.13-17
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• 2009

It was previously reported that yeast elicitor transiently increased oleanolic acid and ursolic acid in Scutellaria baicalensis suspension cultures and also doubled phospholipase $A_2$ ($PLA_2$) activity. Thus, $PLA_2$ was purified from the soluble fractions of S. baicalensis suspension cultures and the characters of the purified $PLA_2$ were identified. The $PLA_2$ was purified about 160 times compared with the starting soluble-protein extract from S. baicalensis suspension culture cells. The purified protein showed a molecular mass of about 43 kDa by SDS-PAGE. The purified plant $PLA_2$ had a neutral pH optimum (pH 7.0) and required $Ca^{2+}$ for activity. The $PLA_2$ activity was inhibited by mammalian $PLA_2$ inhibitors such as 5,8,11,14-eicosatetraynoic acid(ETYA) and arachidonyl trifluoromethyl ketone ($AACOCF_3$).

• 제어로봇시스템학회논문지
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• 제9권9호
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• pp.669-678
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• 2003

A road-adaptive LQG control for the semi-active Macpherson strut suspension system of hydraulic type is investigated. A new control-oriented model, which incorporates the rotational motion of the unsprung mass, is used for control system design. First, based on the extended least squares estimation algorithm, a LQG controller adapting to the estimated road characteristics is designed. With computer simulations, the performance of the proposed LQC-controlled semi-active suspension is compared with that of a non-adaptive one. The results show better control performance of the proposed system over the compared one.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2011년도 추계학술대회 논문집
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• pp.343-348
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• 2011

This paper presents ride comfort characteristics of a quarter-vehicle magneto-rheological (MR) suspension system with respect to different tire pressure. As a first step, controllable MR damper is designed and modeled based on both the optimized damping force levels and mechanical dimensions required for a commercial full-size passenger vehicle. Then, a quarter-vehicle suspension system consisting of sprung mass, spring, tire and the MR damper is constructed. After deriving the equations of the motion for the proposed quarter-vehicle MR suspension system, vertical tire stiffness with respect to different tire pressure is experimentally identified. The skyhook controller is then implemented for the realization of the quarter-vehicle MR suspension system. Finally, the ride comfort analysis with respect to different tire pressure is undertaken in time domain. In addition, a comparative result between controlled and uncontrolled is provided by presenting vertical RMS displacement.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.47.5-47
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• 2001

In this paper. a road-adaptive LQG control for the semiactive Macpherson strut suspension system of hydraulic type is investigated. A new control oriented model, which incorporates the rotational motion of the unsprung mass, is introduced. A semi-active suspension controller adapting to road variations is proposed. First, based on the extended least squares estimation algorithm, a LQG controller adapting to the estimated road characteristics is designed. Through the computer simulations, the performance of the proposed semi-active suspension is compared with that of a non-adaptive one. The results show better control performance of the proposed system over the compared one.

• 한국자동차공학회논문집
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• 제2권3호
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• pp.50-61
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• 1994

A method for performing dynamic design sensitivity analysis of vehicle suspension systems which have three dimensional closed-loop kinematic structure is presented. A recursive form of equations of motion for a MacPherson suspension system is derived as basis for sensitivity analysis. By directly differentiating the equations of motion with respect to design variables, sensitivity equations are obtained. The direct generalize for the application of multibody dynamic sensitivity analysis. Based on the proposed sensitivity analysis, optimal design of a MacPherson suspension system is carried out taking unsprung mass, spring and damping coefficients as design variables.

• Wind and Structures
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• 제10권1호
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• pp.1-22
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• 2007

The suppression of aerodynamic response of long-span suspension bridges during erection and after completion by using single TMD and multi TMD is presented in this paper. An advanced finite-element-based aerodynamic model that can be used to analyze both flutter instability and buffeting response in the time domain is also proposed. The frequency-dependent flutter derivatives are transferred into a time-dependent rational function, through which the coupling effects of three-dimensional aerodynamic motions under gusty winds can be accurately considered. The modal damping of a structure-TMD system is analyzed by the state-space approach. The numerical examples are performed on the Akashi Kaikyo Bridge with a main span of 1990 m. The bridge is idealized by a three-dimensional finite-element model consisting of 681 nodes. The results show that when the wind velocity is low, about 20 m/s, the multi TMD type 1 (the vertical and horizontal TMD with 1% mass ratio in each direction together with the torsional TMD with ratio of 1% mass moment of inertia) can significantly reduce the buffeting response in vertical, horizontal and torsional directions by 8.6-13%. When the wind velocity increases to 40 m/s, the control efficiency of a multi TMD in reducing the torsional buffeting response increases greatly to 28%. However, its control efficiency in the vertical and horizontal directions reduces. The results also indicate that the critical wind velocity for flutter instability during erection is significantly lower than that of the completed bridge. By pylon-to-midspan configuration, the minimum critical wind velocity of 57.70 m/s occurs at stage of 85% deck completion.

• Reproductive and Developmental Biology
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• 제36권1호
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• pp.21-26
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• 2012

Suspension culture is a useful tool for culturing embryonic stem (ES) cells in large-scale, but the stability of pluripotency and karyotype has to be maintained $in$ $vitro$ for clinical application. Therefore, we investigated whether the chromosomal abnormality of ES cells was induced in suspension culture or not. The ES cells were cultured in suspension as a form of aggregate with or without mouse embryonic fibroblasts (MEFs), and 0 or 1,000 U/ml leukemia inhibitory factor (LIF) was treated to suspended ES cells. After culturing ES cells in suspension, their karyotype, DNA content, and properties of pluripotency and differentiation were evaluated. As a result, the formation of tetraploid ES cell population was significantly increased in suspension culture in which ES cells were co-cultured with both MEFs and LIF. Tetraploid ES cell population was also generated when ES cells were cultured alone in suspension regardless of the existence of LIF. On the other hand, the formation of tetraploid ES cell population was not detected in LIF-free condition, in which MEFs were included. The origin of tetraploid ES cell population was turned out to be E14 ES cells and not MEFs by microsatellite analysis and the basic properties of them were still maintained despite ploidy-conversion to tetraploidy. Furthermore, we identified the ploidy shift from tetraploidy to near-triploidy as tetraploid ES cells were differentiated spontaneously. From these results, we demonstrated that suspension culture system could induce ploidy-conversion generating tetraploid ES cell population. Moreover, optimization of suspension culture system may make possible mass-production of ES cells.

• 한국기계가공학회지
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• 제16권6호
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• pp.47-54
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• 2017

This study analyzed air suspension seat frame structure and vibration for 50 - 180 kg mass driver to obtain optimum seat design parameter values for the equivalent spring constant and damping coefficient. Various air suspension seat frames were designed following WTS-003 and KS B 6839 standards, and then evaluated using finite elements analysis. Resonance and vibration tests were performed according to the 78/764/EEC standard.

• Journal of Korean Biological Nursing Science
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• 제1권1호
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• pp.71-85
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• 1999

The purpose of this study was to determine the effect of periodic low-intensity exercise during hindlimb suspension on the mass, relative weight, myofibrillar protein content in soleus, plantaris and gastrocnemius muscles. To examine the effectiveness of periodic low-intensity exercise on mass, and myofibrillar protein content of hindlimb muscles, adult female Wistar rats were suspended(HS) and half of these rats walked on a treadmill for 45min/day(15 min every 4h) at 5m/min and a $15^{\circ}$ grade(HS-EX). Soleus wet weight was 33.51% significantly smaller(p<0.005) and relative soleus weight of hindlimb suspended rats was 31.96% smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Plantaris wet weight was 7.5% smaller(p<0.01) and relative plantaris weight was 11.83% smaller(p<0.05) compared with those of control rats following seven days of hindlimb suspension. Gastrocnemius wet weight was 11.31% significantly smaller(p<0.005) and relative gastrocnemius weight was 17.13% significantly smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Soleus wet weight while increased by relative soleus weight increased by 25.13%, 27.59% each through periodic low intensity exercise during hindlimb suspension(p<0.05, p<0.05). Plantaris wet weight and relative plantaris weight increased by 1.04%, 10.98%(p<0.05) each, and gastrocnemius wet weight and relative gastrocnemius weight increased by 1.98%, 12.02%(p<0.05) each through periodic low intensity exercise during hindlimb suspension. Wet weight of soleus, plantaris and gastrocnemius in HS-EX rats did not recover to control level. Myofibrillar protein content of soleus, plantaris and gastrocnemius was 48.24%, 40.85% and 37.33% significantly smaller(p<0.005) respectively compared with those of control rats following seven days of hindlimb suspension. Myofibrillar protein content of soleus, plantaris and gastrocnemius increased by 40.68%, 25.07% and 17.93%(p<0.005) each through periodic low intensity exercise during hindlimb suspension. Myofibrillar protein content of soleus, plantaris, and gastrocnemius in HS-EX rats did not recover to control level. The results suggest that periodic low intensity exercise can attenuate hindlimb muscle atrophy induced by hindlimb suspension.